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The effects of exogenous fibrolytic enzymes on the in vitro generation of xylooligosaccharides and monosaccharides is dependent upon cereal type (2022)
Journal Article
Dale, T., Bedford, M. R., Tucker, G. A., Brameld, J. M., & Parr, T. (2022). The effects of exogenous fibrolytic enzymes on the in vitro generation of xylooligosaccharides and monosaccharides is dependent upon cereal type. Animal Feed Science and Technology, 292, Article 115406. https://doi.org/10.1016/j.anifeedsci.2022.115406

Fibrolytic enzymes are routinely added to non-ruminant livestock feeds to help degrade the non-starch polysaccharide (NSP) contents and thereby improve feed efficiency. This study investigated the range of xylooligosaccharides (XOS) and monosaccharid... Read More about The effects of exogenous fibrolytic enzymes on the in vitro generation of xylooligosaccharides and monosaccharides is dependent upon cereal type.

Bioactivity and anthocyanin content of microwave-assisted subcritical water extracts of Manipur black rice (Chakhao) bran and straw (2021)
Journal Article
Ramakrishna, P., Moirangthem, K., Amer, M. H., & Tucker, G. A. (2021). Bioactivity and anthocyanin content of microwave-assisted subcritical water extracts of Manipur black rice (Chakhao) bran and straw. Future Foods, 3, Article 100030. https://doi.org/10.1016/j.fufo.2021.100030

Chakhao, the Geographical Indication (GI) tagged aromatic black rice of Manipur, India, is a rich source of bioactive compounds such as anthocyanins. However, there is limited work available on Chakhao and research has focused on its grain. No report... Read More about Bioactivity and anthocyanin content of microwave-assisted subcritical water extracts of Manipur black rice (Chakhao) bran and straw.

Impact of mixed feedstock of wheat straw, willow and Miscanthus on enzymatic hydrolysis and inhibitor production after microwave hydrothermal pre-treatment in Europe (2021)
Journal Article
Moirangthem, K., Greetham, D., Craigon, J., & Tucker, G. (2022). Impact of mixed feedstock of wheat straw, willow and Miscanthus on enzymatic hydrolysis and inhibitor production after microwave hydrothermal pre-treatment in Europe. Biofuels, 13(6), 727-734. https://doi.org/10.1080/17597269.2021.1894782

Lignocellulosic feedstocks for biorefinery are likely to be seasonal and the supply of feedstock to cellulosic biorefineries remains a challenge. One way to overcome this is by utilizing a mixed feedstock which facilitates the maintenance of a year-r... Read More about Impact of mixed feedstock of wheat straw, willow and Miscanthus on enzymatic hydrolysis and inhibitor production after microwave hydrothermal pre-treatment in Europe.

Enzyme digestion of biofiber from mechanical heat treated municipal solid waste: Accessing kinetic and rheological design data using a pilot-scale high-solids mixer (2020)
Journal Article
Ibbett, R., Tucker, G., McKechnie, J., & Meng, F. (2020). Enzyme digestion of biofiber from mechanical heat treated municipal solid waste: Accessing kinetic and rheological design data using a pilot-scale high-solids mixer. Biomass and Bioenergy, 143, Article 105817. https://doi.org/10.1016/j.biombioe.2020.105817

© 2020 Mechanical heat treatment of municipal solid waste offers a route for the production and separation of a biofiber fraction with high organic content, which is a potential source of sugars for biochemical conversion to chemicals and fuels. This... Read More about Enzyme digestion of biofiber from mechanical heat treated municipal solid waste: Accessing kinetic and rheological design data using a pilot-scale high-solids mixer.

The effects of exogenous xylanase supplementation on the in vivo generation of xylooligosaccharides and monosaccharides in broilers fed a wheat-based diet (2020)
Journal Article
Dale, T., Hannay, I., Bedford, M. R., Tucker, G. A., Brameld, J., & Parr, T. (2020). The effects of exogenous xylanase supplementation on the in vivo generation of xylooligosaccharides and monosaccharides in broilers fed a wheat-based diet. British Poultry Science, 61(4), 471-481. https://doi.org/10.1080/00071668.2020.1751805

1. This study aimed to quantify xylanase-induced changes in soluble monosaccharides, xylooligosaccharides (XOS) and volatile fatty acid (VFA) contents of the different sections of the GIT and whether these relate to altered bird performance. 2. An... Read More about The effects of exogenous xylanase supplementation on the in vivo generation of xylooligosaccharides and monosaccharides in broilers fed a wheat-based diet.

Succession of physiological stages hallmarks the transcriptomic response of the fungus Aspergillus niger to lignocellulose (2020)
Journal Article
van Munster, J. M., Daly, P., Blythe, M. J., Ibbett, R., Kokolski, M., Gaddipati, S., …Archer, D. B. (2020). Succession of physiological stages hallmarks the transcriptomic response of the fungus Aspergillus niger to lignocellulose. Biotechnology for Biofuels, 13(1), Article 69. https://doi.org/10.1186/s13068-020-01702-2

Background Understanding how fungi degrade lignocellulose is a cornerstone of improving renewables-based biotechnology, in particular for the production of hydrolytic enzymes. Considerable progress has been made in investigating fungal degradation d... Read More about Succession of physiological stages hallmarks the transcriptomic response of the fungus Aspergillus niger to lignocellulose.

Exploring the Role of Cereal Dietary Fiber in Digestion (2019)
Journal Article
Gouseti, O., Lovegrove, A., Kosik, O., Fryer, P., Mills, C., Gates, F., …Bakalis, S. (2019). Exploring the Role of Cereal Dietary Fiber in Digestion. Journal of Agricultural and Food Chemistry, 67(30), 8419-8424. https://doi.org/10.1021/acs.jafc.9b01847

Increasing the dietary fiber of staple foods such as bread is an attractive way to promote healthy eating in a large part of the population, where dietary fiber consumption is reportedly below the recommended values. However, many consumers prefer wh... Read More about Exploring the Role of Cereal Dietary Fiber in Digestion.

Process simulation and life cycle assessment of converting autoclaved municipal solid waste into butanol and ethanol as transport fuels (2019)
Journal Article
Meng, F., Ibbett, R., de Vrije, T., Metcalf, P., Tucker, G., & McKechnie, J. (2019). Process simulation and life cycle assessment of converting autoclaved municipal solid waste into butanol and ethanol as transport fuels. Waste Management, 89, 177-189. https://doi.org/10.1016/j.wasman.2019.04.003

In 2015/2016, the total municipal solid waste (MSW) collected by local authority in the U.K. was 26 million tonnes and over 57% is still put into landfill or incinerated. MSW is a promising feedstock for bio-butanol production as it has a high lignoc... Read More about Process simulation and life cycle assessment of converting autoclaved municipal solid waste into butanol and ethanol as transport fuels.

The establishment of a marine focused biorefinery for bioethanol production using seawater and a novel marine yeast strain (2018)
Journal Article
Zaky, A. S., Greetham, D., Tucker, G. A., & Du, C. (2018). The establishment of a marine focused biorefinery for bioethanol production using seawater and a novel marine yeast strain. Scientific Reports, 8(1), https://doi.org/10.1038/s41598-018-30660-x

Current technologies for bioethanol production rely on the use of freshwater for preparing the fermentation media and use yeasts of a terrestrial origin. Life cycle assessment has suggested that between 1,388 to 9,812 litres of freshwater are consume... Read More about The establishment of a marine focused biorefinery for bioethanol production using seawater and a novel marine yeast strain.

A proposed biomass char classification system (2018)
Journal Article
Lester, E., Avila, C., Pang, C. H., Williams, O., Perkins, J., Gaddipatti⁠, S., …Wu, T. (2018). A proposed biomass char classification system. Fuel, 232, https://doi.org/10.1016/j.fuel.2018.05.153

A new classification system is proposed for the morphological characterisation of char structures from biomass. These char structures are unlike the coal chars that have an established nomenclature via the International Committee of Coal and Organic... Read More about A proposed biomass char classification system.

Treatment of dragonfruit ( Hylocereus polyrhizus ) with salicylic acid and methyl jasmonate improves postharvest physico-chemical properties and antioxidant activity during cold storage (2017)
Journal Article
Mustafa, M. A., Ali, A., Seymour, G. B., & Tucker, G. A. (2018). Treatment of dragonfruit ( Hylocereus polyrhizus ) with salicylic acid and methyl jasmonate improves postharvest physico-chemical properties and antioxidant activity during cold storage. Scientia Horticulturae, 231, https://doi.org/10.1016/j.scienta.2017.09.041

As the market for tropical fruit constantly expands, cold storage is increasingly used for transporting fruits over long distances. This is an economic postharvest tool, yet challenges tropical fruits by exposure to chilling injury. An assessment of... Read More about Treatment of dragonfruit ( Hylocereus polyrhizus ) with salicylic acid and methyl jasmonate improves postharvest physico-chemical properties and antioxidant activity during cold storage.

Understanding the mechanisms of cooperative physico-chemical treatment and mechanical disintegration of biomass as a route for enhancing enzyme saccharification (2017)
Journal Article
Ibbett, R., Gaddipati, S., & Tucker, G. A. (in press). Understanding the mechanisms of cooperative physico-chemical treatment and mechanical disintegration of biomass as a route for enhancing enzyme saccharification. Biomass Conversion and Biorefinery, https://doi.org/10.1007/s13399-017-0289-z

A novel chemico-kinetic disintegration model has been applied to study the cooperative relationship between physico-chemical treatment and supplementary wet-state milling of biomass, as an efficient process route to achieve high enzyme accessibility.... Read More about Understanding the mechanisms of cooperative physico-chemical treatment and mechanical disintegration of biomass as a route for enhancing enzyme saccharification.

Expression of Aspergillus niger CAZymes is determined by compositional changes in wheat straw generated by hydrothermal or ionic liquid pretreatments (2017)
Journal Article
Daly, P., van Munster, J. M., Blythe, M. J., Ibbett, R., Kokolski, M., Gaddipati, S., …Archer, D. B. (2017). Expression of Aspergillus niger CAZymes is determined by compositional changes in wheat straw generated by hydrothermal or ionic liquid pretreatments. Biotechnology for Biofuels, 10(1), Article 35. https://doi.org/10.1186/s13068-017-0700-9

Background The capacity of fungi, such as Aspergillus niger, to degrade lignocellulose is harnessed in biotechnology to generate biofuels and high-value compounds from renewable feedstocks. Most feedstocks are currently pretreated to increase enzy... Read More about Expression of Aspergillus niger CAZymes is determined by compositional changes in wheat straw generated by hydrothermal or ionic liquid pretreatments.

A new HPLC method for simultaneously measuring chloride, sugars, organic acids and alcohols in food samples (2016)
Journal Article
Zaky, A. S., Pensupa, N., Andrade-Eiroa, Á., Tucker, G. A., & Du, C. (2017). A new HPLC method for simultaneously measuring chloride, sugars, organic acids and alcohols in food samples. Journal of Food Composition and Analysis, 56, https://doi.org/10.1016/j.jfca.2016.12.010

This paper introduces an original, rapid, efficient and reliable HPLC method for the accurate and simultaneous quantification (g/L) of chloride in samples containing sugars, organic acids and alcohols. Separation was achieved using a HI-Plex H column... Read More about A new HPLC method for simultaneously measuring chloride, sugars, organic acids and alcohols in food samples.

A new isolation and evaluation method for marine-derived yeast spp with potential applications in industrial biotechnology (2016)
Journal Article
Zaky, A. S., Greetham, D., Louis, E. J., Tucker, G. A., & Du, C. (2016). A new isolation and evaluation method for marine-derived yeast spp with potential applications in industrial biotechnology. World Journal of Microbiology and Biotechnology, 26(11), https://doi.org/10.4014/jmb.1605.05074

Yeasts that are present in marine environments have evolved to survive hostile environments that are characterized by high exogenous salt content, high concentrations of inhibitory compounds, and low soluble carbon and nitrogen levels. Therefore, yea... Read More about A new isolation and evaluation method for marine-derived yeast spp with potential applications in industrial biotechnology.

Impact of altered cell wall composition on saccharification efficiency in stem tissue of Arabidopsis RABA GTPase-deficient knockout mutants (2015)
Journal Article
Lunn, D., Ibbett, R., Tucker, G. A., & Lycett, G. W. (2015). Impact of altered cell wall composition on saccharification efficiency in stem tissue of Arabidopsis RABA GTPase-deficient knockout mutants. BioEnergy Research, 8(3), https://doi.org/10.1007/s12155-015-9599-9

Use of biomass for second-generation biofuel production is severely hindered by the inherent recalcitrance of the plant cell wall to digestion. Trafficking is crucial for compartmentalisation within the cell. This process is partly regulated by small... Read More about Impact of altered cell wall composition on saccharification efficiency in stem tissue of Arabidopsis RABA GTPase-deficient knockout mutants.

Authentication of processed meat products by peptidomic analysis using rapid ambient mass spectrometry (2015)
Journal Article
Montowska, M., Alexander, M. R., Tucker, G. A., & Barrett, D. A. (2015). Authentication of processed meat products by peptidomic analysis using rapid ambient mass spectrometry. Food Chemistry, 187, https://doi.org/10.1016/j.foodchem.2015.04.078

We present the application of a novel ambient LESA-MS method for the authentication of processed meat products. A set of 25 species and protein-specific heat stable peptide markers has been detected in processed samples manufactured from beef, pork,... Read More about Authentication of processed meat products by peptidomic analysis using rapid ambient mass spectrometry.

Tryptic Digestion Coupled with Ambient Desorption Electrospray Ionization and Liquid Extraction Surface Analysis Mass Spectrometry Enabling Identification of Skeletal Muscle Proteins in Mixtures and Distinguishing between Beef, Pork, Horse, Chicken, and Turkey Meat (2014)
Journal Article
Montowska, M., Rao, W., Alexander, M. R., Tucker, G. A., & Barrett, D. A. (2014). Tryptic Digestion Coupled with Ambient Desorption Electrospray Ionization and Liquid Extraction Surface Analysis Mass Spectrometry Enabling Identification of Skeletal Muscle Proteins in Mixtures and Distinguishing between Beef, Pork, Horse, Chicken, and Turkey Meat. Analytical Chemistry, 86(9), 4479-4487. https://doi.org/10.1021/ac5003432

The use of ambient desorption electrospray ionization (DESI-MS) mass spectrometry and liquid extraction surface analysis mass spectrometry (LESA-MS) is explored for the first time to analyse skeletal muscle proteins obtained from mixture of standard... Read More about Tryptic Digestion Coupled with Ambient Desorption Electrospray Ionization and Liquid Extraction Surface Analysis Mass Spectrometry Enabling Identification of Skeletal Muscle Proteins in Mixtures and Distinguishing between Beef, Pork, Horse, Chicken, and Turkey Meat.

Structural reorganisation of cellulose fibrils in hydrothermally deconstructed lignocellulosic biomass and relationships with enzyme digestibility (2013)
Journal Article
Ibbett, R., Gaddipati, S., Hill, S., & Tucker, G. A. (2013). Structural reorganisation of cellulose fibrils in hydrothermally deconstructed lignocellulosic biomass and relationships with enzyme digestibility. Biotechnology for Biofuels, 6(1), Article 33. https://doi.org/10.1186/1754-6834-6-33

Background: The investigation of structural organisation in lignocellulose materials is important to understand changes in cellulase accessibility and reactivity resulting from hydrothermal deconstruction, to allow development of strategies to maxi... Read More about Structural reorganisation of cellulose fibrils in hydrothermally deconstructed lignocellulosic biomass and relationships with enzyme digestibility.

Uncovering the genome-wide transcriptional responses of the filamentous fungus Aspergillus niger to lignocellulose using RNA sequencing (2012)
Journal Article
Delmas, S., Pullan, S. T., Gaddipati, S., Kokolski, M., Malla, S., Blythe, M. J., …Archer, D. B. (2012). Uncovering the genome-wide transcriptional responses of the filamentous fungus Aspergillus niger to lignocellulose using RNA sequencing. PLoS Genetics, 8(8), Article e1002875. https://doi.org/10.1371/journal.pgen.1002875

A key challenge in the production of second generation biofuels is the conversion of lignocellulosic substrates into fermentable sugars. Enzymes, particularly those from fungi, are a central part of this process, and many have been isolated and chara... Read More about Uncovering the genome-wide transcriptional responses of the filamentous fungus Aspergillus niger to lignocellulose using RNA sequencing.