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Comparative Hydrodynamic Study on Non-Aqueous Soluble Archaeological Wood Consolidants: Butvar B-98 and PDMS-OH Siloxanes (2022)
Journal Article
Cutajar, M., Stockman, R. A., Braovac, S., Steindal, C. C., Zisi, A., & Harding, S. E. (2022). Comparative Hydrodynamic Study on Non-Aqueous Soluble Archaeological Wood Consolidants: Butvar B-98 and PDMS-OH Siloxanes. Molecules, 27(7), Article 2133. https://doi.org/10.3390/molecules27072133

Butvar B-98 and PDMS-OH both have a demonstrable ability as consolidants for archaeological wood. This makes them both potential treatment options for the Oseberg collection, which is one of the most important archaeological finds from the Viking era... Read More about Comparative Hydrodynamic Study on Non-Aqueous Soluble Archaeological Wood Consolidants: Butvar B-98 and PDMS-OH Siloxanes.

Laboratory strains of Escherichia coli K-12: things are seldom what they seem (2022)
Journal Article
Browning, D. F., Hobman, J. L., & Busby, S. J. W. (2023). Laboratory strains of Escherichia coli K-12: things are seldom what they seem. Microbial Genomics, 9(2), Article 000922. https://doi.org/10.1099/mgen.0.000922

Escherichia coli K-12 was originally isolated 100 years ago and since then it has become an invaluable model organism and a cornerstone of molecular biology research. However, despite its pedigree, since its initial isolation E. coli K-12 has been re... Read More about Laboratory strains of Escherichia coli K-12: things are seldom what they seem.

Engineering bacteria to control electron transport altering the synthesis of non-native polymer (2021)
Journal Article
Bennett, M. R., Jain, A., Kovacs, K., Hill, P. J., Alexander, C., & Rawson, F. J. (2021). Engineering bacteria to control electron transport altering the synthesis of non-native polymer. RSC Advances, 12, 451-457. https://doi.org/10.1039/d1ra06403g

The use of bacteria as catalysts for radical polymerisations of synthetic monomers has recently been established. However, the role of trans Plasma Membrane Electron Transport (tPMET) in modulating these processes is not well understood. We sort to s... Read More about Engineering bacteria to control electron transport altering the synthesis of non-native polymer.

Identification and Categorization of Volatile Sulfur Flavor Compounds in Roasted Malts and Barley (2021)
Journal Article
Parr, H., Bolat, I., & Cook, D. (2023). Identification and Categorization of Volatile Sulfur Flavor Compounds in Roasted Malts and Barley. Journal of The American Society of Brewing Chemists, 81(1), 76-87. https://doi.org/10.1080/03610470.2021.2003669

We report for the first time the application of HS-SPME-GC coupled with sulfur-specific pulsed flame photometric detection to sensitively analyze the volatile sulfur compounds (VSC’s) present in drum roasted malt and barley samples typically used in... Read More about Identification and Categorization of Volatile Sulfur Flavor Compounds in Roasted Malts and Barley.

Impacts of Adjunct Incorporation on Flavor Stability Metrics at Early Stages of Beer Production (2021)
Journal Article
Maia, C., Cunha, S., Debyser, W., & Cook, D. (2023). Impacts of Adjunct Incorporation on Flavor Stability Metrics at Early Stages of Beer Production. Journal of The American Society of Brewing Chemists, 81(1), 54-65. https://doi.org/10.1080/03610470.2021.1993054

The fresh flavor of a beer brand is one of its most important quality parameters and should be retained as long as possible through shelf-life. Early stages of beer production can have significant impacts on beer flavor stability. The use of cereal a... Read More about Impacts of Adjunct Incorporation on Flavor Stability Metrics at Early Stages of Beer Production.

A TCATA by modality approach to study the multisensory temporal profile of hop bitter and flavour products applied in lager (2021)
Journal Article
Dietz, C., Cook, D., Yang, Q., Wilson, C., & Ford, R. (2022). A TCATA by modality approach to study the multisensory temporal profile of hop bitter and flavour products applied in lager. Food Quality and Preference, 97, Article 104470. https://doi.org/10.1016/j.foodqual.2021.104470

Previous research suggested that iso-alpha-acids and hulupones add different bitterness profiles to beer and hop-derived volatiles modify temporal dimensions of bitterness qualities via cross-modal interactions. This research aimed to understand the... Read More about A TCATA by modality approach to study the multisensory temporal profile of hop bitter and flavour products applied in lager.

Venatorbacter cucullus gen. nov sp. nov a novel bacterial predator (2021)
Journal Article
Saeedi, A., Cummings, N. J., McLean, D., Connerton, I. F., & Connerton, P. L. (2021). Venatorbacter cucullus gen. nov sp. nov a novel bacterial predator. Scientific Reports, 11, Article 21393. https://doi.org/10.1038/s41598-021-00865-8

A novel Gram-stain negative, aerobic, halotolerant, motile, rod-shaped, predatory bacterium ASxL5T, was isolated from a bovine slurry tank in Nottinghamshire, UK using Campylobacter hyointestinalis as prey. Other Campylobacter species and members of... Read More about Venatorbacter cucullus gen. nov sp. nov a novel bacterial predator.

Tackling protozoan parasites of cattle in sub-Saharan Africa (2021)
Journal Article
MacGregor, P., Nene, V., & Nisbet, R. E. R. (2021). Tackling protozoan parasites of cattle in sub-Saharan Africa. PLoS Pathogens, 17(10), Article e1009955. https://doi.org/10.1371/journal.ppat.1009955

Cattle are an incredibly valuable asset to farmers throughout the world (Fig 1). They provide power, transport, fertiliser, fuel, and nutrition. In some areas, cattle guarantee a family’s food and economic security and act as important indicators of... Read More about Tackling protozoan parasites of cattle in sub-Saharan Africa.

Thermodynamics of semi-specific ligand recognition: the binding of dipeptides to the E.coli dipeptide binding protein DppA (2021)
Journal Article
Zainol, M. K., Linforth, R. J., Winzor, D. J., & Scott, D. J. (2021). Thermodynamics of semi-specific ligand recognition: the binding of dipeptides to the E.coli dipeptide binding protein DppA. European Biophysics Journal, 50(8), 1103-1110. https://doi.org/10.1007/s00249-021-01572-y

This investigation of the temperature dependence of DppA interactions with a subset of three dipeptides (AA. AF and FA) by isothermal titration calorimetry has revealed the negative heat capacity (ΔCpo) that is a characteristic of hydrophobic interac... Read More about Thermodynamics of semi-specific ligand recognition: the binding of dipeptides to the E.coli dipeptide binding protein DppA.

Occurrence and distribution of antibiotics and antibiotic resistance determinants in coastal environments (2021)
Book Chapter
Baena-Nogueras, R. M., Hobman, J. L., & Gomes, R. L. (2021). Occurrence and distribution of antibiotics and antibiotic resistance determinants in coastal environments. In Pharmaceuticals in Marine and Coastal Environments: Occurrence, Effects and Challenges in a Changing World (121-167). Elsevier. https://doi.org/10.1016/b978-0-08-102971-8.00008-1

The occurrence of antibiotic compounds in coastal environments is of great concern since most of the pollutant discharges flow into the ocean. The immediate effect of antibiotic release in marine environments is believed to be the selection of resist... Read More about Occurrence and distribution of antibiotics and antibiotic resistance determinants in coastal environments.

Characterisation of mass distributions of solvent-fractionated lignins using analytical ultracentrifugation and size exclusion chromatography methods (2021)
Journal Article
Lu, Y., Joosten, L., Donkers, J., Andriulo, F., Slaghek, T. M., Phillips-Jones, M. K., …Harding, S. E. (2021). Characterisation of mass distributions of solvent-fractionated lignins using analytical ultracentrifugation and size exclusion chromatography methods. Scientific Reports, 11(1), 1-12. https://doi.org/10.1038/s41598-021-93424-0

Lignins are valuable renewable resources for the potential production of a large array of biofuels, aromatic chemicals and biopolymers. Yet native and industrial lignins are complex, highly branched and heterogenous macromolecules, properties that ha... Read More about Characterisation of mass distributions of solvent-fractionated lignins using analytical ultracentrifugation and size exclusion chromatography methods.

The Evolution of the Cytochrome c6 Family of Photosynthetic Electron Transfer Proteins (2021)
Journal Article
Slater, B., Kosmützky, D., Nisbet, E. R., & Howe, C. J. (2021). The Evolution of the Cytochrome c6 Family of Photosynthetic Electron Transfer Proteins. Genome Biology and Evolution, 13(8), Article evab146. https://doi.org/10.1093/gbe/evab146

During photosynthesis, electrons are transferred between the cytochrome b6f complex and photosystem I. This is carried out by the protein plastocyanin in plant chloroplasts, or by either plastocyanin or cytochrome c6 in many cyanobacteria and eukaryo... Read More about The Evolution of the Cytochrome c6 Family of Photosynthetic Electron Transfer Proteins.

Influence of essential inorganic elements on flavour formation during yeast fermentation (2021)
Journal Article
Ribeiro-Filho, N., Linforth, R., Powell, C. D., & Fisk, I. D. (2021). Influence of essential inorganic elements on flavour formation during yeast fermentation. Food Chemistry, 361, Article 130025. https://doi.org/10.1016/j.foodchem.2021.130025

The relative concentration of available inorganic elements is critical for yeast growth and metabolism and has potential to be a tool leading to directed yeast flavour formation during fermentation. This study investigates the influence of essential... Read More about Influence of essential inorganic elements on flavour formation during yeast fermentation.

The influence of yeast strain on the oxidative stability of beer (2021)
Journal Article
James, S., Jenkins, D., Dehrmann, F., Smart, K., & Cook, D. (2021). The influence of yeast strain on the oxidative stability of beer. Journal of the Institute of Brewing, 127(3), 248-255. https://doi.org/10.1002/jib.650

Flavour stability, or instability, relates to the rate of flavour change through shelf-life of packaged beer. There are several control points in the production of beer where flavour stability may be altered. These include fermentation and the influe... Read More about The influence of yeast strain on the oxidative stability of beer.

Green Malt for a Green Future – Feasibility and Challenges of Brewing Using Freshly Germinated (Unkilned) Malt: A Review (2021)
Journal Article
Dugulin, C. A., De Rouck, G., & Cook, D. J. (2021). Green Malt for a Green Future – Feasibility and Challenges of Brewing Using Freshly Germinated (Unkilned) Malt: A Review. Journal of The American Society of Brewing Chemists, 79(4), 315-332. https://doi.org/10.1080/03610470.2021.1902710

In malting, the kilning step is by far the dominant user of energy and has thus become the main target in reducing the carbon footprint of malting operations. Brewing beer using ‘green’ (germinated, but not dried) malt, thus saving the substantial en... Read More about Green Malt for a Green Future – Feasibility and Challenges of Brewing Using Freshly Germinated (Unkilned) Malt: A Review.

Marine-Source Quorum Quenching Enzyme YtnP to Improve Hygiene Quality in Dental Units (2021)
Journal Article
Sun, X., Hill, P., Liu, J., Qian, J., Ma, Y., & Zhou, S. (2021). Marine-Source Quorum Quenching Enzyme YtnP to Improve Hygiene Quality in Dental Units. Marine Drugs, 19(4), Article 225. https://doi.org/10.3390/md19040225

Biofilm in dental unit water lines may pose a health risk to patients and dental practitioners. An AdiC-like quorum quenching enzyme, YtnP, was cloned from a deep-sea probiotic Bacillus velezensis, and heterologously expressed in E. coli to examine t... Read More about Marine-Source Quorum Quenching Enzyme YtnP to Improve Hygiene Quality in Dental Units.

Quantifying the concentration dependence of sedimentation coefficients for globular macromolecules: a continuing age-old problem (2021)
Journal Article
Winzor, D. J., Dinu, V., Scott, D. J., & Harding, S. E. (2021). Quantifying the concentration dependence of sedimentation coefficients for globular macromolecules: a continuing age-old problem. Biophysical Reviews, 13(2), 273-288. https://doi.org/10.1007/s12551-021-00793-x

This retrospective investigation has established that the early theoretical attempts to directly incorporate the consequences of radial dilution into expressions for variation of the sedimentation coefficient as a function of the loading concentratio... Read More about Quantifying the concentration dependence of sedimentation coefficients for globular macromolecules: a continuing age-old problem.

Hybrid assembly of an agricultural slurry virome reveals a diverse and stable community with the potential to alter the metabolism and virulence of veterinary pathogens (2021)
Journal Article
Cook, R., Hooton, S., Trivedi, U., King, L., Dodd, C. E., Hobman, J. L., …Millard, A. D. (2021). Hybrid assembly of an agricultural slurry virome reveals a diverse and stable community with the potential to alter the metabolism and virulence of veterinary pathogens. Microbiome, 9, Article 65. https://doi.org/10.1186/s40168-021-01010-3

Viruses are the most abundant biological entities on Earth, known to be crucial components of microbial ecosystems. However, there is little information on the viral community within agricultural waste. There are currently ~ 2.7 million dairy cattle... Read More about Hybrid assembly of an agricultural slurry virome reveals a diverse and stable community with the potential to alter the metabolism and virulence of veterinary pathogens.

Towards a general model for predicting minimal metal concentrations co-selecting for antibiotic resistance plasmids (2021)
Journal Article
Arya, S., Williams, A., Reina, S. V., Knapp, C. W., Kreft, J., Hobman, J. L., & Stekel, D. J. (2021). Towards a general model for predicting minimal metal concentrations co-selecting for antibiotic resistance plasmids. Environmental Pollution, 275, Article 116602. https://doi.org/10.1016/j.envpol.2021.116602

© 2021 Elsevier Ltd Many antibiotic resistance genes co-occur with resistance genes for transition metals, such as copper, zinc, or mercury. In some environments, a positive correlation between high metal concentration and high abundance of antibioti... Read More about Towards a general model for predicting minimal metal concentrations co-selecting for antibiotic resistance plasmids.