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Genetic diversity of Koala retrovirus (KoRV) env gene subtypes: Insights into northern and southern koala populations (2019)
Journal Article
Sarker, N., Fabijan, J., Seddon, J., Tarlinton, R., Owen, H., Simmons, G., …Meers, J. (2019). Genetic diversity of Koala retrovirus (KoRV) env gene subtypes: Insights into northern and southern koala populations. Journal of General Virology, 100(9), https://doi.org/10.1099/jgv.0.001304

Koala retrovirus (KoRV) is a recently endogenised retrovirus associated with neoplasia and immunosuppression in koala populations. The virus is known to display sequence variability and to be present at varying prevalence in different populations, wi... Read More about Genetic diversity of Koala retrovirus (KoRV) env gene subtypes: Insights into northern and southern koala populations.

The cross-talk between miR-511-3p and c-type lectin receptors on dendritic cells affects dendritic cell function (2019)
Journal Article
Awuah, D., Alobaid, M., Latif, A., Salazar, F., Emes, R. D., & Ghaemmaghami, A. M. (2019). The cross-talk between miR-511-3p and c-type lectin receptors on dendritic cells affects dendritic cell function. Journal of Immunology, 203(1), 148-157. doi:10.4049/jimmunol.1801108

MicroRNAs are small, noncoding RNAs that function as posttranscriptional modulators of gene expression by binding target mRNAs and inhibiting translation. They are therefore crucial regulators of several biological as well as immunological events. Re... Read More about The cross-talk between miR-511-3p and c-type lectin receptors on dendritic cells affects dendritic cell function.

Detection and analysis of RNA methylation (2019)
Journal Article
Mongan, N. P., Emes, R. D., & Archer, N. (2019). Detection and analysis of RNA methylation. F1000Research, 8, 559. doi:10.12688/f1000research.17956.1

Evolution of gene expression levels in the male reproductive organs of Anopheles mosquitoes (2019)
Journal Article
Izquierdo, A., Fahrenberger, M., Persampieri, T., Benedict, M. Q., Giles, T., Catteruccia, F., …Dottorini, T. (2019). Evolution of gene expression levels in the male reproductive organs of Anopheles mosquitoes. Life Science Alliance, 2(1), doi:10.26508/lsa.201800191

Modifications in gene expression determine many of the phenotypic differentiations between closely related species. This is particularly evident in reproductive tissues, where evolution of genes is more rapid, facilitating the appearance of distinct... Read More about Evolution of gene expression levels in the male reproductive organs of Anopheles mosquitoes.

Discrimination of contagious and environmental strains of Streptococcus uberis in dairy herds by means of mass spectrometry and machine-learning (2018)
Journal Article
Esener, N., Green, M. J., Emes, R. D., Jowett, B., Davies, P. L., Bradley, A. J., & Dottorini, T. (2018). Discrimination of contagious and environmental strains of Streptococcus uberis in dairy herds by means of mass spectrometry and machine-learning. Scientific Reports, 8(1), doi:10.1038/s41598-018-35867-6

Streptococcus uberis is one of the most common pathogens of clinical mastitis in the dairy industry. Knowledge of pathogen transmission route is essential for the selection of the most suitable intervention. Here we show that spectral profiles acquir... Read More about Discrimination of contagious and environmental strains of Streptococcus uberis in dairy herds by means of mass spectrometry and machine-learning.

The beta-adrenergic agonist, Ractopamine increases skeletal muscle expression of Asparagine Synthetase as part of an integrated stress response gene program (2018)
Journal Article
Brown, D., Ryan, K., Daniel, Z., Mareko, M., Talbot, R., Moreton, J., …Brameld, J. M. (2018). The beta-adrenergic agonist, Ractopamine increases skeletal muscle expression of Asparagine Synthetase as part of an integrated stress response gene program. Scientific Reports, 8, doi:10.1038/s41598-018-34315-9

Synthetic beta-adrenergic agonists (BA) have broad biomedical and agricultural application for increasing lean body mass, yet a poor understanding of the biology underpinning these agents is limiting further drug discovery potential. Growing female p... Read More about The beta-adrenergic agonist, Ractopamine increases skeletal muscle expression of Asparagine Synthetase as part of an integrated stress response gene program.

Acute Toxoplasma gondii infection in cats induced tissue-specific transcriptional response dominated by immune signatures (2018)
Journal Article
Cong, W., Dottorini, T., Khan, F., Emes, R. D., Zhang, F., Zhou, C., …Zhu, X. (2018). Acute Toxoplasma gondii infection in cats induced tissue-specific transcriptional response dominated by immune signatures. Frontiers in Immunology, 9, doi:10.3389/fimmu.2018.02403. ISSN 1664-3224

RNA-sequencing was used to detect transcriptional changes in six tissues of cats, seven days after T. gondii infection. A total of 737 genes were differentially expressed (DEGs), of which 410 were up-regulated and 327 were down-regulated. The liver e... Read More about Acute Toxoplasma gondii infection in cats induced tissue-specific transcriptional response dominated by immune signatures.

Paternal diet programs offspring health through sperm- and seminal plasma-specific pathways in mice (2018)
Journal Article
Watkins, A. J., Dias, I., Tsuro, H., Allen, D., Emes, R. D., Moreton, J., …Sinclair, K. D. (2018). Paternal diet programs offspring health through sperm- and seminal plasma-specific pathways in mice. Proceedings of the National Academy of Sciences, 115(40), (10064–10069). doi:10.1073/pnas.1806333115. ISSN 1091-6490

The association between poor paternal diet, perturbed embryonic development, and adult offspring ill health represents a new focus for the Developmental Origins of Health and Disease hypothesis. However, our understanding of the underlying mechanisms... Read More about Paternal diet programs offspring health through sperm- and seminal plasma-specific pathways in mice.

The applied development of a tiered multilocus sequence typing (MLST) scheme for Dichelobacter nodosus (2018)
Journal Article
Blanchard, A. M., Jolley, K. A., Maiden, M. C., Coffey, T. J., Maboni, G., Staley, C. E., …Tötemeyer, S. (in press). The applied development of a tiered multilocus sequence typing (MLST) scheme for Dichelobacter nodosus. Frontiers in Microbiology, 9, doi:10.3389/fmicb.2018.00551

Dichelobacter nodosus (D. nodosus) is the causative pathogen of ovine footrot, a disease that has a significant welfare and financial impact on the global sheep industry. Previous studies into the phylogenetics of D. nodosus have focused on Australia... Read More about The applied development of a tiered multilocus sequence typing (MLST) scheme for Dichelobacter nodosus.

Cancer reversion with oocyte extracts is mediated by cell cycle arrest and induction of tumour dormancy (2018)
Journal Article
Saad, N., Alberio, R., Johnson, A. D., Emes, R. D., Giles, T. C., Clarke, P., …Allegrucci, C. (2018). Cancer reversion with oocyte extracts is mediated by cell cycle arrest and induction of tumour dormancy. Oncotarget, 9(22), doi:10.18632/oncotarget.24664

Inducing stable control of tumour growth by tumour reversion is an alternative approach to cancer treatment when eradication of the disease cannot be achieved. The process requires re-establishment of normal control mechanisms that are lost in canc... Read More about Cancer reversion with oocyte extracts is mediated by cell cycle arrest and induction of tumour dormancy.

Opsonic phagocytosis in chronic obstructive pulmonary disease is enhanced by Nrf2 agonists (2018)
Journal Article
Bewley, M. A., Budd, R. C., Ryan, E., Cole, J., Collini, P., Marshall, J., …Dockrell, D. H. (in press). Opsonic phagocytosis in chronic obstructive pulmonary disease is enhanced by Nrf2 agonists. American Journal of Respiratory and Critical Care Medicine, doi:10.1164/rccm.201705-0903OC

Rationale: Previous studies have identified defects in bacterial phagocytosis by alveolar macrophages (AM) in patients with chronic obstructive pulmonary disease (COPD) but the mechanisms and clinical consequences remain incompletely defined. Object... Read More about Opsonic phagocytosis in chronic obstructive pulmonary disease is enhanced by Nrf2 agonists.

Identification of stable reference genes for quantitative PCR in koalas (2018)
Journal Article
Sarker, N., Fabijan, J., Emes, R. D., Hemmatzadeh, F., Meers, J., Moreton, J., …Tarlinton, R. E. (in press). Identification of stable reference genes for quantitative PCR in koalas. Scientific Reports, 8, doi:10.1038/s41598-018-21723-0

To better understand host and immune response to diseases, gene expression studies require identification of reference genes with stable expression for accurate normalisation. This study describes the identification and testing of reference genes wit... Read More about Identification of stable reference genes for quantitative PCR in koalas.

Maternal genome-wide DNA methylation profiling in gestational diabetes shows distinctive disease-associated changes relative to matched healthy pregnancies (2018)
Journal Article
Hanna, F. W., Wu, P., Farrell, W. E., Haworth, K. E., Emes, R. D., Kitchen, M. O., …Fryer, A. A. (2018). Maternal genome-wide DNA methylation profiling in gestational diabetes shows distinctive disease-associated changes relative to matched healthy pregnancies. Epigenetics, 13(2), 122-128. https://doi.org/10.1080/15592294.2016.1166321

Several recent reports have described associations between gestational diabetes (GDM) and changes to the epigenomic landscape where the DNA samples were derived from either cord or placental sources. We employed genome-wide 450Karray analysis to dete... Read More about Maternal genome-wide DNA methylation profiling in gestational diabetes shows distinctive disease-associated changes relative to matched healthy pregnancies.

HumanMethylation450K array–identified biomarkers predict tumour recurrence/progression at initial diagnosis of high-risk non-muscle Invasive bladder cancer (2018)
Journal Article
Kitchen, M. O., Bryan, R. T., Emes, R. D., Luscombe, C. J., Cheng, K., Zeegers, M. P., …Fryer, A. A. (in press). HumanMethylation450K array–identified biomarkers predict tumour recurrence/progression at initial diagnosis of high-risk non-muscle Invasive bladder cancer. Biomarkers in Cancer,

Background: High-risk non-muscle invasive bladder cancer (HR-NMIBC) is a clinically unpredictable disease. Despite clinical risk estimation tools, many patients are undertreated with intra-vesical therapies alone, whereas others may be over-treated w... Read More about HumanMethylation450K array–identified biomarkers predict tumour recurrence/progression at initial diagnosis of high-risk non-muscle Invasive bladder cancer.

Complete genome sequences of seven Vibrio cholerae phages isolated in China (2017)
Journal Article
Bhandare, S. G., Warry, A., Emes, R. D., Su, J., Barrow, P. A., & Atterbury, R. J. (2017). Complete genome sequences of seven Vibrio cholerae phages isolated in China. Genome Announcements, 5(47), doi:10.1128/genomeA.01019-17

The complete genome sequences of seven closely related Vibrio cholerae phages isolated from environmental sites in southeastern China are reported here. Phages QH, CJY, H1, H2, H3, J2, and J3 are members of the Podoviridae family and are highly simil... Read More about Complete genome sequences of seven Vibrio cholerae phages isolated in China.

Complete genome sequences of Vibrio cholera: specific bacteriophages 24 and X29 (2017)
Journal Article
Bhandare, S. G., Warry, A., Emes, R. D., Hooton, S. P., Barrow, P. A., & Atterbury, R. J. (2017). Complete genome sequences of Vibrio cholera: specific bacteriophages 24 and X29. Genome Announcements, 5(46), doi:10.1128/genomeA.01013-17

The complete genomes of two Vibrio cholerae bacteriophages of potential interest for cholera bacteriophage (phage) therapy were sequenced and annotated. The genome size of phage 24 is 44,395 bp encoding 71 putative proteins, and that of phage X29 is... Read More about Complete genome sequences of Vibrio cholera: specific bacteriophages 24 and X29.

A proteomic investigation into mechanisms underpinning corticosteroid effects on neural stem cells (2017)
Journal Article
Al-Mayyahi, R. S., Sterio, L. D., Connolly, J. B., Adams, C. F., Al-Tumah, W. A., Sen, J., …Chari, D. M. (2018). A proteomic investigation into mechanisms underpinning corticosteroid effects on neural stem cells. Molecular and Cellular Neuroscience, 86, doi:10.1016/j.mcn.2017.11.006

Corticosteroids (CSs) are widely used clinically, for example in pediatric respiratory distress syndrome, and immunosuppression to prevent rejection of stem cell transplant populations in neural cell therapy. However, such treatment can be associated... Read More about A proteomic investigation into mechanisms underpinning corticosteroid effects on neural stem cells.

Unbiased analysis of the impact of micropatterned biomaterials on macrophage behaviour provides insights beyond pre-defined polarisation states (2017)
Journal Article
Singh, S., Awuah, D., Rostam, H., Emes, R., Kandola, N. K., Onion, D., …Ghaemmaghami, A. M. (in press). Unbiased analysis of the impact of micropatterned biomaterials on macrophage behaviour provides insights beyond pre-defined polarisation states. ACS Biomaterials Science and Engineering, doi:10.1021/acsbiomaterials.7b00104

Macrophages are master regulators of immune responses towards implanted biomaterials. The activation state adopted by macrophages in response to biomaterials determines their own phenotype and function as well as those of other resident and infiltrat... Read More about Unbiased analysis of the impact of micropatterned biomaterials on macrophage behaviour provides insights beyond pre-defined polarisation states.

A distinct bacterial dysbiosis associated skin inflammation in ovine footrot (2017)
Journal Article
Maboni, G., Blanchard, A. M., Frosth, S., Stewart, C. E., Emes, R. D., & Tötemeyer, S. (in press). A distinct bacterial dysbiosis associated skin inflammation in ovine footrot. Scientific Reports, 7, doi:10.1038/srep45220

Ovine footrot is a highly prevalent bacterial disease caused by Dichelobacter nodosus and characterised by the separation of the hoof horn from the underlying skin. The role of innate immune molecules and other bacterial communities in the developmen... Read More about A distinct bacterial dysbiosis associated skin inflammation in ovine footrot.

Evolution of complexity in the zebrafish synapse proteome (2017)
Journal Article
Grant, S. G. N., Reig-Viader, R., Bayés, À., Bayes, A., Collins, M. O., Reig-Vader, R., …Grant, S. G. (in press). Evolution of complexity in the zebrafish synapse proteome. Nature Communications, 8, https://doi.org/10.1038/ncomms14613

The proteome of human brain synapses is highly complex and mutated in over 130 diseases. This complexity arose from two whole genome duplications early in the vertebrate lineage. Zebrafish are used in modelling human diseases, however its synapse pro... Read More about Evolution of complexity in the zebrafish synapse proteome.