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Recombinant ovine prion protein can be mutated at position 136 to improve its efficacy as an inhibitor of prion propagation (2023)
Journal Article
Kopycka, K., Maddison, B. C., & Gough, K. C. (2023). Recombinant ovine prion protein can be mutated at position 136 to improve its efficacy as an inhibitor of prion propagation. Scientific Reports, 13(1), Article 3452. https://doi.org/10.1038/s41598-023-30202-0

Prion diseases are progressive neurodegenerative disorders with no effective therapeutics. The central event leading to the pathology in the diseases is the conversion of PrPC into PrPSc and its accumulation in the central nervous system. Previous st... Read More about Recombinant ovine prion protein can be mutated at position 136 to improve its efficacy as an inhibitor of prion propagation.

Therapeutic Phage Display-Derived Single-Domain Antibodies for Pandemic Preparedness (2023)
Journal Article
Daly, J. M., Lim, T. S., & Gough, K. C. (2023). Therapeutic Phage Display-Derived Single-Domain Antibodies for Pandemic Preparedness. Antibodies, 12(1), Article 7. https://doi.org/10.3390/antib12010007

Driven by necessity, the COVID-19 pandemic caused by SARS-CoV-2 has accelerated the development and implementation of new vaccine platforms and other viral therapeutics. Among these is the therapeutic use of antibodies including single-domain antibod... Read More about Therapeutic Phage Display-Derived Single-Domain Antibodies for Pandemic Preparedness.

A Simple Whole-Plasmid PCR Method to Construct High-Diversity Synthetic Phage Display Libraries (2022)
Journal Article
Tsoumpeli, M. T., Gray, A., Parsons, A. L., Spiliotopoulos, A., Owen, J. P., Bishop, K., …Gough, K. C. (2022). A Simple Whole-Plasmid PCR Method to Construct High-Diversity Synthetic Phage Display Libraries. Molecular Biotechnology, 64, 791-803. https://doi.org/10.1007/s12033-021-00442-4

Phage display technology utilises peptide and antibody libraries with very high diversities to select ligands with specific binding properties. The production of such libraries can be labour intensive and technically challenging and whilst there are... Read More about A Simple Whole-Plasmid PCR Method to Construct High-Diversity Synthetic Phage Display Libraries.

BSE can propagate in sheep co-infected or pre-infected with scrapie (2021)
Journal Article
Chong, A., Foster, J. D., Goldmann, W., Gonzalez, L., Jeffrey, M., O'Connor, M. J., …Hunter, N. (2021). BSE can propagate in sheep co-infected or pre-infected with scrapie. Scientific Reports, 11, Article 11931. https://doi.org/10.1038/s41598-021-91397-8

To understand the possible role of mixed-prion infections in disease presentation, the current study reports the co-infection of sheep with bovine spongiform encephalopathy (BSE) and scrapie. The bovine BSE agent was inoculated subcutaneously into sh... Read More about BSE can propagate in sheep co-infected or pre-infected with scrapie.

The ‘dark matter’ of ubiquitin-mediated processes: opportunities and challenges in the identification of ubiquitin-binding domains (2019)
Journal Article
Radley, E., Long, J., Gough, K., & Layfield, R. (2019). The ‘dark matter’ of ubiquitin-mediated processes: opportunities and challenges in the identification of ubiquitin-binding domains. Biochemical Society Transactions, 47(6), 1949-1962. https://doi.org/10.1042/bst20190869

Ubiquitin modifications of target proteins act to localise, direct and specify a diverse range of cellular processes, many of which are biomedically relevant. To allow this diversity, ubiquitin modifications exhibit remarkable complexity, determined... Read More about The ‘dark matter’ of ubiquitin-mediated processes: opportunities and challenges in the identification of ubiquitin-binding domains.

Advances in the Production and Batch Reformatting of Phage Antibody Libraries (2019)
Journal Article
Reader, R. H., Workman, R. G., Maddison, B. C., & Gough, K. C. (2019). Advances in the Production and Batch Reformatting of Phage Antibody Libraries. Molecular Biotechnology, 61, 801-815. https://doi.org/10.1007/s12033-019-00207-0

Phage display antibody libraries have proven an invaluable resource for the isolation of diagnostic and potentially therapeutic antibodies, the latter usually being antibody fragments converted into IgG formats. Recent advances in the production of h... Read More about Advances in the Production and Batch Reformatting of Phage Antibody Libraries.

BSE infectivity survives burial for five years with only limited spread (2019)
Journal Article
Somerville, R. A., Fernie, K., Smith, A., Bishop, K., Maddison, B. C., Gough, K. C., …Gough, K. C. (2019). BSE infectivity survives burial for five years with only limited spread. Archives of Virology, 164(4), 1135–1145. https://doi.org/10.1007/s00705-019-04154-8

© 2019, The Author(s). The carcasses of animals infected with bovine spongiform encephalopathy (BSE), scrapie or chronic wasting disease (CWD) that remain in the environment (exposed or buried) may continue to act as reservoirs of infectivity. We co... Read More about BSE infectivity survives burial for five years with only limited spread.

Discovery of peptide ligands targeting a specific ubiquitin-like domain– binding site in the deubiquitinase USP11 (2018)
Journal Article
Spiliotopoulos, A., Ferreras, L. B., Densham, R. M., Caulton, S. G., Maddison, B. C., Morris, J. R., …Dreveny, I. (2019). Discovery of peptide ligands targeting a specific ubiquitin-like domain– binding site in the deubiquitinase USP11. Journal of Biological Chemistry, 294(2), 424-436. https://doi.org/10.1074/jbc.RA118.004469

© 2019 Spiliotopoulos et al. Published by The American Society for Biochemistry and Molecular Biology, Inc. Ubiquitin-specific proteases (USPs) reverse ubiquitination and regulate virtually all cellular processes. Defined noncatalytic domains in USP... Read More about Discovery of peptide ligands targeting a specific ubiquitin-like domain– binding site in the deubiquitinase USP11.

Needle in a haystack? A comparison of eDNA metabarcoding and targeted qPCR for detection of the great crested newt (Triturus cristatus) (2018)
Journal Article
Harper, L. R., Lawson Handley, L., Hahn, C., Boonham, N., Rees, H. C., Gough, K. C., …Hänfling, B. (2018). Needle in a haystack? A comparison of eDNA metabarcoding and targeted qPCR for detection of the great crested newt (Triturus cristatus). Ecology and Evolution, 8(12), 6330-6341. https://doi.org/10.1002/ece3.4013

Environmental DNA (eDNA) analysis is a rapid, cost-effective, non-invasive biodiversity monitoring tool which utilises DNA left behind in the environment by organisms for species detection. The method is used as a species-specific survey tool for rar... Read More about Needle in a haystack? A comparison of eDNA metabarcoding and targeted qPCR for detection of the great crested newt (Triturus cristatus).

The chronic stages of bovine Fasciola hepatica are dominated by CD4 T-Cell exhaustion (2017)
Journal Article
Sachdev, D., Gough, K. C., & Flynn, R. J. (2017). The chronic stages of bovine Fasciola hepatica are dominated by CD4 T-Cell exhaustion. Frontiers in Immunology, 8, Article 1002. https://doi.org/10.3389/fimmu.2017.01002

Fasciola hepatica infection of ruminants leads to non-resolving chronic infection, as patency develops, there is switching to a TGF-? and IL-10 led response. Here, we explore the responses of CD4 T-cells within the major draining lymph nodes. We foun... Read More about The chronic stages of bovine Fasciola hepatica are dominated by CD4 T-Cell exhaustion.

The detection of great crested newts year round via environmental DNA analysis (2017)
Journal Article
Rees, H. C., Baker, C. A., Gardner, D. S., Maddison, B. C., & Gough, K. C. (2017). The detection of great crested newts year round via environmental DNA analysis. BMC Research Notes, 10(1), Article 327. https://doi.org/10.1186/s13104-017-2657-y

Objective Analysis of environmental DNA (eDNA) is a method that has been used for the detection of various species within water bodies. The great crested newt (Triturus cristatus) has a short eDNA survey season (mid-April to June). Here we investi... Read More about The detection of great crested newts year round via environmental DNA analysis.

Ovine recombinant PrP as an inhibitor of ruminant prion propagation in vitro (2017)
Journal Article
Maddison, B. C., Workman, R. G., & Gough, K. C. (in press). Ovine recombinant PrP as an inhibitor of ruminant prion propagation in vitro. Prion, 11(4), https://doi.org/10.1080/19336896.2017.1342919

Prion diseases are fatal and incurable neurodegenerative diseases of humans and animals. Despite years of research, no therapeutic agents have been developed that can effectively manage or reverse disease progression. Recently it has been identified... Read More about Ovine recombinant PrP as an inhibitor of ruminant prion propagation in vitro.

An in vitro model for assessing effective scrapie decontamination (2017)
Journal Article
Gough, K. C., Baker, C., & Maddison, B. (2017). An in vitro model for assessing effective scrapie decontamination. Veterinary Microbiology, 207, https://doi.org/10.1016/j.vetmic.2017.05.018

Scrapie infectivity enters the environment via a multiplicity of routes from infected animals. Environmentally associated scrapie persists on farms when infected animals have been removed and is particularly resistant to disinfection. Infectivity wit... Read More about An in vitro model for assessing effective scrapie decontamination.

How is Europe positioned for a re-emergence of Schmallenberg virus? (2017)
Journal Article
Tarlinton, R., Gough, K., Maddison, B., Stavrou, A., Daly, J. M., Maddison, B. C., …Tarlinton, R. E. (2017). How is Europe positioned for a re-emergence of Schmallenberg virus?. Veterinary Journal, 230, 45-51. https://doi.org/10.1016/j.tvjl.2017.04.009

The Schmallenberg virus (SBV) caused a large scale epidemic in Europe from 2011–2013 infecting ruminants and causing fetal deformities after infection of pregnant animals. The main impacts of the virus were financial losses due to animal, meat and se... Read More about How is Europe positioned for a re-emergence of Schmallenberg virus?.

In vitro amplification of H-type atypical bovine spongiform encephalopathy by protein misfolding cyclic amplification (2017)
Journal Article
O'Connor, M., Bishop, K., Workman, R. G., Maddison, B. C., & Gough, K. C. (in press). In vitro amplification of H-type atypical bovine spongiform encephalopathy by protein misfolding cyclic amplification. Prion, 11(1), 54-64. https://doi.org/10.1080/19336896.2016.1259051

The in vitro amplification of prions by serial protein misfolding cyclic amplification has been shown to detect PrPSc to levels at least as sensitive as rodent bioassay but in a fraction of the time. Bovine spongiform encephalopathy is a zoonotic pri... Read More about In vitro amplification of H-type atypical bovine spongiform encephalopathy by protein misfolding cyclic amplification.

A Trematode Parasite Derived Growth Factor Binds and Exerts Influences on Host Immune Functions via Host Cytokine Receptor Complexes (2016)
Journal Article
Sulaiman, A. A., Zolnierczyk, K., Japa, O., Owen, J. P., Maddison, B. C., Emes, R. D., …Flynn, R. J. (2016). A Trematode Parasite Derived Growth Factor Binds and Exerts Influences on Host Immune Functions via Host Cytokine Receptor Complexes. PLoS Pathogens, 12(11), Article 1005991. https://doi.org/10.1371/journal.ppat.1005991

© 2016 Sulaiman et al. The trematode Fasciola hepatica is responsible for chronic zoonotic infection globally. Despite causing a potent T-helper 2 response, it is believed that potent immunomodulation is responsible for rendering this host reactive n... Read More about A Trematode Parasite Derived Growth Factor Binds and Exerts Influences on Host Immune Functions via Host Cytokine Receptor Complexes.

A sensitive 301V BSE serial PMCA assay (2016)
Journal Article
Gough, K. C., Bishop, K., Somerville, R. A., Hunter, N., & Maddison, B. C. (2016). A sensitive 301V BSE serial PMCA assay. F1000Research, 5(2529), https://doi.org/10.12688/f1000research.9735.1

The prion strain 301V, is a mouse passaged form of bovine spongiform encephalopathy (BSE). It has been used as a model of BSE for more than 20 years, in particular in the investigation of tissue distribution of infectivity, the molecular phenotype an... Read More about A sensitive 301V BSE serial PMCA assay.

Mapping B-cell responses to Salmonella enterica serovars Typhimurium and Enteritidis in chickens for the discrimination of infected from vaccinated animals (2016)
Journal Article
Naqid, I. A., Owen, J. P., Maddison, B. C., Spiliotopoulos, A., Emes, R. D., Warry, A., …Gough, K. C. (2016). Mapping B-cell responses to Salmonella enterica serovars Typhimurium and Enteritidis in chickens for the discrimination of infected from vaccinated animals. Scientific Reports, 6, Article 31186. https://doi.org/10.1038/srep31186

Serological surveillance and vaccination are important strategies for controlling infectious diseases of food production animals. However, the compatibility of these strategies is limited by a lack of assays capable of di erentiating infected from va... Read More about Mapping B-cell responses to Salmonella enterica serovars Typhimurium and Enteritidis in chickens for the discrimination of infected from vaccinated animals.

Mapping polyclonal antibody responses to bacterial infection using next generation phage display (2016)
Journal Article
Naqid, I. A., Owen, J. P., Maddison, B. C., Spiliotopoulos, A., Emes, R. D., Warry, A., …Gough, K. C. (2016). Mapping polyclonal antibody responses to bacterial infection using next generation phage display. Scientific Reports, 6, Article 24232. https://doi.org/10.1038/srep24232

Mapping polyclonal antibody responses to infectious diseases to identify individual epitopes has the potential to underpin the development of novel serological assays and vaccines. Here, phage-peptide library panning coupled with screening using next... Read More about Mapping polyclonal antibody responses to bacterial infection using next generation phage display.

Methods for differentiating prion types in food-producing animals (2015)
Journal Article
Gough, K. C., Rees, H. C., Ives, S. E., & Maddison, B. C. (2015). Methods for differentiating prion types in food-producing animals. Biology, 4(4), https://doi.org/10.3390/biology4040785

Prions are an enigma amongst infectious disease agents as they lack a genome yet confer specific pathologies thought to be dictated mainly, if not solely, by the conformation of the disease form of the prion protein (PrPSc). Prion diseases affect hum... Read More about Methods for differentiating prion types in food-producing animals.