All Outputs (4)

Hyperactive mariner transposons are created by mutations that disrupt allosterism and increase the rate of transposon end synapsis (2013)
Journal Article
Liu, D., & Chalmers, R. (2014). Hyperactive mariner transposons are created by mutations that disrupt allosterism and increase the rate of transposon end synapsis. Nucleic Acids Research, 42(4), https://doi.org/10.1093/nar/gkt1218

New applications for transposons in vertebrate genetics have spurred efforts to develop hyperactive variants. Typically, a genetic screen is used to identify several hyperactive point mutations, which are then incorporated in a single transposase gen... Read More about Hyperactive mariner transposons are created by mutations that disrupt allosterism and increase the rate of transposon end synapsis.

Accuracy and efficiency define Bxb1 integrase as the best of fifteen candidate serine recombinases for the integration of DNA into the human genome (2013)
Journal Article
Xu, Z., Thomas, L., Davies, B., Chalmers, R., Smith, M., & Brown, W. (2013). Accuracy and efficiency define Bxb1 integrase as the best of fifteen candidate serine recombinases for the integration of DNA into the human genome. BMC Biotechnology, 13, Article 87. https://doi.org/10.1186/1472-6750-13-87

Background: Phage-encoded serine integrases, such as ?C31 integrase, are widely used for genome engineering. Fifteen such integrases have been described but their utility for genome engineering has not been compared in uniform assays. Results: We h... Read More about Accuracy and efficiency define Bxb1 integrase as the best of fifteen candidate serine recombinases for the integration of DNA into the human genome.

Identification of multiple binding sites for the THAP domain of the Galileo transposase in the long terminal inverted-repeats (2013)
Journal Article
Marzo, M., Liu, D., Ruiz, A., & Chalmers, R. (2013). Identification of multiple binding sites for the THAP domain of the Galileo transposase in the long terminal inverted-repeats. Gene, 525(1), https://doi.org/10.1016/j.gene.2013.04.050

Galileo is a DNA transposon responsible for the generation of several chromosomal inversions in Drosophila. In contrast to other members of the P-element superfamily, it has unusually long terminal inverted-repeats (TIRs) that resemble those of Foldb... Read More about Identification of multiple binding sites for the THAP domain of the Galileo transposase in the long terminal inverted-repeats.

Hsmar1 transposition is sensitive to the topology of the transposon donor and the target (2013)
Journal Article
Claeys Bouuaert, C., & Chalmers, R. (2013). Hsmar1 transposition is sensitive to the topology of the transposon donor and the target. PLoS ONE, 8(1), Article 0053690. https://doi.org/10.1371/journal.pone.0053690

Hsmar1 is a member of the Tc1-mariner superfamily of DNA transposons. These elements mobilize within the genome of their host by a cut-and-paste mechanism. We have exploited the in vitro reaction provided by Hsmar1 to investigate the effect of DNA su... Read More about Hsmar1 transposition is sensitive to the topology of the transposon donor and the target.