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Localized induction of gene expression in embryonic stem cell aggregates using holographic optical tweezers to create biochemical gradients (2019)
Journal Article
Kirkham, G. R., Ware, J., Upton, T., Allen, S., Shakesheff, K. M., & Buttery, L. D. (2019). Localized induction of gene expression in embryonic stem cell aggregates using holographic optical tweezers to create biochemical gradients. Regenerative Engineering and Translational Medicine, doi:10.1007/s40883-019-00114-5

Three dimensional (3D) cell models that mimic the structure and function of native tissues are enabling more detailed study of physiological and pathological mechanisms in vitro. We have previously demonstrated the ability to build and manipulate 3D... Read More about Localized induction of gene expression in embryonic stem cell aggregates using holographic optical tweezers to create biochemical gradients.

Highly versatile cell-penetrating peptide loaded scaffold for efficient and localised gene delivery to multiple cell types: From development to application in tissue engineering (2019)
Journal Article
Raftery, R. M., Walsh, D. P., Blokpoel Ferreras, L., Mencía Castaño, I., Chen, G., LeMoine, M., …O'Brien, F. J. (2019). Highly versatile cell-penetrating peptide loaded scaffold for efficient and localised gene delivery to multiple cell types: From development to application in tissue engineering. Biomaterials, 216, doi:10.1016/j.biomaterials.2019.119277

Gene therapy has recently come of age with seven viral vector-based therapies gaining regulatory approval in recent years. In tissue engineering, non-viral vectors are preferred over viral vectors, however, lower transfection efficiencies and difficu... Read More about Highly versatile cell-penetrating peptide loaded scaffold for efficient and localised gene delivery to multiple cell types: From development to application in tissue engineering.

Overall survival in malignant glioma is significantly prolonged by neurosurgical delivery of etoposide and temozolomide from a thermo-responsive biodegradable paste (2019)
Journal Article
Smith, S. J., Tyler, B., Gould, T. W., Veal, G. J., Gorelick, N. L., Rowlinson, J., …Rahman, R. (2019). Overall survival in malignant glioma is significantly prolonged by neurosurgical delivery of etoposide and temozolomide from a thermo-responsive biodegradable paste. Clinical Cancer Research, 25(16), 5094-5106. doi:10.1158/1078-0432.ccr-18-3850

Purpose: High-grade glioma (HGG) treatment is limited by the inability of otherwise potentially efficacious drugs to penetrate the blood brain barrier. We evaluate the unique intra-cavity delivery mode and translational potential of a blend of poly(D... Read More about Overall survival in malignant glioma is significantly prolonged by neurosurgical delivery of etoposide and temozolomide from a thermo-responsive biodegradable paste.

3D printed scaffolds with controlled micro-/nano- porous surface topography direct chondrogenic and osteogenic differentiation of mesenchymal stem cells (2019)
Journal Article
Prasopthum, A., Cooper, M., Shakesheff, K. M., & Yang, J. (2019). 3D printed scaffolds with controlled micro-/nano- porous surface topography direct chondrogenic and osteogenic differentiation of mesenchymal stem cells. ACS Applied Materials and Interfaces, 11(21), 18896-18906. doi:10.1021/acsami.9b01472

The effect of topography in 3D printed polymer scaffolds on stem cell differentiation is a significantly under-explored area. Compared to 2D biomaterials on which various well-defined topographies have been incorporated and been shown to direct an ar... Read More about 3D printed scaffolds with controlled micro-/nano- porous surface topography direct chondrogenic and osteogenic differentiation of mesenchymal stem cells.

Microparticles for controlled GDF6 delivery to direct ASC‐based nucleus pulposus regeneration (2019)
Journal Article
Hodgkinson, T., Stening, J. Z., White, L. J., Shakesheff, K. M., Hoyland, J. A., & Richardson, S. M. (2019). Microparticles for controlled GDF6 delivery to direct ASC‐based nucleus pulposus regeneration. Journal of Tissue Engineering and Regenerative Medicine, 13(8), 1406-1417. doi:10.1002/term.2882

Currently, there is no effective long-term treatment for intervertebral disc degeneration (IVDD), making it an attractive candidate for regenerative therapies. Hydrogel delivery of adipose stem cells (ASCs) in combination with controlled release of b... Read More about Microparticles for controlled GDF6 delivery to direct ASC‐based nucleus pulposus regeneration.

Targeted protein delivery: carbodiimide crosslinking influences protein release from microparticles incorporated within collagen scaffolds (2019)
Journal Article
Tanase, C. E., Qutachi, O., White, L. J., Shakesheff, K. M., McCaskie, A. W., Best, S. M., & Cameron, R. E. (2019). Targeted protein delivery: carbodiimide crosslinking influences protein release from microparticles incorporated within collagen scaffolds. Regenerative Biomaterials, doi:10.1093/rb/rbz015

Tissue engineering response may be tailored via controlled, sustained release of active agents from protein loaded degradable microparticles incorporated directly within 3D ice-templated collagen scaffolds. However, the effects of covalent crosslink... Read More about Targeted protein delivery: carbodiimide crosslinking influences protein release from microparticles incorporated within collagen scaffolds.

PEGylated enhanced cell penetrating peptide nanoparticles for lung gene therapy (2018)
Journal Article
Osman, G., Rodriguez, J., Chan, S. Y., Chisholm, J., Duncan, G., Kim, N., …Dixon, J. E. (2018). PEGylated enhanced cell penetrating peptide nanoparticles for lung gene therapy. Journal of Controlled Release, 285, (35-45). doi:10.1016/j.jconrel.2018.07.001. ISSN 0168-3659

The lung remains an attractive target for the gene therapy of monogenetic diseases such as cystic fibrosis (CF). Despite over 27 clinical trials, there are still very few gene therapy vectors that have shown any improvement in lung function; highligh... Read More about PEGylated enhanced cell penetrating peptide nanoparticles for lung gene therapy.

Improved delivery of PLGA microparticles and microparticle-cell scaffolds in clinical needle gauges using modified viscosity formulations (2018)
Journal Article
Qutachi, O., Wright, E. J., Bray, G., Hamid, O. A., Rose, F. R., Shakesheff, K., & Delcassian, D. (2018). Improved delivery of PLGA microparticles and microparticle-cell scaffolds in clinical needle gauges using modified viscosity formulations. International Journal of Pharmaceutics, 546(1-2), (272-278). doi:10.1016/j.ijpharm.2018.05.025. ISSN 0378-5173

Polymer microparticles are widely used as acellular drug delivery platforms in regenerative medicine, and have emerging potential as cellular scaffolds for therapeutic cell delivery. In the clinic, PLGA microparticles are typically administered intra... Read More about Improved delivery of PLGA microparticles and microparticle-cell scaffolds in clinical needle gauges using modified viscosity formulations.

A biomaterials approach to influence stem cell fate in injectable cell-based therapies (2018)
Journal Article
Amer, M. H., Rose, F. R., Shakesheff, K. M., & White, L. J. (2018). A biomaterials approach to influence stem cell fate in injectable cell-based therapies. Stem Cell Research and Therapy, 9(39), https://doi.org/10.1186/s13287-018-0789-1

Background: Numerous stem cell therapies use injection-based administration to deliver high density cell preparations. However, cell retention rates as low as 1% have been observed within days of transplantation. This study investigated the effects o... Read More about A biomaterials approach to influence stem cell fate in injectable cell-based therapies.

Post-modified polypeptides with UCST-type behavior for control of cell attachment in physiological conditions (2018)
Journal Article
Xue, X., Thiagarajan, L., Dixon, J. E., Saunders, B. R., Shakesheff, K. M., & Alexander, C. (2018). Post-modified polypeptides with UCST-type behavior for control of cell attachment in physiological conditions. Materials, 11(1), doi:10.3390/ma11010095

Upper Critical Solution Temperature (UCST)-type thermally responsive polypeptides (TRPs) with phase transition temperatures around 37 ◦C in phosphate-buffered saline (PBS) buffer (pH 7.4, 100 mM) were prepared from poly(L-ornithine) hydrobromide and... Read More about Post-modified polypeptides with UCST-type behavior for control of cell attachment in physiological conditions.

Direct three-dimensional printing of polymeric scaffolds with nanofibrous topography (2017)
Journal Article
Yang, J., Shakesheff, K. M., & Prasopthum, A. (2018). Direct three-dimensional printing of polymeric scaffolds with nanofibrous topography. Biofabrication, 10(2), doi:10.1088/1758-5090/aaa15b

Three-dimensional (3D) printing is a powerful manufacturing tool for making 3D structures with well-defined architectures for a wide range of applications. The field of tissue engineering has also adopted this technology to fabricate scaffolds for ti... Read More about Direct three-dimensional printing of polymeric scaffolds with nanofibrous topography.

Translational considerations in injectable cell-based therapeutics for neurological applications: concepts, progress and challenges (2017)
Journal Article
Amer, M. H., Rose, F. R., Shakesheff, K. M., Modo, M., & White, L. J. (in press). Translational considerations in injectable cell-based therapeutics for neurological applications: concepts, progress and challenges. npj Regenerative Medicine, 2, doi:10.1038/s41536-017-0028-x

Significant progress has been made during the past decade towards the clinical adoption of cell- based therapeutics. However, existing cell delivery approaches have shown limited success, with numerous studies showing fewer than 5% of injected cells... Read More about Translational considerations in injectable cell-based therapeutics for neurological applications: concepts, progress and challenges.

Upper critical solution temperature thermo-responsive polymer brushes and a mechanism for controlled cell attachment (2017)
Journal Article
Xue, X., Thiagarajan, L., Braim, S., Saunders, B. R., Shakesheff, K. M., & Alexander, C. (2017). Upper critical solution temperature thermo-responsive polymer brushes and a mechanism for controlled cell attachment. Journal of Materials Chemistry B, 5(25), doi:10.1039/C7TB00052A

We report the synthesis of thermo-responsive polymer brushes with Upper Critical Solution Temperature (UCST)-type behaviour on glass to provide a new means to control cell attachment. Thermoresponsive poly(N-acryloyl glycinamide)-stat-poly(N-phenylac... Read More about Upper critical solution temperature thermo-responsive polymer brushes and a mechanism for controlled cell attachment.

Decellularized bone extracellular matrix and human dental pulp stem cells as a construct for bone regeneration (2017)
Journal Article
Paduano, F., Marrelli, M., Alom, N., Amer, M. H., White, L. J., Shakesheff, K. M., & Tatullo, M. (in press). Decellularized bone extracellular matrix and human dental pulp stem cells as a construct for bone regeneration. Journal of Biomaterials Science, Polymer Edition, 28(8), doi:10.1080/09205063.2017.1301770

Dental pulp tissue represents a source of mesenchymal stem cells (MSCs) that have a strong differentiation potential towards the osteogenic lineage. The objective of the current study was to examine in vitro osteogenic induction of dental pulp stem c... Read More about Decellularized bone extracellular matrix and human dental pulp stem cells as a construct for bone regeneration.

Bioprinting using mechanically robust core-shell cell-laden hydrogel strands (2017)
Journal Article
Mistry, P., Aied, A., Alexander, M. R., Shakesheff, K., Bennett, A., & Yang, J. (in press). Bioprinting using mechanically robust core-shell cell-laden hydrogel strands. Macromolecular Bioscience, doi:10.1002/mabi.201600472

The strand material in extrusion-based bioprinting determines the microenvironments of the embedded cells and the initial mechanical properties of the constructs. One unmet challenge is the combination of optimal biological and mechanical properties... Read More about Bioprinting using mechanically robust core-shell cell-laden hydrogel strands.

Surface modification of PdlLGA microspheres with gelatine methacrylate: evaluation of adsorption, entrapment, and oxygen plasma treatment approaches (2017)
Journal Article
Baki, A., Rahman, C. V., White, L. J., Scurr, D. J., Qutachi, O., & Shakesheff, K. M. (2017). Surface modification of PdlLGA microspheres with gelatine methacrylate: evaluation of adsorption, entrapment, and oxygen plasma treatment approaches. Acta Biomaterialia, 53, 450-459. https://doi.org/10.1016/j.actbio.2017.01.042

Injectable poly (dl-lactic-co-glycolic acid) (PdlLGA) microspheres are promising candidates as biodegradable controlled release carriers for drug and cell delivery applications; however, they have limited functional groups on the surface to enable de... Read More about Surface modification of PdlLGA microspheres with gelatine methacrylate: evaluation of adsorption, entrapment, and oxygen plasma treatment approaches.

Extracellular matrix-derived hydrogels for dental stem cell delivery (2016)
Journal Article
Viswanath, A., Vanacker, J., Germain, L., Leprince, J. G., Diogenes, A., Shakesheff, K. M., …des Rieux, A. (2016). Extracellular matrix-derived hydrogels for dental stem cell delivery. Journal of Biomedical Materials Research Part A, 105(1), doi:10.1002/jbm.a.35901

Decellularised mammalian extracellular matrices (ECM) have been widely accepted as an ideal substrate for repair and remodelling of numerous tissues in clinical and pre-clinical studies. Recent studies have demonstrated the ability of ECM scaffolds d... Read More about Extracellular matrix-derived hydrogels for dental stem cell delivery.

Highly efficient intracellular transduction in three-dimensional gradients for programming cell fate (2016)
Journal Article
Eltaher, H. M., Yang, J., Shakesheff, K. M., & Dixon, J. E. (2016). Highly efficient intracellular transduction in three-dimensional gradients for programming cell fate. Acta Biomaterialia, 41, doi:10.1016/j.actbio.2016.06.004

Fundamental behaviour such as cell fate, growth and death are mediated through the control of key genetic transcriptional regulators. These regulators are activated or repressed by the integration of multiple signalling molecules in spatio-temporal g... Read More about Highly efficient intracellular transduction in three-dimensional gradients for programming cell fate.

A detailed assessment of varying ejection rate on delivery efficiency of mesenchymal stem cells using narrow-bore needles (2016)
Journal Article
Rose, F., Shakesheff, K., White, L., & Amer, M. (2016). A detailed assessment of varying ejection rate on delivery efficiency of mesenchymal stem cells using narrow-bore needles. Stem Cells Translational Medicine, 5(3), 366-378. doi:10.5966/sctm.2015-0208

As the number of clinical trials exploring cell therapy rises, a thorough understanding of the limits of cell delivery is essential. We used an extensive toolset comprising various standard and multiplex assays for the assessment of cell delivery pos... Read More about A detailed assessment of varying ejection rate on delivery efficiency of mesenchymal stem cells using narrow-bore needles.