Skip to main content

Research Repository

Advanced Search

All Outputs (34)

Cell imaging by phonon microscopy: sub-optical wavelength ultrasound for non-invasive imaging (2017)
Journal Article
Perez-Cota, F., Smith, R. J., Moradi, E., Leija, A. L., Velickovic, K., Marques, L., …Clark, M. (2017). Cell imaging by phonon microscopy: sub-optical wavelength ultrasound for non-invasive imaging

The mechanical properties of cells play an important role in cell function and behavior. This paper presents recent developments that have enabled the use of laser-generated phonons (ultrasound) with sub-optical wavelengths to look inside living cell... Read More about Cell imaging by phonon microscopy: sub-optical wavelength ultrasound for non-invasive imaging.

Expression of a SOX1 overlapping transcript in neural differentiation and cancer models (2017)
Journal Article
Azaz, A., Strohbuecker, S., Tufarelli, C., & Sottile, V. (2017). Expression of a SOX1 overlapping transcript in neural differentiation and cancer models. Cellular and Molecular Life Sciences, 74(22),

SOX1 is a member of the SOXB1 subgroup of transcription factors involved in early embryogenesis, CNS development and maintenance of neural stem cells. The structure and regulation of the human SOX1 locus has been less studied than that of SOX2, anoth... Read More about Expression of a SOX1 overlapping transcript in neural differentiation and cancer models.

Autonomous magnetic labelling of functional mesenchymal stem cells for improved traceability and spatial control in cell therapy applications (2016)
Journal Article
Harrison, R., Markides, H., Morris, R. H., Richards, P., El Haj, A. J., & Sottile, V. (2017). Autonomous magnetic labelling of functional mesenchymal stem cells for improved traceability and spatial control in cell therapy applications. Journal of Tissue Engineering and Regenerative Medicine, 11(8), https://doi.org/10.1002/term.2133

Mesenchymal stem cells (MSCs) represent a valuable resource for regenerative medicine treatments for orthopaedic repair and beyond. Following developments in isolation, expansion and differentiation protocols, efforts to promote clinical translation... Read More about Autonomous magnetic labelling of functional mesenchymal stem cells for improved traceability and spatial control in cell therapy applications.

Non-destructive characterisation of mesenchymal stem cell differentiation using LC-MS-based metabolite footprinting (2016)
Journal Article
Surrati, A., Linforth, R. S., Fisk, I. D., Sottile, V., & Kim, D. (in press). Non-destructive characterisation of mesenchymal stem cell differentiation using LC-MS-based metabolite footprinting. Analyst, 12, https://doi.org/10.1039/c6an00170j

Bone regeneration is a complex biological process where major cellular changes take place to support the osteogenic differentiation of mesenchymal bone progenitors. To characterise these biological changes and better understand the pathways regulatin... Read More about Non-destructive characterisation of mesenchymal stem cell differentiation using LC-MS-based metabolite footprinting.

In vitro degradation and mechanical properties of PLA-PCL copolymer unit cell scaffolds generated by two-photon polymerization (2016)
Journal Article
Felfel, R. M., Poocza, L., Gimeno-Fabra, M., Milde, T., Hildebrand, G., Ahmed, I., …Liefeith, K. (2016). In vitro degradation and mechanical properties of PLA-PCL copolymer unit cell scaffolds generated by two-photon polymerization. Biomedical Materials, 11(1), Article 015011. https://doi.org/10.1088/1748-6041/11/1/015011

© 2016 IOP Publishing Ltd. The manufacture of 3D scaffolds with specific controlled porous architecture, defined microstructure and an adjustable degradation profile was achieved using two-photon polymerization (TPP) with a size of 2 × 4 × 2 mm3. Sca... Read More about In vitro degradation and mechanical properties of PLA-PCL copolymer unit cell scaffolds generated by two-photon polymerization.

Towards quantitative molecular mapping of cells by Raman microscopy: using AFM for decoupling molecular concentration and cell topography (2015)
Journal Article
Boitor, R., Sinjab, F., Strohbuecker, S., Sottile, V., & Notingher, I. (2016). Towards quantitative molecular mapping of cells by Raman microscopy: using AFM for decoupling molecular concentration and cell topography. Faraday Discussions, 187, https://doi.org/10.1039/C5FD00172B

Raman micro-spectroscopy (RMS) is a non-invasive technique for imaging live cells in-vitro. However, obtaining quantitative molecular information from the Raman spectra is difficult because the intensity of a Raman band is proportional to the number... Read More about Towards quantitative molecular mapping of cells by Raman microscopy: using AFM for decoupling molecular concentration and cell topography.

Applications of Raman micro-spectroscopy to stem cell technology: label-free molecular discrimination and monitoring cell differentiation. (2015)
Journal Article
Ghita, A., Pascut, F. C., Sottile, V., Denning, C., & Notingher, I. (2015). Applications of Raman micro-spectroscopy to stem cell technology: label-free molecular discrimination and monitoring cell differentiation. EPJ Techniques and Instrumentation, 2, Article 6. https://doi.org/10.1140/epjti/s40485-015-0016-8

Stem cell therapy is widely acknowledged as a key medical technology of the 21st century which may provide treatments for many currently incurable diseases. These cells have an enormous potential for cell replacement therapies to cure diseases such a... Read More about Applications of Raman micro-spectroscopy to stem cell technology: label-free molecular discrimination and monitoring cell differentiation..

Transient Accumulation of 5-Carboxylcytosine Indicates Involvement of Active Demethylation in Lineage Specification of Neural Stem Cells (2014)
Journal Article
Wheldon, L. M., Abakir, A., Ferjentsik, Z., Dudnakova, T., Strohbuecker, S., Christie, D., …Ruzov, A. (2014). Transient Accumulation of 5-Carboxylcytosine Indicates Involvement of Active Demethylation in Lineage Specification of Neural Stem Cells. Cell Reports, 7(5), 1353-1361. https://doi.org/10.1016/j.celrep.2014.05.003

5-Methylcytosine (5mC) is an epigenetic modification involved in regulation of gene activity during differentiation. Tet dioxygenases oxidize 5mC to 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC), and 5-carboxylcytosine (5caC). Both 5fC and 5... Read More about Transient Accumulation of 5-Carboxylcytosine Indicates Involvement of Active Demethylation in Lineage Specification of Neural Stem Cells.

Monitoring the mineralisation of bone nodules in vitro by space- and time-resolved Raman micro-spectroscopy. (2013)
Journal Article
Ghita, A., Pascut, F. C., Sottile, V., & Notingher, I. (2013). Monitoring the mineralisation of bone nodules in vitro by space- and time-resolved Raman micro-spectroscopy. Analyst, 139(1), https://doi.org/10.1039/C3AN01716H

Raman microscopy was used as a label-free method to study the mineralisation of bone nodules formed by mesenchymal stem cells cultured in osteogenic medium in vitro. Monitoring individual bone nodules over 28 days revealed temporal and spatial change... Read More about Monitoring the mineralisation of bone nodules in vitro by space- and time-resolved Raman micro-spectroscopy..

Transient serum exposure regimes to support dual differentiation of human mesenchymal stem cells (2012)
Journal Article
France, L., Scotchford, C., Grant, D., Rashidi, H., Popov, A., & Sottile, V. (2014). Transient serum exposure regimes to support dual differentiation of human mesenchymal stem cells. Journal of Tissue Engineering and Regenerative Medicine, 8(8), https://doi.org/10.1002/term.1567

Human mesenchymal stem cells (MSCs), which can generate both osteoblasts and chondrocytes, represent an ideal resource for orthopaedic repair using tissue-engineering approaches. One major difficulty for the development of osteochondral constructs us... Read More about Transient serum exposure regimes to support dual differentiation of human mesenchymal stem cells.

5-hydroxymethyl-cytosine enrichment of non-committed cells is not a universal feature of vertebrate development (2012)
Journal Article
Almeida, R. D., Loose, M., Sottile, V., Matsa, E., Denning, C., Young, L., …Ruzov, A. (2012). 5-hydroxymethyl-cytosine enrichment of non-committed cells is not a universal feature of vertebrate development. Epigenetics, 7(4), https://doi.org/10.4161/epi.19375

5-hydroxymethyl-cytosine (5-hmc) is a cytosine modification that is relatively abundant in mammalian pre-implantation embryos and embryonic stem cells (Esc) derived from mammalian blastocysts. Recent observations imply that both 5-hmc and Tet1/2/... Read More about 5-hydroxymethyl-cytosine enrichment of non-committed cells is not a universal feature of vertebrate development.

Cytoplasmic RNA in undifferentiated neural stem cells: a potential label-free Raman spectral marker for assessing the undifferentiated status (2012)
Journal Article
Ghita, A., Pascut, F. C., Mather, M., Sottile, V., & Notingher, I. (2012). Cytoplasmic RNA in undifferentiated neural stem cells: a potential label-free Raman spectral marker for assessing the undifferentiated status. Analytical Chemistry, 84(7), https://doi.org/10.1021/ac202994e

Raman microspectroscopy (rms) was used to identify, image, and quantify potential molecular markers for label-free monitoring the differentiation status of live neural stem cells (NSCs) in vitro. Label-free noninvasive techniques for characterization... Read More about Cytoplasmic RNA in undifferentiated neural stem cells: a potential label-free Raman spectral marker for assessing the undifferentiated status.

Semi-quantitative immunohistochemical detection of 5-hydroxymethyl-cytosine reveals conservation of its tissue distribution between amphibians and mammals (2012)
Journal Article
Almeida, R. D., Sottile, V., Loose, M., De Sousa, P. A., Johnson, A. D., & Ruzov, A. (2012). Semi-quantitative immunohistochemical detection of 5-hydroxymethyl-cytosine reveals conservation of its tissue distribution between amphibians and mammals. Epigenetics, 7(2), https://doi.org/10.4161/epi.7.2.18949

5-Hydroxymethyl-cytosine (5-hmC) is a form of modified cytosine, which has recently attracted a considerable attention due to its potential role in transcriptional regulation. According to several reports 5-hydroxymethyl-cytosine distribution is tiss... Read More about Semi-quantitative immunohistochemical detection of 5-hydroxymethyl-cytosine reveals conservation of its tissue distribution between amphibians and mammals.

Differences in the pattern and regulation of mineral deposition in human cell lines of osteogenic and non-osteogenic origin (2011)
Journal Article
Rashidi, H., Strohbuecker, S., Jackson, L., Kalra, S., Blake, A. J., France, L., …Sottile, V. (2011). Differences in the pattern and regulation of mineral deposition in human cell lines of osteogenic and non-osteogenic origin. Cells Tissues Organs, 195(6), https://doi.org/10.1159/000329861

Bone marrow-derived mesenchymal stem cells (MSCs) are widely used as a cellular model of bone formation, and can mineralize in vitro in response to osteogenic medium (OM). It is unclear, however, whether this property is specific to cells of mesenchy... Read More about Differences in the pattern and regulation of mineral deposition in human cell lines of osteogenic and non-osteogenic origin.