All Outputs (13)

What's a SNP between friends: The influence of single nucleotide polymorphisms on virulence and phenotypes of Clostridium difficile strain 630 and derivatives (2016)
Journal Article
Collery, M. M., Kuehne, S. A., McBride, S. M., Kelly, M. L., Monot, M., Cockayne, A., …Minton, N. P. (2017). What's a SNP between friends: The influence of single nucleotide polymorphisms on virulence and phenotypes of Clostridium difficile strain 630 and derivatives. Virulence, 8(6), 767-781. https://doi.org/10.1080/21505594.2016.1237333

Clostridium difficile is a major cause of antibiotic induced diarrhoea worldwide, responsible for significant annual mortalities and represents a considerable economic burden on healthcare systems. The two main C. difficile virulence factors are toxi... Read More about What's a SNP between friends: The influence of single nucleotide polymorphisms on virulence and phenotypes of Clostridium difficile strain 630 and derivatives.

Enhanced solvent production by metabolic engineering of a twin-clostridial consortium (2016)
Journal Article
Wen, Z., Minton, N. P., Zhang, Y., Li, Q., Liu, J., Jiang, Y., & Yang, S. (2017). Enhanced solvent production by metabolic engineering of a twin-clostridial consortium. Metabolic Engineering, 39, 38-48. doi:10.1016/j.ymben.2016.10.013

Coinfection and Emergence of Rifamycin Resistance during a Recurrent Clostridium difficile Infection (2016)
Journal Article
Stevenson, E. C., Major, G. A., Spiller, R. C., Kuehne, S. A., & Minton, N. P. (2016). Coinfection and Emergence of Rifamycin Resistance during a Recurrent Clostridium difficile Infection. Journal of Clinical Microbiology, 54(11), 2689-2694. https://doi.org/10.1128/jcm.01025-16

Clostridium difficile (Peptoclostridium difficile) is a common health care-associated infection with a disproportionately high incidence in elderly patients. Disease symptoms range from mild diarrhea to life-threatening pseudomembranous colitis. Arou... Read More about Coinfection and Emergence of Rifamycin Resistance during a Recurrent Clostridium difficile Infection.

A genetic assay for gene essentiality in Clostridium (2016)
Journal Article
Walker, D. J., Heap, J. T., Winzer, K., & Minton, N. P. (2016). A genetic assay for gene essentiality in Clostridium. Anaerobe, 42, 40-43. https://doi.org/10.1016/j.anaerobe.2016.07.007

Essential genes of pathogens are potential therapeutic targets, but are difficult to verify. Here, gene essentiality was determined by targeted knockout following engineered gene duplication. Null mutants of candidate essential genes of Clostridium d... Read More about A genetic assay for gene essentiality in Clostridium.

The binary toxin CDT enhances Clostridium difficile virulence by suppressing protective colonic eosinophilia (2016)
Journal Article
Cowardin, C. A., Buonomo, E. L., Saleh, M. M., Wilson, M. G., Burgess, S. L., Schwan, C., …Petri, W. A. (2016). The binary toxin CDT enhances Clostridium difficile virulence by suppressing protective colonic eosinophilia. Nature Microbiology, 1(8), Article 16108. https://doi.org/10.1038/nmicrobiol.2016.108

© 2016 Macmillan Publishers Limited. All rights reserved. Clostridium difficile is the most common hospital acquired pathogen in the USA, and infection is, in many cases, fatal. Toxins A and B are its major virulence factors, but expression of a thir... Read More about The binary toxin CDT enhances Clostridium difficile virulence by suppressing protective colonic eosinophilia.

SBRC-Nottingham: sustainable routes to platform chemicals from C1 wastes Gases (2016)
Journal Article
Burbidge, A., & Minton, N. P. (2016). SBRC-Nottingham: sustainable routes to platform chemicals from C1 wastes Gases. Biochemical Society Transactions, 44(3), 684-686. https://doi.org/10.1042/BST20160010

SBRC-Nottingham (www.sbrc-nottingham.ac.uk) was one of the first three UK university-based Synthetic Biology Research Centres (SBRCs) to be funded by the BBSRC and EPSRC as part of the recommendations made in the UKs Synthetic Biology Roadmap. It was... Read More about SBRC-Nottingham: sustainable routes to platform chemicals from C1 wastes Gases.

Insights into CO2 fixation pathway of Clostridium autoethanogenumby targeted mutagenesis (2016)
Journal Article
Liew, F., Henstra, A. M., Winzer, K., Kopke, M., Simpson, S. D., & Minton, N. P. (in press). Insights into CO2 fixation pathway of Clostridium autoethanogenumby targeted mutagenesis. mBio, 7(3), Article e00427-16. https://doi.org/10.1128/mBio.00427-16

The future sustainable production of chemicals and fuels from nonpetrochemical resources and reduction of greenhouse gas emissions are two of the greatest societal challenges. Gas fermentation, which utilizes the ability of acetogenic bacteria such a... Read More about Insights into CO2 fixation pathway of Clostridium autoethanogenumby targeted mutagenesis.

A roadmap for gene system development in Clostridium (2016)
Journal Article
Minton, N. P., Ehsaan, M., Humphreys, C. M., Little, G. T., Baker, J., Henstra, A. M., …Zhang, Y. (2016). A roadmap for gene system development in Clostridium. Anaerobe, 41, 104-112. https://doi.org/10.1016/j.anaerobe.2016.05.011

Clostridium species are both heroes and villains. Some cause serious human and animal diseases, those present in the microbiota contribute to health and wellbeing, while others represent useful industrial chassis for the production of chemicals and f... Read More about A roadmap for gene system development in Clostridium.

Production of a functional cell wall-anchored minicellulosome by recombinant Clostridium acetobutylicum ATCC 824 (2016)
Journal Article
Willson, B. J., Kovács, K., Wilding-Steele, T., Markus, R., Winzer, K., & Minton, N. P. (2016). Production of a functional cell wall-anchored minicellulosome by recombinant Clostridium acetobutylicum ATCC 824. Biotechnology for Biofuels, 9(1), Article 109. https://doi.org/10.1186/s13068-016-0526-x

Background: The use of fossil fuels is no longer tenable. Not only are they a finite resource, their use is damaging the environment through pollution and global warming. Alternative, environmentally friendly, renewable sources of chemicals and fuels... Read More about Production of a functional cell wall-anchored minicellulosome by recombinant Clostridium acetobutylicum ATCC 824.

CRISPR-based genome editing and expression control systems in Clostridium acetobutylicum and Clostridium beijerinckii (2016)
Journal Article
Li, Q., Cheng, J., Minton, N. P., Zhang, Y., Wen, Z., Liu, J., …Yang, S. (2016). CRISPR-based genome editing and expression control systems in Clostridium acetobutylicum and Clostridium beijerinckii. Biotechnology Journal, 11(7), 961-972. https://doi.org/10.1002/biot.201600053

Solventogenic clostridia are important industrial microorganisms that produce various chemicals and fuels. Effective genetic tools would facilitate physiological studies aimed both at improving our understanding of metabolism and optimizing solvent p... Read More about CRISPR-based genome editing and expression control systems in Clostridium acetobutylicum and Clostridium beijerinckii.

Development of an inducible transposon system for efficient random mutagenesis in Clostridium acetobutylicum (2016)
Journal Article
Zhang, Y., Xu, S., Chai, C., Yang, S., Jiang, W., Minton, N. P., & Gu, Y. (2016). Development of an inducible transposon system for efficient random mutagenesis in Clostridium acetobutylicum. FEMS Microbiology Letters, 363(8), Article fnw065. https://doi.org/10.1093/femsle/fnw065

Clostridium acetobutylicum is an industrially important Gram-positive organism which is capable of producing economically important chemicals in the ABE (Acetone, Butanol and Ethanol) fermentation process. Renewed interests in the ABE process necessi... Read More about Development of an inducible transposon system for efficient random mutagenesis in Clostridium acetobutylicum.

Improving the reproducibility of the NAP1/B1/027 epidemic strain R20291 in the hamster model of infection (2016)
Journal Article
Kelly, M. L., Ng, Y. K., Cartman, S. T., Collery, M. M., Cockayne, A., & Minton, N. P. (2016). Improving the reproducibility of the NAP1/B1/027 epidemic strain R20291 in the hamster model of infection. Anaerobe, 39, 51-53. https://doi.org/10.1016/j.anaerobe.2016.02.011

Comparative analysis of the Clostridium difficile BI/NAP1/027 strain R20291 and ClosTron-derived ermB mutants in the hamster infection model are compromised by the clindamycin susceptibility of the parent. Mutants can appear more virulent. We have re... Read More about Improving the reproducibility of the NAP1/B1/027 epidemic strain R20291 in the hamster model of infection.

Mutant generation by allelic exchange and genome resequencing of the biobutanol organism Clostridium acetobutylicum ATCC 824 (2016)
Journal Article
Ehsaan, M., Kuit, W., Zhang, Y., Cartman, S. T., Heap, J. T., Winzer, K., & Minton, N. P. (2016). Mutant generation by allelic exchange and genome resequencing of the biobutanol organism Clostridium acetobutylicum ATCC 824. Biotechnology for Biofuels, 9(1), Article 4. https://doi.org/10.1186/s13068-015-0410-0

Background Clostridium acetobutylicum represents a paradigm chassis for the industrial production of the biofuel biobutanol and a focus for metabolic engineering. We have previously developed procedures for the creation of in-frame, marker-less de... Read More about Mutant generation by allelic exchange and genome resequencing of the biobutanol organism Clostridium acetobutylicum ATCC 824.