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The applied development of a tiered multilocus sequence typing (MLST) scheme for Dichelobacter nodosus (2018)
Journal Article
Blanchard, A. M., Jolley, K. A., Maiden, M. C., Coffey, T. J., Maboni, G., Staley, C. E., …Tötemeyer, S. (in press). The applied development of a tiered multilocus sequence typing (MLST) scheme for Dichelobacter nodosus. Frontiers in Microbiology, 9, Article 551. https://doi.org/10.3389/fmicb.2018.00551

Dichelobacter nodosus (D. nodosus) is the causative pathogen of ovine footrot, a disease that has a significant welfare and financial impact on the global sheep industry. Previous studies into the phylogenetics of D. nodosus have focused on Australia... Read More about The applied development of a tiered multilocus sequence typing (MLST) scheme for Dichelobacter nodosus.

A novel 3D skin explant model to study anaerobic bacterial infection (2017)
Journal Article
Maboni, G., Davenport, R., Sessford, K., Baiker, K., Jensen, T. K., Blanchard, A. M., …Tötemeyer, S. (2017). A novel 3D skin explant model to study anaerobic bacterial infection. Frontiers in Cellular and Infection Microbiology, 7, Article 404. https://doi.org/10.3389/fcimb.2017.00404

Skin infection studies are often limited by financial and ethical constraints, and alternatives, such as monolayer cell culture, do not reflect many cellular processes limiting their application. For a more functional replacement, 3D skin culture mod... Read More about A novel 3D skin explant model to study anaerobic bacterial infection.

A distinct bacterial dysbiosis associated skin inflammation in ovine footrot (2017)
Journal Article
Maboni, G., Blanchard, A. M., Frosth, S., Stewart, C. E., Emes, R. D., & Tötemeyer, S. (in press). A distinct bacterial dysbiosis associated skin inflammation in ovine footrot. Scientific Reports, 7, Article 45220. https://doi.org/10.1038/srep45220

Ovine footrot is a highly prevalent bacterial disease caused by Dichelobacter nodosus and characterised by the separation of the hoof horn from the underlying skin. The role of innate immune molecules and other bacterial communities in the developmen... Read More about A distinct bacterial dysbiosis associated skin inflammation in ovine footrot.

PIMMS (Pragmatic Insertional Mutation Mapping System) laboratory methodology a readily accessible tool for identification of essential genes in Streptococcus (2016)
Journal Article
Blanchard, A. M., Egan, S. A., Emes, R. D., Warry, A., & Leigh, J. A. (2016). PIMMS (Pragmatic Insertional Mutation Mapping System) laboratory methodology a readily accessible tool for identification of essential genes in Streptococcus. Frontiers in Microbiology, 7(1645), https://doi.org/10.3389/fmicb.2016.01645

The Pragmatic Insertional Mutation Mapping (PIMMS) laboratory protocol was developed alongside various bioinformatics packages (Blanchard et al., 2015) to enable detection of essential and conditionally essential genes in Streptococcus and related ba... Read More about PIMMS (Pragmatic Insertional Mutation Mapping System) laboratory methodology a readily accessible tool for identification of essential genes in Streptococcus.

Transposon insertion mapping with PIMMS, Pragmatic Insertional Mutation Mapping System (2015)
Journal Article
Blanchard, A. M., Leigh, J. A., Egan, S. A., & Emes, R. D. (2015). Transposon insertion mapping with PIMMS, Pragmatic Insertional Mutation Mapping System. Frontiers in Genetics, 6, Article e139. https://doi.org/10.3389/fgene.2015.00139

The PIMMS (Pragmatic Insertional Mutation Mapping System) pipeline has beendeveloped for simple conditionally essential genome discovery experiments in bacteria.Capable of using raw sequence data files alongside a FASTA sequence of thereference genom... Read More about Transposon insertion mapping with PIMMS, Pragmatic Insertional Mutation Mapping System.