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Fructose-1,6-bisphosphate aldolase of Neisseria meningitidis binds human plasminogen via its C-terminal lysine residue (2016)
Journal Article
Shams, F., Oldfield, N. J., Lai, S. K., Tunio, S. A., Wooldridge, K. G., & Turner, D. P. (2016). Fructose-1,6-bisphosphate aldolase of Neisseria meningitidis binds human plasminogen via its C-terminal lysine residue. Microbiology Open, 5(2), https://doi.org/10.1002/mbo3.331

Neisseria meningitidis is a leading cause of fatal sepsis and meningitis worldwide. As for commensal species of human neisseriae, N. meningitidis inhabits the human nasopharynx and asymptomatic colonization is ubiquitous. Only rarely does the organis... Read More about Fructose-1,6-bisphosphate aldolase of Neisseria meningitidis binds human plasminogen via its C-terminal lysine residue.

Genomic analysis of serogroup Y Neisseria meningitidis isolates reveals extensive similarities between carriage and disease-associated organisms (2016)
Journal Article
Oldfield, N. J., Harrison, O. B., Bayliss, C. D., Maiden, M. C., Ala'Aldeen, D. A., & Turner, D. P. (2016). Genomic analysis of serogroup Y Neisseria meningitidis isolates reveals extensive similarities between carriage and disease-associated organisms. Journal of Infectious Diseases, 213(11), 1777-1785. https://doi.org/10.1093/infdis/jiw008

Background. Neisseria meningitidis is a frequent colonizer of the human nasopharynx with asymptomatic carriage providing the reservoir for invasive, disease-causing strains. Serogroup Y (MenY) strains are a major cause of meningococcal disease. High... Read More about Genomic analysis of serogroup Y Neisseria meningitidis isolates reveals extensive similarities between carriage and disease-associated organisms.

Optimization of molecular approaches to genogroup Neisseria meningitidis carriage isolates and implications for monitoring the impact of new serogroup B vaccines (2015)
Journal Article
Rojas, E., Hoyos, J., Oldfield, N., Lee, P., Flint, M., Jones, C. H., …Anderson, A. S. (2015). Optimization of molecular approaches to genogroup Neisseria meningitidis carriage isolates and implications for monitoring the impact of new serogroup B vaccines. PLoS ONE, 10, Article e0132140. https://doi.org/10.1371/journal.pone.0132140

Nuclear trafficking, histone cleavage and induction of apoptosis by the meningococcal App and MspA autotransporters (2015)
Journal Article
Khairalla, A. S., Omer, S. A., Mahdavi, J., Aslam, A., Dufailu, O. A., Self, T., …Ala'Aldeen, D. A. (2015). Nuclear trafficking, histone cleavage and induction of apoptosis by the meningococcal App and MspA autotransporters. Cellular Microbiology, 17(7), 1008-1020. https://doi.org/10.1111/cmi.12417

Neisseria meningitidis, a major cause of bacterial meningitis and septicaemia, secretes multiple virulence factors, including the adhesion and penetration protein (App) and meningococcal serine protease A (MspA). Both are conserved, immunogenic, type... Read More about Nuclear trafficking, histone cleavage and induction of apoptosis by the meningococcal App and MspA autotransporters.

Fructose-1,6-bisphosphate aldolase (FBA)-A conserved glycolytic enzyme with virulence functions in bacteria: 'Ill met by moonlight' (2014)
Journal Article
Shams, F., Oldfield, N. J., Wooldridge, K. G., & Turner, D. P. J. (2014). Fructose-1,6-bisphosphate aldolase (FBA)-A conserved glycolytic enzyme with virulence functions in bacteria: 'Ill met by moonlight'. Biochemical Society Transactions, 42(6), 1792-1795. https://doi.org/10.1042/BST20140203

© 2014 Biochemical Society. Moonlighting proteins constitute an intriguing class of multifunctional proteins. Metabolic enzymes and chaperones, which are often highly conserved proteins in bacteria, archaea and eukaryotic organisms, are among the mos... Read More about Fructose-1,6-bisphosphate aldolase (FBA)-A conserved glycolytic enzyme with virulence functions in bacteria: 'Ill met by moonlight'.

Deciphering the complex three-way interaction between the non-integrin laminin receptor, galectin-3 and Neisseria meningitidis (2014)
Journal Article
Alqahtani, F. Y. S., Mahdavi, J., Wheldon, L. M., Vassey, M., Pirinccioglu, N., Royer, P., …Ala'Aldeen, D. A. (2014). Deciphering the complex three-way interaction between the non-integrin laminin receptor, galectin-3 and Neisseria meningitidis. Open Biology, 4(10), Article 140053. https://doi.org/10.1098/rsob.140053

The non-integrin laminin receptor (LAMR1/RPSA) and galectin-3 (Gal-3) are multi-functional host molecules with roles in diverse pathological processes, particularly of infectious or oncogenic origins. Using bimolecular fluorescence complementation an... Read More about Deciphering the complex three-way interaction between the non-integrin laminin receptor, galectin-3 and Neisseria meningitidis.

A novel O-linked glycan modulates Campylobacter jejuni major outer membrane protein-mediated adhesion to human histo-blood group antigens and chicken colonization (2014)
Journal Article
Mahdavi, J., Pirinccioglu, N., Oldfield, N. J., Carlsohn, E., Stoof, J., Aslam, A., …Ala'Aldeen, D. A. (2014). A novel O-linked glycan modulates Campylobacter jejuni major outer membrane protein-mediated adhesion to human histo-blood group antigens and chicken colonization. Open Biology, 4(1), Article 130202. https://doi.org/10.1098/rsob.130202

Campylobacter jejuni is an important cause of human foodborne gastroenteritis; strategies to prevent infection are hampered by a poor understanding of the complex interactions between host and pathogen. Previous work showed that C. jejuni could bind... Read More about A novel O-linked glycan modulates Campylobacter jejuni major outer membrane protein-mediated adhesion to human histo-blood group antigens and chicken colonization.

Prevalence and phase variable expression status of two autotransporters, NalP and MspA, in carriage and disease isolates of Neisseria meningitidis (2013)
Journal Article
Oldfield, N. J., Matar, S., Bidmos, F. A., Alamro, M., Neal, K. R., Turner, D. P., …Ala'Aldeen, D. A. (2013). Prevalence and phase variable expression status of two autotransporters, NalP and MspA, in carriage and disease isolates of Neisseria meningitidis. PLoS ONE, 8(7), Article e69746. https://doi.org/10.1371/journal.pone.0069746

Neisseria meningitidis is a human nasopharyngeal commensal capable of causing life-threatening septicemia and meningitis. Many meningococcal surface structures, including the autotransporter proteins NalP and MspA, are subject to phase variation (PV)... Read More about Prevalence and phase variable expression status of two autotransporters, NalP and MspA, in carriage and disease isolates of Neisseria meningitidis.

Mapping the laminin receptor binding domains of Neisseria meningitidis PorA and Haemophilus influenzae OmpP2 (2012)
Journal Article
Abouseada, N. M., Assafi, M. S. A., Mahdavi, J., Oldfield, N. J., Wheldon, L. M., Wooldridge, K. G., & Ala'Aldeen, D. A. (2012). Mapping the laminin receptor binding domains of Neisseria meningitidis PorA and Haemophilus influenzae OmpP2. PLoS ONE, 7(9), Article 10. https://doi.org/10.1371/journal.pone.0046233

Neisseria meningitidis, Haemophilus influenzae and Streptococcus pneumoniae are major bacterial agents of meningitis. They each bind the 37/67-kDa laminin receptor (LamR) via the surface protein adhesins: meningococcal PilQ and PorA, H. influenzae Om... Read More about Mapping the laminin receptor binding domains of Neisseria meningitidis PorA and Haemophilus influenzae OmpP2.

Cj1136 is required for lipooligosaccharide biosynthesis, hyperinvasion, and chick colonization by Campylobacter jejuni (2012)
Journal Article
Javed, M. A., Cawthraw, S. A., Baig, A., Li, J., McNally, A., Oldfield, N. J., …Manning, G. (2012). Cj1136 is required for lipooligosaccharide biosynthesis, hyperinvasion, and chick colonization by Campylobacter jejuni. Infection and Immunity, 80(7), 2361-2370. https://doi.org/10.1128/IAI.00151-12

Campylobacter jejuni is a major cause of bacterial food-borne enteritis worldwide, and invasion into intestinal epithelial cells is an important virulence mechanism. Recently we reported the identification of hyperinvasive C. jejuni strains and creat... Read More about Cj1136 is required for lipooligosaccharide biosynthesis, hyperinvasion, and chick colonization by Campylobacter jejuni.

Persistence, replacement, and rapid clonal expansion of meningococcal carriage isolates in a 2008 university student cohort (2011)
Journal Article
Bidmos, F. A., Neal, K. R., Oldfield, N. J., Turner, D. P., Ala'Aldeen, D. A., & Bayliss, C. D. (2011). Persistence, replacement, and rapid clonal expansion of meningococcal carriage isolates in a 2008 university student cohort. Journal of Clinical Microbiology, 49(2), 506-512. https://doi.org/10.1128/JCM.01322-10

A study of meningococcal carriage dynamics was performed with a cohort of 190 first-year students recruited from six residential halls at Nottingham University, United Kingdom. Pharyngeal swabs were obtained on four occasions between November 2008 an... Read More about Persistence, replacement, and rapid clonal expansion of meningococcal carriage isolates in a 2008 university student cohort.

The role of glyceraldehyde 3-phosphate dehydrogenase (GapA-1) in Neisseria meningitidis adherence to human cells (2010)
Journal Article
Tunio, S. A., Oldfield, N. J., Ala'Aldeen, D. A. A., Wooldridge, K. G., & Turner, D. P. J. (2010). The role of glyceraldehyde 3-phosphate dehydrogenase (GapA-1) in Neisseria meningitidis adherence to human cells. BMC Microbiology, 10, Article 280. https://doi.org/10.1186/1471-2180-10-280

Background Glyceraldehyde 3-phosphate dehydrogenases (GAPDHs) are cytoplasmic glycolytic enzymes, which although lacking identifiable secretion signals, have also been found localized to the surface of several bacteria (and some eukaryotic organisms... Read More about The role of glyceraldehyde 3-phosphate dehydrogenase (GapA-1) in Neisseria meningitidis adherence to human cells.

Human antibody responses to the meningococcal factor H binding protein (LP2086) during invasive disease, colonization and carriage (2010)
Journal Article
Ala'Aldeen, D. A., Flint, M., Oldfield, N. J., Omer, S. A., McNeil, L. K., Jiang, Q., …Anderson, A. S. (2010). Human antibody responses to the meningococcal factor H binding protein (LP2086) during invasive disease, colonization and carriage. Vaccine, 28(48), 7667-7675. https://doi.org/10.1016/j.vaccine.2010.09.038

Recombinant forms of Neisseria meningitidis factor H binding protein (fHBP) are undergoing clinical trials in candidate vaccines against serogroup B meningococcal disease. Little is known, however, about the host response to fHBP during natural carri... Read More about Human antibody responses to the meningococcal factor H binding protein (LP2086) during invasive disease, colonization and carriage.

The moonlighting protein fructose-1, 6-bisphosphate aldolase of Neisseria meningitidis: Surface localization and role in host cell adhesion (2010)
Journal Article
Tunio, S. A., Oldfield, N. J., Berry, A., Ala'Aldeen, D. A. A., Wooldridge, K. G., & Turner, D. P. J. (2010). The moonlighting protein fructose-1, 6-bisphosphate aldolase of Neisseria meningitidis: Surface localization and role in host cell adhesion. Molecular Microbiology, 76(3), 605-615. https://doi.org/10.1111/j.1365-2958.2010.07098.x

Fructose-1, 6-bisphosphate aldolases (FBA) are cytoplasmic glycolytic enzymes, which despite lacking identifiable secretion signals, have also been found localized to the surface of several bacteria where they bind host molecules and exhibit non-glyc... Read More about The moonlighting protein fructose-1, 6-bisphosphate aldolase of Neisseria meningitidis: Surface localization and role in host cell adhesion.

AasP autotransporter protein of Actinobacillus pleuropneumoniae does not protect pigs against homologous challenge (2009)
Journal Article
Oldfield, N. J., Worrall, K. E., Rycroft, A. N., Ali, T., Wooldridge, K. G., & Ala'Aldeen, D. A. (2009). AasP autotransporter protein of Actinobacillus pleuropneumoniae does not protect pigs against homologous challenge. Vaccine, 27(38), 5278-5283. https://doi.org/10.1016/j.vaccine.2009.06.047

Actinobacillus pleuropneumoniae is a major respiratory pathogen of pigs; current vaccines provide only limited protection. AasP, a subtilisin-like serine protease, is a conserved outer membrane-localised autotransporter protein. We hypothesized that... Read More about AasP autotransporter protein of Actinobacillus pleuropneumoniae does not protect pigs against homologous challenge.

Laminin receptor initiates bacterial contact with the blood brain barrier in experimental meningitis models (2009)
Journal Article
Orihuela, C. J., Mahdavi, J., Thornton, J., Mann, B., Wooldridge, K. G., Abouseada, N., …Tuomanen, E. I. (2009). Laminin receptor initiates bacterial contact with the blood brain barrier in experimental meningitis models. Journal of Clinical Investigation, 119(6), 1638-1646. https://doi.org/10.1172/JCI36759

A diverse array of infectious agents, including prions and certain neurotropic viruses, bind to the laminin receptor (LR), and this determines tropism to the CNS. Bacterial meningitis in childhood is almost exclusively caused by the respiratory tract... Read More about Laminin receptor initiates bacterial contact with the blood brain barrier in experimental meningitis models.

Functional Characterization of AasP, a Maturation Protease Autotransporter Protein of Actinobacillus pleuropneumoniae (2008)
Journal Article
Ali, T., Oldfield, N. J., Wooldridge, K. G., Turner, D. P., & Ala'Aldeen, D. A. A. (2008). Functional Characterization of AasP, a Maturation Protease Autotransporter Protein of Actinobacillus pleuropneumoniae. Infection and Immunity, 76(12), 5608-5614. https://doi.org/10.1128/IAI.00085-08

Actinobacillus pleuropneumoniae is the etiological agent of porcine pleuropneumonia, a highly contagious respiratory infection in pigs. AasP, a putative subtilisin-like serine protease autotransporter, has recently been identified in A. pleuropneumon... Read More about Functional Characterization of AasP, a Maturation Protease Autotransporter Protein of Actinobacillus pleuropneumoniae.

Identification and characterization of novel antigenic vaccine candidates of Actinobacillus pleuropneumoniae (2008)
Journal Article
Oldfield, N. J., Donovan, E. A., Worrall, K. E., Wooldridge, K. G., Langford, P. R., Rycroft, A. N., & Ala'Aldeen, D. A. (2008). Identification and characterization of novel antigenic vaccine candidates of Actinobacillus pleuropneumoniae. Vaccine, 26(16), 1942-1954. https://doi.org/10.1016/j.vaccine.2008.02.022

Actinobacillus pleuropneumoniae is an important respiratory pathogen of swine, for which there is no highly effective vaccine. A phage expression library of the A. pleuropneumoniae genome was constructed and screened to identify potential vaccine com... Read More about Identification and characterization of novel antigenic vaccine candidates of Actinobacillus pleuropneumoniae.

CapA, an Autotransporter Protein of Campylobacter jejuni, Mediates Association with Human Epithelial Cells and Colonization of the Chicken Gut (2007)
Journal Article
Ashgar, S. S., Oldfield, N. J., Wooldridge, K. G., Jones, M. A., Irving, G. J., Turner, D. P., & Ala'Aldeen, D. A. (2007). CapA, an Autotransporter Protein of Campylobacter jejuni, Mediates Association with Human Epithelial Cells and Colonization of the Chicken Gut. Journal of Bacteriology, 189(5), 1856-1865. https://doi.org/10.1128/JB.01427-06

Two putative autotransporter proteins, CapA and CapB, were identified in silico from the genome sequence of Campylobacter jejuni NCTC11168. The genes encoding each protein contain homopolymeric tracts, suggestive of phase variation mediated by a slip... Read More about CapA, an Autotransporter Protein of Campylobacter jejuni, Mediates Association with Human Epithelial Cells and Colonization of the Chicken Gut.