All Outputs (5)

Defining the intrinsically disordered C-terminal domain of SSB reveals DNA-mediated compaction (2015)
Journal Article
Green, M., Hatter, L., Brookes, E., Soultanas, P., & Scott, D. (2016). Defining the intrinsically disordered C-terminal domain of SSB reveals DNA-mediated compaction. Journal of Molecular Biology, 428(2), 357-364. https://doi.org/10.1016/j.jmb.2015.12.007

The bacterial single-stranded DNA (ssDNA) binding protein SSB is a strictly conserved and essential protein involved in diverse functions of DNA metabolism, including replication and repair. SSB comprises a well-characterized tetrameric core of N-ter... Read More about Defining the intrinsically disordered C-terminal domain of SSB reveals DNA-mediated compaction.

Comparison of the Structure and Activity of Glycosylated and Aglycosylated Human Carboxylesterase 1 (2015)
Journal Article
Arena de Souza, V., Scott, D. J., Nettleship, J. E., Rahman, N., Charlton, M. H., Walsh, M. A., & Owens, R. J. (2015). Comparison of the Structure and Activity of Glycosylated and Aglycosylated Human Carboxylesterase 1. PLoS ONE, 10(12), e0143919. https://doi.org/10.1371/journal.pone.0143919

Human Carboxylesterase 1 (hCES1) is the key liver microsomal enzyme responsible for detoxification and metabolism of a variety of clinical drugs. To analyse the role of the single N-linked glycan on the structure and activity of the enzyme, authentic... Read More about Comparison of the Structure and Activity of Glycosylated and Aglycosylated Human Carboxylesterase 1.

Evaluation of diffusion coefficients by means of an approximate steady-state condition in sedimentation velocity distributions (2015)
Journal Article
Scott, D. J., Harding, S. E., & Winzor, D. J. (2015). Evaluation of diffusion coefficients by means of an approximate steady-state condition in sedimentation velocity distributions. Analytical Biochemistry, 490, https://doi.org/10.1016/j.ab.2015.08.017

This investigation examined the feasibility of manipulating the rotor speed in sedimentation velocity experiments to spontaneously generate an approximate steady-state condition where the extent of diffusional spreading is matched exactly by the boun... Read More about Evaluation of diffusion coefficients by means of an approximate steady-state condition in sedimentation velocity distributions.

Crystal structures of the extracellular domain from PepT1 and PepT2 provide novel insights into mammalian pPeptide transport (2015)
Journal Article
Beale, J. H., Parker, J. L., Samsudin, F., Barrett, A. L., Senan, A., Bird, L. E., …Newstead, S. (2015). Crystal structures of the extracellular domain from PepT1 and PepT2 provide novel insights into mammalian pPeptide transport. Structure, 23(10), https://doi.org/10.1016/j.str.2015.07.016

Mammals obtain nitrogen via the uptake of di- and tri-peptides in the gastrointestinal tract through the action of PepT1 and PepT2, which are members of the POT family of proton-coupled oligopeptide transporters. PepT1 and PepT2 also play an importan... Read More about Crystal structures of the extracellular domain from PepT1 and PepT2 provide novel insights into mammalian pPeptide transport.

A Multilaboratory Comparison of Calibration Accuracy and the Performance of External References in Analytical Ultracentrifugation (2015)
Journal Article
Langowski, J., Zhao, H., Ghirlando, R., Alfonso, C., Arisaka, F., Attali, I., …Schuck, P. (2015). A Multilaboratory Comparison of Calibration Accuracy and the Performance of External References in Analytical Ultracentrifugation. PLoS ONE, 10(5), Article e0126420. https://doi.org/10.1371/journal.pone.0126420

Analytical ultracentrifugation (AUC) is a first principles based method to determine absolute sedimentation coefficients and buoyant molar masses of macromolecules and their complexes, reporting on their size and shape in free solution. The purpose o... Read More about A Multilaboratory Comparison of Calibration Accuracy and the Performance of External References in Analytical Ultracentrifugation.