Research Repository

See what's under the surface


Quantitative RT-PCR assays for identification and typing of the Equine encephalosis virus (2018)
Journal Article
Maan, S., Belaganahalli, M. N., Maan, N. S., Potgieter, A. C., & Mertens, P. P. C. (2019). Quantitative RT-PCR assays for identification and typing of the Equine encephalosis virus. Brazilian Journal of Microbiology, 50(1), 287-296. doi:10.1007/s42770-018-0034-1

Equine encephalosis (EE) is an acute, arthropod-borne, noncontagious, febrile disease of equids. The clinical signs of EE are similar to milder forms of African horse sickness (AHS) and the two diseases can be easily confused. The Equine encephalosis... Read More

Transcriptomic insights into the early host-pathogen interaction of cat intestine with Toxoplasma gondii (2018)
Journal Article
Wang, M., Zhang, F., Elsheikha, H. M., Zhang, N., He, J., Luo, J., & Zhu, X. (2018). Transcriptomic insights into the early host-pathogen interaction of cat intestine with Toxoplasma gondii. Parasites and Vectors, 11(1), doi:10.1186/s13071-018-3179-8

Background: Although sexual reproduction of the parasite Toxoplasma gondii exclusively occurs in the cat intestine, knowledge about the alteration of gene expression in the intestine of cats infected with T. gondii is still limited. Here, we investig... Read More

Reviving phage therapy for the treatment of cholera (2018)
Journal Article
Bhandare, S., Colom, J., Baig, A., Ritchie, J. M., Bokhari, H., Shah, M. A., …Atterbury, R. J. (2019). Reviving phage therapy for the treatment of cholera. Journal of Infectious Diseases, 219(5), 786–794. doi:10.1093/infdis/jiy563

Cholera remains a major risk in developing countries, particularly after natural or man-made disasters. Vibrio cholerae El Tor is the most important cause of these outbreaks, and is becoming increasingly resistant to antibiotics, so alternative thera... Read More

Use of big data in cattle practice (2018)
Journal Article
Hudson, C., Kaler, J., & Down, P. (2018). Use of big data in cattle practice. In Practice, 40(9), 396-410. doi:10.1136/inp.k4328

The concept of big data, associated data sources and analytics is becoming increasingly talked about both in society as a whole and within the livestock industry. This article provides a clinician-focused review of what big data means, how it is alre... Read More

Survey-based pilot study into the chosen therapy and prophylaxis used by UK primary care veterinary surgeons against canine angiostrongylosis (2018)
Journal Article
Shuttleworth, A., Dunning, M., Wright, I., & Elsheikha, H. M. (2018). Survey-based pilot study into the chosen therapy and prophylaxis used by UK primary care veterinary surgeons against canine angiostrongylosis. Veterinary parasitology (Amsterdam), 14, 144-149. doi:10.1016/j.vprsr.2018.10.010

Canine Angiostrongylosis (CA), a gastropod-borne parasitic infection caused by the metastrongyloid nematode Angiostrongylus vasorum, is an important cause of significant morbidity to domestic dogs across the UK as well as in other European countries.... Read More

Implications of shunt morphology for the surgical management of extrahepatic portosystemic shunts (2018)
Journal Article
White, R., Parry, A., & Shales, C. (2018). Implications of shunt morphology for the surgical management of extrahepatic portosystemic shunts. Australian Veterinary Journal, 96(11), 433-441. doi:10.1111/avj.12756

Objectives: To describe the implications of extrahepatic portosystemic shunt morphology for 14 the chosen site of shunt closure in dogs and cats. Methods: A retrospective review of a consecutive series of dogs and cats managed for 16 congenital ext... Read More

Communication of prostate cancer cells with bone cells via extracellular vesicle RNA: a potential mechanism of metastasis (2018)
Journal Article
Probert, C., Dottorini, T., Speakman, A., Hunt, S., Nafee, T., Fazeli, A., …James, V. (2018). Communication of prostate cancer cells with bone cells via extracellular vesicle RNA: a potential mechanism of metastasis. Oncogene, 38, 1751–1763. doi:10.1038/s41388-018-0540-5

The role of extracellular vesicles (EVs) as vehicles for cell-to-cell communication between a tumour and its environment is a relatively new concept. The hypothesis that EVs may be critical in co-opting tissues by tumours to generate distant metastat... Read More

Acute Toxoplasma gondii infection in cats induced tissue-specific transcriptional response dominated by immune signatures (2018)
Journal Article
Cong, W., Dottorini, T., Khan, F., Emes, R. D., Zhang, F., Zhou, C., …Zhu, X. (2018). Acute Toxoplasma gondii infection in cats induced tissue-specific transcriptional response dominated by immune signatures. Frontiers in Immunology, 9, doi:10.3389/fimmu.2018.02403. ISSN 1664-3224

RNA-sequencing was used to detect transcriptional changes in six tissues of cats, seven days after T. gondii infection. A total of 737 genes were differentially expressed (DEGs), of which 410 were up-regulated and 327 were down-regulated. The liver e... Read More

Associations between routinely collected dairy herd improvement data and insemination outcome in UK dairy herds (2018)
Journal Article
HUDSON, C., & GREEN, M. (2018). Associations between routinely collected dairy herd improvement data and insemination outcome in UK dairy herds. Journal of Dairy Science, 101(12), 11262-11274. doi:10.3168/jds.2017-13962

Milk constituent concentrations in samples taken during early lactation are often used to generate proxy measures for energy balance in dairy herds. This study aimed to explore associations between these and other measures routinely recorded by dairy... Read More

Clinical audit in farm animal veterinary practice: Part 1: preparing for audit (2018)
Journal Article
Waine, K., Dean, R., Hudson, C., & Brennan, M. (2018). Clinical audit in farm animal veterinary practice: Part 1: preparing for audit. In Practice, 40(8), 360-364. doi:10.1136/inp.k3950

This article, the first in a two-part series providing practical advice for practitioners on carrying out clinical audits, discusses the preparation and initiation of clinical audit in farm animal practice. In the second article, to be published in a... Read More

Recent changes in infectious diseases in European wildlife (2018)
Journal Article
Yon, L., Duff, J. P., Ågren, E. O., Erdélyi, K., Ferroglio, E., Godfroid, J., …Gavier-Widén, D. (2019). Recent changes in infectious diseases in European wildlife. Journal of Wildlife Diseases, 55(1), 3-43. doi:10.7589/2017-07-172

Many infectious diseases originating from, or carried by, wildlife affect wildlife conservation and biodiversity, livestock health, or human health. We provide an update on changes in the epidemiology of 25 selected infectious, wildlife-related disea... Read More

Rapid and accurate analysis of stem cell-derived extracellular vesicles with super resolution microscopy and live imaging (2018)
Journal Article
Nizamudeen, Z., Markus, R., Lodge, R., Parmenter, C., Platt, M., Chakrabarti, L., & Sottile, V. (2018). Rapid and accurate analysis of stem cell-derived extracellular vesicles with super resolution microscopy and live imaging. Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1865(12), 1891-1900. doi:10.1016/j.bbamcr.2018.09.008

Extracellular vesicles (EVs) have prevalent roles in cancer biology and regenerative medicine. Conventional techniques for characterising EVs including electron microscopy (EM), nanoparticle tracking analysis (NTA) and tuneable resistive pulse sensin... Read More

A cross-sectional study of experiences and attitudes towards clinical audit of farm animal veterinary surgeons in the United Kingdom (2018)
Journal Article
Waine, K., Dean, R., Hudson, C., Huxley, J., & Brennan, M. (2018). A cross-sectional study of experiences and attitudes towards clinical audit of farm animal veterinary surgeons in the United Kingdom. Veterinary Sciences, 5, (84). doi:10.3390/vetsci5040084. ISSN 2306-7381

Clinical audit is a quality improvement tool used to assess and improve the clinical services provided to patients. This is the first study to investigate the extent to which clinical audit is understood and utilised in farm animal veterinary practic... Read More

A survey to determine the farm level impact of Schmallenberg virus during the 2016-17 UK lambing season (2018)
Journal Article
Stokes, J. E., Tarlinton, R. E., Lovatt, F., Baylis, M., Carson, A., & Duncan, J. S. (2018). A survey to determine the farm level impact of Schmallenberg virus during the 2016-17 UK lambing season. Veterinary Record, doi:10.1136/vr.104866. ISSN 0042-4900

Schmallenberg virus (SBV) causes abortions, still births and fetal malformations in naive ruminants. The impact of the initial outbreak (2011/2012) on British sheep farms has been previously investigated, with higher farmer perceived impacts and incr... Read More

Avian Cardiovascular Disease Characteristics, Causes and Genomics (2018)
Book
Kubale, V., Merry, K., Miller, G., Ruiz Diaz, M., & Rutland, C. (2018). Avian Cardiovascular Disease Characteristics, Causes and Genomics. In X. Liu (Ed.), Application of genetics and genomics in poultry science, 141-162. London: InTech. doi:10.5772/intechopen.78005

Cardiovascular disease is common in avian species and increasing commercial economic losses and demand for healthcare in the household/smallholding veterinary sector has resulted in increased research into these disorders. This in turn has highlighte... Read More

Metallome of cerebrovascular endothelial cells infected with Toxoplasma gondii using μ-XRF imaging and inductively coupled plasma mass spectrometry (2018)
Journal Article
Al-sandaqchi, A. T., Brignell, C., Collingwood, J. F., Geraki, K., Mirkes, E. M., Kong, K., …Elsheikha, H. M. (2018). Metallome of cerebrovascular endothelial cells infected with Toxoplasma gondii using μ-XRF imaging and inductively coupled plasma mass spectrometry. Metallomics, 1401-1414 . doi:10.1039/C8MT00136G

In this study, we measured the levels of elements in human brain microvascular endothelial cells (ECs) infected with T. gondii. ECs were infected with tachyzoites of the RH strain, and at 6, 24, and 48 hours post infection (hpi), the intracellular co... Read More

Gene therapy using plasmid DNA encoding VEGF164 and FGF2 genes: a novel treatment of naturally occurring tendinitis and desmitis in horses (2018)
Journal Article
Kovac, M., Litvin, Y. A., Aliev, R. O., Zakirova, E. Y., Rutland, C. S., Kiyasov, A. P., & Rizvanov, A. A. (2018). Gene therapy using plasmid DNA encoding VEGF164 and FGF2 genes: a novel treatment of naturally occurring tendinitis and desmitis in horses. Frontiers in Pharmacology, 9, doi:10.3389/fphar.2018.00978. ISSN 1663-9812

This clinical study describes the intralesional application of the plasmid DNA encoding two therapeutic species-specific growth factors: vascular endothelial growth factor (VEGF164) and fibroblast growth factor 2 (FGF2) in seven horses to restore nat... Read More

Prevalence of antibodies against Toxoplasma gondii in pets and their owners in Shandong province, Eastern China (2018)
Journal Article
Cong, W., Elsheikha, H. M., Zhou, N., Peng, P., Qin, S., Meng, Q., & Qian, A. (2018). Prevalence of antibodies against Toxoplasma gondii in pets and their owners in Shandong province, Eastern China. BMC Infectious Diseases, 18(1), doi:10.1186/s12879-018-3307-2

Background: Pet ownership in China has been steadily increasing over recent years. However, the risk of pet-associated zoonotic infection with the protozoan parasite Toxoplasma gondii remains poorly defined. Methods: In a cross-sectional survey, we... Read More

Functional characterization of dense granule proteins in Toxoplasma gondii RH strain using CRISPR-Cas9 system (2018)
Journal Article
Bai, M., Wang, J., Elsheikha, H. M., Liang, Q., Chen, K., Nie, L., & Zhu, X. (2018). Functional characterization of dense granule proteins in Toxoplasma gondii RH strain using CRISPR-Cas9 system. Frontiers in Cellular and Infection Microbiology, 8, (1-9). doi:10.3389/fcimb.2018.00300. ISSN 2235-2988

Infection with the apicomplexan protozoan parasite Toxoplasma gondii is an ongoing public health problem. The parasite's ability to invade and replicate within the host cell is dependent on many effectors, such as dense granule proteins (GRAs) releas... Read More

CARM1 (PRMT4) acts as a transcriptional coactivator during retinoic acid-induced embryonic stem cell differentiation (2018)
Journal Article
Quintero, C. M., Laursen, K. B., Mongan, N. P., Luo, M., & Gudas, L. J. (2018). CARM1 (PRMT4) acts as a transcriptional coactivator during retinoic acid-induced embryonic stem cell differentiation. Journal of Molecular Biology, 430(21), 4168-4182. doi:10.1016/j.jmb.2018.08.014

Activation of the retinoic acid (RA) signaling pathway is important for controlling embryonic stem cell differentiation and development. Modulation of this pathway occurs through the recruitment of different epigenetic regulators at the retinoic acid... Read More