All Outputs (11)

Label-free Brillouin endo-microscopy for the quantitative 3D imaging of sub-micrometre biology (2024)
Journal Article
La Cavera III, S., Chauhan, V. M., Hardiman, W., Yao, M., Fuentes-Domínguez, R., Setchfield, K., …Clark, M. (2024). Label-free Brillouin endo-microscopy for the quantitative 3D imaging of sub-micrometre biology. Communications Biology, 7, Article 451. https://doi.org/10.1038/s42003-024-06126-4

This report presents an optical fibre-based endo-microscopic imaging tool that simultaneously measures the topographic profile and 3D viscoelastic properties of biological specimens through the phenomenon of time-resolved Brillouin scattering. This u... Read More about Label-free Brillouin endo-microscopy for the quantitative 3D imaging of sub-micrometre biology.

Effect of skin color on optical properties and the implications for medical optical technologies: a review (2024)
Journal Article
Setchfield, K., Gorman, A., Simpson, A. H. R. W., Somekh, M. G., & Wright, A. J. (2024). Effect of skin color on optical properties and the implications for medical optical technologies: a review. Journal of Biomedical Optics, 29(01), Article 010901. https://doi.org/10.1117/1.jbo.29.1.010901

Significance: Skin color affects light penetration leading to differences in its absorption and scattering properties. COVID-19 highlighted the importance of understanding of the interaction of light with different skin types, e.g., pulse oximetry (P... Read More about Effect of skin color on optical properties and the implications for medical optical technologies: a review.

Development of hydrogel-based standards and phantoms for non-linear imaging at depth (2023)
Journal Article
Haseeb, F., Bourdakos, K. N., Forsyth, E., Setchfield, K., Gorman, A., Venkateswaran, S., …Bradley, M. (2023). Development of hydrogel-based standards and phantoms for non-linear imaging at depth. Journal of Biomedical Optics, 28(12), Article 126007. https://doi.org/10.1117/1.JBO.28.12.126007%5D

Significance: Rapid advances in medical imaging technology, particularly the development of optical systems with non-linear imaging modalities, are boosting deep tissue imaging. The development of reliable standards and phantoms is critical for valid... Read More about Development of hydrogel-based standards and phantoms for non-linear imaging at depth.

Relevance and utility of the in-vivo and ex-vivo optical properties of the skin reported in the literature: a review [Invited] (2023)
Journal Article
Setchfield, K., Gorman, A., Simpson, A. H. R. W., Somekh, M. G., & Wright, A. J. (2023). Relevance and utility of the in-vivo and ex-vivo optical properties of the skin reported in the literature: a review [Invited]. Biomedical Optics Express, 14(7), 3555-3583. https://doi.org/10.1364/boe.493588

Imaging non-invasively into the human body is currently limited by cost (MRI and CT scan), image resolution (ultrasound), exposure to ionising radiation (CT scan and X-ray), and the requirement for exogenous contrast agents (CT scan and PET scan). Op... Read More about Relevance and utility of the in-vivo and ex-vivo optical properties of the skin reported in the literature: a review [Invited].

Living cells as a biological analog of optical tweezers – a non-invasive microrheology approach (2023)
Journal Article
Hardiman, W., Clark, M., Friel, C., Huett, A., Pérez-Cota, F., Setchfield, K., …Tassieri, M. (2023). Living cells as a biological analog of optical tweezers – a non-invasive microrheology approach. Acta Biomaterialia, 166, 317-325. https://doi.org/10.1016/j.actbio.2023.04.039

Microrheology, the study of fluids on micron length-scales, promises to reveal insights into cellular biology, including mechanical biomarkers of disease and the interplay between biomechanics and cellular function. Here a minimally-invasive passive... Read More about Living cells as a biological analog of optical tweezers – a non-invasive microrheology approach.

Parallel imaging with phonon microscopy using a multi-core fibre bundle detection (2023)
Journal Article
Fuentes-Domínguez, R., Yao, M., Hardiman, W., La Cavera III, S., Setchfield, K., Pérez-Cota, F., …Clark, M. (2023). Parallel imaging with phonon microscopy using a multi-core fibre bundle detection. Photoacoustics, 31, Article 100493. https://doi.org/10.1016/j.pacs.2023.100493

In this paper, we show a proof-of-concept method to parallelise phonon microscopy measurements for cell elasticity imaging by demonstrating a 3-fold increase in acquisition speed which is limited by current acquisition hardware. Phonon microscopy is... Read More about Parallel imaging with phonon microscopy using a multi-core fibre bundle detection.

A U-Shape Fibre-Optic pH Sensor Based on Hydrogen Bonding of Ethyl Cellulose with a Sol-Gel Matrix (2020)
Journal Article
Tang, Z., Gomez, D., He, C., Korposh, S., Morgan, S. P., Correia, R., …Liu, L. (2021). A U-Shape Fibre-Optic pH Sensor Based on Hydrogen Bonding of Ethyl Cellulose with a Sol-Gel Matrix. Journal of Lightwave Technology, 39(5), 1557-1564. https://doi.org/10.1109/JLT.2020.3034563

© 1983-2012 IEEE. Development of a biocompatible pH sensor is of importance in biomedical applications, particularly for in vivo measurement, providing necessary information for clinical diagnosis and treatment such as chronic wounds and foetal acido... Read More about A U-Shape Fibre-Optic pH Sensor Based on Hydrogen Bonding of Ethyl Cellulose with a Sol-Gel Matrix.

Picosecond ultrasonics for elasticity-based imaging and characterization of biological cells (2020)
Journal Article
Pérez-Cota, F., Fuentes-Domínguez, R., La Cavera III, S., Hardiman, W., Yao, M., Setchfield, K., …Clark, M. (2020). Picosecond ultrasonics for elasticity-based imaging and characterization of biological cells. Journal of Applied Physics, 128(16), Article 160902. https://doi.org/10.1063/5.0023744

© 2020 Author(s). Characterization of the elasticity of biological cells is growing as a new way to gain insight into cell biology. Cell mechanics are related to most aspects of cellular behavior, and applications in research and medicine are broad.... Read More about Picosecond ultrasonics for elasticity-based imaging and characterization of biological cells.

Tropomyosin 1: multiple roles in the developing heart and in the formation of congenital heart defects (2017)
Journal Article
England, J., Granados-Riveron, J. T., Polo-Parada, L., Kuriakose, D., Moore, C., Brook, J. D., …Loughna, S. (2017). Tropomyosin 1: multiple roles in the developing heart and in the formation of congenital heart defects. Journal of Molecular and Cellular Cardiology, 106, https://doi.org/10.1016/j.yjmcc.2017.03.006

Tropomyosin 1 (TPM1) is an essential sarcomeric component, stabilising the thin filament and facilitating actin's interaction with myosin. A number of sarcomeric proteins, such as alpha myosin heavy chain, play crucial roles in cardiac development. M... Read More about Tropomyosin 1: multiple roles in the developing heart and in the formation of congenital heart defects.

Distinct genetic architectures for syndromic and nonsyndromic congenital heart defects identified by exome sequencing (2016)
Journal Article
Sifrim, A., Hitz, M., Wilsdon, A., Breckpot, J., Turki, S. H. A., Thienpont, B., …Hurles, M. E. (2016). Distinct genetic architectures for syndromic and nonsyndromic congenital heart defects identified by exome sequencing. Nature Genetics, 48(9), 1060-1065. https://doi.org/10.1038/ng.3627

Congenital heart defects (CHDs) have a neonatal incidence of 0.8–1%. Despite abundant examples of monogenic CHD in humans and mice, CHD has a low absolute sibling recurrence risk (~2.7%), suggesting a considerable role for de novo mutations (DNMs) an... Read More about Distinct genetic architectures for syndromic and nonsyndromic congenital heart defects identified by exome sequencing.

Combined mutation screening of NKX2-5, GATA4, and TBX5 in congenital heart disease: multiple heterozygosity and novel mutations (2012)
Journal Article
Granados-Riveron, J. T., Pope, M., Bu'Lock, F. A., Thornborough, C., Eason, J., Setchfield, K., …Brook, D. (2012). Combined mutation screening of NKX2-5, GATA4, and TBX5 in congenital heart disease: multiple heterozygosity and novel mutations. Congenital Heart Disease, 7(2), https://doi.org/10.1111/j.1747-0803.2011.00573.x

Background: Variants of several genes encoding transcription modulators, signal transduction, and structural proteins are known to cause Mendelian congenital heart disease (CHD). NKX2-5 and GATA4 were the first CHD-causing genes identified by linkage... Read More about Combined mutation screening of NKX2-5, GATA4, and TBX5 in congenital heart disease: multiple heterozygosity and novel mutations.