Research Repository

See what's under the surface


Localized induction of gene expression in embryonic stem cell aggregates using holographic optical tweezers to create biochemical gradients (2019)
Journal Article
Kirkham, G. R., Ware, J., Upton, T., Allen, S., Shakesheff, K. M., & Buttery, L. D. (2019). Localized induction of gene expression in embryonic stem cell aggregates using holographic optical tweezers to create biochemical gradients. Regenerative Engineering and Translational Medicine, doi:10.1007/s40883-019-00114-5

Three dimensional (3D) cell models that mimic the structure and function of native tissues are enabling more detailed study of physiological and pathological mechanisms in vitro. We have previously demonstrated the ability to build and manipulate 3D... Read More

Highly versatile cell-penetrating peptide loaded scaffold for efficient and localised gene delivery to multiple cell types: From development to application in tissue engineering (2019)
Journal Article
Raftery, R. M., Walsh, D. P., Blokpoel Ferreras, L., Mencía Castaño, I., Chen, G., LeMoine, M., …O'Brien, F. J. (2019). Highly versatile cell-penetrating peptide loaded scaffold for efficient and localised gene delivery to multiple cell types: From development to application in tissue engineering. Biomaterials, 216, doi:10.1016/j.biomaterials.2019.119277

Gene therapy has recently come of age with seven viral vector-based therapies gaining regulatory approval in recent years. In tissue engineering, non-viral vectors are preferred over viral vectors, however, lower transfection efficiencies and difficu... Read More

Overall survival in malignant glioma is significantly prolonged by neurosurgical delivery of etoposide and temozolomide from a thermo-responsive biodegradable paste (2019)
Journal Article
Smith, S. J., Tyler, B., Gould, T. W., Veal, G. J., Gorelick, N. L., Rowlinson, J., …Rahman, R. (2019). Overall survival in malignant glioma is significantly prolonged by neurosurgical delivery of etoposide and temozolomide from a thermo-responsive biodegradable paste. Clinical Cancer Research, 25(16), 5094-5106. doi:10.1158/1078-0432.ccr-18-3850

Purpose: High-grade glioma (HGG) treatment is limited by the inability of otherwise potentially efficacious drugs to penetrate the blood brain barrier. We evaluate the unique intra-cavity delivery mode and translational potential of a blend of poly(D... Read More

3D printed scaffolds with controlled micro-/nano- porous surface topography direct chondrogenic and osteogenic differentiation of mesenchymal stem cells (2019)
Journal Article
Prasopthum, A., Cooper, M., Shakesheff, K. M., & Yang, J. (2019). 3D printed scaffolds with controlled micro-/nano- porous surface topography direct chondrogenic and osteogenic differentiation of mesenchymal stem cells. ACS Applied Materials and Interfaces, 11(21), 18896-18906. doi:10.1021/acsami.9b01472

The effect of topography in 3D printed polymer scaffolds on stem cell differentiation is a significantly under-explored area. Compared to 2D biomaterials on which various well-defined topographies have been incorporated and been shown to direct an ar... Read More

Microparticles for controlled GDF6 delivery to direct ASC‐based nucleus pulposus regeneration (2019)
Journal Article
Hodgkinson, T., Stening, J. Z., White, L. J., Shakesheff, K. M., Hoyland, J. A., & Richardson, S. M. (2019). Microparticles for controlled GDF6 delivery to direct ASC‐based nucleus pulposus regeneration. Journal of Tissue Engineering and Regenerative Medicine, 13(8), 1406-1417. doi:10.1002/term.2882

Currently, there is no effective long-term treatment for intervertebral disc degeneration (IVDD), making it an attractive candidate for regenerative therapies. Hydrogel delivery of adipose stem cells (ASCs) in combination with controlled release of b... Read More

Targeted protein delivery: carbodiimide crosslinking influences protein release from microparticles incorporated within collagen scaffolds (2019)
Journal Article
Tanase, C. E., Qutachi, O., White, L. J., Shakesheff, K. M., McCaskie, A. W., Best, S. M., & Cameron, R. E. (2019). Targeted protein delivery: carbodiimide crosslinking influences protein release from microparticles incorporated within collagen scaffolds. Regenerative Biomaterials, doi:10.1093/rb/rbz015

Tissue engineering response may be tailored via controlled, sustained release of active agents from protein loaded degradable microparticles incorporated directly within 3D ice-templated collagen scaffolds. However, the effects of covalent crosslink... Read More

Surface modification of PdlLGA microspheres with gelatine methacrylate: evaluation of adsorption, entrapment, and oxygen plasma treatment approaches (2017)
Journal Article
Baki, A., Rahman, C. V., White, L. J., Scurr, D. J., Qutachi, O., & Shakesheff, K. M. (2017). Surface modification of PdlLGA microspheres with gelatine methacrylate: evaluation of adsorption, entrapment, and oxygen plasma treatment approaches. Acta Biomaterialia, 53, 450-459. https://doi.org/10.1016/j.actbio.2017.01.042

Injectable poly (dl-lactic-co-glycolic acid) (PdlLGA) microspheres are promising candidates as biodegradable controlled release carriers for drug and cell delivery applications; however, they have limited functional groups on the surface to enable de... Read More

Characterisation of the surface structure of 3D printed scaffolds for cell infiltration and surgical suturing (2016)
Journal Article
Ruiz-Cantu, L., Gleadall, A., Faris, C., Segal, J., Shakesheff, K., & Yang, J. (2016). Characterisation of the surface structure of 3D printed scaffolds for cell infiltration and surgical suturing. Biofabrication, 8(1), https://doi.org/10.1088/1758-5090/8/1/015016

3D printing is of great interest for tissue engineering scaffolds due to the ability to form complex geometries and control internal structures, including porosity and pore size. The porous structure of scaffolds plays an important role in cell ingro... Read More

A thermoresponsive and magnetic colloid for 3D cell expansion and reconfiguration (2014)
Journal Article
Saeed, A. O., Francini, N., White, L., Dixon, J. E., Gould, T. W., Rashidi, H., …Shakesheff, K. (2015). A thermoresponsive and magnetic colloid for 3D cell expansion and reconfiguration. Advanced Materials, 27, doi:10.1002/adma.201403626

A dual thermoresponsive and magnetic colloidal gel matrix is described for enhanced stem-cell culture. The combined properties of the material allow enzyme-free passaging and expansion of mesenchymal stem cells, as well as isolation of cells postcult... Read More