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Fast Raman spectral mapping of highly fluorescing samples by time-gated spectral multiplexed detection (2018)
Journal Article
Corden, C. J., Shipp, D. W., Matousek, P., & Notingher, I. (2018). Fast Raman spectral mapping of highly fluorescing samples by time-gated spectral multiplexed detection. Optics Letters, 43(23), 5733-5736. https://doi.org/10.1364/ol.43.005733

We present a time-gated Raman micro-spectroscopy technique suitable for fast Raman mapping of samples eliciting large laser-induced fluorescence backgrounds. To achieve the required time resolution for effective fluorescence rejection, a picosecond p... Read More about Fast Raman spectral mapping of highly fluorescing samples by time-gated spectral multiplexed detection.

Holographic optical trapping Raman micro-spectroscopy for non-invasive measurement and manipulation of live cells (2018)
Journal Article
Sinjab, F., Awuah, D., Gibson, G., Padgett, M., Ghaemmaghami, A. M., & Notingher, I. (2018). Holographic optical trapping Raman micro-spectroscopy for non-invasive measurement and manipulation of live cells. Optics Express, 26(19), 25211-25225. https://doi.org/10.1364/oe.26.025211

We present a new approach for combining holographic optical tweezers with confocal Raman spectroscopy. Multiple laser foci, generated using a liquid-crystal spatial light modulator, are individually used for both optical trapping and excitation of sp... Read More about Holographic optical trapping Raman micro-spectroscopy for non-invasive measurement and manipulation of live cells.

Optical sectioning in multi-foci Raman hyperspectral imaging (2018)
Journal Article
Liao, Z., Sinjab, F., Elsheikha, H. M., & Notingher, I. (2018). Optical sectioning in multi-foci Raman hyperspectral imaging. Journal of Raman Spectroscopy, 49(10), 1660-1667. https://doi.org/10.1002/jrs.5450

In this study, we compared the depth-discrimination and speed performance of multi-foci Raman hyperspectral imaging with the reference standard of a single laser point confocal Raman mapping. A liquid crystal spatial light modulator (LC-SLM) was empl... Read More about Optical sectioning in multi-foci Raman hyperspectral imaging.

Intra-operative spectroscopic assessment of surgical margins during breast conserving surgery (2018)
Journal Article
Shipp, D. W., Rakha, E. A., Koloydenko, A. A., Macmillan, R. D., Ellis, I. O., & Notingher, I. (2018). Intra-operative spectroscopic assessment of surgical margins during breast conserving surgery. Breast Cancer Research, 20, https://doi.org/10.1186/s13058-018-1002-2

Background: In over 20% of breast conserving operations, postoperative pathological assessment of the excised tissue reveals positive margins, requiring additional surgery. Current techniques for intra-operative assessment of tumor margins are insuff... Read More about Intra-operative spectroscopic assessment of surgical margins during breast conserving surgery.

Holographic optical trapping Raman micro-spectroscopy for interacting live cells (2018)
Working Paper
Sinjab, F., Elsheikha, H. M., Awuah, D., Gibson, G., Padgett, M., Ghaemmaghami, A. M., & Notingher, I. Holographic optical trapping Raman micro-spectroscopy for interacting live cells

We present a combined holographic optical tweezers and confocal Raman spectroscopy instrument which allows fast, flexible, and interactive manipulation with molecular measurement of interacting live cell systems. Multiple laser foci created using a s... Read More about Holographic optical trapping Raman micro-spectroscopy for interacting live cells.

Visualizing the interaction of Acanthamoeba castellanii with human retinal epithelial cells by spontaneous Raman and CARS imaging (2017)
Journal Article
Naemat, A., Sinjab, F., McDonald, A., Downes, A., Elfick, A., Elsheikha, H. M., & Notingher, I. (2018). Visualizing the interaction of Acanthamoeba castellanii with human retinal epithelial cells by spontaneous Raman and CARS imaging. Journal of Raman Spectroscopy, 49(3), 412-423. https://doi.org/10.1002/jrs.5296

Improved understanding of the mechanism of nutrient’s uptake can enable targeted manipulation of nutrient sensing pathways in medically important pathogens to a greater degree than is currently possible. In this context, we present the use of spontan... Read More about Visualizing the interaction of Acanthamoeba castellanii with human retinal epithelial cells by spontaneous Raman and CARS imaging.

Automated multimodal spectral histopathology for quantitative diagnosis of residual tumour during basal cell carcinoma surgery (2017)
Journal Article
Boitor, R., Kong, K., Shipp, D., Varma, S., Koloydenko, A., Kusum, K., …Ioan, N. (in press). Automated multimodal spectral histopathology for quantitative diagnosis of residual tumour during basal cell carcinoma surgery. Biomedical Optics Express, 8(12), https://doi.org/10.1364/BOE.8.005749

Multimodal spectral histopathology (MSH), an optical technique combining tissue auto-fluorescence (AF) imaging and Raman micro-spectroscopy (RMS), was previously proposed for detection of residual basal cell carcinoma (BCC) at the surface of surgical... Read More about Automated multimodal spectral histopathology for quantitative diagnosis of residual tumour during basal cell carcinoma surgery.

Label-free Raman hyperspectral imaging of single cells cultured on polymer substrates (2017)
Journal Article
Sinjab, F., Sicilia, G., Shipp, D., Marlow, M., & Notingher, I. (2017). Label-free Raman hyperspectral imaging of single cells cultured on polymer substrates. Applied Spectroscopy, 71(12), https://doi.org/10.1177/0003702817715042

While Raman hyper-spectral imaging has been widely used for label-free mapping of biomolecules in cells, these measurements require the cells to be cultured on weakly Raman scattering substrates. However, many applications in biological sciences and... Read More about Label-free Raman hyperspectral imaging of single cells cultured on polymer substrates.

Rapid production of human liver scaffolds for functional tissue engineering by high shear stress oscillation-decellularization (2017)
Journal Article
Mazza, G., Al-Akkad, W., Telese, A., Longato, L., Urbani, L., Robinson, B., …Pinzani, M. (in press). Rapid production of human liver scaffolds for functional tissue engineering by high shear stress oscillation-decellularization. Scientific Reports, 7, Article 5534. https://doi.org/10.1038/s41598-017-05134-1

The development of human liver scaffolds retaining their 3-dimensional structure and extra-cellular matrix (ECM) composition is essential for the advancement of liver tissue engineering. We report the design and validation of a new methodology for th... Read More about Rapid production of human liver scaffolds for functional tissue engineering by high shear stress oscillation-decellularization.

Raman spectroscopy: techniques and applications in the life sciences (2017)
Journal Article
Shipp, D., Sinjab, F., & Notingher, I. (2017). Raman spectroscopy: techniques and applications in the life sciences. Advances in Optics and Photonics, 9(2), 315-428. https://doi.org/10.1364/AOP.9.000315

Raman spectroscopy is an increasingly popular technique in many areas including biology and medicine. It is based on Raman scattering, a phenomenon in which incident photons lose or gain energy via interactions with vibrating molecules in a sample. T... Read More about Raman spectroscopy: techniques and applications in the life sciences.

Feasibility of spatially-offset Raman spectroscopy for in-vitro and in-vivo monitoring mineralisation of bone tissue-engineering scaffolds (2016)
Journal Article
Liao, Z., Sinjab, F., Nommeots-Nomm, A., Jones, J., Ruiz-Cantu, L., Yang, J., …Notingher, I. (2017). Feasibility of spatially-offset Raman spectroscopy for in-vitro and in-vivo monitoring mineralisation of bone tissue-engineering scaffolds. Analytical Chemistry, 89(1), 847-853. https://doi.org/10.1021/acs.analchem.6b03785

We investigated the feasibility of using spatially-offset Raman spectroscopy (SORS) for non-destructive characterisation of bone tissue engineering scaffolds. The deep regions of these scaffolds, or scaffolds implanted subcutaneously in live animals,... Read More about Feasibility of spatially-offset Raman spectroscopy for in-vitro and in-vivo monitoring mineralisation of bone tissue-engineering scaffolds.

In-situ fabrication of gold nanoparticle functionalized probes for tip-enhanced Raman spectroscopy by dielectrophoresis (2016)
Journal Article
Sweetenham, C. S., Woolley, R., & Notingher, I. (2016). In-situ fabrication of gold nanoparticle functionalized probes for tip-enhanced Raman spectroscopy by dielectrophoresis. Journal of Nanophotonics, 10(3), 1-7. https://doi.org/10.1117/1.JNP.10.030502

We report the use of dielectrophoresis to fabricate in-situ probes for tip-enhanced Raman spectroscopy (TERS) based on Au nanoparticles. A typical conductive atomic force microscope (AFM) was used to functionalize iridium-coated conductive silicon pr... Read More about In-situ fabrication of gold nanoparticle functionalized probes for tip-enhanced Raman spectroscopy by dielectrophoresis.

Tissue diagnosis using power-sharing multifocal Raman micro-spectroscopy and auto-fluorescence imaging (2016)
Journal Article
Sinjab, F., Kong, K., Gibson, G., Varma, S., Williams, H. C., Padgett, M., & Notingher, I. (2016). Tissue diagnosis using power-sharing multifocal Raman micro-spectroscopy and auto-fluorescence imaging. Biomedical Optics Express, 7(8), 2993–3006. https://doi.org/10.1364/BOE.7.002993

We describe a multifocal Raman micro-spectroscopy detection method based on a digital micromirror device, which allows for simultaneous “power-sharing” acquisition of Raman spectra from ad hoc sampling points. As the locations of the points can be ra... Read More about Tissue diagnosis using power-sharing multifocal Raman micro-spectroscopy and auto-fluorescence imaging.

DMD-based software-configurable spatially-offset Raman spectroscopy for spectral depth-profiling of optically turbid samples (2016)
Journal Article
Liao, Z., Sinjab, F., Gibson, G., Padgett, M., & Notingher, I. (2016). DMD-based software-configurable spatially-offset Raman spectroscopy for spectral depth-profiling of optically turbid samples. Optics Express, 24(12), 12701-12712. https://doi.org/10.1364/OE.24.012701

Spectral depth-profiling of optically turbid samples is of high interest to a broad range of applications. We present a method for measuring spatially-offset Raman spectroscopy (SORS) over a range of length scales by incorporating a digital micro-mir... Read More about DMD-based software-configurable spatially-offset Raman spectroscopy for spectral depth-profiling of optically turbid samples.

Tracking amino acid’s uptake into the protozoan Acanthamoeba castellanii by stable-isotope labelling and Raman spectral imaging (2016)
Journal Article
Naemat, A., Elsheikha, H. M., & Notingher, I. (2016). Tracking amino acid’s uptake into the protozoan Acanthamoeba castellanii by stable-isotope labelling and Raman spectral imaging. Proceedings of SPIE, 9887, Article 988715. https://doi.org/10.1117/12.2227070

The capacity of pathogens to acquire nutrients from their host cells is one of the most fundamental aspects of infection biology. Hence, measuring the patterns of nutrients’ uptake by pathogens is essential for understanding the interactions of patho... Read More about Tracking amino acid’s uptake into the protozoan Acanthamoeba castellanii by stable-isotope labelling and Raman spectral imaging.

Surface-enhanced Raman spectroscopy study of 4-ATP on gold nanoparticles for basal cell carcinoma fingerprint detection (2016)
Journal Article
Quynh, L. M., Nam, N. H., Kong, K., Nhung, N. T., Notingher, I., Henini, M., & Luong, N. H. (2016). Surface-enhanced Raman spectroscopy study of 4-ATP on gold nanoparticles for basal cell carcinoma fingerprint detection. Journal of Electronic Materials, 45(5), 2563-2568. https://doi.org/10.1007/s11664-016-4421-9

The surface-enhanced Raman signals of 4-aminothiophenol (4-ATP) attached to the surface of colloidal gold nanoparticles with size distribution of 2 to 5 nm were used as a labeling agent to detect basal cell carcinoma (BCC) of the skin. The enhanced R... Read More about Surface-enhanced Raman spectroscopy study of 4-ATP on gold nanoparticles for basal cell carcinoma fingerprint detection.

Tracing amino acid exchange during host-pathogen interaction by combined stable-isotope time-resolved Raman spectral imaging (2016)
Journal Article
Naemat, A., Elsheikha, H. M., Boiter, R., & Notingher, I. (2016). Tracing amino acid exchange during host-pathogen interaction by combined stable-isotope time-resolved Raman spectral imaging. Scientific Reports, 6, Article 20811. https://doi.org/10.1038/srep20811

This study investigates the temporal and spatial interchange of the aromatic amino acid phenylalanine (Phe) between human retinal pigment epithelial cell line (ARPE-19) and tachyzoites of the apicomplexan protozoan parasite Toxoplasma gondii (T. gond... Read More about Tracing amino acid exchange during host-pathogen interaction by combined stable-isotope time-resolved Raman spectral imaging.

Towards quantitative molecular mapping of cells by Raman microscopy: using AFM for decoupling molecular concentration and cell topography (2015)
Journal Article
Boitor, R., Sinjab, F., Strohbuecker, S., Sottile, V., & Notingher, I. (2016). Towards quantitative molecular mapping of cells by Raman microscopy: using AFM for decoupling molecular concentration and cell topography. Faraday Discussions, 187, https://doi.org/10.1039/C5FD00172B

Raman micro-spectroscopy (RMS) is a non-invasive technique for imaging live cells in-vitro. However, obtaining quantitative molecular information from the Raman spectra is difficult because the intensity of a Raman band is proportional to the number... Read More about Towards quantitative molecular mapping of cells by Raman microscopy: using AFM for decoupling molecular concentration and cell topography.

Applications of Raman micro-spectroscopy to stem cell technology: label-free molecular discrimination and monitoring cell differentiation. (2015)
Journal Article
Ghita, A., Pascut, F. C., Sottile, V., Denning, C., & Notingher, I. (2015). Applications of Raman micro-spectroscopy to stem cell technology: label-free molecular discrimination and monitoring cell differentiation. EPJ Techniques and Instrumentation, 2, Article 6. https://doi.org/10.1140/epjti/s40485-015-0016-8

Stem cell therapy is widely acknowledged as a key medical technology of the 21st century which may provide treatments for many currently incurable diseases. These cells have an enormous potential for cell replacement therapies to cure diseases such a... Read More about Applications of Raman micro-spectroscopy to stem cell technology: label-free molecular discrimination and monitoring cell differentiation..

Analysis of interaction between the apicomplexan protozoan Toxoplasma gondii and host cells using label-free Raman spectroscopy (2014)
Journal Article
Naemat, A., Elsheikha, H. M., Al-sandaqchib, A., Kong, K., Ghita, A., & Notingher, I. (2015). Analysis of interaction between the apicomplexan protozoan Toxoplasma gondii and host cells using label-free Raman spectroscopy. Analyst, 140(3), 756-764. https://doi.org/10.1039/c4an01810a

Label-free imaging using Raman micro-spectroscopy (RMS) was used to characterize the spatio-temporal molecular changes of T. gondii tachyzoites and their host cell microenvironment. Raman spectral maps were recorded from isolated T. gondii tachyzoite... Read More about Analysis of interaction between the apicomplexan protozoan Toxoplasma gondii and host cells using label-free Raman spectroscopy.