Skip to main content

Research Repository

Advanced Search

All Outputs (15)

Improving carbon monoxide tolerance of Cupriavidus necator H16 through adaptive laboratory evolution (2023)
Journal Article
Wickham-Smith, C., Malys, N., & Winzer, K. (2023). Improving carbon monoxide tolerance of Cupriavidus necator H16 through adaptive laboratory evolution. Frontiers in Bioengineering and Biotechnology, 11, https://doi.org/10.3389/fbioe.2023.1178536

Background: The toxic gas carbon monoxide (CO) is abundantly present in synthesis gas (syngas) and certain industrial waste gases that can serve as feedstocks for the biological production of industrially significant chemicals and fuels. For efficien... Read More about Improving carbon monoxide tolerance of Cupriavidus necator H16 through adaptive laboratory evolution.

Biosensor-informed engineering of Cupriavidus necator H16 for autotrophic D-mannitol production (2022)
Journal Article
Hanko, E. K., Sherlock, G., Minton, N. P., & Malys, N. (2022). Biosensor-informed engineering of Cupriavidus necator H16 for autotrophic D-mannitol production. Metabolic Engineering, 72, 24-34. https://doi.org/10.1016/j.ymben.2022.02.003

Cupriavidus necator H16 is one of the most researched carbon dioxide (CO2)-fixing bacteria. It can store carbon in form of the polymer polyhydroxybutyrate and generate energy by aerobic hydrogen oxidation under lithoautotrophic conditions, making C.... Read More about Biosensor-informed engineering of Cupriavidus necator H16 for autotrophic D-mannitol production.

The pMTL70000 modular, plasmid vector series for strain engineering in Cupriavidus necator H16 (2021)
Journal Article
Ehsaan, M., Baker, J., Kovács, K., Malys, N., & Minton, N. P. (2021). The pMTL70000 modular, plasmid vector series for strain engineering in Cupriavidus necator H16. Journal of Microbiological Methods, 189, Article 106323. https://doi.org/10.1016/j.mimet.2021.106323

Cupriavidus necator H16 can convert CO2 into industrial chemicals and fuels. To facilitate its engineering, we designed, built and tested the pMTL70000 modular plasmids comprising standardised Cupriavidus and E. coli replicons, selectable markers and... Read More about The pMTL70000 modular, plasmid vector series for strain engineering in Cupriavidus necator H16.

Engineering Cupriavidus necator H16 for the autotrophic production of (R)-1,3-butanediol (2021)
Journal Article
Gascoyne, J. L., Bommareddy, R. R., Heeb, S., & Malys, N. (2021). Engineering Cupriavidus necator H16 for the autotrophic production of (R)-1,3-butanediol. Metabolic Engineering, 67, 262-276. https://doi.org/10.1016/j.ymben.2021.06.010

Butanediols are widely used in the synthesis of polymers, specialty chemicals and important chemical intermediates. Optically pure R-form of 1,3-butanediol (1,3-BDO) is required for the synthesis of several industrial compounds and as a key intermedi... Read More about Engineering Cupriavidus necator H16 for the autotrophic production of (R)-1,3-butanediol.

A genome-wide approach for identification and characterisation of metabolite-inducible systems (2020)
Journal Article
Hanko, E. K. R., Paiva, A. C., Jonczyk, M., Abbott, M., Minton, N. P., & Malys, N. (2020). A genome-wide approach for identification and characterisation of metabolite-inducible systems. Nature Communications, 11(1), https://doi.org/10.1038/s41467-020-14941-6

© 2020, The Author(s). Inducible gene expression systems are vital tools for the advancement of synthetic biology. Their application as genetically encoded biosensors has the potential to contribute to diagnostics and to revolutionise the field of mi... Read More about A genome-wide approach for identification and characterisation of metabolite-inducible systems.

Functional genetic elements for controlling gene expression in Cupriavidus necator H16 (2018)
Journal Article
Alagesan, S., Hanko, E. K., Malys, N., Ehsaan, M., Winzer, K., & Minton, N. P. (2018). Functional genetic elements for controlling gene expression in Cupriavidus necator H16. Applied and Environmental Microbiology, https://doi.org/10.1128/AEM.00878-18

A robust and predictable control of gene expression plays an important role in synthetic biology and biotechnology applications. Development and quantitative evaluation of functional genetic elements such as constitutive and inducible promoters, as w... Read More about Functional genetic elements for controlling gene expression in Cupriavidus necator H16.

A transcription factor-based biosensor for detection of itaconic acid (2018)
Journal Article
Hanko, E. K., Minton, N. P., & Malys, N. (2018). A transcription factor-based biosensor for detection of itaconic acid. ACS Synthetic Biology, 7(5), 1436–1446. https://doi.org/10.1021/acssynbio.8b00057

Itaconic acid is an important platform chemical that can easily be incorporated into polymers and has the potential to replace petrochemical-based acrylic or methacrylic acid. A number of microorganisms have been developed for the biosynthesis of ita... Read More about A transcription factor-based biosensor for detection of itaconic acid.

Dispersion of relative importance values contributes to the ranking uncertainty: sensitivity analysis of Multiple Criteria Decision-Making methods (2018)
Journal Article
Maliene, V., Dixon-Gough, R., & Malys, N. (2018). Dispersion of relative importance values contributes to the ranking uncertainty: sensitivity analysis of Multiple Criteria Decision-Making methods. Applied Soft Computing, 67, https://doi.org/10.1016/j.asoc.2018.03.003

Multiple Criteria Decision-Making (MCDM) methods are widely used in research and industrial applications. These methods rely heavily on expert perceptions and are often sensitive to the assumptions made. The reliability and robustness of MCDM analysi... Read More about Dispersion of relative importance values contributes to the ranking uncertainty: sensitivity analysis of Multiple Criteria Decision-Making methods.

13C-assisted metabolic flux analysis to investigate heterotrophic and mixotrophic metabolism in Cupriavidus necator H16 (2017)
Journal Article
Alagesan, S., Minton, N. P., & Malys, N. (2018). 13C-assisted metabolic flux analysis to investigate heterotrophic and mixotrophic metabolism in Cupriavidus necator H16. Metabolomics, 14(9), https://doi.org/10.1007/s11306-017-1302-z

Introduction. Cupriavidus necator H16 is a gram-negative bacterium, capable of lithoautotrophic growth by utilizing hydrogen as an energy source and fixing carbon dioxide (CO2) through Calvin-Benson-Bassham (CBB) cycle. The potential to utilize synth... Read More about 13C-assisted metabolic flux analysis to investigate heterotrophic and mixotrophic metabolism in Cupriavidus necator H16.

Characterisation of a 3-hydroxypropionic acid-inducible system from Pseudomonas putida for orthogonal gene expression control in Escherichia coli and Cupriavidus necator (2017)
Journal Article
Hanko, E. K., Minton, N. P., & Malys, N. (in press). Characterisation of a 3-hydroxypropionic acid-inducible system from Pseudomonas putida for orthogonal gene expression control in Escherichia coli and Cupriavidus necator. Scientific Reports, 7, Article 1724. https://doi.org/10.1038/s41598-017-01850-w

3-hydroxypropionic acid (3-HP) is an important platform chemical used as a precursor for production of added-value compounds such as acrylic acid. Metabolically engineered yeast, Escherichia coli, cyanobacteria and other microorganisms have been deve... Read More about Characterisation of a 3-hydroxypropionic acid-inducible system from Pseudomonas putida for orthogonal gene expression control in Escherichia coli and Cupriavidus necator.

Housing stakeholder preferences for the “soft” features of sustainable and healthy housing design in the UK (2016)
Journal Article
Prochorskaite, A., Couch, C., Malys, N., & Maliene, V. (2016). Housing stakeholder preferences for the “soft” features of sustainable and healthy housing design in the UK. International Journal of Environmental Research and Public Health, 13(1), https://doi.org/10.3390/ijerph13010111

It is widely recognised that the quantity and sustainability of new homes in the UK need to increase. However, it is important that sustainable housing is regarded holistically, and not merely in environmental terms, and incorporates elements that en... Read More about Housing stakeholder preferences for the “soft” features of sustainable and healthy housing design in the UK.

Comparative analysis of MCDM methods for the assessment of sustainable housing affordability (2015)
Journal Article
Mulliner, E., Malys, N., & Maliene, V. (2016). Comparative analysis of MCDM methods for the assessment of sustainable housing affordability. Omega, 59(Part B), https://doi.org/10.1016/j.omega.2015.05.013

While affordability is traditionally assessed in economic terms, this paper tests a new assessment method that draws closer links with sustainability by considering economic, social and environmental criteria that impact on a household’s quality of l... Read More about Comparative analysis of MCDM methods for the assessment of sustainable housing affordability.

Close-range photogrammetry enables documentation of environment-induced deformation of architectural heritage (2015)
Journal Article
Sužiedelytė-Visockienė, J., Bagdžiūnaitė, R., Malys, N., & Maliene, V. (2015). Close-range photogrammetry enables documentation of environment-induced deformation of architectural heritage. Environmental Engineering and Management Journal, 14(6),

Deformation, damage and permanent loss of heritage assets due to various physical and environmental factors has always been a major problem. As the availability of funds for conservation and restoration is limited, the digital documentation of herita... Read More about Close-range photogrammetry enables documentation of environment-induced deformation of architectural heritage.

Symbiotic and antibiotic interactions between gut commensal microbiota and host immune system (2015)
Journal Article
Malys, M. K., Campbell, L., & Malys, N. (2015). Symbiotic and antibiotic interactions between gut commensal microbiota and host immune system. Medicina, 51(2), https://doi.org/10.1016/j.medici.2015.03.001

The human gut commensal microbiota forms a complex population of microorganisms that survive by maintaining a symbiotic relationship with the host. Amongst the metabolic benefits it brings, formation of adaptive immune system and maintenance of its h... Read More about Symbiotic and antibiotic interactions between gut commensal microbiota and host immune system.

Enzyme characterisation and kinetic modelling of the pentose phosphate pathway in yeast (2014)
Journal Article
Messiha, H. L., Kent, E., Malys, N., Carroll, K. M., Swainston, N., Mendes, P., & Smallbone, K. (2014). Enzyme characterisation and kinetic modelling of the pentose phosphate pathway in yeast. PeerJ, 2(e146v4), https://doi.org/10.7287/peerj.preprints.146v4

We present the quantification and kinetic characterisation of the enzymes of the pentose phosphate pathway in Saccharomyces cerevisiae. The data are combined into a mathematical model that describes the dynamics of this system and allows us to predic... Read More about Enzyme characterisation and kinetic modelling of the pentose phosphate pathway in yeast.