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The butanol producing microbe Clostridium beijerinckii NCIMB 14988 manipulated using forward and reverse genetic tools (2018)
Journal Article
Little, G. T., Willson, B. J., Heap, J. T., Winzer, K., & Minton, N. P. (2018). The butanol producing microbe Clostridium beijerinckii NCIMB 14988 manipulated using forward and reverse genetic tools. Biotechnology Journal, doi:10.1002/biot.201700711. ISSN 1860-6768

The solventogenic anaerobe Clostridium beijerinckii has potential for use in the sustainable bioconversion of plant-derived carbohydrates into solvents, such as butanol or acetone. However, relatively few strains have been extensively characterised e... Read More about The butanol producing microbe Clostridium beijerinckii NCIMB 14988 manipulated using forward and reverse genetic tools.

Quantitative methods to monitor RNA biomarkers in myotonic dystrophy (2018)
Journal Article
Wojciechowska, M., Sobczak, K., Kozlowski, P., Sedehizadeh, S., Wojtkowiak-Szlachcic, A., Czubak, K., …Brook, J. D. (in press). Quantitative methods to monitor RNA biomarkers in myotonic dystrophy. Scientific Reports, 8, doi:10.1038/s41598-018-24156-x

Myotonic dystrophy type 1 (DM1) and type 2 (DM2) are human neuromuscular disorders associated with mutations of simple repetitive sequences in afected genes. The abnormal expansion of CTG repeats in the 3′-UTR of the DMPK gene elicits DM1, whereas el... Read More about Quantitative methods to monitor RNA biomarkers in myotonic dystrophy.

A transcription factor-based biosensor for detection of itaconic acid (2018)
Journal Article
Hanko, E. K., Minton, N. P., & Malys, N. (2018). A transcription factor-based biosensor for detection of itaconic acid. ACS Synthetic Biology, 7(5), (1436–1446). doi:10.1021/acssynbio.8b00057. ISSN 2161-5063

Itaconic acid is an important platform chemical that can easily be incorporated into polymers and has the potential to replace petrochemical-based acrylic or methacrylic acid. A number of microorganisms have been developed for the biosynthesis of ita... Read More about A transcription factor-based biosensor for detection of itaconic acid.

The inhibition of human lung fibroblast proliferation and differentiation by Gs-coupled receptors is not predicted by the magnitude of cAMP response (2018)
Journal Article
Roberts, M. J., Broome, R. E., Kent, T. C., Charlton, S. J., & Rosethorne, E. M. (2018). The inhibition of human lung fibroblast proliferation and differentiation by Gs-coupled receptors is not predicted by the magnitude of cAMP response. Respiratory Research, 19, doi:10.1186/s12931-018-0759-2

Background Idiopathic pulmonary fibrosis (IPF) is 22 a chronic and progressive fibrotic lung disease for which there is no cure. Current therapeutics are only able to slow disease progression, therefore there is a need to explore alternative, novel t... Read More about The inhibition of human lung fibroblast proliferation and differentiation by Gs-coupled receptors is not predicted by the magnitude of cAMP response.

Plasmodium APC3 mediates chromosome condensation and cytokinesis during atypical mitosis in male gametogenesis (2018)
Journal Article
Wall, R. J., Ferguson, D. J. P., Freville, A., Franke-Fayard, B., Brady, D., Zeeshan, M., …Tewari, R. (in press). Plasmodium APC3 mediates chromosome condensation and cytokinesis during atypical mitosis in male gametogenesis. Scientific Reports, 8(1), doi:10.1038/s41598-018-23871-9

The anaphase promoting complex/cyclosome (APC/C) is a highly conserved multi-subunit E3 ubiquitin ligase that controls mitotic division in eukaryotic cells by tagging cell cycle regulators for proteolysis. APC3 is a key component that contributes to... Read More about Plasmodium APC3 mediates chromosome condensation and cytokinesis during atypical mitosis in male gametogenesis.

Imaging the dynamics of transcription loops in living chromosomes (2018)
Journal Article
Morgan, G. T. (2018). Imaging the dynamics of transcription loops in living chromosomes. Chromosoma, 127(3), (361-374). doi:10.1007/s00412-018-0667-8. ISSN 0009-5915

When in the lampbrush configuration chromosomes display thousands of visible DNA loops that are transcribed at exceptionally high rates by RNA polymerase II (pol II). These transcription loops provide unique opportunities to investigate not only the... Read More about Imaging the dynamics of transcription loops in living chromosomes.

Lipophilicity predicts the ability of nonsulphonylurea drugs to block pancreatic beta-cell KATP channels and stimulate insulin secretion: statins as a test case (2018)
Journal Article
Real, J., Miranda, C., Olofsson, C. S., & Smith, P. A. (2018). Lipophilicity predicts the ability of nonsulphonylurea drugs to block pancreatic beta-cell KATP channels and stimulate insulin secretion: statins as a test case. doi:10.1002/edm2.17

Aims KATP ion channels play a key role in glucose‐stimulated insulin secretion. However, many drugs block KATP as “off targets” leading to hyperinsulinaemia and hypoglycaemia. As such drugs are often lipophilic, the aim was to examine the relation... Read More about Lipophilicity predicts the ability of nonsulphonylurea drugs to block pancreatic beta-cell KATP channels and stimulate insulin secretion: statins as a test case.

Developmental functions of the dynamic DNA methylome and hydroxymethylome in the mouse and zebrafish: similarities and differences (2018)
Journal Article
Jessop, P., Ruzov, A., & Gering, M. (2018). Developmental functions of the dynamic DNA methylome and hydroxymethylome in the mouse and zebrafish: similarities and differences. Frontiers in Cell and Developmental Biology, 6, doi:10.3389/fcell.2018.00027

5-methylcytosine (5mC) is the best understood DNA modification and is generally believed to be associated with repression of gene expression. Over the last decade, sequentially oxidized forms of 5mC (oxi-mCs) have been discovered within the genomes o... Read More about Developmental functions of the dynamic DNA methylome and hydroxymethylome in the mouse and zebrafish: similarities and differences.

Synthesis and evaluation of the first fluorescent antagonists of the human P2Y2 receptor based on AR-C118925 (2018)
Journal Article
Conroy, S., Kindon, N., Glenn, J., Stoddart, L. A., Lewis, R. J., Hill, S. J., …Stocks, M. J. (2018). Synthesis and evaluation of the first fluorescent antagonists of the human P2Y2 receptor based on AR-C118925. Journal of Medicinal Chemistry, 61(7), doi:10.1021/acs.jmedchem.8b00139

The human P2Y2 receptor (hP2Y2R) is a G protein-coupled receptor that shows promise as a therapeutic target for many important conditions including anti-metastatic cancer therapy and more recently for the treatment of idiopathic pulmonary fibrosis. A... Read More about Synthesis and evaluation of the first fluorescent antagonists of the human P2Y2 receptor based on AR-C118925.

Biased agonism in drug discovery: is it too soon to choose a path? (2018)
Journal Article
Michel, M. C., & Charlton, S. J. (2018). Biased agonism in drug discovery: is it too soon to choose a path?. Molecular Pharmacology, 93(4), doi:10.1124/mol.117.110890

A single receptor can activate multiple signaling pathways that have distinct or even opposite effects on cell function. Biased agonists stabilize receptor conformations preferentially stimulating one of these pathways, and therefore allow a more tar... Read More about Biased agonism in drug discovery: is it too soon to choose a path?.

Dynamic metabolic patterns tracking neurodegeneration and gliosis following 26S proteasome dysfunction in mouse forebrain neurons (2018)
Journal Article
Geiszler, P. C., Ugun-Klusek, A., Lawler, K., Pardon, M., Yuchun, D., Bai, L., …Bedford, L. (in press). Dynamic metabolic patterns tracking neurodegeneration and gliosis following 26S proteasome dysfunction in mouse forebrain neurons. Scientific Reports, 8, doi:10.1038/s41598-018-23155-2

Metabolite profiling is an important tool that may better capture the multiple features of neurodegeneration. With the considerable parallels between mouse and human metabolism, the use of metabolomics in mouse models with neurodegenerative pathology... Read More about Dynamic metabolic patterns tracking neurodegeneration and gliosis following 26S proteasome dysfunction in mouse forebrain neurons.

VEGFR2 promotes central endothelial activation and the spread of pain in inflammatory arthritis (2018)
Journal Article
Beazley-Long, N., Moss, C. E., Ashby, W. R., Bestall, S. M., Almahasneh, F., Durrant, A. M., …Donaldson, L. F. (2018). VEGFR2 promotes central endothelial activation and the spread of pain in inflammatory arthritis. Brain, Behavior, and Immunity, 74, 49-67. https://doi.org/10.1016/j.bbi.2018.03.012

Chronic pain can develop in response to conditions such as inflammatory arthritis. The central mechanisms underlying the development and maintenance of chronic pain in humans are not well elucidated although there is evidence for a role of microglia... Read More about VEGFR2 promotes central endothelial activation and the spread of pain in inflammatory arthritis.

Quantitative isotope-dilution high-resolution-mass-apectrometry analysis of multiple intracellular metabolites in Clostridium autoethanogenum with uniformly 13C-labeled standards derived from Spirulina (2018)
Journal Article
Schatschneider, S., Abdelrazig, S. M., Safo, L., Henstra, A. M., Millat, T., Kim, D., …Barrett, D. A. (2018). Quantitative isotope-dilution high-resolution-mass-apectrometry analysis of multiple intracellular metabolites in Clostridium autoethanogenum with uniformly 13C-labeled standards derived from Spirulina. Analytical Chemistry, 90(7), doi:10.1021/acs.analchem.7b04758

We have investigated the applicability of commercially available lyophilized spirulina (Arthrospira platensis), a microorganism uniformly labeled with 13C, as a readily accessible source of multiple 13C-labeled metabolites suitable as internal standa... Read More about Quantitative isotope-dilution high-resolution-mass-apectrometry analysis of multiple intracellular metabolites in Clostridium autoethanogenum with uniformly 13C-labeled standards derived from Spirulina.

Dispersion of relative importance values contributes to the ranking uncertainty: sensitivity analysis of Multiple Criteria Decision-Making methods (2018)
Journal Article
Maliene, V., Dixon-Gough, R., & Malys, N. (2018). Dispersion of relative importance values contributes to the ranking uncertainty: sensitivity analysis of Multiple Criteria Decision-Making methods. Applied Soft Computing, 67, doi:10.1016/j.asoc.2018.03.003

Multiple Criteria Decision-Making (MCDM) methods are widely used in research and industrial applications. These methods rely heavily on expert perceptions and are often sensitive to the assumptions made. The reliability and robustness of MCDM analysi... Read More about Dispersion of relative importance values contributes to the ranking uncertainty: sensitivity analysis of Multiple Criteria Decision-Making methods.

Functional identification of the prnABCD operon and its regulation in Serratia plymuthica (2018)
Journal Article
Liu, X., Yu, X., Yang, Y., Heeb, S., Gao, S., Chan, K. G., …Gao, K. (in press). Functional identification of the prnABCD operon and its regulation in Serratia plymuthica. Applied Microbiology and Biotechnology, doi:10.1007/s00253-018-8857-0

The antibiotic pyrrolnitrin (PRN) is a tryptophan-derived secondary metabolite that plays an important role in the biocontrol of plant diseases due to its broad-spectrum of antimicrobial activities. The PRN biosynthetic gene cluster remains to be cha... Read More about Functional identification of the prnABCD operon and its regulation in Serratia plymuthica.

2-Tridecanone impacts surface-associated bacterial behaviours and hinders plant-bacteria interactions (2018)
Journal Article
López-Lara, I. M., Nogales, J., Pech-Canul, A., Calatrava-Morales, N., Bernabéu-Roda, L. M., Durán, P., …Soto, M. J. (2018). 2-Tridecanone impacts surface-associated bacterial behaviours and hinders plant-bacteria interactions. Environmental Microbiology, 20(6), 2049-2065. doi:10.1111/1462-2920.14083

Surface motility and biofilm formation are behaviours which enable bacteria to infect their hosts and are controlled by different chemical signals. In the plant symbiotic alpha‐proteobacterium Sinorhizobium meliloti, the lack of long‐chain fatty acyl... Read More about 2-Tridecanone impacts surface-associated bacterial behaviours and hinders plant-bacteria interactions.

Understanding hepatitis C intervention success: qualitative findings from the HepCATT study (2018)
Journal Article
Harris, M., Bonnington, O., Harrison, G., Hickman, M., & Irving, W. L. (in press). Understanding hepatitis C intervention success: qualitative findings from the HepCATT study. Journal of Viral Hepatitis, doi:10.1111/jvh.12869

The United Kingdom has committed to eliminating viral hepatitis as a public health threat. Innovative interventions for marginalized populations are required to realize this goal. In 2016, the HepCATT study team implemented a complex hepatitis C (HCV... Read More about Understanding hepatitis C intervention success: qualitative findings from the HepCATT study.

Physiological, but not fitness, effects of two interacting haemoparasitic infections in a wild rodent (2018)
Journal Article
Taylor, C. H., Wanelik, K. M., Friberg, I. M., Lowe, A., Hall, A. J., Ralli, C., …Bradley, J. E. (2018). Physiological, but not fitness, effects of two interacting haemoparasitic infections in a wild rodent. International Journal for Parasitology, 48(6), 463-471. https://doi.org/10.1016/j.ijpara.2017.11.006

In contrast to the conditions in most laboratory studies, wild animals are routinely challenged by multiple infections at once, and these infections can interact in complex ways. This means that the impact of a parasite on its host’s physiology and f... Read More about Physiological, but not fitness, effects of two interacting haemoparasitic infections in a wild rodent.

Micro-pharmacokinetics: quantifying local drug concentration at live cell membranes (2018)
Journal Article
Gherbi, K., Briddon, S. J., & Charlton, S. J. (in press). Micro-pharmacokinetics: quantifying local drug concentration at live cell membranes. Scientific Reports, 8, doi:10.1038/s41598-018-21100-x

Fundamental equations for determining pharmacological parameters, such as the binding afnity of a ligand for its target receptor, assume a homogeneous distribution of ligand, with concentrations in the immediate vicinity of the receptor being the sam... Read More about Micro-pharmacokinetics: quantifying local drug concentration at live cell membranes.

Prophylactic antibiotic use in COPD and the potential anti-inflammatory activities of antibiotics (2018)
Journal Article
Huckle, A. W., Fairclough, L. C., & Todd, I. (2018). Prophylactic antibiotic use in COPD and the potential anti-inflammatory activities of antibiotics. Current Respiratory Care Reports, 63(5), doi:10.4187/respcare.05943

Antibiotics have previously demonstrated anti-inflammatory properties, and they have been linked to therapeutic benefit in several pulmonary conditions that feature inflammation. Previous research suggests that these anti-inflammatory properties may... Read More about Prophylactic antibiotic use in COPD and the potential anti-inflammatory activities of antibiotics.