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The adherence-associated Fdp fasciclin I domain protein of the biohydrogen producer Rhodobacter sphaeroides is regulated by the global Prr pathway (2020)
Journal Article
Jeong, E., Broad, S., Moody, R., & Phillips-Jones, M. (2020). The adherence-associated Fdp fasciclin I domain protein of the biohydrogen producer Rhodobacter sphaeroides is regulated by the global Prr pathway. International Journal of Hydrogen Energy, 45(51), 26840-26854. https://doi.org/10.1016/j.ijhydene.2020.07.108

Expression of fdp, encoding a fasciclin I domain protein important for adherence in the hydrogenproducing bacterium Rhodobacter sphaeroides, was investigated under a range of conditions to gain insights into optimization of adherence for immobilizati... Read More about The adherence-associated Fdp fasciclin I domain protein of the biohydrogen producer Rhodobacter sphaeroides is regulated by the global Prr pathway.

Glycoconjugate vaccines: some observations on carrier and production methods (2020)
Journal Article
MacCalman, T. E., Phillips-Jones, M. K., & Harding, S. E. (2020). Glycoconjugate vaccines: some observations on carrier and production methods. Biotechnology and Genetic Engineering Reviews, 35(2), 93-125. https://doi.org/10.1080/02648725.2019.1703614

© 2019 Informa UK Limited, trading as Taylor & Francis Group. Glycoconjugate vaccines use protein carriers to improve the immune response to polysaccharide antigens. The protein component allows the vaccine to interact with T cells, providing a str... Read More about Glycoconjugate vaccines: some observations on carrier and production methods.

The antibiotic vancomycin induces complexation and aggregation of gastrointestinal and submaxillary mucins (2020)
Journal Article
Dinu, V., Lu, Y., Weston, N., Lithgo, R., Coupe, H., Channell, G., …Harding, S. E. (2020). The antibiotic vancomycin induces complexation and aggregation of gastrointestinal and submaxillary mucins. Scientific Reports, 10(1), Article 960. https://doi.org/10.1038/s41598-020-57776-3

© 2020, The Author(s). Vancomycin, a branched tricyclic glycosylated peptide antibiotic, is a last-line defence against serious infections caused by staphylococci, enterococci and other Gram-positive bacteria. Orally-administered vancomycin is the dr... Read More about The antibiotic vancomycin induces complexation and aggregation of gastrointestinal and submaxillary mucins.

The gelatinase biosynthesis‐activating pheromone binds and stabilises the FsrB membrane protein in Enterococcus faecalis quorum sensing (2019)
Journal Article
Littlewood, S., Tattersall, H., Hughes, C. S., Hussain, R., Ma, P., Harding, S. E., …Phillips‐Jones, M. K. (2020). The gelatinase biosynthesis‐activating pheromone binds and stabilises the FsrB membrane protein in Enterococcus faecalis quorum sensing. FEBS Letters, 594(3), 553-563. https://doi.org/10.1002/1873-3468.13634

Quorum‐sensing mechanisms regulate gene expression in response to changing cell‐population density detected through pheromones. In Enterococcus faecalis, Fsr quorum sensing produces and responds to the gelatinase biosynthesis‐activating pheromone (GB... Read More about The gelatinase biosynthesis‐activating pheromone binds and stabilises the FsrB membrane protein in Enterococcus faecalis quorum sensing.

Tapping into synchrotron and benchtop circular dichroism spectroscopy for expanding studies of complex polysaccharides and their interactions in anoxic archaeological wood (2019)
Journal Article
Phillips-Jones, M. K., & Harding, S. E. (2019). Tapping into synchrotron and benchtop circular dichroism spectroscopy for expanding studies of complex polysaccharides and their interactions in anoxic archaeological wood. Heritage, 2(1), 121-134. https://doi.org/10.3390/heritage2010009

Circular dichroism (CD) (and synchrotron circular dichroism (SCD)) spectroscopy is a rapid, highly sensitive technique used to investigate structural conformational changes in biomolecules in response to interactions with ligands in solution and in f... Read More about Tapping into synchrotron and benchtop circular dichroism spectroscopy for expanding studies of complex polysaccharides and their interactions in anoxic archaeological wood.

The discovery of hydrogen bonds in DNA and a re-evaluation of the 1948 Creeth two-chain model for its structure (2018)
Journal Article
Harding, S. E., Channell, G., & Phillips-Jones, M. K. (2018). The discovery of hydrogen bonds in DNA and a re-evaluation of the 1948 Creeth two-chain model for its structure. Biochemical Society Transactions, 46(5), 1171-1182. https://doi.org/10.1042/BST20180158

We recall the experimental approaches involved in the discovery of hydrogen bonds in deoxyribonucleic acid (DNA) made 70 years ago by a team of scientists at University College Nottingham led by J.M. Gulland, and in relation to previous studies. This... Read More about The discovery of hydrogen bonds in DNA and a re-evaluation of the 1948 Creeth two-chain model for its structure.

Production of membrane proteins for characterisation of their pheromone-sensing and antimicrobial resistance functions (2018)
Journal Article
Azam, A. A., Kinder, J. M., Khan, G. N., Alase, A., Ma, P., Liu, Y., …Phillips-Jones, M. K. (2018). Production of membrane proteins for characterisation of their pheromone-sensing and antimicrobial resistance functions. European Biophysics Journal, https://doi.org/10.1007/s00249-018-1325-z

Despite the importance of membrane proteins in cellular processes, studies of these hydrophobic proteins present major technical challenges, including expression and purification for structural and biophysical studies. A modified strategy of that pro... Read More about Production of membrane proteins for characterisation of their pheromone-sensing and antimicrobial resistance functions.

Antimicrobial resistance (AMR) nanomachines—mechanisms for fluoroquinolone and glycopeptide recognition, efflux and/or deactivation (2018)
Journal Article
Phillips-Jones, M. K., & Harding, S. E. (2018). Antimicrobial resistance (AMR) nanomachines—mechanisms for fluoroquinolone and glycopeptide recognition, efflux and/or deactivation. Biophysical Reviews, 10(2), 347-362. https://doi.org/10.1007/s12551-018-0404-9

In this review, we discuss mechanisms of resistance identified in bacterial agents Staphylococcus aureus and the enterococci towards two priority classes of antibiotics—the fluoroquinolones and the glycopeptides. Members of both classes interact with... Read More about Antimicrobial resistance (AMR) nanomachines—mechanisms for fluoroquinolone and glycopeptide recognition, efflux and/or deactivation.

Full hydrodynamic reversibility of the weak dimerization of vancomycin and elucidation of its interaction with VanS monomers at clinical concentration (2017)
Journal Article
Phillips-Jones, M. K., Lithgo, R., Dinu, V., Gillis, R. B., Harding, J. E., Adams, G. G., & Harding, S. E. (2017). Full hydrodynamic reversibility of the weak dimerization of vancomycin and elucidation of its interaction with VanS monomers at clinical concentration. Scientific Reports, 7(1), 1-10. https://doi.org/10.1038/s41598-017-12620-z

The reversibility and strength of the previously established dimerization of the important glycopeptide antibiotic vancomycin in four different aqueous solvents (including a medically-used formulation) have been studied using short-column sedimentati... Read More about Full hydrodynamic reversibility of the weak dimerization of vancomycin and elucidation of its interaction with VanS monomers at clinical concentration.

Hydrodynamics of the VanA-type VanS histidine kinase: an extended solution conformation and first evidence for interactions with vancomycin (2017)
Journal Article
Phillips-Jones, M. K., Channell, G. A., Kelsall, C. J., Hughes, C. S., Ashcroft, A. E., Patching, S. G., …Harding, S. E. (2017). Hydrodynamics of the VanA-type VanS histidine kinase: an extended solution conformation and first evidence for interactions with vancomycin. Scientific Reports, 7(1), 1-12. https://doi.org/10.1038/srep46180

VanA-type resistance to glycopeptide antibiotics in clinical enterococci is regulated by the VanSARA two-component signal transduction system. The nature of the molecular ligand that is recognised by the VanSA sensory component has not hitherto been... Read More about Hydrodynamics of the VanA-type VanS histidine kinase: an extended solution conformation and first evidence for interactions with vancomycin.

Purification of bacterial membrane sensor kinases and biophysical methods for determination of their ligand and inhibitor interactions (2016)
Journal Article
Hussain, R., Harding, S. E., Hughes, C. S., Ma, P., Patching, S. G., Edara, S., …Phillips-Jones, M. K. (2016). Purification of bacterial membrane sensor kinases and biophysical methods for determination of their ligand and inhibitor interactions. Biochemical Society Transactions, 44(3), 810-823. https://doi.org/10.1042/BST20160023

This article reviews current methods for the reliable heterologous overexpression in Escherichia coli and purification of milligram quantities of bacterial membrane sensor kinase (MSK) proteins belonging to the two-component signal transduction famil... Read More about Purification of bacterial membrane sensor kinases and biophysical methods for determination of their ligand and inhibitor interactions.