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Spores of Clostridium difficile clinical isolates display a diverse germination response to bile salts (2012)
Journal Article
Heeg, D., Burns, D. A., Cartman, S. T., & Minton, N. P. (2012). Spores of Clostridium difficile clinical isolates display a diverse germination response to bile salts. PLoS ONE, 7(2), Article e32381. https://doi.org/10.1371/journal.pone.0032381

Clostridium difficile spores play a pivotal role in the transmission of infectious diarrhoea, but in order to cause disease spores must complete germination and return to vegetative cell growth. While the mechanisms of spore germination are well unde... Read More about Spores of Clostridium difficile clinical isolates display a diverse germination response to bile salts.

Integration of DNA into bacterial chromosomes from plasmids without a counter-selection marker (2012)
Journal Article
Heap, J. T., Ehsaan, M., Cooksley, C. M., Ng, Y., Cartman, S. T., Winzer, K., & Minton, N. P. (2012). Integration of DNA into bacterial chromosomes from plasmids without a counter-selection marker. Nucleic Acids Research, 40(8), Article 10. https://doi.org/10.1093/nar/gkr1321

Most bacteria can only be transformed with circular plasmids, so robust DNA integration methods for these rely upon selection of single-crossover clones followed by counter-selection of double-crossover clones. To overcome the limited availability of... Read More about Integration of DNA into bacterial chromosomes from plasmids without a counter-selection marker.