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Synthesis of methacrylate-terminated block copolymers with reduced transesterification by controlled ring-opening polymerization (2018)
Journal Article
Ruiz-Cantu, L. A., Pearce, A. K., Burroughs, L., Bennett, T. M., Vasey, C. E., Wildman, R., …Taresco, V. (2019). Synthesis of methacrylate-terminated block copolymers with reduced transesterification by controlled ring-opening polymerization. Macromolecular Chemistry and Physics, 220(2), Article 1800459. https://doi.org/10.1002/macp.201800459

This work presents a robust method to achieve the synthesis of low molecular weight polyesters 26 via ring-opening polymerization (ROP) initiated by 2-hydroxyethyl-methacrylate (HEMA) 27 when using triazabicyclodecene (TBD) as catalyst. The effect th... Read More about Synthesis of methacrylate-terminated block copolymers with reduced transesterification by controlled ring-opening polymerization.

Molecular Simulations of Ion Effects on the Thermodynamics of RNA Folding (2018)
Journal Article
Denesyuk, N. A., Hori, N., & Thirumalai, D. (2018). Molecular Simulations of Ion Effects on the Thermodynamics of RNA Folding. Journal of Physical Chemistry B, 122(50), 11860-11867. https://doi.org/10.1021/acs.jpcb.8b08142

How ions affect RNA folding thermodynamics and kinetics is an important but a vexing problem that remains unsolved. Experiments have shown that the free-energy change, δG(c), of RNA upon folding varies with the salt concentration (c) as, δG(c) = k c... Read More about Molecular Simulations of Ion Effects on the Thermodynamics of RNA Folding.

Measurement of the total angiotensinogen and its reduced and oxidised forms in human plasma using targeted LC-MS/MS (2018)
Journal Article
Dahabiyeh, L. A., Tooth, D., Carrell, R. W., Read, R. J., Yan, Y., Pipkin, F. B., & Barrett, D. A. (2019). Measurement of the total angiotensinogen and its reduced and oxidised forms in human plasma using targeted LC-MS/MS. Analytical and Bioanalytical Chemistry, 411(2), 427–437. https://doi.org/10.1007/s00216-018-1455-2

Angiotensinogen (AGT) is a critical protein in the renin-angiotensin-aldosterone system and may have an important role in the pathogenesis of pre-eclampsia. The disulphide linkage between cysteines 18 and 138 has a key role in the redox switch of AGT... Read More about Measurement of the total angiotensinogen and its reduced and oxidised forms in human plasma using targeted LC-MS/MS.

The central region of CNOT1 and CNOT9 stimulate deadenylation by the Ccr4 Not nuclease module (2018)
Journal Article
Pavanello, L., Hall, B., Airhihen, B., & Winkler, G. S. (2018). The central region of CNOT1 and CNOT9 stimulate deadenylation by the Ccr4 Not nuclease module. Biochemical Journal, 475(21), 3437–3450. https://doi.org/10.1042/bcj20180456

Regulated degradation of cytoplasmic mRNA is important for the accurate execution of gene expression programmes in eukaryotic cells. A key step in this process is the shortening and removal of the mRNA poly(A) tail, which can be achieved by the recru... Read More about The central region of CNOT1 and CNOT9 stimulate deadenylation by the Ccr4 Not nuclease module.

Rapid and accurate analysis of stem cell-derived extracellular vesicles with super resolution microscopy and live imaging (2018)
Journal Article
Nizamudeen, Z., Markus, R., Lodge, R., Parmenter, C., Platt, M., Chakrabarti, L., & Sottile, V. (2018). Rapid and accurate analysis of stem cell-derived extracellular vesicles with super resolution microscopy and live imaging. Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1865(12), 1891-1900. https://doi.org/10.1016/j.bbamcr.2018.09.008

Extracellular vesicles (EVs) have prevalent roles in cancer biology and regenerative medicine. Conventional techniques for characterising EVs including electron microscopy (EM), nanoparticle tracking analysis (NTA) and tuneable resistive pulse sensin... Read More about Rapid and accurate analysis of stem cell-derived extracellular vesicles with super resolution microscopy and live imaging.

Photocrosslinkable gelatin hydrogels modulate the production of the major pro-inflammatory cytokine, TNF-α, by human mononuclear cells (2018)
Journal Article
Donaldson, A. R., Edi Tanase, C., Awuah, D., Vasanthi Bathri Narayanan, P., Hall, L., Nikkhah, M., …Ghaemmaghami, A. M. (2018). Photocrosslinkable gelatin hydrogels modulate the production of the major pro-inflammatory cytokine, TNF-α, by human mononuclear cells. Frontiers in Bioengineering and Biotechnology, 6, Article 116. https://doi.org/10.3389/fbioe.2018.00116

Hydrogels are an attractive class of biomaterials in tissue engineering due to their inherently compatible properties for cell culture. Gelatin methacryloyl (GelMA) has shown significant promise in the fields of tissue engineering and drug delivery,... Read More about Photocrosslinkable gelatin hydrogels modulate the production of the major pro-inflammatory cytokine, TNF-α, by human mononuclear cells.

Analytical ultracentrifugation in saliva research: Impact of green tea astringency and its significance on the in-vivo aroma release (2018)
Journal Article
Dinu, V., Liu, C., Ali, J., Ayed, C., Gershkovich, P., Adams, G. G., …Fisk, I. (2018). Analytical ultracentrifugation in saliva research: Impact of green tea astringency and its significance on the in-vivo aroma release. Scientific Reports, 8, 1-9. https://doi.org/10.1038/s41598-018-31625-w

Current saliva testing methods rely on cutting edge yet expensive techniques for the detection and analysis of genetic material, proteins and biomarkers for clinical use. However, these techniques are limited in scope and often cannot be used with co... Read More about Analytical ultracentrifugation in saliva research: Impact of green tea astringency and its significance on the in-vivo aroma release.

Autophagy modulation: a prudent approach in cancer treatment? (2018)
Journal Article
Bishop, E., & Bradshaw, T. D. (2018). Autophagy modulation: a prudent approach in cancer treatment?. Cancer Chemotherapy and Pharmacology, https://doi.org/10.1007/s00280-018-3669-6

Autophagy is a tightly controlled process comprising lysosomal degradation and recycling of cellular proteins and organelles. In cancer, its paradoxical dual role of cytoprotection and cytotoxicity is context-dependent and controversial. Autophagy pr... Read More about Autophagy modulation: a prudent approach in cancer treatment?.

Shear bands and the evolving microstructure in a drying colloidal film studied with scanning μ-SAXS (2018)
Journal Article
Yang, B., Smith, N. D., Johannes, A., Burghammer, M., & Smith, M. I. (2018). Shear bands and the evolving microstructure in a drying colloidal film studied with scanning μ-SAXS. Scientific Reports, 8, Article 12979. https://doi.org/10.1038/s41598-018-31405-6

Shear localisation in thin bands is an important process involved in the plastic deformation of materials subject to stress. This process is often sensitive to the sample microstructure (amorphous / crystalline). Here we show using the scanning μ-SAX... Read More about Shear bands and the evolving microstructure in a drying colloidal film studied with scanning μ-SAXS.

Assessment of the cellular localisation of the annexin A2/S100A10 complex in human placenta (2018)
Journal Article
Seham, E., & Lodewijk, D. (2018). Assessment of the cellular localisation of the annexin A2/S100A10 complex in human placenta. Journal of Molecular Histology, 49(5), 531-543. https://doi.org/10.1007/s10735-018-9791-2

The AnxA2/S100A10 complex has been implicated in various placental functions but although the localisation of these proteins individually has been studied, there is no information about the localisation of their complex in situ at the cellular level.... Read More about Assessment of the cellular localisation of the annexin A2/S100A10 complex in human placenta.

Therapeutic exploitation of IPSE, a urogenital parasite-derived host modulatory protein, for chemotherapy-induced hemorrhagic cystitis (2018)
Journal Article
Mbanefo, E. C., Le, L., Pennington, L. F., Odegaard, J. I., Jardetzky, T. S., Alouffi, A., …Hsieh, M. H. (2018). Therapeutic exploitation of IPSE, a urogenital parasite-derived host modulatory protein, for chemotherapy-induced hemorrhagic cystitis. FASEB Journal, 32(8), 4408-4419. https://doi.org/10.1096/fj.201701415r

Chemotherapy-induced hemorrhagic cystitis (CHC) can be difficult to manage. Prior work suggests that IL-4 alleviates ifosfamide-induced hemorrhagic cystitis (IHC), but systemically administered IL-4 causes significant side effects. We hypothesized th... Read More about Therapeutic exploitation of IPSE, a urogenital parasite-derived host modulatory protein, for chemotherapy-induced hemorrhagic cystitis.

The Meisenheimer Complex as a Paradigm in Drug Discovery: Reversible Covalent Inhibition through C67 of the ATP Binding Site of PLK1 (2018)
Journal Article
Pearson, R. J., Blake, D. G., Mezna, M., Fischer, P. M., Westwood, N. J., & McInnes, C. (2018). The Meisenheimer Complex as a Paradigm in Drug Discovery: Reversible Covalent Inhibition through C67 of the ATP Binding Site of PLK1. Cell Chemical Biology, 25(9), 1107-1116.e4. https://doi.org/10.1016/j.chembiol.2018.06.001

The polo kinase family are important oncology targets that act in regulating entry into and progression through mitosis. Structure-guided discovery of a new class of inhibitors of Polo-like kinase 1 (PLK1) catalytic activity that interact with Cys67... Read More about The Meisenheimer Complex as a Paradigm in Drug Discovery: Reversible Covalent Inhibition through C67 of the ATP Binding Site of PLK1.

Transcriptional analysis of adipose tissue during development reveals depot-specific responsiveness to maternal dietary supplementation (2018)
Journal Article
Fainberg, H. P., Birtwistle, M., Alagal, R., Alhaddad, A., Pope, M., Davies, G., …Symonds, M. E. (2018). Transcriptional analysis of adipose tissue during development reveals depot-specific responsiveness to maternal dietary supplementation. Scientific Reports, 8(1), 1-11. https://doi.org/10.1038/s41598-018-27376-3

Brown adipose tissue (BAT) undergoes pronounced changes after birth coincident with the loss of the BAT-specifc uncoupling protein (UCP)1 and rapid fat growth. The extent to which this adaptation may vary between anatomical locations remains unknown,... Read More about Transcriptional analysis of adipose tissue during development reveals depot-specific responsiveness to maternal dietary supplementation.

Polyionic tags as enhancers of protein solubility in recombinant protein expression (2018)
Journal Article
Paraskevopoulou, V., & Falcone, F. (2018). Polyionic tags as enhancers of protein solubility in recombinant protein expression. Microorganisms, 6(2), 47. https://doi.org/10.3390/microorganisms6020047

Since the introduction of recombinant protein expression in the second half of the 1970s, the growth of the biopharmaceutical field has been rapid and protein therapeutics has come to the foreground. Biophysical and structural characterisation of rec... Read More about Polyionic tags as enhancers of protein solubility in recombinant protein expression.