Basement membrane influences intestinal epithelial cell growth and presents a barrier to the movement of macromolecules

Stolnik-Trenkic, Snow; Vllasaliua, Driton; Falcone, Franco H.; Garnett, Martin

doi:10.1016/j.yexcr.2014.02.022

All Outputs (4)

Improved expression and purification of the Helicobacter pylori adhesin BabA through the incorporation of a hexa-lysine tag (2014)
Journal Article
Hage, N., Falcone, F., Renshaw, J. G., Sebastiaan Winkler, G., Gellert, P., & Stolnik, S. (2015). Improved expression and purification of the Helicobacter pylori adhesin BabA through the incorporation of a hexa-lysine tag. Protein Expression and Purification, 106, 25-30. doi:10.1016/j.pep.2014.10.009

Helicobacter pylori is a pathogenic bacterium that has the remarkable ability to withstand the harsh conditions of the stomach for decades. This is achieved through unique evolutionary adaptations, which include binding Lewisb antigens found on the g... Read More about Improved expression and purification of the Helicobacter pylori adhesin BabA through the incorporation of a hexa-lysine tag.

The mechanisms of nanoparticle internalization and transport across an intestinal epithelial cell model: effect of size and surface charge (2014)
Journal Article
Bannunah, A. M., Vllasaliu, D., Lord, J., & Stolnik, . S. (2014). The mechanisms of nanoparticle internalization and transport across an intestinal epithelial cell model: effect of size and surface charge. Molecular Pharmaceutics, 11(12), 4363-4373. doi:10.1021/mp500439c

This study investigated the effect of nanoparticle size (50 and 100 nm) and surface charge on their interaction with Caco-2 monolayers as a model of the intestinal epithelium, including cell internalization pathways and the level of transepithelial t... Read More about The mechanisms of nanoparticle internalization and transport across an intestinal epithelial cell model: effect of size and surface charge.

Mechanism of mucosal permeability enhancement of CriticalSorb® (Solutol® HS15) investigated in vitro in cell cultures (2014)
Journal Article
Shubber, S., Vllasaliu, D., Rauch, C., Jordan, F., Illum, L., & Stolnik-Trenkic, S. (2015). Mechanism of mucosal permeability enhancement of CriticalSorb® (Solutol® HS15) investigated in vitro in cell cultures. Pharmaceutical Research, 32(2), 516-527. https://doi.org/10.1007/s11095-014-1481-5

Purpose: CriticalSorb™, with the principal component Solutol® HS15, is a novel mucosal drug delivery system demonstrated to improve the bioavailability of selected biotherapeutics. The intention of this study is to elucidate mechanism(s) responsible... Read More about Mechanism of mucosal permeability enhancement of CriticalSorb® (Solutol® HS15) investigated in vitro in cell cultures.

Basement membrane influences intestinal epithelial cell growth and presents a barrier to the movement of macromolecules (2014)
Journal Article
Stolnik-Trenkic, S., Vllasaliua, D., Falcone, F. H., & Garnett, M. (2014). Basement membrane influences intestinal epithelial cell growth and presents a barrier to the movement of macromolecules. Experimental Cell Research, 323(1), 218-231. https://doi.org/10.1016/j.yexcr.2014.02.022

This work examines the potential drug delivery barrier of the basement membrane (BM) by assessing the permeability of select macromolecules and nanoparticles. The study further extends to probing the effect of BM on intestinal epithelial cell attachm... Read More about Basement membrane influences intestinal epithelial cell growth and presents a barrier to the movement of macromolecules.