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A novel synthesis and detection method for cap-associated adenosine modifications in mouse mRNA (2011)
Journal Article
Kruse, S., Zhong, S., Bodi, Z., Button, J., Alcocer, M. J., Hayes, C. J., & Fray, R. (2011). A novel synthesis and detection method for cap-associated adenosine modifications in mouse mRNA. Scientific Reports, 1, https://doi.org/10.1038/srep00126

A method is described for the detection of certain nucleotide modifications adjacent to the 5' 7-methyl guanosine cap of mRNAs from individual genes. The method quantitatively measures the relative abundance of 2'-O-methyl and N 6.

Native-state stability determines the extent of degradation relative to secretion of protein variants from Pichia pastoris (2011)
Journal Article
Whyteside, G., Alcocer, M. J., Kumita, J. R., Dobson, C. M., Lazarou, M., Pleass, R. J., & Archer, D. B. (2011). Native-state stability determines the extent of degradation relative to secretion of protein variants from Pichia pastoris. PLoS ONE, 6(7), Article e22692. https://doi.org/10.1371/journal.pone.0022692

We have investigated the relationship between the stability and secreted yield of a series of mutational variants of human lysozyme (HuL) in Pichia pastoris. We show that genes directly involved in the unfolded protein response (UPR), ER-associated d... Read More about Native-state stability determines the extent of degradation relative to secretion of protein variants from Pichia pastoris.