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Comprehensive comparison of the interaction of the E2 master regulator with its cognate target DNA sites in 73 human papillomavirus types by sequence statistics (2007)
Journal Article
Sánchez, I. E., Dellarole, M., Gaston, K., & de Prat Gay, G. (2008). Comprehensive comparison of the interaction of the E2 master regulator with its cognate target DNA sites in 73 human papillomavirus types by sequence statistics. Nucleic Acids Research, 36(3), 756-69. doi:10.1093/nar/gkm1104

Mucosal human papillomaviruses (HPVs) are etiological agents of oral, anal and genital cancer. Properties of high- and low-risk HPV types cannot be reduced to discrete molecular traits. The E2 protein regulates viral replication and transcription thr... Read More

A cancer cell-specific inducer of apoptosis (2007)
Journal Article
Green, K. L., Brown, C., Roeder, G. E., Southgate, T. D., & Gaston, K. (2007). A cancer cell-specific inducer of apoptosis. Human Gene Therapy, 18(6), 547-561. doi:10.1089/hum.2006.042

Human papillomavirus (HPV) DNA is found in virtually all cervical cancers, strongly suggesting that these viruses are necessary to initiate this disease. The HPV E2 protein is required for viral replication, but E2 expression is usually lost in HPV-t... Read More

Characterization of an enhancer region of the galanin gene that directs expression to the dorsal root ganglion and confers responsiveness to axotomy (2007)
Journal Article
Bacon, A., Kerr, N. C., Holmes, F. E., Gaston, K., & Wynick, D. (2007). Characterization of an enhancer region of the galanin gene that directs expression to the dorsal root ganglion and confers responsiveness to axotomy. Journal of Neuroscience, 27(24), 6573-6580. doi:10.1523/JNEUROSCI.1596-07.2007

Galanin expression markedly increases in the dorsal root ganglion (DRG) after sciatic nerve axotomy and modulates pain behavior and regeneration of sensory neurons. Here, we describe transgenic mice expressing constructs with varying amounts of seque... Read More

A quadrivalent vaccine for human papillomavirus (2006)
Journal Article
Brown, C., & Gaston, K. (2006). A quadrivalent vaccine for human papillomavirus. Drugs of today (Barcelona, Spain : 1998), 42(11), 703-709. doi:10.1358/dot.2006.42.11.1032056

Cervical cancer is responsible for at least 13,500 deaths a year in the European Union and more than 30 times that worldwide. DNA from a group of human papillomaviruses (HPVs) is found in virtually all cervical carcinoma cells, and these so-called hi... Read More

The recognition of local DNA conformation by the human papillomavirus type 6 E2 protein (2006)
Journal Article
Hooley, E., Fairweather, V., Clarke, A. R., Gaston, K., & Brady, R. L. (2006). The recognition of local DNA conformation by the human papillomavirus type 6 E2 protein. Nucleic Acids Research, 34(14), 3897-3908. doi:10.1093/nar/gkl466

The E2 proteins are transcription/replication factors from papillomaviruses. Human papillomaviruses (HPVs) can be broadly divided in two groups; low-risk HPV subtypes cause benign warts while high-risk HPVs give rise to cervical cancer. Although a ra... Read More

Oligomerisation of the developmental regulator proline rich homeodomain (PRH/Hex) is mediated by a novel proline-rich dimerisation domain (2006)
Journal Article
Soufi, A., Smith, C., Clarke, A. R., Gaston, K., & Jayaraman, P. (2006). Oligomerisation of the developmental regulator proline rich homeodomain (PRH/Hex) is mediated by a novel proline-rich dimerisation domain. Journal of Molecular Biology, 358(4), 943-962. doi:10.1016/j.jmb.2006.02.020

Homeodomain proteins regulate multiple developmental pathways by altering gene expression temporally and in a tissue-specific fashion. The Proline Rich Homeodomain protein (PRH/Hex) is a transcription factor and an essential regulator of embryonic de... Read More

Purification and characterisation of the PRH homeodomain: removal of the N-terminal domain of PRH increases the PRH homeodomain-DNA interaction (2006)
Journal Article
Soufi, A., Gaston, K., & Jayaraman, P. (2006). Purification and characterisation of the PRH homeodomain: removal of the N-terminal domain of PRH increases the PRH homeodomain-DNA interaction. International Journal of Biological Macromolecules, 39(1-3), 45-50. doi:10.1016/j.ijbiomac.2006.01.004

The Proline-Rich Homeodomain (PRH) protein is a regulator of transcription and translation and plays a key role in the control of cell proliferation and cell differentiation. PRH contains an N-terminal proline-rich domain that can repress transcripti... Read More

Diffusible VP22-E2 protein kills bystander cells and offers a route for cervical cancer gene therapy (2006)
Journal Article
Green, K. L., Southgate, T. D., Mulryan, K., Fairbairn, L. J., Stern, P. L., & Gaston, K. (2006). Diffusible VP22-E2 protein kills bystander cells and offers a route for cervical cancer gene therapy. Human Gene Therapy, 17(2), 147-157. doi:10.1089/hum.2006.17.147

Human papillomaviruses (HPVs) are a causative agent of cervical cancer and are implicated in several other types of malignant disease including cancer of the vulva, oral cancer, and skin cancer. In HPV-transformed cells, expression of the viral E6 an... Read More

Measuring the induction or inhibition of apoptosis by HPV proteins (2005)
Book Chapter
Kowalczyk, A. M., Roeder, G. E., Green, K., Stephens, D. J., & Gaston, K. (2005). Measuring the induction or inhibition of apoptosis by HPV proteins. In C. Davy, & J. Doorbar (Eds.), Human Papillomaviruses: methods and protocols, 419 -- 432. Humana Press. doi:10.1385/1-59259-982-6:419

Many viral proteins influence the cellular pathways that control cell proliferation and cell death. Some viral proteins trigger apoptotic cell death, and this may be important in host defense and viral spread. In other cases, viral proteins inhibit a... Read More

Herpes simplex virus VP22-human papillomavirus E2 fusion proteins produced in mammalian or bacterial cells enter mammalian cells and induce apoptotic cell death (2004)
Journal Article
Roeder, G. E., Parish, J. L., Stern, P. L., & Gaston, K. (2004). Herpes simplex virus VP22-human papillomavirus E2 fusion proteins produced in mammalian or bacterial cells enter mammalian cells and induce apoptotic cell death. Biotechnology and Applied Biochemistry, 40(2), 157-165. doi:10.1042/BA20030172

Infection by high‐risk HPV (human papillomavirus) is supposed to be the primary cause of cervical cancer. The HPV E2 protein (E2) is a DNA‐binding protein that regulates viral gene expression and is required for efficient viral replication. Overexpre... Read More

Comparison of the structure and DNA-binding properties of the E2 proteins from an oncogenic and a non-oncogenic human papillomavirus (2003)
Journal Article
Dell, G., Wilkinson, K. W., Tranter, R., Parish, J., Brady, R. L., & Gaston, K. (2003). Comparison of the structure and DNA-binding properties of the E2 proteins from an oncogenic and a non-oncogenic human papillomavirus. Journal of Molecular Biology, 334(5), 979-991. doi:10.1016/j.jmb.2003.10.009

Human papillomaviruses (HPVs) that infect the genital tract can be divided into two groups: high-risk HPV types, such as HPV 16 and HPV 18, are associated with cancer, low-risk HPV types, such as HPV 6, are associated with benign warts. In both high-... Read More

Human papillomavirus type 16 E2- and L1-specific serological and T-cell responses in women with vulva intraepithelial neoplasia (2003)
Journal Article
Davidson, E. J., Sehr, P., Faulkner, R. L., Parish, J. L., Gaston, K., Moore, R. A., …Stern, P. L. (2003). Human papillomavirus type 16 E2- and L1-specific serological and T-cell responses in women with vulva intraepithelial neoplasia. Journal of General Virology, 84(8), 2089-2097. doi:10.1099/vir.0.19095-0

Human papillomavirus type 16 (HPV-16)-associated vulval intraepithelial neoplasia (VIN) is frequently a chronic, multifocal high-grade condition with an appreciable risk of progression to vulval cancer. The requirement to treat women with VIN has... Read More

The transcriptional repressor protein PRH interacts with the proteasome (2003)
Journal Article
Bess, K. L., Swingler, T. E., Rivett, A. J., Gaston, K., & Jayaraman, P. (2003). The transcriptional repressor protein PRH interacts with the proteasome. Biochemical Journal, 374(3), 667-675. doi:10.1042/BJ20030769

PRH (proline-rich homeodomain protein)/Hex is important in the control of cell proliferation and differentiation. We have shown previously that PRH contains two domains that can bring about transcriptional repression independently; the PRH homeodomai... Read More

Transcriptional repression in eukaryotes: repressors and repression mechanisms (2003)
Journal Article
Gaston, K., & Jayaraman, P. (2003). Transcriptional repression in eukaryotes: repressors and repression mechanisms. Cellular and Molecular Life Sciences, 60(4), 721-741. doi:10.1007/s00018-003-2260-3

For many, if not most genes, the initiation of transcription is the principle point at which their expression is regulated. Transcription factors, some of which bind to specific DNA sequences, generally either activate or repress promoter activity an... Read More

Purification of the proline-rich homeodomain protein (2002)
Journal Article
Butcher, A. J., Gaston, K., & Jayaraman, P. (2003). Purification of the proline-rich homeodomain protein. Journal of Chromatography B, 786(1-2), 3-6. doi:10.1016/S1570-0232(02)00740-7

The proline-rich homeodomain protein (PRH), also known as Hex, is a transcriptional repressor expressed in a variety of cell types. The PRH protein contains a proline-rich N-terminal domain that can repress transcription when attached to a heterologo... Read More

The interleukin-10 – 1082 G/A polymorphism: allele frequency in different populations and functional significance (2002)
Journal Article
Rees, L., Wood, N., Gillespie, K., Lai, K., Gaston, K., & Mathieson, P. (2002). The interleukin-10 – 1082 G/A polymorphism: allele frequency in different populations and functional significance. Cellular and Molecular Life Sciences, 59(3), 560-569. doi:10.1007/s00018-002-8448-0

Genotypic variation in the human interleukin-10 (IL-10) promoter may account for marked inter-individual variation in IL-10 production and may influence susceptibility to autoimmune diseases. The G/A polymorphism at position -1082 has been linked to... Read More

Combination of progesterone and mifepristone for cancer therapy (2001)
Other
Gaston, K. (2001). Combination of progesterone and mifepristone for cancer therapy

Use of a steroid hormone or steroid hormone analogue and at least a portioUse of a steroid hormone or steroid hormone analogue and at least a portion of HPV E2 protein in the preparation of a pharmaceutical composition for the treatment of cervical c... Read More

Human T-cell responses to HPV 16 E2 generated with monocyte-derived dendritic cells (2001)
Journal Article
Davidson, E. J., Brown, M. D., Burt, D. J., Parish, J. L., Gaston, K., Kitchener, H. C., …Stern, P. L. (2001). Human T-cell responses to HPV 16 E2 generated with monocyte-derived dendritic cells. International Journal of Cancer, 94(6), 807-812. https://doi.org/10.1002/ijc.1558

Persistent infection with human papillomavirus (HPV) type 16 has been implicated in the etiology of cervical cancer. The E2 protein is required early in viral infection and therefore may serve as a useful immune target for a vaccine aimed at preventi... Read More