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All Outputs (59)

Validation of N-myristoyltransferase as an antimalarial drug target using an integrated chemical biology approach (2013)
Journal Article
Wright, M., Clough, B., Rackham, M., Rangachari, K., Brannigan, J., Grainger, M., …Tate, E. W. (2014). Validation of N-myristoyltransferase as an antimalarial drug target using an integrated chemical biology approach. Nature Chemistry, 6(2), 112-121. https://doi.org/10.1038/NCHEM.1830

Malaria is an infectious disease caused by parasites of the genus Plasmodium, which leads to approximately one million deaths per annum worldwide. Chemical validation of new antimalarial targets is urgently required in view of rising resistance to cu... Read More about Validation of N-myristoyltransferase as an antimalarial drug target using an integrated chemical biology approach.

Poly(Acrylic Acid)–Poly(Ethylene Glycol) Nanoparticles Designed for Ophthalmic Drug Delivery (2013)
Journal Article
Vasi, A., Popa, M. I., Tanase, E. C., Butnaru, M., & Verestiuc, L. (2014). Poly(Acrylic Acid)–Poly(Ethylene Glycol) Nanoparticles Designed for Ophthalmic Drug Delivery. Journal of Pharmaceutical Sciences, 103(2), 676-686. https://doi.org/10.1002/jps.23793

Poly(acrylic acid) (PAA) and poly(ethylene glycol) (PEG), four-arm, amine-terminated particles with nanometer size and spherical shape were obtained by the polymers cross-linking, via activation with 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide hy... Read More about Poly(Acrylic Acid)–Poly(Ethylene Glycol) Nanoparticles Designed for Ophthalmic Drug Delivery.

Kinetics and mechanism of G protein-coupled receptor activation (2013)
Journal Article
Lohse, M. J., Maiellaro, I., & Calebiro, D. (2014). Kinetics and mechanism of G protein-coupled receptor activation. Current Opinion in Cell Biology, 27, 87-93. https://doi.org/10.1016/j.ceb.2013.11.009

The activation of a G protein-coupled receptor is generally triggered by binding of an agonist to the receptor's binding pocket, or, in the case of rhodopsin, by light-induced changes of the pre-bound retinal. This is followed by a series of a confor... Read More about Kinetics and mechanism of G protein-coupled receptor activation.

Investigating the microrheology of the vitreous humor using an optically trapped local probe (2013)
Journal Article
Watts, F., Tan, L. E., Wilson, C. G., Girkin, J. M., Tassieri, M., & Wright, A. J. (2013). Investigating the microrheology of the vitreous humor using an optically trapped local probe. Journal of Optics, 16(1), https://doi.org/10.1088/2040-8978/16/1/015301

We demonstrate that an optically trapped silica bead can be used as a local probe to measure the micro-rheology of the vitreous humor. The Brownian motion of the bead was observed using a fast camera and the micro-rheology determined by analysis of t... Read More about Investigating the microrheology of the vitreous humor using an optically trapped local probe.

Investigation of the functional expression of purine and pyrimidine receptors in porcine isolated pancreatic arteries (2013)
Journal Article
Alsaqati, M., Chan, S., & Ralevic, V. (2014). Investigation of the functional expression of purine and pyrimidine receptors in porcine isolated pancreatic arteries. Purinergic Signalling, 10(2),

Receptors for purines and pyrimidines are expressed throughout the cardiovascular system. This study investigated their functional expression in porcine isolated pancreatic arteries. Pancreatic arteries (endothelium intact or denuded) were prepared f... Read More about Investigation of the functional expression of purine and pyrimidine receptors in porcine isolated pancreatic arteries.

Hyperactive mariner transposons are created by mutations that disrupt allosterism and increase the rate of transposon end synapsis (2013)
Journal Article
Liu, D., & Chalmers, R. (2014). Hyperactive mariner transposons are created by mutations that disrupt allosterism and increase the rate of transposon end synapsis. Nucleic Acids Research, 42(4), https://doi.org/10.1093/nar/gkt1218

New applications for transposons in vertebrate genetics have spurred efforts to develop hyperactive variants. Typically, a genetic screen is used to identify several hyperactive point mutations, which are then incorporated in a single transposase gen... Read More about Hyperactive mariner transposons are created by mutations that disrupt allosterism and increase the rate of transposon end synapsis.

Quantifying the range of a lipid phosphate signal in vivo (2013)
Journal Article
Mukherjee, A., Neher, R. A., & Renault, A. D. (2013). Quantifying the range of a lipid phosphate signal in vivo. Journal of Cell Science, 126(23), 5453-5464. https://doi.org/10.1242/jcs.136176

Quantitative information about the range of influence of extracellular signaling molecules is critical for understanding their effects, but is difficult to determine in the complex and dynamic three-dimensional environment of a living embryo. Drosoph... Read More about Quantifying the range of a lipid phosphate signal in vivo.

Neuron-glia crosstalk in health and disease: fractalkine and CX3CR1 take centre stage (2013)
Journal Article
Sheridan, G. K., & Murphy, K. J. (2013). Neuron-glia crosstalk in health and disease: fractalkine and CX3CR1 take centre stage. Open Biology, 3(12), Article 130181. https://doi.org/10.1098/rsob.130181

An essential aspect of normal brain function is the bidirectional interaction and communication between neurons and neighbouring glial cells. To this end, the brain has evolved ligand-receptor partnerships that facilitate crosstalk between different... Read More about Neuron-glia crosstalk in health and disease: fractalkine and CX3CR1 take centre stage.

Post-processing of polymer foam tissue scaffolds with high power ultrasound: A route to increased pore interconnectivity, pore size and fluid transport (2013)
Journal Article
Watson, N. J., Johal, R. K., Glover, Z., Reinwald, Y., White, L. J., Ghaemmaghami, A. M., …Parker, N. G. (2013). Post-processing of polymer foam tissue scaffolds with high power ultrasound: A route to increased pore interconnectivity, pore size and fluid transport. Materials Science and Engineering: C, 33(8), 4825-4832. https://doi.org/10.1016/j.msec.2013.07.046

The aimof this work is to demonstrate that the structural and fluidic properties of polymer foamtissue scaffolds, post-fabrication but prior to the introduction of cells, can be engineered via exposure to high power ultrasound. Our analysis is suppor... Read More about Post-processing of polymer foam tissue scaffolds with high power ultrasound: A route to increased pore interconnectivity, pore size and fluid transport.

Monoclonal anti-?1-adrenergic receptor antibodies activate G protein signaling in the absence of ?-arrestin recruitment (2013)
Journal Article
Hutchings, C. J., Cseke, G., Osborne, G., Woolard, J., Zhukov, A., Koglin, M., …Marshall, F. H. (2014). Monoclonal anti-β1-adrenergic receptor antibodies activate G protein signaling in the absence of β-arrestin recruitment. mAbs, 6(1), 246-261. https://doi.org/10.4161/mabs.27226

Thermostabilized G protein-coupled receptors used as antigens for in vivo immunization have resulted in the generation of functional agonistic anti-?1-adrenergic (?1AR) receptor monoclonal antibodies (mAbs). The focus of this study was to examine the... Read More about Monoclonal anti-?1-adrenergic receptor antibodies activate G protein signaling in the absence of ?-arrestin recruitment.

miR-24 affects hair follicle morphogenesis targeting Tcf-3 (2013)
Journal Article
Amelio, I., Lena, A. M., Bonanno, E., Melino, G., & Candi, E. (2013). miR-24 affects hair follicle morphogenesis targeting Tcf-3. Cell Death and Disease, 4(11), https://doi.org/10.1038/cddis.2013.426

During embryonic development, hair follicles (HFs) develop from an epidermal–mesenchymal cross talk between the ectoderm progenitor layer and the underlying dermis. Epidermal stem cell activation represents a crucial point both for HF morphogenesis a... Read More about miR-24 affects hair follicle morphogenesis targeting Tcf-3.

A structure-activity analysis of biased agonism at the dopamine D2 receptor. (2013)
Journal Article
Shonberg, J., Herenbrink, C. K., López, L., Christopoulos, A., Scammells, P. J., Capuano, B., & Lane, J. R. (2013). A structure-activity analysis of biased agonism at the dopamine D2 receptor. Journal of Medicinal Chemistry, 56(22), 9199-9221. https://doi.org/10.1021/jm401318w

Biased agonism offers an opportunity for the medicinal chemist to discover pathway-selective ligands for GPCRs. A number of studies have suggested that biased agonism at the dopamine D2 receptor (D2R) may be advantageous for the treatment of neuropsy... Read More about A structure-activity analysis of biased agonism at the dopamine D2 receptor..

Self-reporting Scaffolds for 3-Dimensional Cell Culture (2013)
Journal Article
Harrington, H., Rose, F. R., Aylott, J. W., & Ghaemmaghami, A. M. (2013). Self-reporting Scaffolds for 3-Dimensional Cell Culture. Journal of Visualized Experiments, https://doi.org/10.3791/50608

Culturing cells in 3D on appropriate scaffolds is thought to better mimic the in vivo microenvironment and increase cell-cell interactions. The resulting 3D cellular construct can often be more relevant to studying the molecular events and cell-cell... Read More about Self-reporting Scaffolds for 3-Dimensional Cell Culture.

Imaging Angiogenesis, Inflammation, and Metastasis in the Tumor Microenvironment with Magnetic Resonance Imaging (2013)
Book Chapter
O’Brien, E. R., Serres, S., & Sibson, N. R. (2013). Imaging Angiogenesis, Inflammation, and Metastasis in the Tumor Microenvironment with Magnetic Resonance Imaging. In Tumor Microenvironment and Cellular Stress (263-283). Springer New York. https://doi.org/10.1007/978-1-4614-5915-6_12

With the development of new imaging techniques, the potential for probing the molecular, cellular, and structural components of the tumor microenvironment in situ has increased dramatically. A multitude of imaging modalities have been successfully em... Read More about Imaging Angiogenesis, Inflammation, and Metastasis in the Tumor Microenvironment with Magnetic Resonance Imaging.

Increased function of pronociceptive TRPV1 at the level of the joint in a rat model of osteoarthritis pain (2013)
Journal Article
Kelly, S., Chapman, R., Woodhams, S., Sagar, D. R., Turner, J., Burston, J., …Chapman, V. (2014). Increased function of pronociceptive TRPV1 at the level of the joint in a rat model of osteoarthritis pain. Annals of the Rheumatic Diseases, 74, https://doi.org/10.1136/annrheumdis-2013-203413

Objectives Blockade of transient receptor potential vanilloid 1 (TRPV1) with systemic antagonists attenuates osteoarthritis (OA) pain behaviour in rat models, but on-target-mediated hyperthermia has halted clinical trials. The present study investiga... Read More about Increased function of pronociceptive TRPV1 at the level of the joint in a rat model of osteoarthritis pain.

Accuracy and efficiency define Bxb1 integrase as the best of fifteen candidate serine recombinases for the integration of DNA into the human genome (2013)
Journal Article
Xu, Z., Thomas, L., Davies, B., Chalmers, R., Smith, M., & Brown, W. (2013). Accuracy and efficiency define Bxb1 integrase as the best of fifteen candidate serine recombinases for the integration of DNA into the human genome. BMC Biotechnology, 13, Article 87. https://doi.org/10.1186/1472-6750-13-87

Background: Phage-encoded serine integrases, such as ?C31 integrase, are widely used for genome engineering. Fifteen such integrases have been described but their utility for genome engineering has not been compared in uniform assays. Results: We h... Read More about Accuracy and efficiency define Bxb1 integrase as the best of fifteen candidate serine recombinases for the integration of DNA into the human genome.

Accurate measurement of gene copy number for human alpha-defensin DEFA1A3 (2013)
Journal Article
Khan, F. F., Carpenter, D., Mitchell, L., Mansouri, O., Black, H. A., Tyson, J., & Armour, J. A. (2013). Accurate measurement of gene copy number for human alpha-defensin DEFA1A3. BMC Genomics, 14(719), https://doi.org/10.1186/1471-2164-14-719

Background: Multi-allelic copy number variants include examples of extensive variation between individuals in the copy number of important genes, most notably genes involved in immune function. The definition of this variation, and analysis of its i... Read More about Accurate measurement of gene copy number for human alpha-defensin DEFA1A3.