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Escherichia coli DNA repair helicase Lhr is also a uracil-DNA glycosylase (2023)
Journal Article
Buckley, R. J., Lou-Hing, A., Hanson, K. M., Ahmed, N. R., Cooper, C. D., & Bolt, E. L. (2023). Escherichia coli DNA repair helicase Lhr is also a uracil-DNA glycosylase. Molecular Microbiology, 120(2), 298-306. https://doi.org/10.1111/mmi.15123

DNA glycosylases protect genetic fidelity during DNA replication by removing potentially mutagenic chemically damaged DNA bases. Bacterial Lhr proteins are well-characterized DNA repair helicases that are fused to additional 600–700 amino acids of un... Read More about Escherichia coli DNA repair helicase Lhr is also a uracil-DNA glycosylase.

Escherichia coli DNA repair helicase Lhr is also a uracil‐DNA glycosylase (2023)
Journal Article
Buckley, R. J., Lou‐Hing, A., Hanson, K. M., Ahmed, N. R., Cooper, C. D. O., & Bolt, E. L. (2023). Escherichia coli DNA repair helicase Lhr is also a uracil‐DNA glycosylase. Molecular Microbiology, 120(2), 298-306. https://doi.org/10.1111/mmi.15123

DNA glycosylases protect genetic fidelity during DNA replication by removing potentially mutagenic chemically damaged DNA bases. Bacterial Lhr proteins are well‐characterized DNA repair helicases that are fused to additional 600–700 amino acids of un... Read More about Escherichia coli DNA repair helicase Lhr is also a uracil‐DNA glycosylase.

Mechanistic insights into Lhr helicase function in DNA repair (2020)
Journal Article
Buckley, R., Kramm, K., Cooper, C. D. O., Grohmann, D., & Bolt, E. L. (2020). Mechanistic insights into Lhr helicase function in DNA repair. Biochemical Journal, 477(16), 2935–2947. https://doi.org/10.1042/bcj20200379

The DNA helicase Lhr is present throughout archaea, including in the Asgard and Nanoarchaea, and has homologues in bacteria and eukaryotes. It is thought to function in DNA repair but in a context that is not known. Our data show that archaeal Lhr pr... Read More about Mechanistic insights into Lhr helicase function in DNA repair.

DNA binding and unwinding by Hel308 helicase requires dual functions of a winged helix domain (2017)
Journal Article
Northall, S. J., Buckley, R., Jones, N., Penedo, J. C., Soultanas, P., & Bolt, E. L. (2017). DNA binding and unwinding by Hel308 helicase requires dual functions of a winged helix domain. DNA Repair, 57, https://doi.org/10.1016/j.dnarep.2017.07.005

Hel308 helicases promote genome stability linked to DNA replication in archaea, and have homologues in metazoans. In the crystal structure of archaeal Hel308 bound to a tailed DNA duplex, core helicase domains encircle single-stranded DNA (ssDNA) in... Read More about DNA binding and unwinding by Hel308 helicase requires dual functions of a winged helix domain.