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Perlecan (HSPG2) promotes structural, contractile, and metabolic development of human cardiomyocytes (2024)
Journal Article
Johnson, B. B., Cosson, M., Tsansizi, L. I., Holmes, T. L., Gilmore, T., Hampton, K., …Smith, J. G. W. (2024). Perlecan (HSPG2) promotes structural, contractile, and metabolic development of human cardiomyocytes. Cell Reports, 43(1), Article 113668. https://doi.org/10.1016/j.celrep.2023.113668

Perlecan (HSPG2), a heparan sulfate proteoglycan similar to agrin, is key for extracellular matrix (ECM) maturation and stabilization. Although crucial for cardiac development, its role remains elusive. We show that perlecan expression increases as c... Read More about Perlecan (HSPG2) promotes structural, contractile, and metabolic development of human cardiomyocytes.

Human model of primary carnitine deficiency cardiomyopathy reveals ferroptosis as a novel mechanism (2023)
Journal Article
Loos, M., Klampe, B., Schulze, T., Yin, X., Theofilatos, K., Ulmer, B. M., …Hansen, A. (2023). Human model of primary carnitine deficiency cardiomyopathy reveals ferroptosis as a novel mechanism. Stem Cell Reports, 18(11), 2123-2137. https://doi.org/10.1016/j.stemcr.2023.09.002

Primary carnitine deficiency (PCD) is an autosomal recessive monogenic disorder caused by mutations in SLC22A5. This gene encodes for OCTN2, which transports the essential metabolite carnitine into the cell. PCD patients suffer from muscular weakness... Read More about Human model of primary carnitine deficiency cardiomyopathy reveals ferroptosis as a novel mechanism.

Process evaluation of a university residence-based SARS-CoV-2 testing programme in the UK (2022)
Journal Article
Blake, H., Carlisle, S., Fothergill, L., Hassard, J., Favier, A., Corner, J., …Denning, C. (2022). Process evaluation of a university residence-based SARS-CoV-2 testing programme in the UK. European Journal of Public Health, 32(Supplement_3), Article ckac131.046. https://doi.org/10.1093/eurpub/ckac131.046

Background Regular testing for SARS-CoV-2 is an important strategy for controlling virus outbreaks on university campuses during the COVID-19 pandemic but testing participation can be low. The Residence-Based Testing Participation Pilot (RB-TPP) was... Read More about Process evaluation of a university residence-based SARS-CoV-2 testing programme in the UK.

A Qualitative Evaluation of the Barriers and Enablers for Implementation of an Asymptomatic SARS-CoV-2 Testing Service at the University of Nottingham: A Multi-Site Higher Education Setting in England (2022)
Journal Article
Blake, H., Somerset, S., Mahmood, I., Mahmood, N., Corner, J., Ball, J. K., & Denning, C. (2022). A Qualitative Evaluation of the Barriers and Enablers for Implementation of an Asymptomatic SARS-CoV-2 Testing Service at the University of Nottingham: A Multi-Site Higher Education Setting in England. International Journal of Environmental Research and Public Health, 19(20), Article 13140. https://doi.org/10.3390/ijerph192013140

Asymptomatic testing for SARS-CoV-2 RNA has been used to prevent and manage COVID-19 outbreaks in university settings, but few studies have explored their implementation. The aim of the study was to evaluate how an accredited asymptomatic SARS-CoV-2... Read More about A Qualitative Evaluation of the Barriers and Enablers for Implementation of an Asymptomatic SARS-CoV-2 Testing Service at the University of Nottingham: A Multi-Site Higher Education Setting in England.

Workforce Experiences of a Rapidly Established SARS-CoV-2 Asymptomatic Testing Service in a Higher Education Setting: A Qualitative Study (2022)
Journal Article
Blake, H., Somerset, S., Mahmood, I., Mahmood, N., Corner, J., Ball, J. K., & Denning, C. (2022). Workforce Experiences of a Rapidly Established SARS-CoV-2 Asymptomatic Testing Service in a Higher Education Setting: A Qualitative Study. International Journal of Environmental Research and Public Health, 19(19), Article 12464. https://doi.org/10.3390/ijerph191912464

The aim of the study was to explore workforce experiences of the rapid implementation of a SARS-CoV-2 asymptomatic testing service (ATS) in a higher education setting during the COVID-19 pandemic. The setting was a multi-campus university in the UK,... Read More about Workforce Experiences of a Rapidly Established SARS-CoV-2 Asymptomatic Testing Service in a Higher Education Setting: A Qualitative Study.

Mixed-methods process evaluation of a residence-based SARS-CoV-2 testing participation pilot on a UK university campus during the COVID-19 pandemic (2022)
Journal Article
Blake, H., Carlisle, S., Fothergill, L., Hassard, J., Favier, A., Corner, J., …Denning, C. (2022). Mixed-methods process evaluation of a residence-based SARS-CoV-2 testing participation pilot on a UK university campus during the COVID-19 pandemic. BMC Public Health, 22(1), Article 1470. https://doi.org/10.1186/s12889-022-13792-8

Background: Regular testing for Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) is an important strategy for controlling virus outbreaks on university campuses during the COVID-19 pandemic but testing participation rates can be low. The... Read More about Mixed-methods process evaluation of a residence-based SARS-CoV-2 testing participation pilot on a UK university campus during the COVID-19 pandemic.

Performance evaluation of a non-invasive one-step multiplex RT-qPCR assay for detection of SARS-CoV-2 direct from saliva (2022)
Journal Article
Jenkins, H. H., Lopez, A. A. T., Tarantini, F. S., Tomlin, H., Scales, D., Lee, I., …Seedhouse, C. (2022). Performance evaluation of a non-invasive one-step multiplex RT-qPCR assay for detection of SARS-CoV-2 direct from saliva. Scientific Reports, 12(1), Article 11553. https://doi.org/10.1038/s41598-022-15616-6

Polymerase chain reaction (PCR) has proven to be the gold-standard for SARS-CoV-2 detection in clinical settings. The most common approaches rely on nasopharyngeal specimens obtained from swabs, followed by RNA extraction, reverse transcription and q... Read More about Performance evaluation of a non-invasive one-step multiplex RT-qPCR assay for detection of SARS-CoV-2 direct from saliva.

Exploring the Psychological Impacts of COVID-19 Social Restrictions on International University Students: A Qualitative Study (2022)
Journal Article
Al-Oraibi, A., Fothergill, L., Yildirim, M., Knight, H., Carlisle, S., O’Connor, M., …Blake, H. (2022). Exploring the Psychological Impacts of COVID-19 Social Restrictions on International University Students: A Qualitative Study. International Journal of Environmental Research and Public Health, 19(13), Article 7631. https://doi.org/10.3390/ijerph19137631

The global COVID-19 pandemic has impacted on the mental well-being of university students, but little attention has been given to international students, who may have a unique experience and perspective. The aim of this study was to explore the views... Read More about Exploring the Psychological Impacts of COVID-19 Social Restrictions on International University Students: A Qualitative Study.

The changing vaccine landscape: rates of COVID-19 vaccine acceptance and hesitancy in young adults during vaccine rollout (2022)
Journal Article
Knight, H., Jia, R., Ayling, K., Blake, H., Morling, J. R., Villalon, A. M., …Vedhara, K. (2023). The changing vaccine landscape: rates of COVID-19 vaccine acceptance and hesitancy in young adults during vaccine rollout. Perspectives in Public Health, 143(4), 220-224. https://doi.org/10.1177/17579139221094750

Aims: Development and rollout of vaccines offers the best opportunity for population protection against the SARS-CoV-2 (COVID-19) virus. However, hesitancy towards the vaccines might impede successful uptake in the United Kingdom, particularly in you... Read More about The changing vaccine landscape: rates of COVID-19 vaccine acceptance and hesitancy in young adults during vaccine rollout.

Direct RT-qPCR Assay for the Detection of SARS-CoV-2 in Saliva Samples (2022)
Journal Article
Tarantini, F. S., Wu, S., Jenkins, H., Lopez, A. T., Tomlin, H., Hyde, R., …Benest, A. V. (2022). Direct RT-qPCR Assay for the Detection of SARS-CoV-2 in Saliva Samples. Methods and Protocols, 5(2), Article 25. https://doi.org/10.3390/mps5020025

Since mid-2020 there have been complexities and difficulties in the standardisation and administration of nasopharyngeal swabs. Coupled with the variable and/or poor accuracy of lateral flow devices, this has led to increased societal ‘testing fatigu... Read More about Direct RT-qPCR Assay for the Detection of SARS-CoV-2 in Saliva Samples.

Quantifiable correlation of ToF-SIMS and XPS data from polymer surfaces with controlled amino acid and peptide content (2022)
Journal Article
Taylor, M., Simoes, F., Smith, J., Genapathy, S., Canning, A., Lledos, M., …Zelzer, M. (2022). Quantifiable correlation of ToF-SIMS and XPS data from polymer surfaces with controlled amino acid and peptide content. Surface and Interface Analysis, 54(4), 417-432. https://doi.org/10.1002/sia.7052

Peptide-coated surfaces are widely employed in biomaterial design, but quantifiable correlation between surface composition and biological response is challenging due to, for example, instrumental limitations, a lack of suitable model surfaces or lim... Read More about Quantifiable correlation of ToF-SIMS and XPS data from polymer surfaces with controlled amino acid and peptide content.

Relationship between Anxiety, Depression, and Susceptibility to Severe Acute Respiratory Syndrome Coronavirus 2 Infection: Proof of Concept (2022)
Journal Article
Vedhara, K., Ayling, K., Jia, R., Fairclough, L., Morling, J. R., Ball, J. K., …Tighe, P. (2022). Relationship between Anxiety, Depression, and Susceptibility to Severe Acute Respiratory Syndrome Coronavirus 2 Infection: Proof of Concept. Journal of Infectious Diseases, 225(12), 2137-2141. https://doi.org/10.1093/infdis/jiac006

Background: Psychological factors can influence susceptibility to viral infections. We examined whether such influences are evident in severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. Methods: Participants (n=102) completed mea... Read More about Relationship between Anxiety, Depression, and Susceptibility to Severe Acute Respiratory Syndrome Coronavirus 2 Infection: Proof of Concept.

Saliva for COVID-19 testing: Simple but useless or an undervalued resource? (2021)
Journal Article
Pijuan-Galito, S., Tarantini, F. S., Tomlin, H., Jenkins, H., Thompson, J. L., Scales, D., …Denning, C. (2021). Saliva for COVID-19 testing: Simple but useless or an undervalued resource?. Frontiers in Virology, 1, Article 778790. https://doi.org/10.3389/fviro.2021.778790

During the COVID-19 pandemic, countries with robust population-based asymptomatic testing were generally successful in controlling virus spread, hence reducing hospitalizations and deaths. This effectiveness inspired widespread asymptomatic surveilla... Read More about Saliva for COVID-19 testing: Simple but useless or an undervalued resource?.

Transcriptomic Analysis of Cardiomyocyte Extracellular Vesicles in Hypertrophic Cardiomyopathy Reveals Differential snoRNA Cargo (2021)
Journal Article
James, V., Nizamudeen, Z. A., Lea, D., Dottorini, T., Holmes, T. L., Johnson, B. B., …Smith, J. G. (2021). Transcriptomic Analysis of Cardiomyocyte Extracellular Vesicles in Hypertrophic Cardiomyopathy Reveals Differential snoRNA Cargo. Stem Cells and Development, 30(24), 1215-1227. https://doi.org/10.1089/scd.2021.0202

Hypertrophic cardiomyopathy (HCM) is characterised by increased left ventricular wall thickness that can lead to devastating conditions such as heart failure and sudden cardiac death. Despite extensive study, the mechanisms mediating many of the asso... Read More about Transcriptomic Analysis of Cardiomyocyte Extracellular Vesicles in Hypertrophic Cardiomyopathy Reveals Differential snoRNA Cargo.

Impacts of the COVID-19 pandemic and self-isolation on students and staff in higher education: A qualitative study (2021)
Journal Article
Knight, H., Carlisle, S., O’connor, M., Briggs, L., Fothergill, L., Al-Oraibi, A., …Blake, H. (2021). Impacts of the COVID-19 pandemic and self-isolation on students and staff in higher education: A qualitative study. International Journal of Environmental Research and Public Health, 18(20), Article 10675. https://doi.org/10.3390/ijerph182010675

This qualitative study explored the impact of COVID-19 self-isolation and social restriction measures on university students, through the perspectives of both students and the staff supporting them. The study comprised 11 focus groups (students) and... Read More about Impacts of the COVID-19 pandemic and self-isolation on students and staff in higher education: A qualitative study.

Students’ views towards sars-cov-2 mass asymptomatic testing, social distancing and self-isolation in a university setting during the covid-19 pandemic: A qualitative study (2021)
Journal Article
Blake, H., Knight, H., Jia, R., Corner, J., Morling, J. R., Denning, C., …Vedhara, K. (2021). Students’ views towards sars-cov-2 mass asymptomatic testing, social distancing and self-isolation in a university setting during the covid-19 pandemic: A qualitative study. International Journal of Environmental Research and Public Health, 18(8), Article 4182. https://doi.org/10.3390/ijerph18084182

We aimed to explore university students’ perceptions and experiences of SARS-CoV-2 mass asymptomatic testing, social distancing and self-isolation, during the COVID-19 pandemic. This qualitative study comprised of four rapid online focus groups condu... Read More about Students’ views towards sars-cov-2 mass asymptomatic testing, social distancing and self-isolation in a university setting during the covid-19 pandemic: A qualitative study.

The use of fluorescence correlation spectroscopy to monitor cell surface ?2?adrenoceptors at low expression levels in human embryonic stem cell?derived cardiomyocytes and fibroblasts (2021)
Journal Article
Goulding, J., Kondrashov, A., Mistry, S. J., Melarangi, T., Vo, N. T. N., Hoang, D. M., …Hill, S. J. (2021). The use of fluorescence correlation spectroscopy to monitor cell surface β2‐adrenoceptors at low expression levels in human embryonic stem cell‐derived cardiomyocytes and fibroblasts. FASEB Journal, 35(4), Article e21398. https://doi.org/10.1096/fj.202002268r

The importance of cell phenotype in determining the molecular mechanisms underlying ?2- adrenoceptor (?2AR) function has been noted previously when comparing responses in primary cells and recombinant model cell lines. Here, we have generated haploty... Read More about The use of fluorescence correlation spectroscopy to monitor cell surface ?2?adrenoceptors at low expression levels in human embryonic stem cell?derived cardiomyocytes and fibroblasts.

Complex Relationship Between Cardiac Fibroblasts and Cardiomyocytes in Health and Disease (2021)
Journal Article
Hall, C., Gehmlich, K., Denning, C., & Pavlovic, D. (2021). Complex Relationship Between Cardiac Fibroblasts and Cardiomyocytes in Health and Disease. Journal of the American Heart Association, 10(5), Article e019338. https://doi.org/10.1161/jaha.120.019338

Cardiac fibroblasts are the primary cell type responsible for deposition of extracellular matrix in the heart, providing support to the contracting myocardium and contributing to a myriad of physiological signaling processes. Despite the importance o... Read More about Complex Relationship Between Cardiac Fibroblasts and Cardiomyocytes in Health and Disease.

Discovery of a Novel Polymer for Xeno-Free, Long-Term Culture of Human Pluripotent Stem Cell Expansion (2020)
Journal Article
Nasir, A., Thorpe, J., Burroughs, L., Meurs, J., Pijuan‐Galito, S., Irvine, D. J., …Denning, C. (2020). Discovery of a Novel Polymer for Xeno-Free, Long-Term Culture of Human Pluripotent Stem Cell Expansion. Advanced Healthcare Materials, 10(6), Article 2001448. https://doi.org/10.1002/adhm.202001448

Human pluripotent stem cells (hPSCs) can be expanded and differentiated in vitro into almost any adult tissue cell type, and thus have great potential as a source for cell therapies with biomedical application. In this study, a fully-defined polymer... Read More about Discovery of a Novel Polymer for Xeno-Free, Long-Term Culture of Human Pluripotent Stem Cell Expansion.