Skip to main content

Research Repository

Advanced Search

All Outputs (5)

InFusion cloning for the generation of biologically relevant HCV chimeric molecular clones (2018)
Book Chapter
King, B., Urbanowicz, R., Tarr, A. W., Ball, J. K., & McClure, C. P. (2019). InFusion cloning for the generation of biologically relevant HCV chimeric molecular clones. In M. Law (Ed.), Hepatitis C Virus Protocols (93-104). New York: Springer. https://doi.org/10.1007/978-1-4939-8976-8_6

This chapter describes how to generate chimeric molecular cassettes that are ready to receive PCR-amplified E1/E2 genes using new DNA cloning technology. The method is divided into three sections: (1) generation of a ΔCore-NS2 cassette based upon the... Read More about InFusion cloning for the generation of biologically relevant HCV chimeric molecular clones.

Cloning and analysis of authentic patient-derived HCV E1/E2 glycoproteins (2018)
Book Chapter
Urbanowicz, R. A., Ball, J. K., & Tarr, A. W. (2019). Cloning and analysis of authentic patient-derived HCV E1/E2 glycoproteins. In M. Law (Ed.), Hepatitis C Virus Protocols, 275-294. Springer Publishing Company. doi:10.1007/978-1-4939-8976-8_19

Experimental characterization of the properties of authentic viruses circulating in infected individuals presents a problem when investigating RNA viruses with error-prone polymerases. The hepatitis C virus provides an extreme example of RNA virus ge... Read More about Cloning and analysis of authentic patient-derived HCV E1/E2 glycoproteins.

Functional and immunogenic characterization of diverse HCV glycoprotein E2 variants (2018)
Journal Article
Khera, T., Behrendt, P., Bankwitz, D., Brown, R. J., Todt, D., Doepke, M., …Pietschmann, T. (2019). Functional and immunogenic characterization of diverse HCV glycoprotein E2 variants. Journal of Hepatology, 70(4), 593-602. https://doi.org/10.1016/j.jhep.2018.11.003

© 2018 European Association for the Study of the Liver Background & Aims: Induction of cross-reactive antibodies targeting conserved epitopes of the envelope proteins E1E2 is a key requirement for an hepatitis C virus vaccine. Conserved epitopes like... Read More about Functional and immunogenic characterization of diverse HCV glycoprotein E2 variants.

Immunization with a synthetic consensus hepatitis C virus E2 glycoprotein ectodomain elicits virus-neutralizing antibodies (2018)
Journal Article
Tarr, A. W., Backx, M., Hamed, M. R., Urbanowicz, R. A., McClure, C. P., Brown, R. J., & Ball, J. K. (2018). Immunization with a synthetic consensus hepatitis C virus E2 glycoprotein ectodomain elicits virus-neutralizing antibodies. Antiviral Research, 160, 25-37. https://doi.org/10.1016/j.antiviral.2018.09.005

Global eradication of hepatitis C virus (HCV) infection will require an efficacious vaccine capable of eliciting protective immunity against genetically diverse HCV strains. Natural spontaneous resolution of HCV infection is associated with productio... Read More about Immunization with a synthetic consensus hepatitis C virus E2 glycoprotein ectodomain elicits virus-neutralizing antibodies.

Enhanced nanoparticle uptake into virus infected cells: could nanoparticles be useful in antiviral therapy? (2018)
Journal Article
Abo-zeid, Y., Urbanowicz, R. A., Thomson, B. J., Irving, W. L., Tarr, A. W., & Garnett, M. C. (2018). Enhanced nanoparticle uptake into virus infected cells: could nanoparticles be useful in antiviral therapy?. International Journal of Pharmaceutics, 547(1-2), https://doi.org/10.1016/j.ijpharm.2018.06.027

Virus infections cause diseases of different severity ranged from mild infection e.g. common cold into life threatening diseases e.g. Human Immunodeficiency virus (HIV), Hepatitis B. Virus infections represent 44% of newly emerging infections. Althou... Read More about Enhanced nanoparticle uptake into virus infected cells: could nanoparticles be useful in antiviral therapy?.