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All Outputs (22)

Screening Mutants by Single Fly Genomic PCR (2022)
Book Chapter
Nagarajan, U., & Georgiou, M. (2022). Screening Mutants by Single Fly Genomic PCR. In Notch Signaling Research (15-25). Humana Press. https://doi.org/10.1007/978-1-0716-2201-8_2

Imprecise P-element excision or FRT-mediated recombination is routinely performed to mutagenize a gene of interest. It is, however, tedious to maintain all independent and individual excised mutant fly lines before the presence of a mutation is confi... Read More about Screening Mutants by Single Fly Genomic PCR.

A Workflow to Track and Analyze Endothelial Migration During Vascular Development in Zebrafish Embryos Using Lightsheet Microscopy (2022)
Book Chapter
Wilkinson, R. N. (2022). A Workflow to Track and Analyze Endothelial Migration During Vascular Development in Zebrafish Embryos Using Lightsheet Microscopy. . Springer US. https://doi.org/10.1007/978-1-0716-2059-5_2

Zebrafish allow unrivalled in vivo imaging of vascular development due to their optical translucency and the availability of transgenic lines which fluorescently label cells and tissues of interest. Advances in light sheet fluorescence microscopy all... Read More about A Workflow to Track and Analyze Endothelial Migration During Vascular Development in Zebrafish Embryos Using Lightsheet Microscopy.

In Vivo cAMP Dynamics in Drosophila Larval Neurons (2022)
Book Chapter
Maiellaro, I. (2022). In Vivo cAMP Dynamics in Drosophila Larval Neurons. In M. Zaccolo (Ed.), cAMP Signaling: Methods and Protocols (181-194). New York: Humana Press. https://doi.org/10.1007/978-1-0716-2245-2_11

Cyclic adenosine monophosphate (cAMP) is a universal second messenger that mediates a myriad of cell functions across all kingdoms of life. The ability to monitor intracellular changes of cAMP concentration in living cells using FRET-based biosensors... Read More about In Vivo cAMP Dynamics in Drosophila Larval Neurons.

Immune-instructive materials and surfaces for medical applications (2021)
Book Chapter
Fisher, L. E., & Ghaemmaghami, A. M. (2021). Immune-instructive materials and surfaces for medical applications. In S. F. Badylak, & J. H. Elisseeff (Eds.), Immunomodulatory Biomaterials: Regulating the Immune Response with Biomaterials to Affect Clinical Outcome (67-87). Elsevier. https://doi.org/10.1016/B978-0-12-821440-4.00008-6

Biomaterials are an integral part of modern medicine with diverse applications including drug delivery platforms, scaffolds for tissue engineering, biosensors and medical devices/implants. The aging population and steady increase in the prevalence of... Read More about Immune-instructive materials and surfaces for medical applications.

Neuromolecular Mechanisms of Cannabis Action (2020)
Book Chapter
Adel, Y., & Alexander, S. P. (2021). Neuromolecular Mechanisms of Cannabis Action. In Cannabinoids and Neuropsychiatric Disorders (15-28). Springer International Publishing. https://doi.org/10.1007/978-3-030-57369-0_2

Most of our current understanding of the neuromolecular mechanisms of Cannabis action focusses on two plant cannabinoids, THC and CBD. THC acts primarily through presynaptic CB cannabinoid receptors to regulate neurotransmitter release in the brain,... Read More about Neuromolecular Mechanisms of Cannabis Action.

Measuring vaccine responses in the multiplex era (2018)
Book Chapter
Ayling, K., Vedhara, K., & Fairclough, L. (2018). Measuring vaccine responses in the multiplex era. In Q. Yan (Ed.), Psychoneuroimmunology: Methods and Protocols (327-340). (2nd). New York, USA: Springer Publishing Company. https://doi.org/10.1007/978-1-4939-7828-1_17

Vaccine studies in psychoneuroimmunology (PNI) provide an insight into biopsychosocial interactions and their role in infectious diseases. How to measure vaccine responses is therefore of critical importance for PNI researchers. In this chapter, trad... Read More about Measuring vaccine responses in the multiplex era.

Moonlighting Functions of Bacterial Fructose 1,6-Bisphosphate Aldolases (2017)
Book Chapter
Oldfield, N. J., Shams, F., Wooldridge, K. G., & Turner, D. P. (2017). Moonlighting Functions of Bacterial Fructose 1,6-Bisphosphate Aldolases. In B. Henderson (Ed.), Moonlighting Proteins: Novel Virulence Factors in Bacterial Infections (321-331). Wiley

© 2017 John Wiley & Sons, Inc. Moonlighting proteins are multifunctional proteins which perform two or more biochemical functions using a single polypeptide chain. Moonlighting proteins have been documented in diverse organisms, including archaea,... Read More about Moonlighting Functions of Bacterial Fructose 1,6-Bisphosphate Aldolases.

Transcriptional regulation and responses in filamentous fungi exposed to lignocellulose (2015)
Book Chapter
Daly, P., van Munster, J. M., Archer, D. B., & Raulo, R. (2015). Transcriptional regulation and responses in filamentous fungi exposed to lignocellulose. In R. N. Silva (Ed.), Mycology: current and future developments: fungal biotechnology for biofuel production. Bentham Science. https://doi.org/10.2174/97816810807411150101

Biofuels derived from lignocellulose are attractive alternative fuels but their production suffers from a costly and inefficient saccharification step that uses fungal enzymes. One route to improve this efficiency is to understand better the transcri... Read More about Transcriptional regulation and responses in filamentous fungi exposed to lignocellulose.

Biomimetic Human Lung Models (2014)
Book Chapter
Harrington, H. C., Cato, P. A., & Ghaemmaghami, A. M. (2014). Biomimetic Human Lung Models. In Handbook of Biomimetics and Bioinspiration (1017-1036). World Scientific. https://doi.org/10.1142/9789814354936_0037

New strategies for the treatment of lung disease are being sought to combat the increased prevalence of lung disease within the developedworld.As researchmoves away from reliance upon the use of animal models in pre-clinical studies innovative method... Read More about Biomimetic Human Lung Models.

Front matter (2014)
Book Chapter
Jabbari, E., Kim, D. H., Lee, L. P., Ghaemmaghami, A., & Khademhosseini, A. (2014). Front matter. In E. Jabbari, D. Ho Kim, L. P. Lee, A. Ghaemmaghami, & A. Khademhosseini (Eds.), Handbook of Biomimetics and Bioinspiration: Biologically-driven Engineering of Materials, Processes, Devices, and Systems (i-lix). World Scientific. https://doi.org/10.1142/9789814354936_fmatter01

LC-MS/MS Quantitative Analysis of Quorum Sensing Signal Molecules (2014)
Book Chapter
Ortori, C. A., Ortori, C., Halliday, N., Cámara, M., Williams, P., & Barrett, D. A. (2014). LC-MS/MS Quantitative Analysis of Quorum Sensing Signal Molecules. In A. Filloux, & J. Ramos (Eds.), Pseudomonas Methods and Protocols (255-270). Springer. https://doi.org/10.1007/978-1-4939-0473-0_21

© Springer Science+Business Media New York 2014. Extracts taken from spent growth media from Pseudomonas aeruginosa can be analyzed for N -acyl- L - homoserine lactones and 2-alkyl-4-(1 H)-quinolones (AQs), including the known quorum sensing signalli... Read More about LC-MS/MS Quantitative Analysis of Quorum Sensing Signal Molecules.

Imaging Angiogenesis, Inflammation, and Metastasis in the Tumor Microenvironment with Magnetic Resonance Imaging (2013)
Book Chapter
O’Brien, E. R., Serres, S., & Sibson, N. R. (2013). Imaging Angiogenesis, Inflammation, and Metastasis in the Tumor Microenvironment with Magnetic Resonance Imaging. In Tumor Microenvironment and Cellular Stress (263-283). Springer New York. https://doi.org/10.1007/978-1-4614-5915-6_12

With the development of new imaging techniques, the potential for probing the molecular, cellular, and structural components of the tumor microenvironment in situ has increased dramatically. A multitude of imaging modalities have been successfully em... Read More about Imaging Angiogenesis, Inflammation, and Metastasis in the Tumor Microenvironment with Magnetic Resonance Imaging.

Cellular and Viral Mechanisms of HIV-1 Transmission Mediated by Dendritic Cells (2012)
Book Chapter
Coleman, C. M., St.Gelais, C., & Wu, L. (2012). Cellular and Viral Mechanisms of HIV-1 Transmission Mediated by Dendritic Cells. Springer New York. https://doi.org/10.1007/978-1-4614-4433-6_4

Dendritic cells (DCs) play a key role in the initial infection and cell-to-cell transmission events that occur upon HIV-1 infection. DCs interact closely with CD4+ T cells, the main target of HIV-1 replication. HIV-1 challenged DCs and target CD4+ T... Read More about Cellular and Viral Mechanisms of HIV-1 Transmission Mediated by Dendritic Cells.

Hormone transport (2010)
Book Chapter
Kerr, I. D., Carrier, D. J., & Twycross, J. (2010). Hormone transport. In The Plant Plasma Membrane, (379-397). Springer. https://doi.org/10.1007/978-3-642-13431-9_17

Hormone transport in plants is a fundamental process governing all aspects of plant development. Identification and functional analysis of plasma membrane proteins, which regulate the import and export of plant hormones, is a requirement if we are to... Read More about Hormone transport.

Heterobranchia II: The Pulmonata (2008)
Book Chapter
Mordan, P., & Wade, C. (2008). Heterobranchia II: The Pulmonata. In W. Ponder (Ed.), Phylogeny and Evolution of the Mollusca (409-426). University of California Press. https://doi.org/10.1525/california/9780520250925.003.0015

This chapter focuses on the Pulmonata, gastropods belonging to the clade Heterobranchia. It explains that the pulmonates include the large majority of land snails and slugs, as well as many freshwater snails and a few small families of estuarine and... Read More about Heterobranchia II: The Pulmonata.

Cloning, Expression, and Functional Analysis of Patient-Derived Hepatitis C Virus Glycoproteins (2007)
Book Chapter
Tarr, A. W., Owsianka, A. M., Szwejk, A., Ball, J. K., & Patel, A. H. (2007). Cloning, Expression, and Functional Analysis of Patient-Derived Hepatitis C Virus Glycoproteins. In Glycovirology Protocols (177-197). Humana Press. https://doi.org/10.1385/1-59745-393-5%3A177

Hepatitis C virus (HCV) infection is a major cause of severe chronic liver disease including cirrhosis and hepatocellular carcinoma. HCV has been classified into six major genotypes that exhibit extensive genetic variability, particularly in the enve... Read More about Cloning, Expression, and Functional Analysis of Patient-Derived Hepatitis C Virus Glycoproteins.

Cloning, expression, and functional analysis of patient-derived hepatitis C virus glycoproteins (2007)
Book Chapter
Tarr, A. W., Owsianka, A. M., Szwejk, A., Ball, J. K., & Patel, A. H. (2007). Cloning, expression, and functional analysis of patient-derived hepatitis C virus glycoproteins. In R. J. Sugrue (Ed.), Glycovirology Protocols (177-197). Humana Press. https://doi.org/10.1007/978-1-59745-393-6_13

Hepatitis C virus (HCV) infection is a major cause of severe chronic liver disease including cirrhosis and hepatocellular carcinoma. HCV has been classified into six major genotypes that exhibit extensive genetic variability, particularly in the enve... Read More about Cloning, expression, and functional analysis of patient-derived hepatitis C virus glycoproteins.

Cloning, expression, and functional analysis of patient-derived hepatitis C virus glycoproteins (2007)
Book Chapter
Tarr, A. W., Owsianka, A. M., Szwejk, A., Ball, J. K., & Patel, A. H. (2007). Cloning, expression, and functional analysis of patient-derived hepatitis C virus glycoproteins. In R. J. Sugrue (Ed.), Glycovirology Protocols (177-197). Humana Press. https://doi.org/10.1007/978-1-59745-393-6_13

Hepatitis C virus (HCV) infection is a major cause of severe chronic liver disease including cirrhosis and hepatocellular carcinoma. HCV has been classified into six major genotypes that exhibit extensive genetic variability, particularly in the enve... Read More about Cloning, expression, and functional analysis of patient-derived hepatitis C virus glycoproteins.