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Component analysis of nutritionally rich chloroplasts: recovery from conventional and unconventional green plant species (2017)
Journal Article
Gedi, M. A., Briars, R., Yuseli, F., Zainol, N., Darwish, R., Salter, A. M., & Gray, D. A. (2017). Component analysis of nutritionally rich chloroplasts: recovery from conventional and unconventional green plant species. Journal of Food Science and Technology, 54(9), 2746-2757. https://doi.org/10.1007/s13197-017-2711-8

A study of the literature indicates that chloroplasts synthesise a range of molecules, many of which have nutritional value for humans, but as yet no one has established the nutritional credentials of chloroplasts recovered from plant cells. Chloropl... Read More about Component analysis of nutritionally rich chloroplasts: recovery from conventional and unconventional green plant species.

Influence of pecan nut pretreatment on the physical quality of oil bodies (2017)
Journal Article
Zhang, P., Di Bari, V., Briars, R., Taher, Z. M., Yuan, J., Liu, G., & Gray, D. A. (2017). Influence of pecan nut pretreatment on the physical quality of oil bodies. Journal of Food Quality, 2017(38641), Article 3864126. https://doi.org/10.1155/2017/3864126

A supply of pure, intact oil bodies is essential for carrying out morphological and biochemical studies of these plant organelles, and exploring their application. Preparation requires a carefully controlled breakage of plant cells, followed by separ... Read More about Influence of pecan nut pretreatment on the physical quality of oil bodies.

The role of plant cell wall encapsulation and porosity in regulating lipolysis during the digestion of almond seeds (2015)
Journal Article
Grundy, M. M., Carrière, F., Mackie, A. R., Gray, D. A., Butterworth, P. J., & Ellis, P. R. (2016). The role of plant cell wall encapsulation and porosity in regulating lipolysis during the digestion of almond seeds. Food and Function, 7(1), 69-78. https://doi.org/10.1039/c5fo00758e

Previous studies have provided evidence that the physical encapsulation of intracellular nutrients by cell walls of plant foods (i.e. dietary fibre) plays a predominant role in influencing macronutrient bioaccessibility (release) from plant foods dur... Read More about The role of plant cell wall encapsulation and porosity in regulating lipolysis during the digestion of almond seeds.

Impact of extraneous proteins on the gastrointestinal fate of sunflower seed (Helianthus annuus) oil bodies: a simulated gastrointestinal tract study (2014)
Journal Article
Makkhun, S., Khosla, A., Foster, T., McClements, D. J., Grundy, M. M., & Gray, D. (2015). Impact of extraneous proteins on the gastrointestinal fate of sunflower seed (Helianthus annuus) oil bodies: a simulated gastrointestinal tract study. Food and Function, 6(1), doi:10.1039/C4FO00422A

In this study, we examined the physicochemical nature of sunflower seed oil bodies (in the absence and presence of added protein) exposed to gastrointestinal conditions in vitro: crude oil bodies (COB); washed oil bodies (WOB); whey protein isolate-e... Read More about Impact of extraneous proteins on the gastrointestinal fate of sunflower seed (Helianthus annuus) oil bodies: a simulated gastrointestinal tract study.

Composition and properties of the surface of oil bodies recovered from Echium Plantagineum (2013)
Journal Article
Payne, G., Lad, M., Foster, T., Khosla, A., & Gray, D. (2014). Composition and properties of the surface of oil bodies recovered from Echium Plantagineum. Colloids and Surfaces B: Biointerfaces, 116, 88-92. doi:10.1016/j.colsurfb.2013.11.043

Neutral-lipids within oilseeds are most commonly stored in oil bodies, small spherical organelles with oleosin proteins inserted through a phospholipid monolayer. Oil bodies extracted from Echium plantagineum are highly enriched in polyunsaturated fa... Read More about Composition and properties of the surface of oil bodies recovered from Echium Plantagineum.

Entrapment of a volatile lipophilic aroma compound (D-limonene) in spray dried water-washed oil bodies naturally derived from sun?ower seeds (Helianthus annus) (2013)
Journal Article
Fisk, I. D., Linforth, R. S., Trophardy, G., & Gray, D. A. (2013). Entrapment of a volatile lipophilic aroma compound (D-limonene) in spray dried water-washed oil bodies naturally derived from sun?ower seeds (Helianthus annus). Food Research International, 54(1), https://doi.org/10.1016/j.foodres.2013.08.024

Oil bodies are natural emulsions that can be extracted from oil seeds and have previously been shown to be stable after spray drying. The aim of the study was to evaluate for the first time if spray dried water-washed oil bodies are an effective car... Read More about Entrapment of a volatile lipophilic aroma compound (D-limonene) in spray dried water-washed oil bodies naturally derived from sun?ower seeds (Helianthus annus).

The lipidome and proteome of oil bodies from Helianthus annuus (common sunflower) (2013)
Journal Article
Furse, S., Liddell, S., Ortori, C. A., Williams, H., Neylon, D. C., Scott, D. J., …Gray, D. A. (2013). The lipidome and proteome of oil bodies from Helianthus annuus (common sunflower). Journal of Chemical Biology, 6(2), 63-76. https://doi.org/10.1007/s12154-012-0090-1

In this paper we report the molecular profiling, lipidome and proteome, of the plant organelle known as an oil body (OB). The OB is remarkable in that it is able to perform its biological role (storage of triglycerides) whilst resisting the physical... Read More about The lipidome and proteome of oil bodies from Helianthus annuus (common sunflower).

Isolation and characterization of oil bodies from Oryza sativa bran and studies of their physical properties (2012)
Journal Article
Nantiyakul, N., Furse, S., Fisk, I. D., Tucker, G., & Gray, D. A. (2013). Isolation and characterization of oil bodies from Oryza sativa bran and studies of their physical properties. Journal of Cereal Science, 57(1), 141-145. https://doi.org/10.1016/j.jcs.2012.11.002

In this paper, we demonstrate a novel and environmentally-conscious approach to the isolation of oil bodies (OBs) from the bran arising from the milling of Oryza sativa (Basmati rice). We have used several physical techniques to determine the effect... Read More about Isolation and characterization of oil bodies from Oryza sativa bran and studies of their physical properties.

Phytochemical Composition of Oryza sativa (Rice) Bran Oil Bodies in Crude and Purified Isolates (2012)
Journal Article
Nantiyakul, N., Furse, S., Fisk, I., Foster, T. J., Tucker, G., & Gray, D. A. (2012). Phytochemical Composition of Oryza sativa (Rice) Bran Oil Bodies in Crude and Purified Isolates. Journal of the American Oil Chemists' Society, 89(10), 1867-1872. https://doi.org/10.1007/s11746-012-2078-y

We describe a procedure for isolating and purifying oil bodies (OBs) from Oryza sativa bran, and present evidence that strongly suggests a physical association between the OB organelles and several antioxidant phytochemicals (c-oryzanol and several t... Read More about Phytochemical Composition of Oryza sativa (Rice) Bran Oil Bodies in Crude and Purified Isolates.

Soybean (Glycine max) Oil Bodies and Their Associated Phytochemicals (2011)
Journal Article
Fisk, I. D., & Gray, D. A. (2011). Soybean (Glycine max) Oil Bodies and Their Associated Phytochemicals. Journal of Food Science, 76(9), C1349-C1354. https://doi.org/10.1111/j.1750-3841.2011.02428.x

Abstract:? Soybean oil bodies were isolated from 3 cultivars (Ustie, K98, and Elena) and the occurrence of 2 classes of phytochemicals (tocopherol isoforms and isoflavones) and strength of their association with isolated oil bodies was evaluated. Toc... Read More about Soybean (Glycine max) Oil Bodies and Their Associated Phytochemicals.

Aroma encapsulation and aroma delivery by oil body suspensions derived from sunflower seeds (Helianthus annus) (2011)
Journal Article
Fisk, I. D., Linforth, R. S., Taylor, A. J., & Gray, D. A. (2011). Aroma encapsulation and aroma delivery by oil body suspensions derived from sunflower seeds (Helianthus annus). European Food Research and Technology, 232(5), https://doi.org/10.1007/s00217-011-1459-z

Oil bodies are small discrete cell organelles that can be found within oilseeds. Oil bodies have been investigated previously as a potential technology platform for use within the food industry, offering stable, antioxidant-enriched lipid-delivery... Read More about Aroma encapsulation and aroma delivery by oil body suspensions derived from sunflower seeds (Helianthus annus).

In vitro assessment of the bioaccessibility of tocopherol and fatty acids from sunflower seed oil bodies (2009)
Journal Article
White, D. A., Fisk, I. D., Makkhun, S., & Gray, D. A. (2009). In vitro assessment of the bioaccessibility of tocopherol and fatty acids from sunflower seed oil bodies. Journal of Agricultural and Food Chemistry, 57(13), 5720-5726. https://doi.org/10.1021/jf9003412

The in vitro digestibility (proteolytic and lipolytic) and bioaccessibility of nutritionally important compounds (α-tocopherol and fatty acids) have been studied for natural sunflower (Helianthus annuus) oil body suspensions in comparison to artifici... Read More about In vitro assessment of the bioaccessibility of tocopherol and fatty acids from sunflower seed oil bodies.

Gamma-irradiation as a method of microbiological control, and its impact on the oxidative labile lipid component of Cannabis sativa and Helianthus annus (2008)
Journal Article
Fisk, I. D., Gkatzionis, K., Lad, M., Dodd, C. E., & Gray, D. A. (2009). Gamma-irradiation as a method of microbiological control, and its impact on the oxidative labile lipid component of Cannabis sativa and Helianthus annus. European Food Research and Technology, 228(4), 613-621. https://doi.org/10.1007/s00217-008-0970-3

The effect of irradiation (0-20 kGy) on hemp and sunflower seeds was assessed, with specific reference to the oxidatively labile lipid component (unsaturated fatty acids and tocochromanols). Total protein, lipid, and solids content of the seeds did n... Read More about Gamma-irradiation as a method of microbiological control, and its impact on the oxidative labile lipid component of Cannabis sativa and Helianthus annus.

Oxidative stability of sunflower oil bodies (2008)
Journal Article
Fisk, I. D., White, D. A., Lad, M., & Gray, D. A. (2008). Oxidative stability of sunflower oil bodies. European Journal of Lipid Science and Technology, 110(10), 962-968. https://doi.org/10.1002/ejlt.200800051

This study investigates the oxidative stability of sunflower oil body suspensions (10 wt-% lipid). Two washed suspensions of oil bodies were evaluated over 8 days at three temperatures (5, 25 and 45 °C) against three comparable sunflower oil emulsion... Read More about Oxidative stability of sunflower oil bodies.

Extraction and characterization of oil bodies from soy beans: A natural source of pre-emulsified soybean oil (2007)
Journal Article
Iwanaga, D., Gray, D. A., Fisk, I. D., Decker, E. A., Weiss, J., & McClements, D. J. (2007). Extraction and characterization of oil bodies from soy beans: A natural source of pre-emulsified soybean oil. Journal of Agricultural and Food Chemistry, 55(21), 8711-8716. https://doi.org/10.1021/jf071008w

Soybeans contain oil bodies that are coated by a layer of oleosin proteins. In nature, this protein coating protects the oil bodies from environmental stresses and may be utilized by food manufacturers for the same purpose. In this study, oil bodies... Read More about Extraction and characterization of oil bodies from soy beans: A natural source of pre-emulsified soybean oil.

Sunflower-seed oil body emulsions: Rheology and stability assessment of a natural emulsion (2007)
Journal Article
White, D. A., Fisk, I. D., Mitchell, J. R., Wolf, B., Hill, S. E., & Gray, D. A. (2008). Sunflower-seed oil body emulsions: Rheology and stability assessment of a natural emulsion. Food Hydrocolloids, 22(7), 1224-1232. https://doi.org/10.1016/j.foodhyd.2007.07.004

The viscoelastic characteristics of a purified oil body cream (67.6±0.7% lipid, 5.4±0.7% protein and 25.2±0.1% moisture) recovered from sunflower seeds (Helianthus annuus L.) have been determined. Moreover, the effects of pH (2-7) and CaCl2 concentra... Read More about Sunflower-seed oil body emulsions: Rheology and stability assessment of a natural emulsion.

Tocopherol: an intrinsic component of sunflower seed oil bodies (2006)
Journal Article
Fisk, I. D., White, D. A., Carvalho, A., & Gray, D. A. (2006). Tocopherol: an intrinsic component of sunflower seed oil bodies. Journal of the American Oil Chemists' Society, 83(4), (341-344). doi:10.1007/s11746-006-1210-2. ISSN 0003-021X

Oil bodies were removed from mature sunflower through wet grinding followed by filtration then centrifugation and recovered as the buoyant fraction. Washing this fraction with buffer (water?washed oil bodies, WWOB) or 9 M urea (urea?washed oil bodies... Read More about Tocopherol: an intrinsic component of sunflower seed oil bodies.

Characterisation of oat (Avena sativa L.) oil bodies and intrinsically associated E-vitamers (2005)
Journal Article
White, D. A., Fisk, I. D., & Gray, D. A. (2006). Characterisation of oat (Avena sativa L.) oil bodies and intrinsically associated E-vitamers. Journal of Cereal Science, 43(2), 244-249. https://doi.org/10.1016/j.jcs.2005.10.002

Transmission electron microscopy of sections of oat (Avena sativa L.) grain suggested that the highest concentrations oil bodies were in the aleurone and germ rather than the starchy endosperm. Oil bodies recovered from homogenized tissues by centrif... Read More about Characterisation of oat (Avena sativa L.) oil bodies and intrinsically associated E-vitamers.