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LC-MS metabolomics comparisons of cancer cell and macrophage responses to methotrexate and polymer- encapsulated methotrexate (2019)
Journal Article
Al-Natour, M. A., Alazzo, A., Ghaemmaghami, A. M., Kim, D., & Alexander, C. (2019). LC-MS metabolomics comparisons of cancer cell and macrophage responses to methotrexate and polymer- encapsulated methotrexate. International Journal of Pharmaceutics: X, https://doi.org/10.1016/j.ijpx.2019.100036

Methotrexate (MTX) is a folate analogue antimetabolite widely used for the treatment of rheumatoid arthritis and cancer. A number of studies have shown that MTX delivered via nanoparticle carriers is more potent against cancer cells than free MTX, a... Read More about LC-MS metabolomics comparisons of cancer cell and macrophage responses to methotrexate and polymer- encapsulated methotrexate.

A composite Gelatin/hyaluronic acid hydrogel as an ECM mimic for developing mesenchymal stem cell derived epithelial tissue patches (2019)
Journal Article
Kumar, P., Ciftci, S., Barthes, J., Knopf-Marques, H., Muller, C. B., Debry, C., …Ghaemmaghami, A. M. (2019). A composite Gelatin/hyaluronic acid hydrogel as an ECM mimic for developing mesenchymal stem cell derived epithelial tissue patches. Journal of Tissue Engineering and Regenerative Medicine, https://doi.org/10.1002/term.2962

Here we report fabrication of Gelatin based biocomposite films and their application in developing epithelial patches. The films were loaded with an epithelial cell growth factor cocktail and used as an extracellular matrix mimic (ECM mimic) for in v... Read More about A composite Gelatin/hyaluronic acid hydrogel as an ECM mimic for developing mesenchymal stem cell derived epithelial tissue patches.

The cross-talk between miR-511-3p and c-type lectin receptors on dendritic cells affects dendritic cell function (2019)
Journal Article
Awuah, D., Alobaid, M., Latif, A., Salazar, F., Emes, R. D., & Ghaemmaghami, A. M. (2019). The cross-talk between miR-511-3p and c-type lectin receptors on dendritic cells affects dendritic cell function. Journal of Immunology, 203(1), 148-157. doi:10.4049/jimmunol.1801108

MicroRNAs are small, noncoding RNAs that function as posttranscriptional modulators of gene expression by binding target mRNAs and inhibiting translation. They are therefore crucial regulators of several biological as well as immunological events. Re... Read More about The cross-talk between miR-511-3p and c-type lectin receptors on dendritic cells affects dendritic cell function.

Nanofibrous scaffolds support a 3D in vitro permeability model of the human intestinal epitheleum (2019)
Journal Article
Patient, J., Hajiali, H., Harris, K., Abrahamsson, B., Tannergreen, C., White, L., …Rose, F. (2019). Nanofibrous scaffolds support a 3D in vitro permeability model of the human intestinal epitheleum. Frontiers in Pharmacology, 10, doi:10.3389/fphar.2019.00456

Advances in drug research not only depend on high throughput screening to evaluate large numbers of lead compounds but also on the development of in vitro models which can simulate human tissues in terms of drug permeability and functions. Potential... Read More about Nanofibrous scaffolds support a 3D in vitro permeability model of the human intestinal epitheleum.

Investigating the intracellular effects of hyperbranched polycation-DNA complexes on lung cancer cells using LC-MS-based metabolite profiling (2019)
Journal Article
Alazzo, A., Al-Natour, M., Spriggs, K., Stolnik, S., Ghaemmaghami, A., Kim, D., & Alexander, C. (2019). Investigating the intracellular effects of hyperbranched polycation-DNA complexes on lung cancer cells using LC-MS-based metabolite profiling. Molecular Omics, 15(1), 77-87. doi:10.1039/C8MO00139A

Cationic polymers have emerged as a promising alternative to viral vectors in gene therapy. They are cheap to scale up, easy to functionalise and also presume to be safer than the viral vectors, however many of them are cytotoxic. The large number of... Read More about Investigating the intracellular effects of hyperbranched polycation-DNA complexes on lung cancer cells using LC-MS-based metabolite profiling.

A foreign body response-on-a-chip platform (2019)
Journal Article
Sharifi, F., Htwe, S. S., Righi, M., Liu, H., Peietralunga, A., Yesil-Celiktas, O., …Zhang, Y. S. (2019). A foreign body response-on-a-chip platform. Advanced Healthcare Materials, 8(4), 1-10. doi:10.1002/adhm.201801425

Understanding the foreign body response (FBR) and desiging strategies to modulate such a response represent a grand challenge for implant devices and biomaterials. Here, the development of a microfluidic platform is reported, i.e., the FBR‐on‐a‐chip... Read More about A foreign body response-on-a-chip platform.

New generation of bioreactors that advance extracellular matrix modelling and tissue engineering (2018)
Journal Article
Ahmed, S., Chauhan, V. M., Ghaemmaghami, A. M., & Aylott, J. W. (2019). New generation of bioreactors that advance extracellular matrix modelling and tissue engineering. Biotechnology Letters, 41(1), 1–25. doi:10.1007/s10529-018-2611-7

Bioreactors hold a lot of promise for tissue engineering and regenerative medicine applications. They have multiple uses including cell cultivation for therapeutic production and for in vitro organ modelling to provide a more physiologically relevant... Read More about New generation of bioreactors that advance extracellular matrix modelling and tissue engineering.

Photocrosslinkable gelatin hydrogels modulate the production of the major pro-inflammatory cytokine, TNF-α, by human mononuclear cells (2018)
Journal Article
Donaldson, A. R., Edi Tanase, C., Awuah, D., Vasanthi Bathri Narayanan, P., Hall, L., Nikkhah, M., …Ghaemmaghami, A. M. (2018). Photocrosslinkable gelatin hydrogels modulate the production of the major pro-inflammatory cytokine, TNF-α, by human mononuclear cells. Frontiers in Bioengineering and Biotechnology, 6, doi:10.3389/fbioe.2018.00116. ISSN 2296-4185

Hydrogels are an attractive class of biomaterials in tissue engineering due to their inherently compatible properties for cell culture. Gelatin methacryloyl (GelMA) has shown significant promise in the fields of tissue engineering and drug delivery,... Read More about Photocrosslinkable gelatin hydrogels modulate the production of the major pro-inflammatory cytokine, TNF-α, by human mononuclear cells.

Holographic optical trapping Raman micro-spectroscopy for non-invasive measurement and manipulation of live cells (2018)
Journal Article
Sinjab, F., Awuah, D., Gibson, G., Padgett, M., Ghaemmaghami, A. M., & Notingher, I. (2018). Holographic optical trapping Raman micro-spectroscopy for non-invasive measurement and manipulation of live cells. Optics Express, 26(19), https://doi.org/10.1364/oe.26.025211

We present a new approach for combining holographic optical tweezers with confocal Raman spectroscopy. Multiple laser foci, generated using a liquid-crystal spatial light modulator, are individually used for both optical trapping and excitation of sp... Read More about Holographic optical trapping Raman micro-spectroscopy for non-invasive measurement and manipulation of live cells.

Human dendritic cell sequestration onto the Necator americanus larval sheath during ex-sheathing: a possible mechanism for immune privilege (2018)
Journal Article
Hassan, A., Pritchard, D. I., & Ghaemmaghami, A. M. (2018). Human dendritic cell sequestration onto the Necator americanus larval sheath during ex-sheathing: a possible mechanism for immune privilege. Parasitology, 145(9), 1183-1190. https://doi.org/10.1017/S0031182018000136

Despite the profound health implications of Necator americanus (N. americanus) infection in humans, many aspects of its interaction with the host immune system are poorly understood. Here we investigated the early events at the interface of N. americ... Read More about Human dendritic cell sequestration onto the Necator americanus larval sheath during ex-sheathing: a possible mechanism for immune privilege.

Integrin-mediated interactions control macrophage polarization in 3D hydrogels (2017)
Journal Article
Cha, B., Shin, S. R., Leijten, J., Li, Y., Singh, S., Liu, J. C., …Khademhosseini, A. (2017). Integrin-mediated interactions control macrophage polarization in 3D hydrogels. Advanced Healthcare Materials, 6(21), https://doi.org/10.1002/adhm.201700289

Adverse immune reactions prevent clinical translation of numerous implantable devices and materials. Although inflammation is an essential part of tissue regeneration, chronic inflammation ultimately leads to implant failure. In particular, macrophag... Read More about Integrin-mediated interactions control macrophage polarization in 3D hydrogels.

Image based machine learning for identification of macrophage subsets (2017)
Journal Article
Rostam, H., Reynolds, P. M., Alexander, M. R., Gadegaard, N., & Ghaemmaghami, A. M. (in press). Image based machine learning for identification of macrophage subsets. Scientific Reports, 7, https://doi.org/10.1038/s41598-017-03780-z

Macrophages play a crucial rule in orchestrating immune responses against pathogens and foreign materials. Macrophages have remarkable plasticity in response to environmental cues and are able to acquire a spectrum of activation status, best exemplif... Read More about Image based machine learning for identification of macrophage subsets.

Incorporation of resident macrophages in engineered tissues: Multiple cell type response to microenvironment controlled macrophage-laden gelatine hydrogels (2017)
Journal Article
Dollinger, C., Ciftci, S., Knopf-Marques, H., Ghaemmaghami, A. M., Debry, C., Barthes, J., & Vrana, N. E. (2018). Incorporation of resident macrophages in engineered tissues: Multiple cell type response to microenvironment controlled macrophage-laden gelatine hydrogels. Journal of Tissue Engineering and Regenerative Medicine, 12(2), 330-340. https://doi.org/10.1002/term.2458

The success of tissue engineering strategy is strongly related to the inflammatory response, mainly through the activity of macrophages that are key cells in initial immune response to implants. For engineered tissues, the presence of resident macrop... Read More about Incorporation of resident macrophages in engineered tissues: Multiple cell type response to microenvironment controlled macrophage-laden gelatine hydrogels.

Unbiased analysis of the impact of micropatterned biomaterials on macrophage behaviour provides insights beyond pre-defined polarisation states (2017)
Journal Article
Singh, S., Awuah, D., Rostam, H., Emes, R., Kandola, N. K., Onion, D., …Ghaemmaghami, A. M. (in press). Unbiased analysis of the impact of micropatterned biomaterials on macrophage behaviour provides insights beyond pre-defined polarisation states. ACS Biomaterials Science and Engineering, doi:10.1021/acsbiomaterials.7b00104

Macrophages are master regulators of immune responses towards implanted biomaterials. The activation state adopted by macrophages in response to biomaterials determines their own phenotype and function as well as those of other resident and infiltrat... Read More about Unbiased analysis of the impact of micropatterned biomaterials on macrophage behaviour provides insights beyond pre-defined polarisation states.

The role of indoleamine 2,3-dioxygenase-aryl hydrocarbon receptor pathway in the TLR4-induced tolerogenic phenotype in human DCs (2017)
Journal Article
Salazar, F., Awuah, D., Negm, O. H., Shakib, F., & Ghaemmaghami, A. M. (2017). The role of indoleamine 2,3-dioxygenase-aryl hydrocarbon receptor pathway in the TLR4-induced tolerogenic phenotype in human DCs. Scientific Reports, 7(1), https://doi.org/10.1038/srep43337

A controlled inflammatory response is required for protection against infection, but persistent inflammation causes tissue damage. Dendritic cells (DCs) have a unique capacity to promote both inflammatory and anti-inflammatory processes. One key mech... Read More about The role of indoleamine 2,3-dioxygenase-aryl hydrocarbon receptor pathway in the TLR4-induced tolerogenic phenotype in human DCs.

Role of ROCK isoforms in regulation of stiffness induced myofibroblast differentiation in lung fibrosis (2017)
Journal Article
Htwe, S. S., Cha, B. H., Yue, K., Khademhosseini, A., Knox, A. J., & Ghaemmaghami, A. M. (2017). Role of ROCK isoforms in regulation of stiffness induced myofibroblast differentiation in lung fibrosis. American Journal of Respiratory Cell and Molecular Biology, 56(6), 772-783. https://doi.org/10.1165/rcmb.2016-0306OC

Fibrosis is a major cause of progressive organ dysfunction in several chronic pulmonary diseases. Rho associated coiled-coil forming kinase (ROCK) has shown to be involved in myofibroblast differentiation driven by altered matrix stiffness in fibroti... Read More about Role of ROCK isoforms in regulation of stiffness induced myofibroblast differentiation in lung fibrosis.

Generation of anti-inflammatory macrophages for implants and regenerative medicine using self-standing release systems with a phenotype-fixing cytokine cocktail formulation (2017)
Journal Article
Riabov, V., Salazar, F., Htwe, S. S., Gudima, A., Schmuttermaier, C., Barthes, J., …Kzhyshkowska, J. (2017). Generation of anti-inflammatory macrophages for implants and regenerative medicine using self-standing release systems with a phenotype-fixing cytokine cocktail formulation. Acta Biomaterialia, 53, 389-398. https://doi.org/10.1016/j.actbio.2017.01.071

The immediate tissue microenvironment of implanted biomedical devices and engineered tissues is highly influential on their long term fate and efficacy. The creation of a long-term anti-inflammatory microenvironment around implants and artificial tis... Read More about Generation of anti-inflammatory macrophages for implants and regenerative medicine using self-standing release systems with a phenotype-fixing cytokine cocktail formulation.

Electrohydrodynamic printing as a method to micropattern large titanium implant surfaces with photocrosslinkable structures (2017)
Journal Article
Pokorny, M., Klemes, J., Zidek, O., Dollinger, C., Ozcebe, G., Singh, S., …Vrana, N. E. (in press). Electrohydrodynamic printing as a method to micropattern large titanium implant surfaces with photocrosslinkable structures. Biomedical Physics and Engineering Express, 3(1), doi:10.1088/2057-1976/3/1/015002

Metallic implants are widely used in orthopaedic and orthodontic applications. However, generally surface treatment of the metallic surfaces is necessary to render them more biologically active. Herein, we describe a direct write printing method to m... Read More about Electrohydrodynamic printing as a method to micropattern large titanium implant surfaces with photocrosslinkable structures.

A paper-based in vitro model for on-chip investigation of the human respiratory system (2016)
Journal Article
Rahimi, R., Htwe, S. S., Ochoa, M., Donaldson, A., Zieger, M., Sood, R., …Ziaie, B. (in press). A paper-based in vitro model for on-chip investigation of the human respiratory system. Lab on a Chip, 16(22), doi:10.1039/C6LC00866F

Culturing cells at the air–liquid interface (ALI) is essential for creating functional in vitro models of lung tissues. We present the use of direct-patterned laser-treated hydrophobic paper as an effective semi-permeable membrane, ideal for ALI cell... Read More about A paper-based in vitro model for on-chip investigation of the human respiratory system.

Effective antigen presentation to helper T cells by human eosinophils (2016)
Journal Article
Farhan, R. K., Vickers, M. A., Ghaemmaghami, A. M., Hall, A. M., Barker, R. N., & Walsh, G. M. (in press). Effective antigen presentation to helper T cells by human eosinophils. Immunology, doi:10.1111/imm.12658

Although eosinophils are inflammatory cells, there is increasing attention on their immunomodulatory roles. For example, murine eosinophils can present antigen to CD4+ T helper (Th) cells, but it remains unclear whether human eosinophils also have th... Read More about Effective antigen presentation to helper T cells by human eosinophils.