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Effect of adeno-associated virus (AAV)-mediated overexpression of PEPCK-M (Pck2) on Clenbuterol-induced muscle growth (2019)
Journal Article
Loczenski-Brown, D. M., Jones, S., Luckett, J., Daniel, Z., Brearley, M. C., Ebling, F. J. P., …Brameld, J. M. (2019). Effect of adeno-associated virus (AAV)-mediated overexpression of PEPCK-M (Pck2) on Clenbuterol-induced muscle growth. PLoS ONE, 14(6), 1-12. https://doi.org/10.1371/journal.pone.0218970

We previously identified PEPCK-M (encoded by the Pck2 gene) to be highly up-regulated in skeletal muscle of pigs treated with Ractopamine, an anabolic beta-adrenergic receptor agonist. To determine whether PEPCK-M had a causative role in modulating t... Read More about Effect of adeno-associated virus (AAV)-mediated overexpression of PEPCK-M (Pck2) on Clenbuterol-induced muscle growth.

The association between faecal host DNA or faecal calprotectin and feed efficiency in pigs fed yeast-enriched protein concentrate (2019)
Journal Article
Slinger, K. R., Stewart, A. H., Daniel, Z. C. T. R., Hall, H., Masey O’Neill, H. V., Bedford, M. R., …Brameld, J. M. (2019). The association between faecal host DNA or faecal calprotectin and feed efficiency in pigs fed yeast-enriched protein concentrate. Animal, 13(11), 2483-2491. https://doi.org/10.1017/s1751731119000818

Gut cell losses contribute to overall feed efficiency due to the energy requirement for cell replenishment. Intestinal epithelial cells are sloughed into the intestinal lumen as digesta passes through the gastrointestinal tract, where cells are degra... Read More about The association between faecal host DNA or faecal calprotectin and feed efficiency in pigs fed yeast-enriched protein concentrate.

The commercial pig as a model of spontaneously-occurring osteoarthritis (2019)
Journal Article
Macfadyen, M. A., Daniel, Z., Kelly, S., Parr, T., Brameld, J. M., Murton, A. J., & Jones, S. W. (2019). The commercial pig as a model of spontaneously-occurring osteoarthritis. BMC Musculoskeletal Disorders, 20, Article 70. https://doi.org/10.1186/s12891-019-2452-0

Background: Preclinical osteoarthritis models where damage occurs spontaneously may better reflect the initiation and development of human osteoarthritis. The aim was to assess the commercial pig as a model of spontaneous osteoarthritis development b... Read More about The commercial pig as a model of spontaneously-occurring osteoarthritis.

Productivity framework (2018)
Journal Article
Wagstaff, C., Kendrick, F., Dennis, C., Hollington, P., Hess, T., Brameld, J., …Mason, B. (2018). Productivity framework. Food Science and Technology -London-, 32(4), 56-61. https://doi.org/10.1002/fsat.3204_16.x

Researchers look at skills shortages and careers pathways in the agrifood industry. Sustaining the agriculture, food and drink industries requires human capital. Current estimates calculate that the agrifood supply chain employs approaching 4m people... Read More about Productivity framework.

The beta-adrenergic agonist, Ractopamine increases skeletal muscle expression of Asparagine Synthetase as part of an integrated stress response gene program (2018)
Journal Article
Brown, D., Ryan, K., Daniel, Z., Mareko, M., Talbot, R., Moreton, J., …Brameld, J. M. (2018). The beta-adrenergic agonist, Ractopamine increases skeletal muscle expression of Asparagine Synthetase as part of an integrated stress response gene program. Scientific Reports, 8, Article 15915. https://doi.org/10.1038/s41598-018-34315-9

Synthetic beta-adrenergic agonists (BA) have broad biomedical and agricultural application for increasing lean body mass, yet a poor understanding of the biology underpinning these agents is limiting further drug discovery potential. Growing female p... Read More about The beta-adrenergic agonist, Ractopamine increases skeletal muscle expression of Asparagine Synthetase as part of an integrated stress response gene program.

Effect of sodium 4-phenylbutyrate on clenbuterol-mediated muscle growth (2018)
Journal Article
Brown, D. M., Jones, S., Daniel, Z. C., Brearley, M. C., Lewis, J. E., Ebling, F. J., …Brameld, J. M. (2018). Effect of sodium 4-phenylbutyrate on clenbuterol-mediated muscle growth. PLoS ONE, 13(7), Article e0201481. https://doi.org/10.1371/journal.pone.0201481

Previously, we highlighted induction of an integrated stress response (ISR) gene program in skeletal muscle of pigs treated with a beta-adrenergic agonist. Hence we tested the hypothesis that the ER-stress inhibitor, sodium 4-phenylbutyrate (PBA), wo... Read More about Effect of sodium 4-phenylbutyrate on clenbuterol-mediated muscle growth.

Correction: Effects of insulin like growth factors on early embryonic chick limb myogenesis (2017)
Journal Article
Mohammed, R. H., Anderton, H., Brameld, J. M., & Sweetman, D. (2017). Correction: Effects of insulin like growth factors on early embryonic chick limb myogenesis. PLoS ONE, 12(12), https://doi.org/10.1371/journal.pone.0189395

© 2017 Mohammed et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are c... Read More about Correction: Effects of insulin like growth factors on early embryonic chick limb myogenesis.

Effects of insulin like growth factors on early embryonic chick limb myogenesis (2017)
Journal Article
Mohammed, R. H., Anderton, H., Brameld, J. M., & Sweetman, D. (2017). Effects of insulin like growth factors on early embryonic chick limb myogenesis. PLoS ONE, 12(10), Article e0185775. https://doi.org/10.1371/journal.pone.0185775

Limb muscles derive from pax3 expressing precursor cells that migrate from the hypaxial somite into the developing limb bud. Once there they begin to differentiate and express muscle determination genes such as MyoD. This process is regulated by a co... Read More about Effects of insulin like growth factors on early embryonic chick limb myogenesis.

Correction: Hypothalamic over-expression of VGF in the Siberian hamster increases energy expenditure and reduces body weight gain (PLoS ONE 12:2: (e0172724) DOI: 10.1371/journal.pone.0172724) (2017)
Journal Article
Lewis, J. E., Brameld, J. M., Hill, P., Cocco, C., Noli, B., Ferri, G. L., …Jethwa, P. H. (2017). Correction: Hypothalamic over-expression of VGF in the Siberian hamster increases energy expenditure and reduces body weight gain (PLoS ONE 12:2: (e0172724) DOI: 10.1371/journal.pone.0172724). PLoS ONE, 12(7), https://doi.org/10.1371/journal.pone.0182594

© 2017 Lewis et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are cred... Read More about Correction: Hypothalamic over-expression of VGF in the Siberian hamster increases energy expenditure and reduces body weight gain (PLoS ONE 12:2: (e0172724) DOI: 10.1371/journal.pone.0172724).

Gene expression of inflammatory markers in adipose tissue between obese women with polycystic ovary and normal obese women (2017)
Journal Article
Alshammari, G., Khan, R., Brameld, J. M., Amer, S. A., & Lomax, M. A. (2017). Gene expression of inflammatory markers in adipose tissue between obese women with polycystic ovary and normal obese women. European Review for Medical and Pharmacological Sciences, 21(5),

OBJECTIVE: The pathogenesis of polycystic ovary syndrome (PCOS), a common endocrine disease and metabolic disturbance, is still unknown. The aim of the study was to investigate whether patients with PCOS display increased expression of inflammatory m... Read More about Gene expression of inflammatory markers in adipose tissue between obese women with polycystic ovary and normal obese women.

Hypothalamic over-expression of VGF in the Siberian hamster increases energy expenditure and reduces body weight gain (2017)
Journal Article
Lewis, J. E., Brameld, J. M., Hill, P., Cocco, C., Noli, B., Ferri, G., …Jethwa, P. (2017). Hypothalamic over-expression of VGF in the Siberian hamster increases energy expenditure and reduces body weight gain. PLoS ONE, 12, Article e01727. https://doi.org/10.1371/journal.pone.0172724

VGF (non-acronymic) was first highlighted to have a role in energy homeostasis through experiments involving dietary manipulation in mice. Fasting increased VGF mRNA in the Arc and levels were subsequently reduced upon refeeding. This anabolic role f... Read More about Hypothalamic over-expression of VGF in the Siberian hamster increases energy expenditure and reduces body weight gain.

Mitochondrial phosphoenolpyruvate carboxykinase (PEPCK-M) and serine biosynthetic pathway genes are co-ordinately increased during anabolic agent-induced skeletal muscle growth (2016)
Journal Article
Brown, D. M., Williams, H., Ryan, K., Wilson, T., Daniel, Z. C., Mareko, M. H. D., …Parr, T. (2016). Mitochondrial phosphoenolpyruvate carboxykinase (PEPCK-M) and serine biosynthetic pathway genes are co-ordinately increased during anabolic agent-induced skeletal muscle growth. Scientific Reports, 6(1), https://doi.org/10.1038/srep28693

We aimed to identify novel molecular mechanisms for muscle growth during administration of anabolic agents. Growing pigs (Duroc/(Landrace/Large-White)) were administered Ractopamine (a beta-adrenergic agonist; BA; 20 ppm in feed) or Reporcin (recombi... Read More about Mitochondrial phosphoenolpyruvate carboxykinase (PEPCK-M) and serine biosynthetic pathway genes are co-ordinately increased during anabolic agent-induced skeletal muscle growth.

The impact of growth promoters on muscle growth and the potential consequences for meat quality (2016)
Journal Article
Parr, T., Mareko, M. H., Ryan, K. J., Hemmings, K. M., Brown, D. M., & Brameld, J. M. (2016). The impact of growth promoters on muscle growth and the potential consequences for meat quality. Meat Science, 120, 93-99. doi:10.1016/j.meatsci.2016.04.022

To meet the demands of increased global meat consumption, animal production systems will have to become more efficient, or at least maintain the current efficiency utilizing feed ingredients that are not also used for human consumption. Use of growth... Read More about The impact of growth promoters on muscle growth and the potential consequences for meat quality.

Improving efficiency in meat production (2016)
Journal Article
Brameld, J. M., & Parr, T. (in press). Improving efficiency in meat production. Proceedings of the Nutrition Society, https://doi.org/10.1017/S0029665116000161

Selective breeding and improved nutritional management over the past 20–30 years has resulted in dramatic improvements in growth efficiency for pigs and poultry, particularly lean tissue growth. However, this has been achieved using high-quality feed... Read More about Improving efficiency in meat production.

Thyroid hormone and vitamin D regulate VGF expression and promoter activity (2016)
Journal Article
Lewis, J. E., Brameld, J. M., Hill, P., Wilson, D., Barrett, P., Ebling, F. J., & Jethwa, P. H. (2016). Thyroid hormone and vitamin D regulate VGF expression and promoter activity. Journal of Molecular Endocrinology, 56(2), 123-134. https://doi.org/10.1530/JME-15-0224

The Siberian hamster (Phodopus sungorus) survives winter by decreasing food intake and catabolizing abdominal fat reserves, resulting in a sustained, profound loss of body weight. Hypothalamic tanycytes are pivotal for this process. In these cells, s... Read More about Thyroid hormone and vitamin D regulate VGF expression and promoter activity.

The use of viral 2A sequence for the simultaneous over-expression of both the vgf gene and enhanced green fluorescent protein eGFP (2015)
Journal Article
Lewis, J. E., Brameld, J. M., Hill, P., Barrett, P., Ebling, F. J., & Jethwa, P. (in press). The use of viral 2A sequence for the simultaneous over-expression of both the vgf gene and enhanced green fluorescent protein eGFP. Journal of Neuroscience Methods, 256, https://doi.org/10.1016/j.jneumeth.2015.08.013

Introduction: The viral 2A sequence has become an attractive alternative to the traditional internal ribosomal entry site (IRES) for simultaneous over-expression of two genes and in combination with recombinant adeno-associated viruses (rAAV) has bee... Read More about The use of viral 2A sequence for the simultaneous over-expression of both the vgf gene and enhanced green fluorescent protein eGFP.

Transcriptome analysis of mRNA and miRNA in skeletal muscle indicates an important network for differential Residual Feed Intake in pigs (2015)
Journal Article
Jing, L., Wu, H., Miao, Y., Cao, J., Brameld, J. M., Parr, T., & Zhao, S. (2015). Transcriptome analysis of mRNA and miRNA in skeletal muscle indicates an important network for differential Residual Feed Intake in pigs. Scientific Reports, 5, Article 11953. https://doi.org/10.1038/srep11953

Feed efficiency (FE) can be measured by feed conversion ratio (FCR) or residual feed intake (RFI). In this study, we measured the FE related phenotypes of 236 castrated purebred Yorkshire boars, and selected 10 extreme individuals with high and low R... Read More about Transcriptome analysis of mRNA and miRNA in skeletal muscle indicates an important network for differential Residual Feed Intake in pigs.

Neuroendocrine role for VGF (2015)
Journal Article
Lewis, J. E., Brameld, J. M., & Jethwa, P. H. (2015). Neuroendocrine role for VGF. Frontiers in Endocrinology, 6, doi:10.3389/fendo.2015.00003

Expression of the myosin heavy chain IIB gene in porcine skeletal muscle: the role of the CArG-box promoter response element (2014)
Journal Article
Brown, D. M., Brameld, J. M., & Parr, T. (2014). Expression of the myosin heavy chain IIB gene in porcine skeletal muscle: the role of the CArG-box promoter response element. PLoS ONE, 9(12), Article e114365. https://doi.org/10.1371/journal.pone.0114365

Due to its similarity to humans, the pig is increasingly being considered as a good animal model for studying a range of human diseases. Despite their physiological similarities, differential expression of the myosin heavy chain (MyHC) IIB gene (MYH4... Read More about Expression of the myosin heavy chain IIB gene in porcine skeletal muscle: the role of the CArG-box promoter response element.