All Outputs (9)

Analytical ultracentrifugation in saliva research: Impact of green tea astringency and its significance on the in-vivo aroma release (2018)
Journal Article
Dinu, V., Liu, C., Ali, J., Ayed, C., Gershkovich, P., Adams, G. G., …Fisk, I. (2018). Analytical ultracentrifugation in saliva research: Impact of green tea astringency and its significance on the in-vivo aroma release. Scientific Reports, 8, 1-9. https://doi.org/10.1038/s41598-018-31625-w

Current saliva testing methods rely on cutting edge yet expensive techniques for the detection and analysis of genetic material, proteins and biomarkers for clinical use. However, these techniques are limited in scope and often cannot be used with co... Read More about Analytical ultracentrifugation in saliva research: Impact of green tea astringency and its significance on the in-vivo aroma release.

The discovery of hydrogen bonds in DNA and a re-evaluation of the 1948 Creeth two-chain model for its structure (2018)
Journal Article
Harding, S. E., Channell, G., & Phillips-Jones, M. K. (2018). The discovery of hydrogen bonds in DNA and a re-evaluation of the 1948 Creeth two-chain model for its structure. Biochemical Society Transactions, 46(5), 1171-1182. https://doi.org/10.1042/BST20180158

We recall the experimental approaches involved in the discovery of hydrogen bonds in deoxyribonucleic acid (DNA) made 70 years ago by a team of scientists at University College Nottingham led by J.M. Gulland, and in relation to previous studies. This... Read More about The discovery of hydrogen bonds in DNA and a re-evaluation of the 1948 Creeth two-chain model for its structure.

Use of the extended Fujita method for representing the molecular weight and molecular weight distributions of native and processed oat beta-glucans (2018)
Journal Article
Channell, G. A., Adams, G. G., Lu, Y., Gillis, R. B., Dinu, V., Grundy, M. M., …Harding, S. E. (2018). Use of the extended Fujita method for representing the molecular weight and molecular weight distributions of native and processed oat beta-glucans. Scientific Reports, 8(1), Article 11809. https://doi.org/10.1038/s41598-018-29997-0

Beta 1–3, 1–4 glucans (“beta-glucans”) are one of the key components of the cell wall of cereals, complementing the main structural component cellulose. Beta-glucans are also an important source of soluble fibre in foods containing oats with claims o... Read More about Use of the extended Fujita method for representing the molecular weight and molecular weight distributions of native and processed oat beta-glucans.

Production of membrane proteins for characterisation of their pheromone-sensing and antimicrobial resistance functions (2018)
Journal Article
Azam, A. A., Kinder, J. M., Khan, G. N., Alase, A., Ma, P., Liu, Y., …Phillips-Jones, M. K. (2018). Production of membrane proteins for characterisation of their pheromone-sensing and antimicrobial resistance functions. European Biophysics Journal, https://doi.org/10.1007/s00249-018-1325-z

Despite the importance of membrane proteins in cellular processes, studies of these hydrophobic proteins present major technical challenges, including expression and purification for structural and biophysical studies. A modified strategy of that pro... Read More about Production of membrane proteins for characterisation of their pheromone-sensing and antimicrobial resistance functions.

The Svedberg Lecture 2017. From nano to micro: the huge dynamic range of the analytical ultracentrifuge for characterizing the sizes, shapes and interactions of molecules and assemblies in biochemistry and polymer science (2018)
Journal Article
Harding, S. E., Wakefield, J., Gillis, R., Adams, G., & McQueen, C. (2018). The Svedberg Lecture 2017. From nano to micro: the huge dynamic range of the analytical ultracentrifuge for characterizing the sizes, shapes and interactions of molecules and assemblies in biochemistry and polymer science. European Biophysics Journal, 47(7), 697–707. https://doi.org/10.1007/s00249-018-1290-6

The analytical ultracentrifuge (AUC) invented by T. Svedberg has now become an extremely versatile and diverse tool in Biochemistry and Polymer Science for the characterisation of the sizes, shapes and interactions of particles ranging in size from a... Read More about The Svedberg Lecture 2017. From nano to micro: the huge dynamic range of the analytical ultracentrifuge for characterizing the sizes, shapes and interactions of molecules and assemblies in biochemistry and polymer science.

Characterisation of insulin analogues therapeutically available to patients (2018)
Journal Article
Adams, G. G., Meal, A., Morgan, P. S., Alzahrani, Q. E., Zobel, H., Lithgo, R., …Chayen, N. (2018). Characterisation of insulin analogues therapeutically available to patients. PLoS ONE, 13(3), Article e0195010. https://doi.org/10.1371/journal.pone.0195010

The structure and function of clinical dosage insulin and its analogues were assessed. This included ‘native insulins’ (human recombinant, bovine, porcine), ‘fast-acting analogues’ (aspart, glulisine, lispro) and ‘slow-acting analogues’ (glargine, de... Read More about Characterisation of insulin analogues therapeutically available to patients.

Controlled depolymerisation, as assessed by analytical ultracentrifugation, of low molecular weight chitosan for potential use in archaeological conservation (2018)
Journal Article
Wakefield, J. M., Gillis, R. B., Adams, G. G., McQueen, C., & Harding, S. E. (2018). Controlled depolymerisation, as assessed by analytical ultracentrifugation, of low molecular weight chitosan for potential use in archaeological conservation. European Biophysics Journal, 47(7), 769-775. https://doi.org/10.1007/s00249-018-1290-6

The heterogeneity and molecular weight of a chitosan of low molecular weight (molar mass) and low degree of acetylation (0.1), for potential use as a consolidant for decayed archaeological wood, has been examined by sedimentation velocity and sedimen... Read More about Controlled depolymerisation, as assessed by analytical ultracentrifugation, of low molecular weight chitosan for potential use in archaeological conservation.

Antimicrobial resistance (AMR) nanomachines—mechanisms for fluoroquinolone and glycopeptide recognition, efflux and/or deactivation (2018)
Journal Article
Phillips-Jones, M. K., & Harding, S. E. (2018). Antimicrobial resistance (AMR) nanomachines—mechanisms for fluoroquinolone and glycopeptide recognition, efflux and/or deactivation. Biophysical Reviews, 10(2), 347-362. https://doi.org/10.1007/s12551-018-0404-9

In this review, we discuss mechanisms of resistance identified in bacterial agents Staphylococcus aureus and the enterococci towards two priority classes of antibiotics—the fluoroquinolones and the glycopeptides. Members of both classes interact with... Read More about Antimicrobial resistance (AMR) nanomachines—mechanisms for fluoroquinolone and glycopeptide recognition, efflux and/or deactivation.

Stabilisation of oil-in-water emulsions with non-chemical modified gelatinised starch (2018)
Journal Article
Kasprzak, M. M., Macnaughtan, W., Harding, S., Wilde, P., & Wolf, B. (2018). Stabilisation of oil-in-water emulsions with non-chemical modified gelatinised starch. Food Hydrocolloids, 81, https://doi.org/10.1016/j.foodhyd.2018.03.002

In this research, stabilisation of oil-in-water emulsions with non-chemically modified gelatinised starch is presented. Thus far only octenyl succinic anhydride(OSA) modified gelatinised starch has been known to adsorb at emulsion droplet interfaces,... Read More about Stabilisation of oil-in-water emulsions with non-chemical modified gelatinised starch.