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CRISPR-Cas immunity, DNA repair and genome stability (2018)
Journal Article
Cubbon, A., Ivancic-Bace, I., & Bolt, E. (2018). CRISPR-Cas immunity, DNA repair and genome stability. Bioscience Reports, 38(5), 1-10. https://doi.org/10.1042/bsr20180457

© 2018 The Author(s). Co-opting of CRISPR-Cas ‘Interference’ reactions for editing the genomes of eukaryotic and prokaryotic cells has highlighted crucial support roles for DNA repair systems that strive to maintain genome stability. As front-runners... Read More about CRISPR-Cas immunity, DNA repair and genome stability.

CRISPR-Cas adaptation in Escherichia coli requires RecBCD helicase but not nuclease activity, is independent of homologous recombination, and is antagonized by 5' ssDNA exonucleases (2018)
Journal Article
Radovčić, M., Killelea, T., Savitskaya, E., Wettstein, L., Bolt, E. L., & Ivančić-Baće, I. (2018). CRISPR-Cas adaptation in Escherichia coli requires RecBCD helicase but not nuclease activity, is independent of homologous recombination, and is antagonized by 5' ssDNA exonucleases. Nucleic Acids Research, 46(19), 10173-10183. https://doi.org/10.1093/nar/gky799

© The Author(s) 2018. Prokaryotic adaptive immunity is established against mobile genetic elements (MGEs) by 'naïve adaptation' when DNA fragments from a newly encountered MGE are integrated into CRISPR-Cas systems. In Escherichia coli, DNA integrati... Read More about CRISPR-Cas adaptation in Escherichia coli requires RecBCD helicase but not nuclease activity, is independent of homologous recombination, and is antagonized by 5' ssDNA exonucleases.

DNA replication roadblocks caused by Cascade interference complexes are alleviated by RecG DNA repair helicase (2018)
Journal Article
Howard, J. L., Killelea, T., Hawkins, M., Howard, J., McGlynn, P., & Bolt, E. L. (2019). DNA replication roadblocks caused by Cascade interference complexes are alleviated by RecG DNA repair helicase. RNA Biology, 16(4), 543-548. https://doi.org/10.1080/15476286.2018.1496773

Cascade complexes underpin E. coli CRISPR-Cas immunity systems by stimulating "adaptation" reactions that update immunity and by initiating "interference" reactions that destroy invader DNA. Recognition of invader DNA in Cascade catalysed R-loops pro... Read More about DNA replication roadblocks caused by Cascade interference complexes are alleviated by RecG DNA repair helicase.