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Phage control of Campylobacter jejuni in chickens does not produce collateral effects on the gut microbiota (2019)
Journal Article
Richards, P. J., Connerton, P. L., & Connerton, I. F. (2019). Phage control of Campylobacter jejuni in chickens does not produce collateral effects on the gut microbiota. Frontiers in Microbiology, 10, Article 476. https://doi.org/10.3389/fmicb.2019.00476

Bacteriophage biocontrol to reduce Campylobacter jejuni levels in chickens can reduce human exposure and disease acquired through the consumption of contaminated poultry products.Investigating changes in the chicken microbiota during phage treatment... Read More about Phage control of Campylobacter jejuni in chickens does not produce collateral effects on the gut microbiota.

FlhF(T368A) modulates motility in the bacteriophage carrier state of Campylobacter jejuni (2018)
Journal Article
Liang, L., & Connerton, I. F. (2018). FlhF(T368A) modulates motility in the bacteriophage carrier state of Campylobacter jejuni. Molecular Microbiology, 110(4), 616-633. https://doi.org/10.1111/mmi.14120

© 2018 The Authors. Molecular Microbiology Published by John Wiley & Sons Ltd. The carrier state is an alternative bacteriophage life cycle by which virulent bacteriophage can persist in association with host bacteria. Campylobacter jejuni carrier... Read More about FlhF(T368A) modulates motility in the bacteriophage carrier state of Campylobacter jejuni.

Bacteriophage ZCKP1: a potential treatment for Klebsiella pneumoniae isolated from diabetic foot patients (2018)
Journal Article
Taha, O. A., Connerton, P., Connerton, I., & El-Shibiny, A. (2018). Bacteriophage ZCKP1: a potential treatment for Klebsiella pneumoniae isolated from diabetic foot patients. Frontiers in Microbiology, 9, Article 2127. https://doi.org/10.3389/fmicb.2018.02127

The recorded growth in infection by multidrug resistant bacteria necessitates prompt efforts towards developing alternatives to antibiotics, such as bacteriophage therapy. Immuno-compromised patients with diabetes mellitus are particularly prone to f... Read More about Bacteriophage ZCKP1: a potential treatment for Klebsiella pneumoniae isolated from diabetic foot patients.

The effect of the timing of exposure to Campylobacter jejuni on the gut microbiome and inflammatory responses of broiler chickens (2018)
Journal Article
Connerton, P. L., Richards, P. J., Lafontaine, G. M., O'Kane, P. M., Ghaffar, N. M., Cummings, N. J., …Connerton, I. F. (in press). The effect of the timing of exposure to Campylobacter jejuni on the gut microbiome and inflammatory responses of broiler chickens. Microbiome, 6, Article 88. https://doi.org/10.1186/s40168-018-0477-5

Background Campylobacters are an unwelcome member of the poultry gut microbiota in terms of food safety. The objective of this study was to compare the microbiota, inflammatory responses, and zootechnical parameters of broiler chickens not exposed t... Read More about The effect of the timing of exposure to Campylobacter jejuni on the gut microbiome and inflammatory responses of broiler chickens.

Characterisation of aerotolerant forms of a robust chicken colonizing Campylobacter coli (2017)
Journal Article
O'Kane, P. M., & Connerton, I. F. (in press). Characterisation of aerotolerant forms of a robust chicken colonizing Campylobacter coli. Frontiers in Microbiology, 8, https://doi.org/10.3389/fmicb.2017.00513

Campylobacter contaminated poultry meat is a major source of human foodborne illness. Campylobacter coli strain OR12 is a robust colonizer of chickens that was previously shown to outcompete and displace other Campylobacter strains from the chicken’s... Read More about Characterisation of aerotolerant forms of a robust chicken colonizing Campylobacter coli.

The minor flagellin of Campylobacter jejuni (FlaB) confers defensive properties against bacteriophage infection (2016)
Journal Article
Lis, L., & Connerton, I. F. (in press). The minor flagellin of Campylobacter jejuni (FlaB) confers defensive properties against bacteriophage infection. Frontiers in Microbiology, 7, https://doi.org/10.3389/fmicb.2016.01908

A screen of bacteriophages infecting a panel of Campylobacter jejuni PT14 gene knock-out mutants identified a role for the minor flagellin encoded by the flaB gene, in the defense of the host against CP8unalikevirus bacteriophage CP_F1 infection. Ina... Read More about The minor flagellin of Campylobacter jejuni (FlaB) confers defensive properties against bacteriophage infection.

Campylobacters and their bacteriophages from chicken liver: the prospect for phage biocontrol (2016)
Journal Article
Firlieyanti, A. S., Connerton, P. L., & Connerton, I. F. (2016). Campylobacters and their bacteriophages from chicken liver: the prospect for phage biocontrol. International Journal of Food Microbiology, 237, 121-127. https://doi.org/10.1016/j.ijfoodmicro.2016.08.026

Consumption of foods containing chicken liver has been associated with Campylobacter enteritis. Campylobacters can contaminate the surface of livers post-mortem but can also arise through systemic infection of colonising bacteria in live birds. The u... Read More about Campylobacters and their bacteriophages from chicken liver: the prospect for phage biocontrol.

The effect of thermal processing in oil on the macromolecular integrity and acrylamide formation from starch of three potato cultivars organically fertilized (2016)
Journal Article
Varzakas, T., Alghamdi, A., Alghamdi, H., Linforth, R. S., Dinu, V., Besong, T. D., …Israilides, C. (2016). The effect of thermal processing in oil on the macromolecular integrity and acrylamide formation from starch of three potato cultivars organically fertilized. Cogent Food and Agriculture, 2(1), https://doi.org/10.1080/23311932.2016.1180950

Starches from three organically produced cultivars of potato tuber (Lady Rosetta, Spunta and Voyager) have been studied in relation to (i) acrylamide production (ii) macromolecular integrity after frying with extra virgin olive oil, soybean oil and c... Read More about The effect of thermal processing in oil on the macromolecular integrity and acrylamide formation from starch of three potato cultivars organically fertilized.

The bacteriophage carrier state of Campylobacter jejuni features changes in host non-coding RNAs and the acquisition of new host-derived CRISPR spacer sequences (2016)
Journal Article
Hooton, S. P., Brathwaite, K. J., & Connerton, I. F. (2016). The bacteriophage carrier state of Campylobacter jejuni features changes in host non-coding RNAs and the acquisition of new host-derived CRISPR spacer sequences. Frontiers in Microbiology, 7(355), Article 355. https://doi.org/10.3389/fmicb.2016.00355

Incorporation of self-derived CRISPR DNA protospacers in Campylobacter jejuni PT14 occurs in the presence of bacteriophages encoding a CRISPR-like Cas4 protein. This phenomenon was evident in carrier state infections where both bacteriophages and ho... Read More about The bacteriophage carrier state of Campylobacter jejuni features changes in host non-coding RNAs and the acquisition of new host-derived CRISPR spacer sequences.

Pilot study of long-term anaesthesia in broiler chickens (2016)
Journal Article
O'Kane, P. M., Connerton, I. F., & White, K. L. (2016). Pilot study of long-term anaesthesia in broiler chickens. Veterinary Anaesthesia and Analgesia, 43(1), 72-75. https://doi.org/10.1111/vaa.12305

Objective: To provide stable anaesthesia of long duration in broiler chickens in order to perform a terminal caecal ligated loop procedure. Study design: Prospective experimental study. Animals: Seven clinically healthy broiler chickens (Gallus... Read More about Pilot study of long-term anaesthesia in broiler chickens.

Phase variation of a Type IIG restriction-modification enzyme alters site-specific methylation patterns and gene expression in Campylobacter jejuni strain NCTC11168 (2016)
Journal Article
Anjum, A., Kelly, B., Aidley, J. B., Connerton, P. L., Cummings, N. J., Parkhill, J., …Bayliss, C. D. (2016). Phase variation of a Type IIG restriction-modification enzyme alters site-specific methylation patterns and gene expression in Campylobacter jejuni strain NCTC11168. Nucleic Acids Research, https://doi.org/10.1093/nar/gkw019

Phase-variable restriction-modification systems are a feature of a diverse range of bacterial species. Stochastic, reversible switches in expression of the methyltransferase produces variation in methylation of specific sequences. Phase-variable meth... Read More about Phase variation of a Type IIG restriction-modification enzyme alters site-specific methylation patterns and gene expression in Campylobacter jejuni strain NCTC11168.

Filamentation of Campylobacter in broth cultures (2015)
Journal Article
Ghaffar, N. M., Connerton, P. L., & Connerton, I. F. (2015). Filamentation of Campylobacter in broth cultures. Frontiers in Microbiology, 6(657), https://doi.org/10.3389/fmicb.2015.00657

The transition from rod to filamentous cell morphology has been identified as a response to stressful conditions in many bacterial species and has been ascribed to confer certain survival advantages. Filamentation of Campylobacter jejuni was demonstr... Read More about Filamentation of Campylobacter in broth cultures.

Host adaption to the bacteriophage carrier state of Campylobacter jejuni (2015)
Journal Article
Brathwaite, K. J., Siringan, P., Connerton, P. L., & Connerton, I. F. (2015). Host adaption to the bacteriophage carrier state of Campylobacter jejuni. Research in Microbiology, 166(6), 504-515. https://doi.org/10.1016/j.resmic.2015.05.003

The carrier state of the foodborne pathogen Campylobacter jejuni represents an alternative life cycle whereby virulent bacteriophage can persistent in association with host bacteria without commitment to lysogeny. Host bacteria exhibit significant ph... Read More about Host adaption to the bacteriophage carrier state of Campylobacter jejuni.

Xylan degrading enzymes from fungal sources (2015)
Journal Article
Kirikyali, N., & Connerton, I. F. (2015). Xylan degrading enzymes from fungal sources. Journal of Biocatalysis and Biotransformation, 4(1), Article 118. https://doi.org/10.4172/10.4172/jpe.1000118

Fungi have the ability to degrade xylan as the major component of plant cell wall hemicellulose. Fungi have evolved batteries of xylanolytic enzymes that concertedly act to depolymerise xylan backbones decorated with variable carbohydrate branches. A... Read More about Xylan degrading enzymes from fungal sources.

Campylobacter jejuni acquire new host-derived CRISPR spacers when in association with bacteriophages harboring a CRISPR-like Cas4 protein (2015)
Journal Article
Hooton, S. P., & Connerton, I. F. (2015). Campylobacter jejuni acquire new host-derived CRISPR spacers when in association with bacteriophages harboring a CRISPR-like Cas4 protein. Frontiers in Microbiology, 5(744), https://doi.org/10.3389/fmicb.2014.00744

Campylobacter jejuni is a worldwide cause of human diarrhoeal disease. Clustered Repetitively Interspaced Palindromic Repeats (CRISPRs) and associated proteins allow Bacteria and Archaea to evade bacteriophage and plasmid infection. Type II CRISPR sy... Read More about Campylobacter jejuni acquire new host-derived CRISPR spacers when in association with bacteriophages harboring a CRISPR-like Cas4 protein.

Characterisation of a recombinant ?-xylosidase (xylA) from Aspergillus oryzae expressed in Pichia pastoris (2014)
Journal Article
Kirikyali, N., Wood, J., & Connerton, I. F. (2014). Characterisation of a recombinant ?-xylosidase (xylA) from Aspergillus oryzae expressed in Pichia pastoris. AMB Express, 4(68), https://doi.org/10.1186/s13568-014-0068-1

β-xylosidases catalyse the hydrolysis of short chain xylooligosaccharides from their non-reducing ends into xylose. In this study we report the heterologous expression of Aspergillus oryzae β-xylosidase (XylA) in Pichia pastoris under the control of... Read More about Characterisation of a recombinant ?-xylosidase (xylA) from Aspergillus oryzae expressed in Pichia pastoris.

The complete plasmid sequences of Salmonella enterica serovar Typhimurium U288 (2014)
Journal Article
Hooton, S. P., Timms, A. R., Cummings, N. J., Moreton, J., Wilson, R., & Connerton, I. F. (2014). The complete plasmid sequences of Salmonella enterica serovar Typhimurium U288. Plasmid, 76, 32-39. https://doi.org/10.1016/j.plasmid.2014.08.002

Salmonella enterica Serovar Typhimurium U288 is an emerging pathogen of pigs. The strain contains three plasmids of diverse origin that encode traits that are of concern for food security and safety, these include antibiotic resistant determinants, a... Read More about The complete plasmid sequences of Salmonella enterica serovar Typhimurium U288.

Food safety organisations in Saudi Arabia – organisational, historical and future analysis (2014)
Journal Article
Al-Mutairi, S., Connerton, I., & Dingwall, R. (2015). Food safety organisations in Saudi Arabia – organisational, historical and future analysis. Food Control, 47, 478-486. https://doi.org/10.1016/j.foodcont.2014.07.047

Food safety organisations around the world are increasingly seeking to develop public policies to protect society, improve the economy, and ensure food safety and quality, by reducing the risks of food-borne illnesses, commercial adulteration, and fo... Read More about Food safety organisations in Saudi Arabia – organisational, historical and future analysis.

Alternative bacteriophage life cycles: the carrier state of Campylobacter jejuni (2014)
Journal Article
Siringan, P., Connerton, P. L., Cummmings, N. J., & Connerton, I. F. (2014). Alternative bacteriophage life cycles: the carrier state of Campylobacter jejuni. Open Biology, 4(3), Article 130200. https://doi.org/10.1098/rsob.130200

Members of the genus Campylobacter are frequently responsible for human enteric disease, often through consumption of contaminated poultry products. Bacteriophages are viruses that have the potential to control pathogenic bacteria, but understanding... Read More about Alternative bacteriophage life cycles: the carrier state of Campylobacter jejuni.