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Development of stable homozygous wheat/Amblyopyrum muticum (Aegilops mutica) introgression lines and their cytogenetic and molecular characterization (2019)
Journal Article
King, J., Newell, C., Grewal, S., Hubbart-Edwards, S., Yang, C., Scholefield, D., …King, I. P. (2019). Development of stable homozygous wheat/Amblyopyrum muticum (Aegilops mutica) introgression lines and their cytogenetic and molecular characterization. Frontiers in Plant Science, 10, 1-10. https://doi.org/10.3389/fpls.2019.00034

Wheat is one of the world’s most important sources of food. However, due to its evolution its genetic base has narrowed, which is severely limiting the ability of breeders to develop new higher yielding varieties that can adapt to the changing enviro... Read More about Development of stable homozygous wheat/Amblyopyrum muticum (Aegilops mutica) introgression lines and their cytogenetic and molecular characterization.

Developing a high-throughput SNP-based marker system to facilitate the introgression of traits from Aegilops species into bread wheat (Triticum aestivum) (2019)
Journal Article
Przewieslik-Allen, A. M., Burridge, A. J., Wilkinson, P. A., Winfield, M. O., Shaw, D. S., McAusland, L., …Barker, G. L. (2019). Developing a high-throughput SNP-based marker system to facilitate the introgression of traits from Aegilops species into bread wheat (Triticum aestivum). Frontiers in Plant Science, 9, Article 1993. https://doi.org/10.3389/fpls.2018.01993

The genus Aegilops contains a diverse collection of wild species exhibiting variation in geographical distribution, ecological adaptation, ploidy and genome organization. Aegilops is the most closely related genus to Triticum which includes cultivate... Read More about Developing a high-throughput SNP-based marker system to facilitate the introgression of traits from Aegilops species into bread wheat (Triticum aestivum).

An investigation of genotype-phenotype association in a festulolium forage grass population containing genome-spanning Festuca pratensis chromosome segments in a Lolium perenne background (2018)
Journal Article
Harper, J., Gasior, D., Mathews, R., Thomas, A., Evans, C., King, J., …Armstead, I. (2018). An investigation of genotype-phenotype association in a festulolium forage grass population containing genome-spanning Festuca pratensis chromosome segments in a Lolium perenne background. PLoS ONE, 13(11), Article e0207412. https://doi.org/10.1371/journal.pone.0207412

Alien chromosome introgression is used for the transfer of beneficial traits in plant breeding. For temperate forage grasses, much of the work in this context has focused on species within the ryegrasses (Lolium spp.) and the closely related fescues... Read More about An investigation of genotype-phenotype association in a festulolium forage grass population containing genome-spanning Festuca pratensis chromosome segments in a Lolium perenne background.

Detection of T. urartu introgressions in wheat and development of a panel of interspecific introgression lines (2018)
Journal Article
Grewal, S., Edwards, S. H., Yang, C., Scholefield, D., Ashling, S., Burridge, A., …King, J. (2018). Detection of T. urartu introgressions in wheat and development of a panel of interspecific introgression lines. Frontiers in Plant Science, 9, https://doi.org/10.3389/fpls.2018.01565

Tritcum urartu (2n = 2x = 14, AuAu), the A genome donor of wheat, is an important source for new genetic variation for wheat improvement due to its high photosynthetic rate and disease resistance. By facilitating the generation of genome-wide introgr... Read More about Detection of T. urartu introgressions in wheat and development of a panel of interspecific introgression lines.

B chromosomes are associated with redistribution of genetic recombination towards lower-recombination chromosomal regions in perennial ryegrass (2018)
Journal Article
Harper, J., Phillips, D., Thomas, A., Gasior, D., Evans, C., Powell, W., …Armstead, I. (2018). B chromosomes are associated with redistribution of genetic recombination towards lower-recombination chromosomal regions in perennial ryegrass. Journal of Experimental Botany, 69(8), https://doi.org/10.1093/jxb/ery052

Supernumerary ‘B’ chromosomes are non-essential components of the genome present in a range of plant and animal species – including many grasses. Within diploid and polyploid ryegrass and fescue species, including the forage grass perennial ryegrass... Read More about B chromosomes are associated with redistribution of genetic recombination towards lower-recombination chromosomal regions in perennial ryegrass.

Introgression of Aegilops speltoides segements in Triticum aestivum and the effect of the gametocidal genes (2017)
Journal Article
King, J., Grewal, S., Yang, C., Hubbart Edwards, S., Scholefield, D., Ashling, S., …King, I. P. (2018). Introgression of Aegilops speltoides segements in Triticum aestivum and the effect of the gametocidal genes. Annals of Botany, 121(12), https://doi.org/10.1093/aob/mcx149

• Background and Aims Bread wheat (Triticum aestivum) has been through a severe genetic bottleneck as a result of its evolution and domestication. It is therefore essential that new sources of genetic variation are generated and utilized. This study... Read More about Introgression of Aegilops speltoides segements in Triticum aestivum and the effect of the gametocidal genes.

Characterisation of Thinopyrum bessarabicum chromosomes through genome-wide introgressions into wheat (2017)
Journal Article
Grewal, S., Yang, C., Hubbart Edwards, S., Scholefield, D., Ashling, S., Burridge, A., …King, J. (2018). Characterisation of Thinopyrum bessarabicum chromosomes through genome-wide introgressions into wheat. TAG Theoretical and Applied Genetics, 131(2), 389–406. https://doi.org/10.1007/s00122-017-3009-y

Thinopyrum bessarabicum (2n = 2x = 14, JJ) is an important source for new genetic variation for wheat improvement due to its salinity tolerance and disease resistance. Its practical utilisation in wheat improvement can be facilitated through developm... Read More about Characterisation of Thinopyrum bessarabicum chromosomes through genome-wide introgressions into wheat.

Comparative mapping and targeted-capture sequencing of the gametocidal loci in Aegilops sharonensis (2017)
Journal Article
Grewal, S., Gardiner, L., Ndreca, B., Knight, E., Moore, G., King, I. P., & King, J. (2017). Comparative mapping and targeted-capture sequencing of the gametocidal loci in Aegilops sharonensis. Plant Genome, The, 10(2), https://doi.org/10.3835/plantgenome2016.09.0090

Gametocidal (Gc) chromosomes or elements in species such as Aegilops sharonensis are preferentially transmitted to the next generation through both the male and female gametes when introduced into wheat. Furthermore, any genes, e.g. genes that contro... Read More about Comparative mapping and targeted-capture sequencing of the gametocidal loci in Aegilops sharonensis.

Wheat landrace genome diversity (2017)
Journal Article
Wingen, L. U., West, C., Leverington-Waite, M., Collier, S., Orford, S., Goram, R., …Griffiths, S. (2017). Wheat landrace genome diversity. Genetics, 205(4), https://doi.org/10.1534/genetics.116.194688

Understanding the genomic complexity of bread wheat (Triticum aestivum L.) is a cornerstone in the quest to unravel the processes of domestication and the following adaptation of domesticated wheat to a wide variety of environments across the globe.... Read More about Wheat landrace genome diversity.

A step change in the transfer of interspecific variation into wheat from Amblyopyrum muticum (2016)
Journal Article
King, J., Grewal, S., Yang, C., Hubbart, S., Scholefield, D., Ashling, S., …King, I. P. (2017). A step change in the transfer of interspecific variation into wheat from Amblyopyrum muticum. Plant Biotechnology Journal, 15(2), 217-226. https://doi.org/10.1111/pbi.12606

Despite some notable successes, only a fraction of the genetic variation available in wild relatives has been utilized to produce superior wheat varieties. This is as a direct result of the lack of availability of suitable high-throughput technologie... Read More about A step change in the transfer of interspecific variation into wheat from Amblyopyrum muticum.

High-density SNP genotyping array for hexaploid wheat and its secondary and tertiary gene pool (2015)
Journal Article
Winfield, M. O., King, I., Allen, A. M., Burridge, A. J., Barker, G. L. A., Benbow, H. R., …Edwards, K. J. (2016). High-density SNP genotyping array for hexaploid wheat and its secondary and tertiary gene pool. Plant Biotechnology Journal, 14(5), 1195-1206. https://doi.org/10.1111/pbi.12485

In wheat, a lack of genetic diversity between breeding lines has been recognized as a significant block to future yield increases. Species belonging to bread wheat's secondary and tertiary gene pools harbour a much greater level of genetic variabilit... Read More about High-density SNP genotyping array for hexaploid wheat and its secondary and tertiary gene pool.

Generation of amphidiploids from hybrids of wheat and related species from the genera Aegilops, Secale, Thinopyrum and Triticum as a source of genetic variation for wheat improvement (2015)
Journal Article
Nemeth, C., Yang, C., Kasprzak, P., Hubbart, S., Scholefield, D., Mehra, S., …King, J. (2015). Generation of amphidiploids from hybrids of wheat and related species from the genera Aegilops, Secale, Thinopyrum and Triticum as a source of genetic variation for wheat improvement. Genome, 58(2), 71-79. https://doi.org/10.1139/gen-2015-0002

We aim to improve diversity of domesticated wheat by transferring genetic variation for important target traits from related wild and cultivated grass species. The present study describes the development of F1 hybrids between wheat and related specie... Read More about Generation of amphidiploids from hybrids of wheat and related species from the genera Aegilops, Secale, Thinopyrum and Triticum as a source of genetic variation for wheat improvement.

Phenotyping pipeline reveals major seedling root growth QTL in hexaploid wheat (2015)
Journal Article
Atkinson, J. A., Wingen, L. U., Griffiths, M., Pound, M. P., Gaju, O., Foulkes, M. J., …Wells, D. M. (2015). Phenotyping pipeline reveals major seedling root growth QTL in hexaploid wheat. Journal of Experimental Botany, 66(8), 2283-2292. https://doi.org/10.1093/jxb/erv006

Seedling root traits of wheat (Triticum aestivum L.) have been shown to be important for efficient establishment and linked to mature plant traits such as height and yield. A root phenotyping pipeline, consisting of a germination paper-based screen c... Read More about Phenotyping pipeline reveals major seedling root growth QTL in hexaploid wheat.

Transmission Frequencies of Introgressed Festuca pratensis Chromosomes and Chromosome Segments in Lolium perenne (2013)
Journal Article
King, J., Armstead, I., Harper, J., & King, I. (2013). Transmission Frequencies of Introgressed Festuca pratensis Chromosomes and Chromosome Segments in Lolium perenne. Crop Science, 53(5), 1968-1973. doi:10.2135/cropsci2013.03.0209

The introgression of genetic variation from related species into crops provides an important route by which superior plant varieties can be produced. The primary aim of introgression involves the transfer of a small chromosome segment from a related... Read More about Transmission Frequencies of Introgressed Festuca pratensis Chromosomes and Chromosome Segments in Lolium perenne.

A DArT marker genetic map of perennial ryegrass (Lolium perenne L.) integrated with detailed comparative mapping information; comparison with existing DArT marker genetic maps of Lolium perenne, L. multiflorum and Festuca pratensis (2013)
Journal Article
King, J., Thomas, A., James, C., King, I., & Armstead, I. (2013). A DArT marker genetic map of perennial ryegrass (Lolium perenne L.) integrated with detailed comparative mapping information; comparison with existing DArT marker genetic maps of Lolium perenne, L. multiflorum and Festuca pratensis. BMC Genomics, 14(1), https://doi.org/10.1186/1471-2164-14-437

Background Ryegrasses and fescues (genera, Lolium and Festuca) are species of forage and turf grasses which are used widely in agricultural and amenity situations. They are classified within the sub-family Pooideae and so are closely related to Brac... Read More about A DArT marker genetic map of perennial ryegrass (Lolium perenne L.) integrated with detailed comparative mapping information; comparison with existing DArT marker genetic maps of Lolium perenne, L. multiflorum and Festuca pratensis.

Exploitation of interspecific diversity for monocot crop improvement (2013)
Journal Article
King, J., Armstead, I., Harper, J., Ramsey, L., Snape, J., Waugh, R., …King, I. (2013). Exploitation of interspecific diversity for monocot crop improvement. Heredity, 110(5), 475-483. https://doi.org/10.1038/hdy.2012.116

In many cultivated crop species there is limited genetic variation available for the development of new higher yielding varieties adapted to climate change and sustainable farming practises. The distant relatives of crop species provide a vast and la... Read More about Exploitation of interspecific diversity for monocot crop improvement.

Alien introgression in the grasses Lolium perenne (perennial ryegrass) and Festuca pratensis (meadow fescue): The development of seven monosomic substitution lines and their molecular and cytological characterization (2011)
Journal Article
Harper, J., Armstead, I., Thomas, A., James, C., Gasior, D., Bisaga, M., …King, J. (2011). Alien introgression in the grasses Lolium perenne (perennial ryegrass) and Festuca pratensis (meadow fescue): The development of seven monosomic substitution lines and their molecular and cytological characterization. Annals of Botany, 107(8), 1313-1321. https://doi.org/10.1093/aob/mcr083

BACKGROUND AND AIMS: To address the issues associated with food security, environmental change and bioenergy in the context of crop plants, the production, identification and evaluation of novel plant phenotypes is fundamental. One of the major rout... Read More about Alien introgression in the grasses Lolium perenne (perennial ryegrass) and Festuca pratensis (meadow fescue): The development of seven monosomic substitution lines and their molecular and cytological characterization.

Development of intron-flanking EST markers for the Lolium/Festuca complex using rice genomic information (2009)
Journal Article
Tamura, K., Yonemaru, J., Hisano, H., Kanamori, H., King, J., King, I. P., …Yamada, T. (2009). Development of intron-flanking EST markers for the Lolium/Festuca complex using rice genomic information. TAG Theoretical and Applied Genetics, 118(8), 1549-1560. https://doi.org/10.1007/s00122-009-1003-8

DNA markers able to distinguish species or genera with high specificity are valuable in the identification of introgressed regions in interspecific or intergeneric hybrids. Intergeneric hybridization between the genera of Lolium and Festuca, leading... Read More about Development of intron-flanking EST markers for the Lolium/Festuca complex using rice genomic information.

Rice pseudomolecule-anchored cross-species DNA sequence alignments indicate regional genomic variation in expressed sequence conservation (2007)
Journal Article
Armstead, I., Huang, L., King, J., Ougham, H., Thomas, H., & King, I. (2007). Rice pseudomolecule-anchored cross-species DNA sequence alignments indicate regional genomic variation in expressed sequence conservation. BMC Genomics, 8(1), Article 283. https://doi.org/10.1186/1471-2164-8-283

Background Various methods have been developed to explore inter-genomic relationships among plant species. Here, we present a sequence similarity analysis based upon comparison of transcript-assembly and methylation-filtered databases from five plan... Read More about Rice pseudomolecule-anchored cross-species DNA sequence alignments indicate regional genomic variation in expressed sequence conservation.

Introgression mapping in the grasses (2007)
Journal Article
King, J., Armstead, I. P., Donnison, I. S., Harper, J. A., Roberts, L. A., Thomas, H., …King, I. P. (2007). Introgression mapping in the grasses. Chromosome Research, 15(1), 105-113. https://doi.org/10.1007/s10577-006-1103-0

The unique properties of Lolium/Festuca hybrids and their derivatives provide an ideal system for intergeneric introgression. At IGER a focus on the Lolium perenne/Festuca pratensis system is being exploited to elucidate genome organization in the gr... Read More about Introgression mapping in the grasses.