Research Repository

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The chemical throwing power of lithium-based inhibitors from organic coatings on AA2024-T3 (2019)
Journal Article
Visser, P., Marcoen, K., Trindade, G. F., Abel, M., Watts, J. F., Hauffman, T., …Terryn, H. (in press). The chemical throwing power of lithium-based inhibitors from organic coatings on AA2024-T3. Corrosion Science,

Chemical throwing power, being the distance over which an inhibitor is able to protect a defect effectively, is an important parameter for active protective coatings. This study investigates the chemical throwing power of lithium-based leachable corr... Read More

Synthesis of methacrylate-terminated block copolymers with reduced transesterification by controlled ring-opening polymerization (2018)
Journal Article
Ruiz-Cantu, L. A., Pearce, A. K., Burroughs, L., Bennett, T. M., Vasey, C. E., Wildman, R., …Taresco, V. (2019). Synthesis of methacrylate-terminated block copolymers with reduced transesterification by controlled ring-opening polymerization. Macromolecular Chemistry and Physics, 220(2), doi:10.1002/macp.201800459

This work presents a robust method to achieve the synthesis of low molecular weight polyesters 26 via ring-opening polymerization (ROP) initiated by 2-hydroxyethyl-methacrylate (HEMA) 27 when using triazabicyclodecene (TBD) as catalyst. The effect th... Read More

Measurement of the total angiotensinogen and its reduced and oxidised forms in human plasma using targeted LC-MS/MS (2018)
Journal Article
Dahabiyeh, L. A., Tooth, D., Carrell, R. W., Read, R. J., Yan, Y., Pipkin, F. B., & Barrett, D. A. (2019). Measurement of the total angiotensinogen and its reduced and oxidised forms in human plasma using targeted LC-MS/MS. Analytical and Bioanalytical Chemistry, 411(2), 427–437. doi:10.1007/s00216-018-1455-2

Angiotensinogen (AGT) is a critical protein in the renin-angiotensin-aldosterone system and may have an important role in the pathogenesis of pre-eclampsia. The disulphide linkage between cysteines 18 and 138 has a key role in the redox switch of AGT... Read More

Changes in the testing for and incidence of coeliac disease in the UK 2005-2015: a population based cohort study (2018)
Journal Article
West, J., Otete, H., Sultan, A. A., & Crooks, C. J. (in press). Changes in the testing for and incidence of coeliac disease in the UK 2005-2015: a population based cohort study. Epidemiology,

The diagnosis rates of coeliac disease differ substantially between countries. Intriguingly, there has been recent evidence from Olmstead County, USA and Finland that in the last 5-10 years incidence has plateaued or even declined. In most population... Read More

The central region of CNOT1 and CNOT9 stimulate deadenylation by the Ccr4 Not nuclease module (2018)
Journal Article
Pavanello, L., Hall, B., Airhihen, B., & Winkler, G. S. (2018). The central region of CNOT1 and CNOT9 stimulate deadenylation by the Ccr4 Not nuclease module. Biochemical Journal, 475(21), 3437–3450. doi:10.1042/bcj20180456

Regulated degradation of cytoplasmic mRNA is important for the accurate execution of gene expression programmes in eukaryotic cells. A key step in this process is the shortening and removal of the mRNA poly(A) tail, which can be achieved by the recru... Read More

Rapid and accurate analysis of stem cell-derived extracellular vesicles with super resolution microscopy and live imaging (2018)
Journal Article
Nizamudeen, Z., Markus, R., Lodge, R., Parmenter, C., Platt, M., Chakrabarti, L., & Sottile, V. (2018). Rapid and accurate analysis of stem cell-derived extracellular vesicles with super resolution microscopy and live imaging. Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1865(12), 1891-1900. doi:10.1016/j.bbamcr.2018.09.008

Extracellular vesicles (EVs) have prevalent roles in cancer biology and regenerative medicine. Conventional techniques for characterising EVs including electron microscopy (EM), nanoparticle tracking analysis (NTA) and tuneable resistive pulse sensin... Read More

Photocrosslinkable gelatin hydrogels modulate the production of the major pro-inflammatory cytokine, TNF-α, by human mononuclear cells (2018)
Journal Article
Donaldson, A. R., Edi Tanase, C., Awuah, D., Vasanthi Bathri Narayanan, P., Hall, L., Nikkhah, M., …Ghaemmaghami, A. M. (2018). Photocrosslinkable gelatin hydrogels modulate the production of the major pro-inflammatory cytokine, TNF-α, by human mononuclear cells. Frontiers in Bioengineering and Biotechnology, 6, doi:10.3389/fbioe.2018.00116. ISSN 2296-4185

Hydrogels are an attractive class of biomaterials in tissue engineering due to their inherently compatible properties for cell culture. Gelatin methacryloyl (GelMA) has shown significant promise in the fields of tissue engineering and drug delivery,... Read More

Analytical ultracentrifugation in saliva research: Impact of green tea astringency and its significance on the in-vivo aroma release (2018)
Journal Article
Dinu, V., Liu, C., Ali, J., Ayed, C., Gershkovich, P., Adams, G. G., …Fisk, I. (2018). Analytical ultracentrifugation in saliva research: Impact of green tea astringency and its significance on the in-vivo aroma release. Scientific Reports, 8, (1-9). doi:10.1038/s41598-018-31625-w

Current saliva testing methods rely on cutting edge yet expensive techniques for the detection and analysis of genetic material, proteins and biomarkers for clinical use. However, these techniques are limited in scope and often cannot be used with co... Read More

Autophagy modulation: a prudent approach in cancer treatment? (2018)
Journal Article
Bishop, E., & Bradshaw, T. D. (2018). Autophagy modulation: a prudent approach in cancer treatment?. Cancer Chemotherapy and Pharmacology, doi:10.1007/s00280-018-3669-6. ISSN 0344-5704

Autophagy is a tightly controlled process comprising lysosomal degradation and recycling of cellular proteins and organelles. In cancer, its paradoxical dual role of cytoprotection and cytotoxicity is context-dependent and controversial. Autophagy pr... Read More

Shear bands and the evolving microstructure in a drying colloidal film studied with scanning μ-SAXS (2018)
Journal Article
Yang, B., Smith, N. D., Johannes, A., Burghammer, M., & Smith, M. I. (2018). Shear bands and the evolving microstructure in a drying colloidal film studied with scanning μ-SAXS. Scientific Reports, 8, doi:10.1038/s41598-018-31405-6. ISSN 2045-2322

Shear localisation in thin bands is an important process involved in the plastic deformation of materials subject to stress. This process is often sensitive to the sample microstructure (amorphous / crystalline). Here we show using the scanning μ-SAX... Read More

Assessment of the cellular localisation of the Annexin A2/S100A10 complex in human placenta (2018)
Journal Article
Seham, E., & Lodewijk, D. (2018). Assessment of the cellular localisation of the Annexin A2/S100A10 complex in human placenta. Journal of Molecular Histology, doi:10.1007/s10735-018-9791-2

The AnxA2/S100A10 complex has been implicated in various placental functions but although the localisation of these proteins individually has been studied, there is no information about the localisation of their complex in situ at the cellular level.... Read More

Evaluation of extraction and normalisation strategies for the analysis of lipids in placental vessels (2018)
Journal Article
Sander, K. N., Hay, D. P., Broughton-Pipkin, F., Khan, R. N., & Barrett, D. A. (2018). Evaluation of extraction and normalisation strategies for the analysis of lipids in placental vessels. Journal of Pharmaceutical and Biomedical Analysis, doi:10.1016/j.jpba.2018.08.012. ISSN 0731-7085

The analysis of lipids in tough or fibrous biological tissues can be challenging due to difficulties in obtaining a representative sample following homogenisation of the tissue. Furthermore, the choice of normalisation method can have a major effect... Read More

Therapeutic exploitation of IPSE, a urogenital parasite-derived host modulatory protein, for chemotherapy-induced hemorrhagic cystitis (2018)
Journal Article
Mbanefo, E. C., Le, L., Pennington, L. F., Odegaard, J. I., Jardetzky, T. S., Alouffi, A., …Hsieh, M. H. (2018). Therapeutic exploitation of IPSE, a urogenital parasite-derived host modulatory protein, for chemotherapy-induced hemorrhagic cystitis. FASEB Journal, 32(8), (4408-4419). doi:10.1096/fj.201701415r. ISSN 0892-6638

Chemotherapy-induced hemorrhagic cystitis (CHC) can be difficult to manage. Prior work suggests that IL-4 alleviates ifosfamide-induced hemorrhagic cystitis (IHC), but systemically administered IL-4 causes significant side effects. We hypothesized th... Read More

The Meisenheimer complex as a paradigm in drug discovery: reversible covalent inhibition through C67 of the ATP binding site of PLK1 (2018)
Journal Article
Pearson, R. J., Blake, D. G., Mezna, M., Fischer, P. M., Westwood, N. J., & McInnes, C. (2018). The Meisenheimer complex as a paradigm in drug discovery: reversible covalent inhibition through C67 of the ATP binding site of PLK1. Cell Chemical Biology, doi:10.1016/j.chembiol.2018.06.001

The polo kinase family are important oncology targets that act in regulating entry into and progression through mitosis. Structure-guided discovery of a new class of inhibitors of Polo-like kinase 1 (PLK1) catalytic activity that interact with Cys67... Read More

Transcriptional analysis of adipose tissue during development reveals depot-specific responsiveness to maternal dietary supplementation (2018)
Journal Article
Fainberg, H. P., Birtwistle, M., Alagal, R., Alhaddad, A., Pope, M., Davies, G., …Symonds, M. E. (2018). Transcriptional analysis of adipose tissue during development reveals depot-specific responsiveness to maternal dietary supplementation. Scientific Reports, 8(1), (1-11). doi:10.1038/s41598-018-27376-3

Brown adipose tissue (BAT) undergoes pronounced changes after birth coincident with the loss of the BAT-specifc uncoupling protein (UCP)1 and rapid fat growth. The extent to which this adaptation may vary between anatomical locations remains unknown,... Read More

Polyionic tags as enhancers of protein solubility in recombinant protein expression (2018)
Journal Article
Paraskevopoulou, V., & Falcone, F. (2018). Polyionic tags as enhancers of protein solubility in recombinant protein expression. Microorganisms, 6(2), 47. doi:10.3390/microorganisms6020047

Since the introduction of recombinant protein expression in the second half of the 1970s, the growth of the biopharmaceutical field has been rapid and protein therapeutics has come to the foreground. Biophysical and structural characterisation of rec... Read More

Odontogenic differentiation of human dental pulp stem cells on hydrogel scaffolds derived from decellularized bone extracellular matrix and collagen type I (2016)
Journal Article
Paduano, F., Marrelli, M., White, L. J., Shakesheff, K., & Tatullo, M. (2016). Odontogenic differentiation of human dental pulp stem cells on hydrogel scaffolds derived from decellularized bone extracellular matrix and collagen type I. PLoS ONE, 11(2), 1-18. doi:10.1371/journal.pone.0148225

Objectives The aim of this study was to evaluate the level of odontogenic differentiation of dental pulp stem cells (DPSCs) on hydrogel scaffolds derived from bone extracellular matrix (bECM) in comparison to those seeded on collagen I (Col-I), on... Read More

Synthesis of 19F nucleic acid–polymer conjugates as real-time MRI probes of biorecognition (2016)
Journal Article
Sicilia, G., Davis, A. L., Spain, S. G., Magnusson, J. P., Boase, N. R., Thurecht, K. J., & Alexander, C. (2016). Synthesis of 19F nucleic acid–polymer conjugates as real-time MRI probes of biorecognition. Polymer Chemistry, 7(12), 2180-2191. doi:10.1039/C5PY01883H

Polymer–DNA conjugates in which one nucleic acid strand contains fluorine-substituted nucleobases have been prepared and characterised. The efficacy of these novel 19F nucleic acid–polymer conjugates as sensitive and selective in vitro reporters of D... Read More

Suspended planar-array chips for molecular multiplexing at the microscale (2015)
Journal Article
Torras, N., Agusil, J. P., Vázquez, P., Duch, M., Hernández-Pinto, A. M., Samitier, J., …Plaza, J. A. (2016). Suspended planar-array chips for molecular multiplexing at the microscale. Advanced Materials, 28(7), (1449-1454). doi:10.1002/adma.201504164. ISSN 0935-9648

A novel suspended planar‐array chips technology is described, which effectively allows molecular multiplexing using a single suspended chip to analyze extraordinarily small volumes. The suspended chips are fabricated by combining silicon‐based techno... Read More

Live imaging of cellular internalization of single colloidal particle by combined label-free and fluorescence total internal reflection microscopy (2015)
Journal Article
Byrne, G. D., Vllasaliu, D., Falcone, F. H., Somekh, M. G., & Stolnik, S. (2015). Live imaging of cellular internalization of single colloidal particle by combined label-free and fluorescence total internal reflection microscopy. Molecular Pharmaceutics, 12(11), 3862-3870. doi:10.1021/acs.molpharmaceut.5b00215

In this work we utilize the combination of label-free total internal reflection microscopy and total internal reflectance fluorescence (TIRM/TIRF) microscopy to achieve a simultaneous, live imaging of single, label-free colloidal particle endocytosis... Read More