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eIF4A1-dependent mRNAs employ purine-rich 5'UTR sequences to activate localised eIF4A1-unwinding through eIF4A1-multimerisation to facilitate translation (2023)
Journal Article
Schmidt, T., Dabrowska, A., Waldron, J. A., Hodge, K., Koulouras, G., Gabrielsen, M., …Bushell, M. (2023). eIF4A1-dependent mRNAs employ purine-rich 5'UTR sequences to activate localised eIF4A1-unwinding through eIF4A1-multimerisation to facilitate translation. Nucleic Acids Research, 51(4), 1859-1879. https://doi.org/10.1093/nar/gkad030

Altered eIF4A1 activity promotes translation of highly structured, eIF4A1-dependent oncogene mRNAs at root of oncogenic translational programmes. It remains unclear how these mRNAs recruit and activate eIF4A1 unwinding specifically to facilitate thei... Read More about eIF4A1-dependent mRNAs employ purine-rich 5'UTR sequences to activate localised eIF4A1-unwinding through eIF4A1-multimerisation to facilitate translation.

Multidrug-Resistant ESBL-Producing E. coli in Clinical Samples from the UK (2023)
Journal Article
Ibrahim, D. R., Dodd, C. E. R., Stekel, D. J., Meshioye, R. T., Diggle, M., Lister, M., & Hobman, J. L. (2023). Multidrug-Resistant ESBL-Producing E. coli in Clinical Samples from the UK. Antibiotics, 12(1), Article 169. https://doi.org/10.3390/antibiotics12010169

Globally, cephalosporin therapy failure is a serious problem for infection control. One causative agent of cephalosporin-resistant infections is multidrug-resistant (MDR) E. coli producing extended-spectrum β-lactamases (ESBLs) and/or plasmid-encoded... Read More about Multidrug-Resistant ESBL-Producing E. coli in Clinical Samples from the UK.

Establishment of a novel set of vectors for transformation of the dinoflagellate Amphidinium carterae (2022)
Working Paper
Nimmo, I., Geisler, K., Barbrook, A., Kleiner, F., Scarampi, A., Kosmützky, D., …Howe, C. Establishment of a novel set of vectors for transformation of the dinoflagellate Amphidinium carterae

Peridinin-containing dinoflagellate algae have a chloroplast genome formed from plasmid-like minicircles. This fragmented genome has allowed us to develop a genetic modification methodology involving the use of biolistics to introduce artificial mini... Read More about Establishment of a novel set of vectors for transformation of the dinoflagellate Amphidinium carterae.

Comparative hydrodynamic characterisation of two hydroxylated polymers based on α-pinene- or oleic acid-derived monomers for potential use as archaeological consolidants (2022)
Journal Article
Cutajar, M., Machado, F., Cuzzucoli Crucitti, V., Braovac, S., Stockman, R. A., Howdle, S. M., & Harding, S. E. (2022). Comparative hydrodynamic characterisation of two hydroxylated polymers based on α-pinene- or oleic acid-derived monomers for potential use as archaeological consolidants. Scientific Reports, 12(1), Article 18411. https://doi.org/10.1038/s41598-022-21027-4

The Oseberg Viking ship burial is one of the most extensive collections of Viking wooden artefacts ever excavated in Norway. In the early twentieth century, many of these artefacts were treated with alum in order to preserve them, inadvertently leadi... Read More about Comparative hydrodynamic characterisation of two hydroxylated polymers based on α-pinene- or oleic acid-derived monomers for potential use as archaeological consolidants.

Gut transcriptome reveals differential gene expression and enriched pathways linked to immune activation in response to weaning in pigs (2022)
Journal Article
Le Bon, M., Tötemeyer, S., Emes, R. D., & Mellits, K. H. (2022). Gut transcriptome reveals differential gene expression and enriched pathways linked to immune activation in response to weaning in pigs. Frontiers in Genetics, 13, Article 961474. https://doi.org/10.3389/fgene.2022.961474

Weaning represents one of the most critical periods in pig production associated with increase in disease risk, reduction in performance and economic loss. Physiological changes faced by piglets during the weaning period have been well characterised,... Read More about Gut transcriptome reveals differential gene expression and enriched pathways linked to immune activation in response to weaning in pigs.

Synthetic Biology Toolbox, Including a Single-Plasmid CRISPR-Cas9 System to Biologically Engineer the Electrogenic, Metal-Resistant Bacterium Cupriavidus metallidurans CH34 (2022)
Journal Article
Turco, F., Garavaglia, M., Van Houdt, R., Hill, P., Rawson, F. J., & Kovacs, K. (2022). Synthetic Biology Toolbox, Including a Single-Plasmid CRISPR-Cas9 System to Biologically Engineer the Electrogenic, Metal-Resistant Bacterium Cupriavidus metallidurans CH34. ACS Synthetic Biology, https://doi.org/10.1021/acssynbio.2c00130

Cupriavidus metallidurans CH34 exhibits extraordinary metabolic versatility, including chemolithoautotrophic growth; degradation of BTEX (benzene, toluene, ethylbenzene, xylene); high resistance to numerous metals; biomineralization of gold, platinum... Read More about Synthetic Biology Toolbox, Including a Single-Plasmid CRISPR-Cas9 System to Biologically Engineer the Electrogenic, Metal-Resistant Bacterium Cupriavidus metallidurans CH34.

The role of inorganic-phosphate, potassium and magnesium in yeast-flavour formation (2022)
Journal Article
Ribeiro-Filho, N., Linforth, R., Bora, N., Powell, C. D., & Fisk, I. D. (2022). The role of inorganic-phosphate, potassium and magnesium in yeast-flavour formation. Food Research International, 162, Article 112044. https://doi.org/10.1016/j.foodres.2022.112044

Inorganic-phosphate, potassium, and magnesium are key-minerals required for yeast growth, metabolism, and survival, the present work investigated its impact in yeast-flavour formation using a multi-factor experimental design, which was used to genera... Read More about The role of inorganic-phosphate, potassium and magnesium in yeast-flavour formation.

Enhancing Microbial Electron Transfer through Synthetic Biology and Biohybrid Approaches: Part I: Bioelectrochemistry for sustainable energy conversion (2022)
Journal Article
Myers, B., Hill, P., Rawson, F., & Kovacs, K. (2022). Enhancing Microbial Electron Transfer through Synthetic Biology and Biohybrid Approaches: Part I: Bioelectrochemistry for sustainable energy conversion. Johnson Matthey Technology Review, 66(4), 443-454. https://doi.org/10.1595/205651322X16548607638938

Traditional microbial synthesis of chemicals and fuels often rely on energy-rich feedstocks such as glucose, raising ethical concerns as they are directly competing with the food supply. Therefore, it is imperative to develop novel processes that rel... Read More about Enhancing Microbial Electron Transfer through Synthetic Biology and Biohybrid Approaches: Part I: Bioelectrochemistry for sustainable energy conversion.

Non-starch polysaccharides in beer and brewing: A review of their occurrence and significance (2022)
Journal Article
Reid, J. E. S. J., Yakubov, G. E., & Lawrence, S. J. (2024). Non-starch polysaccharides in beer and brewing: A review of their occurrence and significance. Critical Reviews in Food Science and Nutrition, 64, 837-851. https://doi.org/10.1080/10408398.2022.2109585

It has become apparent that beer (both alcoholic and nonalcoholic) contains appreciable amounts of non-starch polysaccharides, a broad subgroup of dietary fiber. It is worth noting that the occurrence of non-starch polysaccharides in alcoholic beer d... Read More about Non-starch polysaccharides in beer and brewing: A review of their occurrence and significance.

The effects of exogenous fibrolytic enzymes on the in vitro generation of xylooligosaccharides and monosaccharides is dependent upon cereal type (2022)
Journal Article
Dale, T., Bedford, M. R., Tucker, G. A., Brameld, J. M., & Parr, T. (2022). The effects of exogenous fibrolytic enzymes on the in vitro generation of xylooligosaccharides and monosaccharides is dependent upon cereal type. Animal Feed Science and Technology, 292, Article 115406. https://doi.org/10.1016/j.anifeedsci.2022.115406

Fibrolytic enzymes are routinely added to non-ruminant livestock feeds to help degrade the non-starch polysaccharide (NSP) contents and thereby improve feed efficiency. This study investigated the range of xylooligosaccharides (XOS) and monosaccharid... Read More about The effects of exogenous fibrolytic enzymes on the in vitro generation of xylooligosaccharides and monosaccharides is dependent upon cereal type.

Oxygen-Tolerant RAFT Polymerization Initiated by Living Bacteria (2022)
Journal Article
Bennett, M. R., Moloney, C., Catrambone, F., Turco, F., Myers, B., Kovacs, K., …Gurnani, P. (2022). Oxygen-Tolerant RAFT Polymerization Initiated by Living Bacteria. ACS Macro Letters, 11(8), 954-960. https://doi.org/10.1021/acsmacrolett.2c00372

Living organisms can synthesize a wide range of macromolecules from a small set of natural building blocks, yet there is potential for even greater materials diversity by exploiting biochemical processes to convert unnatural feedstocks into new abiot... Read More about Oxygen-Tolerant RAFT Polymerization Initiated by Living Bacteria.

Oxygen-Tolerant RAFT Polymerization Initiated by Living Bacteria (2022)
Journal Article
Gurnani, P., Bennett, M. R., Moloney, C., Catrambone, F., Turco, F., Myers, B., …Rawson, F. J. (2022). Oxygen-Tolerant RAFT Polymerization Initiated by Living Bacteria. ACS Macro Letters, 11(8), 954-960. https://doi.org/10.1021/acsmacrolett.2c00372

Living organisms can synthesize a wide range of macromolecules from a small set of natural building blocks, yet there is potential for even greater materials diversity by exploiting biochemical processes to convert unnatural feedstocks into new abiot... Read More about Oxygen-Tolerant RAFT Polymerization Initiated by Living Bacteria.

Laboratory strains of Escherichia coli K-12: not such perfect role models after all (2022)
Working Paper
Browning, D. F., Hobman, J. L., & Busby, S. J. Laboratory strains of Escherichia coli K-12: not such perfect role models after all

Escherichia coli K-12 was originally isolated 100 years ago and since then, it has become an invaluable model organism and a cornerstone of molecular biology research. However, despite its apparent pedigree, since its initial isolation, E. coli K-12... Read More about Laboratory strains of Escherichia coli K-12: not such perfect role models after all.

In silico identification of Theileria parva surface proteins (2022)
Journal Article
Gurav, N., Macleod, O. J., MacGregor, P., & Nisbet, R. E. R. (2022). In silico identification of Theileria parva surface proteins. The Cell Surface, 8, Article 100078. https://doi.org/10.1016/j.tcsw.2022.100078

East Coast Fever is a devastating African cattle disease caused by the apicomplexan parasite, Theileria parva. Little is known about the cell surface, and few proteins have been identified. Here, we take an in silico approach to identify novel cell s... Read More about In silico identification of Theileria parva surface proteins.

Common colonic community indicators of the suckling pig microbiota where diversity and abundance correlate with performance (2022)
Journal Article
Lee, A., Le Bon, M., Connerton, I. F., & Mellits, K. H. (2022). Common colonic community indicators of the suckling pig microbiota where diversity and abundance correlate with performance. FEMS Microbiology Ecology, 98(5), Article fiac048. https://doi.org/10.1093/femsec/fiac048

The primary objective of this study was to investigate if common colonic community indicators could be identified from the microbiota of 22-day-old suckling pigs in repeated small-scale trials. A total of three separate trials were conducted at diffe... Read More about Common colonic community indicators of the suckling pig microbiota where diversity and abundance correlate with performance.

Flavour compounds affect protein structure: The effect of methyl anthranilate on bovine serum albumin conformation (2022)
Journal Article
Dinu, V., Borah, P. K., Muleya, M., Scott, D. J., Lithgo, R., Pattem, J., …Fisk, I. D. (2022). Flavour compounds affect protein structure: The effect of methyl anthranilate on bovine serum albumin conformation. Food Chemistry, 388, Article 133013. https://doi.org/10.1016/j.foodchem.2022.133013

This study aims to understand possible effects of flavour compounds on the structure and conformation of endogenous proteins. Using methyl anthranilate (a grape flavour compound added to drinks, confectionery, and vape-liquids) and bovine serum album... Read More about Flavour compounds affect protein structure: The effect of methyl anthranilate on bovine serum albumin conformation.

Instrumental characterization of xanthan gum and scleroglucan solutions: Comparison of rotational rheometry, capillary breakup extensional rheometry and soft-contact tribology (2022)
Journal Article
Li, X., Harding, S. E., Wolf, B., & Yakubov, G. E. (2022). Instrumental characterization of xanthan gum and scleroglucan solutions: Comparison of rotational rheometry, capillary breakup extensional rheometry and soft-contact tribology. Food Hydrocolloids, 130, Article 107681. https://doi.org/10.1016/j.foodhyd.2022.107681

Xanthan gum and scleroglucan, two rod-like polysaccharide hydrocolloids, are compared using a wide range of instrumental techniques and methods: steady shear flow, small amplitude oscillatory shear, first normal stress difference, capillary break-up... Read More about Instrumental characterization of xanthan gum and scleroglucan solutions: Comparison of rotational rheometry, capillary breakup extensional rheometry and soft-contact tribology.

Comparative Hydrodynamic Study on Non-Aqueous Soluble Archaeological Wood Consolidants: Butvar B-98 and PDMS-OH Siloxanes (2022)
Journal Article
Cutajar, M., Stockman, R. A., Braovac, S., Steindal, C. C., Zisi, A., & Harding, S. E. (2022). Comparative Hydrodynamic Study on Non-Aqueous Soluble Archaeological Wood Consolidants: Butvar B-98 and PDMS-OH Siloxanes. Molecules, 27(7), Article 2133. https://doi.org/10.3390/molecules27072133

Butvar B-98 and PDMS-OH both have a demonstrable ability as consolidants for archaeological wood. This makes them both potential treatment options for the Oseberg collection, which is one of the most important archaeological finds from the Viking era... Read More about Comparative Hydrodynamic Study on Non-Aqueous Soluble Archaeological Wood Consolidants: Butvar B-98 and PDMS-OH Siloxanes.

Laboratory strains of Escherichia coli K-12: things are seldom what they seem (2022)
Journal Article
Browning, D. F., Hobman, J. L., & Busby, S. J. W. (2023). Laboratory strains of Escherichia coli K-12: things are seldom what they seem. Microbial Genomics, 9(2), Article 000922. https://doi.org/10.1099/mgen.0.000922

Escherichia coli K-12 was originally isolated 100 years ago and since then it has become an invaluable model organism and a cornerstone of molecular biology research. However, despite its pedigree, since its initial isolation E. coli K-12 has been re... Read More about Laboratory strains of Escherichia coli K-12: things are seldom what they seem.

Engineering bacteria to control electron transport altering the synthesis of non-native polymer (2021)
Journal Article
Bennett, M. R., Jain, A., Kovacs, K., Hill, P. J., Alexander, C., & Rawson, F. J. (2021). Engineering bacteria to control electron transport altering the synthesis of non-native polymer. RSC Advances, 12, 451-457. https://doi.org/10.1039/d1ra06403g

The use of bacteria as catalysts for radical polymerisations of synthetic monomers has recently been established. However, the role of trans Plasma Membrane Electron Transport (tPMET) in modulating these processes is not well understood. We sort to s... Read More about Engineering bacteria to control electron transport altering the synthesis of non-native polymer.