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Ex vivo assessment of basal cell carcinoma surgical margins in Mohs surgery by autofluorescence‐Raman spectroscopy: A pilot study (2023)
Journal Article
Boitor, R., Varma, S., Sharma, A., Elsheikh, S., Kulkarni, K., Eldib, K., …Notingher, I. (2023). Ex vivo assessment of basal cell carcinoma surgical margins in Mohs surgery by autofluorescence‐Raman spectroscopy: A pilot study. JEADV Clinical Practice, https://doi.org/10.1002/jvc2.336

Background: Autofluorescence (AF)‐Raman spectroscopy is a technology that can detect tumour tissue in surgically excised skin specimens. The technique does not require tissue fixation, staining, labelling or sectioning, and provides quantitative diag... Read More about Ex vivo assessment of basal cell carcinoma surgical margins in Mohs surgery by autofluorescence‐Raman spectroscopy: A pilot study.

Optimization of diffuse Raman spectroscopy for in-vivo quantification of foreign body response in a small animal model (2023)
Journal Article
Dooley, M., Luckett, J., Alexander, M. R., Matousek, P., Dehghani, H., Ghaemmaghami, A. M., & Notingher, I. (2023). Optimization of diffuse Raman spectroscopy for in-vivo quantification of foreign body response in a small animal model. Biomedical Optics Express, 14(12), 6592-6606. https://doi.org/10.1364/boe.512118

Diffuse Raman spectroscopy (DRS) allows subsurface molecular analysis of optically turbid samples. Numerical modeling of light propagation was used as a method for improving the design of an DRS instrument to maximize the signal to noise ratio (SNR)... Read More about Optimization of diffuse Raman spectroscopy for in-vivo quantification of foreign body response in a small animal model.

Optimisation of diffuse Raman spectroscopy for in-vivo quantification of foreign body response in small animal model (2023)
Journal Article
Dooley, M., Luckett, J., Alexander, M. R., Matousek, P., Dehghani, H., Ghaemmaghami, A. M., & Notingher, I. (2023). Optimisation of diffuse Raman spectroscopy for in-vivo quantification of foreign body response in small animal model. Biomedical Optics Express, 14(12), 6592-6606. https://doi.org/10.1364/BOE.512118

Diffuse Raman spectroscopy (DRS) allows subsurface molecular analysis of optically turbid samples. Numerical modelling of light propagation was used as a method for improving the design of an DRS instrument to maximize the signal to noise ratio (SNR)... Read More about Optimisation of diffuse Raman spectroscopy for in-vivo quantification of foreign body response in small animal model.

Real-time ex-vivo assessment of basal cell carcinoma surgical margins in Mohs surgery by autofluorescence-Raman spectroscopy: a pilot study (2023)
Journal Article
Boitor, R., Varma, V., Sharma, A., Elsheikh, S., Kulkarni, K., Eldib, K., …Notingher, I. (in press). Real-time ex-vivo assessment of basal cell carcinoma surgical margins in Mohs surgery by autofluorescence-Raman spectroscopy: a pilot study. Journal of the European Academy of Dermatology and Venereology,

Background Autofluorescence (AF)-Raman spectroscopy is a technology that can detect tumour tissue in surgically excised skin specimens. The technique does not require tissue fixation, staining, labelling or sectioning, and provides quantitative diag... Read More about Real-time ex-vivo assessment of basal cell carcinoma surgical margins in Mohs surgery by autofluorescence-Raman spectroscopy: a pilot study.

Autofluorescence-Raman Spectroscopy for Ex Vivo Mapping Colorectal Liver Metastases and Liver Tissue (2023)
Journal Article
Corden, C., Boitor, R., Dusanjh, P. K., Harwood, A., Mukherjee, A., Gomez, D., & Notingher, I. (2023). Autofluorescence-Raman Spectroscopy for Ex Vivo Mapping Colorectal Liver Metastases and Liver Tissue. Journal of Surgical Research, 288, 10-20. https://doi.org/10.1016/j.jss.2023.02.014

Introduction: Identifying colorectal liver metastases (CRLM) during liver resection could assist in achieving clear surgical margins, which is an important prognostic variable for both disease-free and overall survival. The aim of this study was to i... Read More about Autofluorescence-Raman Spectroscopy for Ex Vivo Mapping Colorectal Liver Metastases and Liver Tissue.

Quantification of Drugs in Brain and Liver Mimetic Tissue Models Using Raman Spectroscopy (2022)
Journal Article
Woodhouse, N., Majer, J., Marshall, P., Hood, S., & Notingher, I. (2022). Quantification of Drugs in Brain and Liver Mimetic Tissue Models Using Raman Spectroscopy. Applied Spectroscopy, https://doi.org/10.1177/00037028221139494

Quantitative analysis of drug delivery with in biological systems is an integral challenge in drug development. Analytical techniques are important for assessing both drug target delivery, target action, and drug toxicology. Using mimetic tissue mode... Read More about Quantification of Drugs in Brain and Liver Mimetic Tissue Models Using Raman Spectroscopy.

Model-Based Optimization of Laser Excitation and Detection Improves Spectral Contrast in Noninvasive Diffuse Raman Spectroscopy (2022)
Journal Article
Dooley, M. J., Paterson, T., Dexter, L., Matousek, P., Dehghani, H., & Notingher, I. (2022). Model-Based Optimization of Laser Excitation and Detection Improves Spectral Contrast in Noninvasive Diffuse Raman Spectroscopy. Applied Spectroscopy, 76(7), 801-811. https://doi.org/10.1177/00037028211072900

Spatially offset Raman spectroscopy (SORS) is a powerful technique for subsurface molecular analysis of optically turbid samples. Numerical modeling of light propagation has been used to investigate opportunities for improving spectral contrast and s... Read More about Model-Based Optimization of Laser Excitation and Detection Improves Spectral Contrast in Noninvasive Diffuse Raman Spectroscopy.

Intra-operative assessment of sentinel lymph nodes for breast cancer surgery: An update (2021)
Journal Article
Barkur, S., Notingher, I., & Rakha, E. (2022). Intra-operative assessment of sentinel lymph nodes for breast cancer surgery: An update. Surgical Oncology, 40, Article 101678. https://doi.org/10.1016/j.suronc.2021.101678

Lymph node (LN) involvement is the strongest prognostic factor in operable breast cancer (BC). Therefore, accurate assessment of LN status is essential for management of BC patients. The introduction of sentinel LN approach reduced the need for exten... Read More about Intra-operative assessment of sentinel lymph nodes for breast cancer surgery: An update.

Time-gated Raman spectroscopy for biomedical application under ambient or strong background light conditions (2021)
Journal Article
Corden, C., Boitor, R., & Notingher, I. (2021). Time-gated Raman spectroscopy for biomedical application under ambient or strong background light conditions. Journal of Physics D: Applied Physics, 54(50), Article 504003. https://doi.org/10.1088/1361-6463/ac276e

Many biomedical applications require measurements of Raman spectra of tissue under ambient lighting conditions. However, the background light often swamps the weaker Raman signal. The use of time-gated (TG) Raman spectroscopy based on a single photon... Read More about Time-gated Raman spectroscopy for biomedical application under ambient or strong background light conditions.

Selective-sampling Raman imaging techniques for ex vivo assessment of surgical margins in cancer surgery (2021)
Journal Article
Lizio, M. G., Boitor, R., & Notingher, I. (2021). Selective-sampling Raman imaging techniques for ex vivo assessment of surgical margins in cancer surgery. Analyst, 146(12), 3799-3809. https://doi.org/10.1039/d1an00296a

One of the main challenges in cancer surgery is to ensure the complete excision of the tumour while sparing as much healthy tissue as possible. Histopathology, the gold-standard technique used to assess the surgical margins on the excised tissue, is... Read More about Selective-sampling Raman imaging techniques for ex vivo assessment of surgical margins in cancer surgery.

Clinical integration of fast Raman spectroscopy for Mohs micrographic surgery of basal cell carcinoma (2021)
Journal Article
Boitor, R., de Wolf, C., Weesie, F., Shipp, D. W., Varma, S., Veitch, D., …Notingher, I. (2021). Clinical integration of fast Raman spectroscopy for Mohs micrographic surgery of basal cell carcinoma. Biomedical Optics Express, 12(4), 2015-2026. https://doi.org/10.1364/boe.417896

We present the first clinical integration of a prototype device based on integrated auto-fluorescence imaging and Raman spectroscopy (Fast Raman device) for intra-operative assessment of surgical margins during Mohs micrographic surgery of basal cell... Read More about Clinical integration of fast Raman spectroscopy for Mohs micrographic surgery of basal cell carcinoma.

Combined total internal reflection AF spectral-imaging and Raman spectroscopy for fast assessment of surgical margins during breast cancer surgery (2021)
Journal Article
Lizio, M. G., Liao, Z., Shipp, D. W., Boitor, R., Mihai, R., Sharp, J. S., …Notingher, I. (2021). Combined total internal reflection AF spectral-imaging and Raman spectroscopy for fast assessment of surgical margins during breast cancer surgery. Biomedical Optics Express, 12(2), 940-954. https://doi.org/10.1364/boe.411648

The standard treatment for breast cancer is surgical removal mainly through breast conserving surgey (BCS). We developed a new technique based on auto-fluorescence (AF) spectral imaging and Raman spectroscopy for fast intraoperative assessment of exc... Read More about Combined total internal reflection AF spectral-imaging and Raman spectroscopy for fast assessment of surgical margins during breast cancer surgery.

Sub-Surface Molecular Analysis and Imaging in Turbid Media Using Time-Gated Raman Spectral Multiplexing (2020)
Journal Article
Corden, C., Matousek, P., Conti, C., & Notingher, I. (2021). Sub-Surface Molecular Analysis and Imaging in Turbid Media Using Time-Gated Raman Spectral Multiplexing. Applied Spectroscopy, 75(2), 156-167. https://doi.org/10.1177/0003702820946054

Obtaining molecular information deeper within optically turbid samples is valuable in many applications. However, in many cases this is challenging, in particular when the sample elicits strong laser-induced fluorescence emission. Here, we investigat... Read More about Sub-Surface Molecular Analysis and Imaging in Turbid Media Using Time-Gated Raman Spectral Multiplexing.

Feasibility of integrated high?wavenumber Raman imaging and fingerprint Raman spectroscopy for fast margin assessment in breast cancer surgery (2020)
Journal Article
Liao, Z., Lizio, M. G., Corden, C., Khout, H., Rakha, E., & Notingher, I. (2020). Feasibility of integrated high?wavenumber Raman imaging and fingerprint Raman spectroscopy for fast margin assessment in breast cancer surgery. Journal of Raman Spectroscopy, 51(10), 1986-1995. https://doi.org/10.1002/jrs.5937

Intraoperative assessment of surgical margins remains one of the main challenges in cancer surgery. Raman spectroscopy can detect cancer cells with high accuracy, but it is time‐consuming. In this paper, we investigated a selective‐sampling Raman spe... Read More about Feasibility of integrated high?wavenumber Raman imaging and fingerprint Raman spectroscopy for fast margin assessment in breast cancer surgery.

Induction and measurement of the early stage of a host-parasite interaction using a combined optical trapping and Raman microspectroscopy system (2019)
Journal Article
Sinjab, F., Elsheikha, H. M., Dooley, M., & Notingher, I. (2020). Induction and measurement of the early stage of a host-parasite interaction using a combined optical trapping and Raman microspectroscopy system. Journal of Biophotonics, 13(2), https://doi.org/10.1002/jbio.201960065

© 2019 The Authors. Journal of Biophotonics published by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim Understanding and quantifying the temporal acquisition of host cell molecules by intracellular pathogens is fundamentally important in biology. In t... Read More about Induction and measurement of the early stage of a host-parasite interaction using a combined optical trapping and Raman microspectroscopy system.

Raman spectroscopy study of curvature-mediated lipid packing and sorting in single lipid vesicles (2019)
Journal Article
Collard, L., Sinjab, F., & Notingher, I. (2019). Raman spectroscopy study of curvature-mediated lipid packing and sorting in single lipid vesicles. Biophysical Journal, 117(9), 1589-1598. https://doi.org/10.1016/j.bpj.2019.09.020

Cellular plasma membrane deformability and stability is important in a range of biological processes. Changes in local curvature of the membrane affects the lateral movement of lipids, affecting the biophysical properties of the membrane. An integrat... Read More about Raman spectroscopy study of curvature-mediated lipid packing and sorting in single lipid vesicles.

Ex-vivo Raman spectroscopy mapping of lung tissue: label-free molecular characterisation of non-tumorous and cancerous tissues (2019)
Journal Article
Bourbousson, M., Soomro, I., Baldwin, D., & Notingher, I. (2019). Ex-vivo Raman spectroscopy mapping of lung tissue: label-free molecular characterisation of non-tumorous and cancerous tissues. Journal of Medical Imaging, 6(3), Article 036001. https://doi.org/10.1117/1.JMI.6.3.036001

Raman spectroscopy mapping was used to study ex vivo fresh lung tissues and compare to histology sections. The Raman mapping measurements revealed differences in the molecular composition of normal lung tissue, adenocarcinoma, and squamous cell carci... Read More about Ex-vivo Raman spectroscopy mapping of lung tissue: label-free molecular characterisation of non-tumorous and cancerous tissues.

Applications of Spatial Light Modulators in Raman Spectroscopy (2019)
Journal Article
Sinjab, F., Liao, Z., & Notingher, I. (2019). Applications of Spatial Light Modulators in Raman Spectroscopy. Applied Spectroscopy, 73(7), 727-746. https://doi.org/10.1177/0003702819834575

Advances in consumer display screen technologies have historically been adapted by researchers across the fields of optics as they can be used as electronically controlled spatial light modulators (SLMs) for a variety of uses. The performance charact... Read More about Applications of Spatial Light Modulators in Raman Spectroscopy.

Spatially-offset Raman spectroscopy for monitoring mineralization of bone tissue engineering scaffolds: feasibility study based on phantom samples (2019)
Journal Article
Dooley, M., Prasopthum, A., Liao, Z., Sinjab, F., Mclaren, J., Rose, F. R. A. J., …Notingher, I. (2019). Spatially-offset Raman spectroscopy for monitoring mineralization of bone tissue engineering scaffolds: feasibility study based on phantom samples. Biomedical Optics Express, 10(4), 1678-1690. https://doi.org/10.1364/BOE.10.001678

Using phantom samples, we investigated the feasibility of spatially-offset Raman spectroscopy (SORS) as a tool for monitoring non-invasively the mineralization of bone tissue engineering scaffold in-vivo. The phantom samples consisted of 3D-printed s... Read More about Spatially-offset Raman spectroscopy for monitoring mineralization of bone tissue engineering scaffolds: feasibility study based on phantom samples.