All Outputs (43)

Use of Magnetic Resonance Imaging for Visualization of Oral Dosage Forms in the Human Stomach: A Scoping Review (2024)
Journal Article
Akbar, T., Gershkovich, P., Stamatopoulos, K., Gowland, P. A., Stolnik, S., Butler, J., & Marciani, L. (2024). Use of Magnetic Resonance Imaging for Visualization of Oral Dosage Forms in the Human Stomach: A Scoping Review. Molecular Pharmaceutics, https://doi.org/10.1021/acs.molpharmaceut.3c01123

Oral dosage forms are the most widely and frequently used formulations to deliver active pharmaceutical ingredients (APIs), due to their ease of administration and noninvasiveness. Knowledge of intragastric release rates and gastric mixing is crucial... Read More about Use of Magnetic Resonance Imaging for Visualization of Oral Dosage Forms in the Human Stomach: A Scoping Review.

Synthetic macromolecular peptide-mimetics with amino acid substructure residues as protein stabilising excipients (2023)
Journal Article
Foralosso, R., Kopiasz, R. J., Mantovani, G., Stolnik, S., & Alexander, C. (2024). Synthetic macromolecular peptide-mimetics with amino acid substructure residues as protein stabilising excipients. Journal of Materials Chemistry B, 12, 1022-1030. https://doi.org/10.1039/d3tb02102e

The clinical use of protein and peptide biotherapeutics requires fabrication of stable products. This particularly concerns stability towards aggregation of proteins or peptides. Here, we tested a hypothesis that interactions between a synthetic pept... Read More about Synthetic macromolecular peptide-mimetics with amino acid substructure residues as protein stabilising excipients.

Identifying new biomarkers of aggressive Group 3 and SHH medulloblastoma using 3D hydrogel models, single cell RNA sequencing and 3D OrbiSIMS imaging (2023)
Journal Article
Linke, F., Johnson, J. E. C., Kern, S., Bennett, C. D., Lourdusamy, A., Lea, D., …Coyle, B. (2023). Identifying new biomarkers of aggressive Group 3 and SHH medulloblastoma using 3D hydrogel models, single cell RNA sequencing and 3D OrbiSIMS imaging. Acta Neuropathologica Communications, 11(1), Article 6. https://doi.org/10.1186/s40478-022-01496-4

The most common malignant brain tumour in children, medulloblastoma (MB), is subdivided into four clinically relevant molecular subgroups, although targeted therapy options informed by understanding of different cellular features are lacking. Here, b... Read More about Identifying new biomarkers of aggressive Group 3 and SHH medulloblastoma using 3D hydrogel models, single cell RNA sequencing and 3D OrbiSIMS imaging.

Direct routes to functional RAFT agents from substituted N-alkyl maleimides (2022)
Journal Article
Catania, R., Foralosso, R., Spanos, L., Russo, E., Mastrotto, F., Gurnani, P., …Mantovani, G. (2022). Direct routes to functional RAFT agents from substituted N-alkyl maleimides. Polymer Chemistry, 13(45), 6261-6267. https://doi.org/10.1039/d1py01565f

N-substituted maleimides have become an indispensable tool for the synthesis of bioconjugates and functional materials. Herein, we present three strategies for the incorporation of N-alkyl substituted maleimides into RAFT agents and show that these m... Read More about Direct routes to functional RAFT agents from substituted N-alkyl maleimides.

A mechanoresponsive nano-sized carrier achieves intracellular release of drug on external ultrasound stimulus (2022)
Journal Article
Catania, R., Onion, D., Russo, E., Zelzer, M., Mantovani, G., Huett, A., & Stolnik, S. (2022). A mechanoresponsive nano-sized carrier achieves intracellular release of drug on external ultrasound stimulus. RSC Advances, 12(26), 16561-16569. https://doi.org/10.1039/d2ra02307e

Control over intracellular release of therapeutic compounds incorporated into nano-carriers will open new possibilities for targeted treatments of various diseases including cancer, and viral and bacterial infections. Here we report our study on mech... Read More about A mechanoresponsive nano-sized carrier achieves intracellular release of drug on external ultrasound stimulus.

Enhanced permeation by amphiphilic surfactant is spatially heterogenous at membrane and cell level (2022)
Journal Article
Cavanagh, R., Shubber, S., Vllasaliu, D., & Stolnik, S. (2022). Enhanced permeation by amphiphilic surfactant is spatially heterogenous at membrane and cell level. Journal of Controlled Release, 345, 734-743. https://doi.org/10.1016/j.jconrel.2022.03.053

In the context of increased interest in permeability enhancement technologies to achieve mucosal delivery of drugs and biologics, we report our study on effects of the amphiphilic surfactant at cell membrane and cell population levels. Our results sh... Read More about Enhanced permeation by amphiphilic surfactant is spatially heterogenous at membrane and cell level.

Application of In Vivo MRI Imaging to Track a Coated Capsule and Its Disintegration in the Gastrointestinal Tract in Human Volunteers (2022)
Journal Article
Sulaiman, S., Gershkovich, P., Hoad, C. L., Calladine, M., Spiller, R. C., Stolnik, S., & Marciani, L. (2022). Application of In Vivo MRI Imaging to Track a Coated Capsule and Its Disintegration in the Gastrointestinal Tract in Human Volunteers. Pharmaceutics, 14(2), Article 270. https://doi.org/10.3390/pharmaceutics14020270

Oral specially coated formulations have the potential to improve treatment outcomes of a range of diseases in distal intestinal tract whilst limiting systemic drug absorption and adverse effects. Their development is challenging, partly because of li... Read More about Application of In Vivo MRI Imaging to Track a Coated Capsule and Its Disintegration in the Gastrointestinal Tract in Human Volunteers.

Investigating histidinylated highly branched poly(lysine) for siRNA delivery (2021)
Journal Article
Alazzo, A., Gumus, N., Gurnani, P., Stolnik, S., Rahman, R., Spriggs, K., & Alexander, C. (2022). Investigating histidinylated highly branched poly(lysine) for siRNA delivery. Journal of Materials Chemistry B, 10(2), 236-246. https://doi.org/10.1039/d1tb01793d

The temporary silencing of disease-associated genes utilising short interfering RNA (siRNA) is a potent and selective route for addressing a wide range of life limiting disorders. However, the few clinically approved siRNA therapies rely on lipid bas... Read More about Investigating histidinylated highly branched poly(lysine) for siRNA delivery.

Assessing lymphatic uptake of lipids using magnetic resonance imaging: A feasibility study in healthy human volunteers with potential application for tracking lymph node delivery of drugs and formulation excipients (2021)
Journal Article
Jewell, A., Williams, H., Hoad, C. L., Gellert, P. R., Ashford, M. B., Butler, J., …Gershkovich, P. (2021). Assessing lymphatic uptake of lipids using magnetic resonance imaging: A feasibility study in healthy human volunteers with potential application for tracking lymph node delivery of drugs and formulation excipients. Pharmaceutics, 13(9), Article 1343. https://doi.org/10.3390/pharmaceutics13091343

Dietary lipids and some pharmaceutical lipid excipients can facilitate the targeted delivery of drugs to the intestinal lymphatics. Here, the feasibility of magnetic resonance imaging (MRI) for imaging lipid uptake into the intestinal lymphatics was... Read More about Assessing lymphatic uptake of lipids using magnetic resonance imaging: A feasibility study in healthy human volunteers with potential application for tracking lymph node delivery of drugs and formulation excipients.

Multi-component bioresponsive nanoparticles for synchronous delivery of docetaxel and TUBB3 siRNA to lung cancer cells (2021)
Journal Article
Conte, C., Monteiro, P., Gurnani, P., Stolnik, S., Ungaro, F., Quaglia, F., …Alexander, C. (2021). Multi-component bioresponsive nanoparticles for synchronous delivery of docetaxel and TUBB3 siRNA to lung cancer cells. Nanoscale, 13(26), 11414-11426. https://doi.org/10.1039/d1nr02179f

Bioresponsive nanoparticles (NPs) are of interest for anticancer nanomedicines, owing to the possibility to ‘design in’ selective modulation of drug release at target sites. Here we describe the double emulsion formulation of redox-responsive NPs bas... Read More about Multi-component bioresponsive nanoparticles for synchronous delivery of docetaxel and TUBB3 siRNA to lung cancer cells.

Structural and binding characterization of the LacdiNAc-specific adhesin (LabA; HopD) exodomain from Helicobacter pylori (2020)
Journal Article
Paraskevopoulou, V., Schimpl, M., Overman, R. C., Stolnik, S., Chen, Y., Nguyen, L., …Falcone, F. H. (2021). Structural and binding characterization of the LacdiNAc-specific adhesin (LabA; HopD) exodomain from Helicobacter pylori. Current Research in Structural Biology, 3, 19-29. https://doi.org/10.1016/j.crstbi.2020.12.004

Helicobacter pylori (H. pylori) uses several outer membrane proteins for adhering to its host's gastric mucosa, an important step in establishing and preserving colonization. Several adhesins (SabA, BabA, HopQ) have been characterized in terms of the... Read More about Structural and binding characterization of the LacdiNAc-specific adhesin (LabA; HopD) exodomain from Helicobacter pylori.

3D hydrogels reveal medulloblastoma subgroup differences and identify extracellular matrix subtypes that predict patient outcome (2020)
Journal Article
Linke, F., Aldighieri, M., Lourdusamy, A., Grabowska, A. M., Stolnik, S., Kerr, I. D., …Coyle, B. (2021). 3D hydrogels reveal medulloblastoma subgroup differences and identify extracellular matrix subtypes that predict patient outcome. Journal of Pathology, 253(3), 326-338. https://doi.org/10.1002/path.5591

© 2020 The Authors. The Journal of Pathology published by John Wiley & Sons, Ltd. on behalf of The Pathological Society of Great Britain and Ireland. Medulloblastoma (MB) is the most common malignant brain tumour in children and is subdivided into... Read More about 3D hydrogels reveal medulloblastoma subgroup differences and identify extracellular matrix subtypes that predict patient outcome.

Development of an in vitro system to study the interactions of aerosolized drugs with pulmonary mucus (2020)
Journal Article
Alqahtani, S., Roberts, C. J., Stolnik, S., & Bosquillon, C. (2020). Development of an in vitro system to study the interactions of aerosolized drugs with pulmonary mucus. Pharmaceutics, 12(2), https://doi.org/10.3390/pharmaceutics12020145

© 2020 by the authors. Licensee MDPI, Basel, Switzerland. Mucus is the first biological component inhaled drugs encounter on their journey towards their pharmacological target in the upper airways. Yet, how mucus may influence drug disposition and ef... Read More about Development of an in vitro system to study the interactions of aerosolized drugs with pulmonary mucus.

A simple and efficient method for polymer coating of iron oxide nanoparticles (2019)
Journal Article
Abushrida, A., Elhuni, I., Taresco, V., Marciani, L., Stolnik, S., & Garnett, M. C. (2020). A simple and efficient method for polymer coating of iron oxide nanoparticles. Journal of Drug Delivery Science and Technology, 55, https://doi.org/10.1016/j.jddst.2019.101460

Iron oxide nanoparticles (IONP) have many possible uses including as MRI contrast agents, for drug delivery and cell labelling, but need to be stabilised and to be rendered biocompatible through appropriate coatings. Many current coatings are subopti... Read More about A simple and efficient method for polymer coating of iron oxide nanoparticles.

Targeted PEG-poly(glutamic acid) complexes for inhalation protein delivery to the lung (2019)
Journal Article
Nieto-Orellana, A., Li, H., Rosiere, R., Wauthoz, N., Williams, H., Monteiro, C., …Stolnik, S. (2019). Targeted PEG-poly(glutamic acid) complexes for inhalation protein delivery to the lung. Journal of Controlled Release, 316, 250-262. https://doi.org/10.1016/j.jconrel.2019.10.012

Pulmonary delivery is increasingly seen as an attractive, non-invasive route for the delivery of forthcoming protein therapeutics. In this context, here we describe protein complexes with a new ‘complexing excipient’ - vitamin B12-targeted poly(ethyl... Read More about Targeted PEG-poly(glutamic acid) complexes for inhalation protein delivery to the lung.

Poly(triazolyl methacrylate) glycopolymers as potential targeted unimolecular nanocarriers (2019)
Journal Article
Madeira Do O, J., Foralosso, R., Yilmaz, G., Mastrotto, F., King, P. J. S., Xerri, R. M., …Mantovani, G. (2019). Poly(triazolyl methacrylate) glycopolymers as potential targeted unimolecular nanocarriers. Nanoscale, 11(44), 21155-21166. https://doi.org/10.1039/C9NR05836B

Synthetic glycopolymers are increasingly investigated as multivalent ligands for a range of biological and biomedical applications. This study indicates that glycopolymers with fine-tuned balance between hydrophilic sugar pendant units and relatively... Read More about Poly(triazolyl methacrylate) glycopolymers as potential targeted unimolecular nanocarriers.

Introduction of a C-terminal hexa-lysine tag increases thermal stability of the LacDiNac binding adhesin (LabA) exodomain from Helicobacter pylori (2019)
Journal Article
Paraskevopoulou, V., Artiaga, V. G., Rowlinson, R., Winkler, G. S., Gellert, P., Stolnik, S., …Falcone, F. H. (2019). Introduction of a C-terminal hexa-lysine tag increases thermal stability of the LacDiNac binding adhesin (LabA) exodomain from Helicobacter pylori. Protein Expression and Purification, 163, 1-8. https://doi.org/10.1016/j.pep.2019.105446

Helicobacter pylori is a pathogenic microorganism infecting approximately 50% of the global population, and establishes life-long colonization despite the hostile stomach environment. H. pylori employs a wide range of outer membrane proteins (adhesin... Read More about Introduction of a C-terminal hexa-lysine tag increases thermal stability of the LacDiNac binding adhesin (LabA) exodomain from Helicobacter pylori.

Mechanistic insight into heterogeneity of trans-plasma membrane electron transport in cancer cell types (2019)
Journal Article
Sherman, H. G., Jovanovic, C., Abuawad, A., Kim, D., Collins, H., Dixon, J. E., …Rawson, F. J. (2019). Mechanistic insight into heterogeneity of trans-plasma membrane electron transport in cancer cell types. BBA - Bioenergetics, 1860(8), 628-639. https://doi.org/10.1016/j.bbabio.2019.06.012

Trans-plasma membrane electron transfer (tMPET) is a process by which reducing equivalents, either electrons or reductants like ascorbic acid, are exported to the extracellular environment by the cell. TPMET is involved in a number of physiological p... Read More about Mechanistic insight into heterogeneity of trans-plasma membrane electron transport in cancer cell types.

In vitro investigation on the impact of airway mucus on drug dissolution and absorption at the air-epithelium interface in the lungs (2019)
Journal Article
Cingolani, E., Alqahtani, S., Sadler, R. C., Prime, D., Stolnik, S., & Bosquillon, C. (2019). In vitro investigation on the impact of airway mucus on drug dissolution and absorption at the air-epithelium interface in the lungs. European Journal of Pharmaceutics and Biopharmaceutics, 141, 210-220. https://doi.org/10.1016/j.ejpb.2019.05.022

Although the mucus layer is the first biological barrier encountered by inhaled drugs upon their deposition in the upper airways, its potential impact on drug dissolution and absorption in the lung has hardly been investigated. Bio-relevant in vitro... Read More about In vitro investigation on the impact of airway mucus on drug dissolution and absorption at the air-epithelium interface in the lungs.

Penetration and uptake of Nanoparticles in 3D tumour spheroids (2019)
Journal Article
Tchoryk, A., Taresco, V., Argent, R., Ashford, M. B., Gellert, P., Stolnik, S., …Garnett, M. C. (2019). Penetration and uptake of Nanoparticles in 3D tumour spheroids. Bioconjugate Chemistry, 30(5), 1371-1384. https://doi.org/10.1021/acs.bioconjchem.9b00136

Animal models are effective for assessing tumour localisation of nanosystems, but difficult to use for studying penetration beyond the vasculature. Here, we have used well-characterised HCT116 colorectal cancer spheroids to study the effect of nanopa... Read More about Penetration and uptake of Nanoparticles in 3D tumour spheroids.