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Mapping the interaction between eukaryotic initiation factor 4A (eIF4A) and the inhibitor hippuristanol using carbene footprinting and mass spectrometry (2021)
Journal Article
Lloyd, J. R., Hogan, A., Paschalis, V., Bellamy-Carter, J., Bottley, A., Seymour, G. B., …Oldham, N. J. (2021). Mapping the interaction between eukaryotic initiation factor 4A (eIF4A) and the inhibitor hippuristanol using carbene footprinting and mass spectrometry. Proteomics, 21(21-22), Article 2000288. https://doi.org/10.1002/pmic.202000288

Protein-ligand interactions are central to protein activity and cell functionality. Improved knowledge of these relationships greatly benefits our understanding of key biological processes and aids in rational drug design towards the treatment of cli... Read More about Mapping the interaction between eukaryotic initiation factor 4A (eIF4A) and the inhibitor hippuristanol using carbene footprinting and mass spectrometry.

Characterization of CRISPR mutants targeting genes modulating pectin degradation in ripening tomato (2018)
Journal Article
Wang, D., Samsulrizal, N., Yan, C., Allcock, N. S., Craigon, J., Blanco-Ulate, B., …Seymour, G. B. (2019). Characterization of CRISPR mutants targeting genes modulating pectin degradation in ripening tomato. Plant Physiology, 179(2), 544-557. https://doi.org/10.1104/pp.18.01187

Tomato (Solanum lycopersicum) is a globally important crop with an economic value in the tens of billions of dollars, and a significant supplier of essential vitamins, minerals and phytochemicals in the human diet. Shelf life is a key quality trait r... Read More about Characterization of CRISPR mutants targeting genes modulating pectin degradation in ripening tomato.

Fruit softening: revisiting the role of pectin (2018)
Journal Article
Wang, D., Yeats, T. H., Uluisik, S., Rose, J. K., & Seymour, G. B. (2018). Fruit softening: revisiting the role of pectin. Trends in Plant Science, 23(4), 302-310. https://doi.org/10.1016/j.tplants.2018.01.006

Fruit softening is a major determinant of shelf life and commercial value. Here, we highlight recent work that revisits the role of pectin in fruit softening and primary cell wall structure. These studies demonstrate the importance of pectin and the... Read More about Fruit softening: revisiting the role of pectin.

Treatment of dragonfruit ( Hylocereus polyrhizus ) with salicylic acid and methyl jasmonate improves postharvest physico-chemical properties and antioxidant activity during cold storage (2017)
Journal Article
Mustafa, M. A., Ali, A., Seymour, G. B., & Tucker, G. A. (2018). Treatment of dragonfruit ( Hylocereus polyrhizus ) with salicylic acid and methyl jasmonate improves postharvest physico-chemical properties and antioxidant activity during cold storage. Scientia Horticulturae, 231, https://doi.org/10.1016/j.scienta.2017.09.041

As the market for tropical fruit constantly expands, cold storage is increasingly used for transporting fruits over long distances. This is an economic postharvest tool, yet challenges tropical fruits by exposure to chilling injury. An assessment of... Read More about Treatment of dragonfruit ( Hylocereus polyrhizus ) with salicylic acid and methyl jasmonate improves postharvest physico-chemical properties and antioxidant activity during cold storage.

Methylation profiling RIN3 and MEF2C identifies epigenetic marks associated with sporadic early onset Alzheimer’s disease (2017)
Journal Article
Boden, K. A., Barber, I. S., Clement, N., Patel, T., Guetta-Baranes, T., Brookes, K., …Bottley, A. (2017). Methylation profiling RIN3 and MEF2C identifies epigenetic marks associated with sporadic early onset Alzheimer’s disease. Journal of Alzheimer's Disease Reports, 1(1), https://doi.org/10.3233/ADR-170015

A number of genetic loci associate with early onset Alzheimer’s disease (EOAD), however the drivers of this disease remains enigmatic. Genome wide association and in-vivo modelling have shown that loss-of-function e.g. ABCA7, reduced levels of SIRT1,... Read More about Methylation profiling RIN3 and MEF2C identifies epigenetic marks associated with sporadic early onset Alzheimer’s disease.

The effect of adenosine monophosphate deaminase overexpression on the accumulation of umami-related metabolites in tomatoes (2016)
Journal Article
Chew, B. L., Fisk, I. D., Fray, R., Tucker, G. A., Bodi, Z., Ferguson, A., …Seymour, G. B. (in press). The effect of adenosine monophosphate deaminase overexpression on the accumulation of umami-related metabolites in tomatoes. Plant Cell Reports, 36(1), https://doi.org/10.1007/s00299-016-2058-z

Taste is perceived as one of a combination of five sensations, sweet, sour, bitter, salty, and umami. The umami taste is best known as a savoury sensation and plays a central role in food flavour, palatability, and eating satisfaction. Umami flavour... Read More about The effect of adenosine monophosphate deaminase overexpression on the accumulation of umami-related metabolites in tomatoes.

Genetic improvement of tomato by targeted control of fruit softening (2016)
Journal Article
Uluisik, S., Chapman, N. H., Smith, R., Poole, M., Adams, G., Gillis, R. B., …Seymour, G. B. (2016). Genetic improvement of tomato by targeted control of fruit softening. Nature Biotechnology, 34, 950-952. https://doi.org/10.1038/nbt.3602

Controlling the rate of softening to extend shelf life was a key target for researchers engineering genetically modified (GM) tomatoes in the 1990s, but only modest improvements were achieved. Hybrids grown nowadays contain 'non-ripening mutations' t... Read More about Genetic improvement of tomato by targeted control of fruit softening.

Accessing low-oxidation state taxanes: is taxadiene-4(5)-epoxide on the taxol biosynthetic pathway? (2016)
Journal Article
Barton, N. A., Marsh, B. J., Lewis, W., Narraidoo, N., Seymour, G. B., Fray, R. G., & Hayes, C. J. (in press). Accessing low-oxidation state taxanes: is taxadiene-4(5)-epoxide on the taxol biosynthetic pathway?. Chemical Science, 7(5), https://doi.org/10.1039/c5sc03463a

We have shown for the first time that taxadiene (3) can be epoxidised in a regio- and diastereoselective manner to provide taxadiene-4(5)-epoxide (12) as a single diastereoisomer, and that this epoxide can be rearranged to give taxa-4(20),11(12)-dien... Read More about Accessing low-oxidation state taxanes: is taxadiene-4(5)-epoxide on the taxol biosynthetic pathway?.

A DEMETER-like DNA demethylase protein governs tomato fruit ripening (2015)
Journal Article
Liu, R. E., How-Kit, A., Stammitti, L., Teyssier, E., Rolin, D., Bertrand, A., …Gallusci, P. (2015). A DEMETER-like DNA demethylase protein governs tomato fruit ripening. Proceedings of the National Academy of Sciences, 112(34), https://doi.org/10.1073/pnas.1503362112

In plants, genomic DNA methylation which contributes to development and stress responses can be actively removed by DEMETER-like DNA demethylases (DML). Indeed, in Arabidopsis DMLs are important for maternal imprinting and endosperm demethylation, bu... Read More about A DEMETER-like DNA demethylase protein governs tomato fruit ripening.

Requirement of CHROMOMETHYLASE3 for somatic inheritance of the spontaneous tomato epimutation Colourless non-ripening (2015)
Journal Article
Chen, W., Kong, J., Qin, C., Yu, S., Tan, J., Chen, Y., …Hong, Y. (2015). Requirement of CHROMOMETHYLASE3 for somatic inheritance of the spontaneous tomato epimutation Colourless non-ripening. Scientific Reports, 5, Article 9192. https://doi.org/10.1038/srep09192

Naturally-occurring epimutants are rare and have mainly been described in plants. However how these mutants maintain their epigenetic marks and how they are inherited remain unknown. Here we report that CHROMOMETHYLASE3 (SlCMT3) and other methyltrans... Read More about Requirement of CHROMOMETHYLASE3 for somatic inheritance of the spontaneous tomato epimutation Colourless non-ripening.

A genome-wide metabolomic resource for tomato fruit from Solanum pennellii (2014)
Journal Article
Perez-Fons, L., Wells, T., Corol, D. I., Ward, J. L., Gerrish, C., Beale, M. H., …Fraser, P. D. (in press). A genome-wide metabolomic resource for tomato fruit from Solanum pennellii. Scientific Reports, 4, Article 3859. https://doi.org/10.1038/srep03859

Tomato and its processed products are one of the most widely consumed fruits. Its domestication, however, has resulted in the loss of some 95% of the genetic and chemical diversity of wild relatives. In order to elucidate this diversity, exploit it... Read More about A genome-wide metabolomic resource for tomato fruit from Solanum pennellii.

Analysis of ripening-related gene expression in papaya using an Arabidopsis-based microarray (2012)
Journal Article
Fabi, J. P., Seymour, G. B., Graham, N. S., Broadley, M. R., May, S., Lajolo, F. M., …Oliveira do Nascimento, J. R. (in press). Analysis of ripening-related gene expression in papaya using an Arabidopsis-based microarray. BMC Plant Biology, 12, Article 242. https://doi.org/10.1186/1471-2229-12-242

Background Papaya (Carica papaya L.) is a commercially important crop that produces climacteric fruits with a soft and sweet pulp that contain a wide range of health promoting phytochemicals. Despite its importance, little is known about transcrip... Read More about Analysis of ripening-related gene expression in papaya using an Arabidopsis-based microarray.