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The gelatinase biosynthesis‐activating pheromone binds and stabilises the FsrB membrane protein in Enterococcus faecalis quorum sensing (2019)
Journal Article
Littlewood, S., Tattersall, H., Hughes, C. S., Hussain, R., Ma, P., Harding, S. E., …Phillips‐Jones, M. K. (2019). The gelatinase biosynthesis‐activating pheromone binds and stabilises the FsrB membrane protein in Enterococcus faecalis quorum sensing. FEBS Letters, https://doi.org/10.1002/1873-3468.13634

Quorum‐sensing mechanisms regulate gene expression in response to changing cell‐population density detected through pheromones. In Enterococcus faecalis, Fsr quorum sensing produces and responds to the gelatinase biosynthesis‐activating pheromone (GB... Read More about The gelatinase biosynthesis‐activating pheromone binds and stabilises the FsrB membrane protein in Enterococcus faecalis quorum sensing.

Characterisation of the molecular properties of scleroglucan as an alternative rigid rod molecule to xanthan gum for oropharyngeal dysphagia (2019)
Journal Article
Li, X., Lu, Y., Adams, G. G., Zobel, H., Ballance, S., Wolf, B., & Harding, S. E. (2020). Characterisation of the molecular properties of scleroglucan as an alternative rigid rod molecule to xanthan gum for oropharyngeal dysphagia. Food Hydrocolloids, 101, 1-9. https://doi.org/10.1016/j.foodhyd.2019.105446

Scleroglucan, a neutral β(1–3) glucan with β(1–6) glucan branches every third residue, is being considered as an alternative rod-like, shear thinning high molecular weight β-glucan based polysaccharide to xanthan gum for the management of patients wi... Read More about Characterisation of the molecular properties of scleroglucan as an alternative rigid rod molecule to xanthan gum for oropharyngeal dysphagia.

Resistance mechanisms adopted by a Salmonella Typhimurium mutant against bacteriophage (2019)
Journal Article
Wang, C., Nie, T., Lin, F., Connerton, I. F., Lu, Z., Zhou, S., & Hang, H. (2019). Resistance mechanisms adopted by a Salmonella Typhimurium mutant against bacteriophage. Virus Research, 273, https://doi.org/10.1016/j.virusres.2019.197759

© 2019 Elsevier B.V. Bacteriophages have key roles in regulating bacterial populations in most habitats. A Salmonella Typhimurium mutant (N18) with impaired sensitivity to phage fmb-p1 was obtained and examined, the adsorption efficiency of fmb-p1 to... Read More about Resistance mechanisms adopted by a Salmonella Typhimurium mutant against bacteriophage.

Characterisation of high molecular weight hop proanthocyanidins using Analytical Ultracentrifugation (2019)
Journal Article
Gadon, A., Linforth, R., Harding, S. E., & Cook, D. (2019). Characterisation of high molecular weight hop proanthocyanidins using Analytical Ultracentrifugation. Scientific Reports, 9(1), https://doi.org/10.1038/s41598-019-49171-4

We report the novel application of Analytical Ultracentrifugation (AUCF) to characterise the polymeric proanthocyanidin fraction of hops. Extraction of hop samples with 70% acetone (aq) followed by a C-18 Solid Phase Extraction yielded polyphenolic f... Read More about Characterisation of high molecular weight hop proanthocyanidins using Analytical Ultracentrifugation.

Development of a comprehensive protein microarray for immunoglobulin E profiling in horses with severe asthma (2019)
Journal Article
Moore‐Colyer, M., White, S., Moore-Colyer, M., Marti, E., Coüetil, L., Hannant, D., …Alcocer, M. (2019). Development of a comprehensive protein microarray for immunoglobulin E profiling in horses with severe asthma. Journal of Veterinary Internal Medicine, doi:10.1111/jvim.15564

Background Severe asthma in horses, known as severe equine asthma (SEA), is a prevalent, performance‐limiting disease associated with increased allergen‐specific immunoglobulin E (IgE) against a range of environmental aeroallergens. Objective To... Read More about Development of a comprehensive protein microarray for immunoglobulin E profiling in horses with severe asthma.

Use of molecular crowding for the detection of protein self-association by size-exclusion chromatography (2019)
Journal Article
Mrozowich, T., Winzor, D. J., Scott, D. J., & Patel, T. R. (2019). Use of molecular crowding for the detection of protein self-association by size-exclusion chromatography. Analytical Biochemistry, 584, https://doi.org/10.1016/j.ab.2019.113392

The feasibility of employing molecular crowding cosolutes to facilitate the detection of protein self-association by zonal size exclusion chromatography is investigated. Theoretical considerations have established that although the cosolute-induced d... Read More about Use of molecular crowding for the detection of protein self-association by size-exclusion chromatography.

Spray-dried anti-Campylobacter bacteriophage CP30A powder suitable for global distribution without cold chain infrastructure (2019)
Journal Article
Carrigy, N. B., Liang, L., Wang, H., Kariukic, S., Nagel, T. E. .., Connerton, I. F., & Vehringa, R. (2019). Spray-dried anti-Campylobacter bacteriophage CP30A powder suitable for global distribution without cold chain infrastructure. International Journal of Pharmaceutics, 569, https://doi.org/10.1016/j.ijpharm.2019.118601

Campylobacter jejuni is a leading cause of foodborne illness globally. In this study, a spray drying and packaging process was developed to produce a thermally-stable dry powder containing bacteriophages that retains biological activity against C. je... Read More about Spray-dried anti-Campylobacter bacteriophage CP30A powder suitable for global distribution without cold chain infrastructure.

Towards a surrogate system to express human lipid binding TCRs (2019)
Journal Article
Wang, R., Pscheid, R., Ghumra, A., Kan, L. Y. L., Cochrane, S., Fairclough, L., & Alcocer, M. J. C. (2019). Towards a surrogate system to express human lipid binding TCRs. Biotechnology Letters, doi:10.1007/s10529-019-02713-2

Background Previously we reported that natural nut lipids were necessary for sensitization and that natural killer T cells (NKTs) must play a critical role in the development of food allergic responses. A major bottleneck in further understanding... Read More about Towards a surrogate system to express human lipid binding TCRs.

Mucin immobilization in calcium alginate: A possible mucus mimetic tool for evaluating mucoadhesion and retention of flavour (2019)
Journal Article
Dinu, V., Yakubov, G. E., Lim, M., Hurst, K., Adams, G. G., Harding, S. E., & Fisk, I. D. (2019). Mucin immobilization in calcium alginate: A possible mucus mimetic tool for evaluating mucoadhesion and retention of flavour. International Journal of Biological Macromolecules, 138, 831-836. https://doi.org/10.1016/j.ijbiomac.2019.07.148

To reduce animal testing, there is a need to develop novel in-vitro models for evaluating the retention of bioactive compounds in food and pharmaceutical products. Here, a mucus-mimetic platform was developed through a one-step approach based on enca... Read More about Mucin immobilization in calcium alginate: A possible mucus mimetic tool for evaluating mucoadhesion and retention of flavour.

Understanding the influence of processing conditions on the extraction of rhamnogalacturonan-I “hairy” pectin from sugar beet pulp (2019)
Journal Article
Mao, Y., Lei, R., Ryan, J., Arrutia Rodriguez, F., Rastall, B., Chatzifragkou, A., …Binner, E. (2019). Understanding the influence of processing conditions on the extraction of rhamnogalacturonan-I “hairy” pectin from sugar beet pulp. Food Chemistry: X, 2, doi:10.1016/j.fochx.2019.100026

Sugar beet pectin is rich in rhamnogalacturonan-I (RG-I) region, which is a potential source of prebiotics. RG-I pectin cannot be extracted the same way as commercial homogalacturan-rich pectin using hot acid. Therefore, this study has explored sever... Read More about Understanding the influence of processing conditions on the extraction of rhamnogalacturonan-I “hairy” pectin from sugar beet pulp.

Submaxillary Mucin: its Effect on Aroma Release from Acidic Drinks and New Insight into the Effect of Aroma Compounds on its Macromolecular Integrity (2019)
Journal Article
Dinu, V., Gillis, R. B., MacCalman, T., Lim, M., Adams, G. G., Harding, S. E., & Fisk, I. D. (2019). Submaxillary Mucin: its Effect on Aroma Release from Acidic Drinks and New Insight into the Effect of Aroma Compounds on its Macromolecular Integrity. Food Biophysics, 14(3), doi:10.1007/s11483-019-09574-2

Submaxillary mucin is a major component that defines the makeup and functionality of saliva. Understanding its structure and function during food intake is key to designing appropriate strategies for enhancing the delivery of flavour. In the present... Read More about Submaxillary Mucin: its Effect on Aroma Release from Acidic Drinks and New Insight into the Effect of Aroma Compounds on its Macromolecular Integrity.

Understanding “corruption” in regulatory agencies: The case of food inspection in Saudi Arabia (2019)
Journal Article
Al-Mutairi, S., Connerton, I., & Dingwall, R. (2019). Understanding “corruption” in regulatory agencies: The case of food inspection in Saudi Arabia. Regulation and Governance, 13(4), 507-519. https://doi.org/10.1111/rego.12247

Corruption is a relatively neglected topic in studies of regulatory agencies. The label is applied to a wide range of deviations from behavioral standards ultimately derived from Weber's account of the ideals of Prussian bureaucracy. This paper draws... Read More about Understanding “corruption” in regulatory agencies: The case of food inspection in Saudi Arabia.

Varying magnesium concentration elicits changes in inflammatory response in human umbilical vein endothelial cells (HUVECs) (2018)
Journal Article
Almousa, L. A., Salter, A. M., & Langley-Evans, S. C. (2018). Varying magnesium concentration elicits changes in inflammatory response in human umbilical vein endothelial cells (HUVECs). Magnesium Research, 31(3), 99-109. doi:10.1684/mrh.2018.0439

The aims of this study were to determine whether low concentrations of magnesium in vitro exacerbated the human umbilical vein endothelial cell (HUVEC) response to inflammatory challenge, and whether expression of the nuclear factor kappa-light-chain... Read More about Varying magnesium concentration elicits changes in inflammatory response in human umbilical vein endothelial cells (HUVECs).

The discovery of hydrogen bonds in DNA and a re-evaluation of the 1948 Creeth two-chain model for its structure (2018)
Journal Article
Harding, S. E., Channell, G., & Phillips-Jones, M. K. (2018). The discovery of hydrogen bonds in DNA and a re-evaluation of the 1948 Creeth two-chain model for its structure. Biochemical Society Transactions, 46(5), 1171-1182. https://doi.org/10.1042/BST20180158

We recall the experimental approaches involved in the discovery of hydrogen bonds in deoxyribonucleic acid (DNA) made 70 years ago by a team of scientists at University College Nottingham led by J.M. Gulland, and in relation to previous studies. This... Read More about The discovery of hydrogen bonds in DNA and a re-evaluation of the 1948 Creeth two-chain model for its structure.

Public health strategies to reduce sugar intake in the UK: An exploration of public perceptions using digital spaces (2018)
Journal Article
Swift, J. A., Strathearn, L., Morris, A., Chi, Y., Townsend, T., & Pearce, J. (2018). Public health strategies to reduce sugar intake in the UK: An exploration of public perceptions using digital spaces. Nutrition Bulletin, 43(3), 238-247. https://doi.org/10.1111/nbu.12346

Background: To explore UK public perceptions of children’s sugar consumption, Public Health England’s Change4Life Sugar Smart App and the Soft Drinks Industry Levy, using solicited and unsolicited digital data. Methods: Data from three digital space... Read More about Public health strategies to reduce sugar intake in the UK: An exploration of public perceptions using digital spaces.

A proposed biomass char classification system (2018)
Journal Article
Lester, E., Avila, C., Pang, C. H., Williams, O., Perkins, J., Gaddipatti⁠, S., …Wu, T. (2018). A proposed biomass char classification system. Fuel, 232, doi:10.1016/j.fuel.2018.05.153

A new classification system is proposed for the morphological characterisation of char structures from biomass. These char structures are unlike the coal chars that have an established nomenclature via the International Committee of Coal and Organic... Read More about A proposed biomass char classification system.

Garlic and gaseous mediators (2018)
Journal Article
Rose, P., Moore, P. K., & Zhu, Y. (in press). Garlic and gaseous mediators. Trends in Pharmacological Sciences, doi:10.1016/j.tips.2018.03.009

Garlic (Allium sativum) and allied plant species are rich sources of sulfur compounds. Major roles for garlic and its sulfur constituents include the regulation of vascular homeostasis and the control of metabolic systems linked to nutrient metabolis... Read More about Garlic and gaseous mediators.

Maternal obesity-induced decreases in plasma, hepatic and uterine polyunsaturated fatty acids during labour is reversed through improved nutrition at conception (2018)
Journal Article
Muir, R. D., Liu, G., Khan, R., Shmygol, A., Quenby, S., Gibson, R. A., …Elmes, M. (2018). Maternal obesity-induced decreases in plasma, hepatic and uterine polyunsaturated fatty acids during labour is reversed through improved nutrition at conception. Scientific Reports, 8, doi:10.1038/s41598-018-21809-9

Maternal obesity is associated with prolonged and dysfunctional labour, potentially through decreased synthesis of prostaglandins that stimulate myometrial contractions. We assessed the impact of maternal obesity on concentrations of precursor fatty... Read More about Maternal obesity-induced decreases in plasma, hepatic and uterine polyunsaturated fatty acids during labour is reversed through improved nutrition at conception.