Purification of bacterial membrane sensor kinases and biophysical methods for determination of their ligand and inhibitor interactions
(2016)
Journal Article
Hussain, R., Harding, S. E., Hughes, C. S., Ma, P., Patching, S. G., Edara, S., …Phillips-Jones, M. K. (2016). Purification of bacterial membrane sensor kinases and biophysical methods for determination of their ligand and inhibitor interactions. Biochemical Society Transactions, 44(3), 810-823. https://doi.org/10.1042/BST20160023
This article reviews current methods for the reliable heterologous overexpression in Escherichia coli and purification of milligram quantities of bacterial membrane sensor kinase (MSK) proteins belonging to the two-component signal transduction famil... Read More about Purification of bacterial membrane sensor kinases and biophysical methods for determination of their ligand and inhibitor interactions.