All Outputs (81)

Associations between genomic stratification of breast cancer and centrally reviewed tumour pathology in the METABRIC cohort (2018)
Journal Article
Mukherjee, A., Russell, R., Chin, S., Liu, B., Rueda, O., Ali, H., …Provenzano, E. (2018). Associations between genomic stratification of breast cancer and centrally reviewed tumour pathology in the METABRIC cohort. npj Breast Cancer, 4(1), Article 5. https://doi.org/10.1038/s41523-018-0056-8

The integration of genomic and transcriptomic profiles of 2000 breast tumours from the METABRIC [Molecular Taxonomy of Breast Cancer International Consortium] cohort revealed ten subtypes, termed integrative clusters (IntClust/s), characterised by di... Read More about Associations between genomic stratification of breast cancer and centrally reviewed tumour pathology in the METABRIC cohort.

Water-soluble substituted chitosan derivatives as technology platform for inhalation delivery of siRNA (2018)
Journal Article
Capel, V., Vllasaliu, D., Watts, P., Clarke, P. A., Luxton, D., Grabowska, A. M., …Stolnik, S. (in press). Water-soluble substituted chitosan derivatives as technology platform for inhalation delivery of siRNA. Drug Delivery, 25(1), https://doi.org/10.1080/10717544.2018.1440668

Despite research efforts full potential of siRNA-based therapeutics has not yet been fully realized due to a need for suitable, effective delivery formulations. Here, we examine a potential of a new class of water-soluble chitosans as siRNA platform... Read More about Water-soluble substituted chitosan derivatives as technology platform for inhalation delivery of siRNA.

Pathways of cellular internalisation of liposomes delivered siRNA and effects on siRNA engagement with target mRNA and silencing in cancer cells (2018)
Journal Article
Alshehri, A. A., Grabowska, A. M., & Stolnik, S. (in press). Pathways of cellular internalisation of liposomes delivered siRNA and effects on siRNA engagement with target mRNA and silencing in cancer cells. Scientific Reports, 8(1), https://doi.org/10.1038/s41598-018-22166-3

Design of an efficient delivery system is a generally recognised bottleneck in translation of siRNA technology into clinic. Despite research efforts, cellular processes that determine efficiency of siRNA silencing achieved by different delivery formu... Read More about Pathways of cellular internalisation of liposomes delivered siRNA and effects on siRNA engagement with target mRNA and silencing in cancer cells.

A HIF-independent, CD133-mediated mechanism of cisplatin resistance in glioblastoma cells (2018)
Journal Article
Ahmed, E. M., Bandopadhyay, G., Coyle, B., & Grabowska, A. M. (2018). A HIF-independent, CD133-mediated mechanism of cisplatin resistance in glioblastoma cells. Cellular Oncology, 41(3), https://doi.org/10.1007/s13402-018-0374-8

Purpose Glioblastoma Multiforme (GBM) is the commonest brain tumour in adults. A population of cells, known as cancer stem cells (CSCs), is thought to mediate chemo/radiotherapy resistance. CD133 is a cell surface marker to identify and isolate... Read More about A HIF-independent, CD133-mediated mechanism of cisplatin resistance in glioblastoma cells.

Ponatinib for Treating Chronic Myeloid Leukaemia: An Evidence Review Group Perspective of a NICE Single Technology Appraisal (2018)
Journal Article
Pandor, A., Stevenson, M., Stevens, J., James, M. M., Hamilton, J., Byrne, J., …Wong, R. (2018). Ponatinib for Treating Chronic Myeloid Leukaemia: An Evidence Review Group Perspective of a NICE Single Technology Appraisal. PharmacoEconomics, 36(8), 903-915. https://doi.org/10.1007/s40273-018-0627-4

As part of its single technology appraisal process, the National Institute for Health and Care Excellence (NICE) invited the company that manufactures ponatinib (Inclusig®; Incyte Corporation) to submit evidence for the clinical and cost effectivenes... Read More about Ponatinib for Treating Chronic Myeloid Leukaemia: An Evidence Review Group Perspective of a NICE Single Technology Appraisal.

N_LyST: a simple and rapid screening test for Lynch Syndrome (2018)
Journal Article
Susanti, S., Fadhil, W., Ebili, H. O., Asiri, A., Nestarenkaite, A., Hadjimichael, E., …Ilyas, M. (2018). N_LyST: a simple and rapid screening test for Lynch Syndrome. Journal of Clinical Pathology, 71(8), 713-720. https://doi.org/10.1136/jclinpath-2018-205013

Aims: We sought to use PCR followed by high-resolution melting (HRM) analysis to develop a single closed-tube screening panel to screen for Lynch Syndrome. This comprises tests for microsatellite instability (MSI), MLH1 methylation promoter and BRAF... Read More about N_LyST: a simple and rapid screening test for Lynch Syndrome.

Phosphorylation of PRH/HHEX by Protein Kinase CK2 regulates cell proliferation and cell migration in diverse cell types (2018)
Book Chapter
Jayaraman, P., Wadey, K., George, S., & Gaston, K. (2018). Phosphorylation of PRH/HHEX by Protein Kinase CK2 regulates cell proliferation and cell migration in diverse cell types. In F. Uchiumi (Ed.), Gene expression and regulation in mammalian cells (237--255). InTech. https://doi.org/10.5772/intechopen.72902

Disruption of the regulatory mechanisms that control cell proliferation and cell migration results in multiple disease states including cancer and leukemia. The Proline-Rich Homeodomain protein (PRH)/haematopoietically expressed homeobox protein (HHE... Read More about Phosphorylation of PRH/HHEX by Protein Kinase CK2 regulates cell proliferation and cell migration in diverse cell types.

Immune infiltration in invasive lobular breast cancer (2018)
Journal Article
Desmedt, C., Salgado, R., Fornili, M., Pruneri, G., Gert Van den Eynden, G., Zoppoli, G., …Sotiriou, C. (2018). Immune infiltration in invasive lobular breast cancer. JNCI: Journal of the National Cancer Institute, 110(7), 768–776. https://doi.org/10.1093/jnci/djx268

Background: Invasive lobular breast cancer (ILC) is the second most common histological subtype of breast cancer after invasive ductal cancer (IDC). Here, we aimed at evaluating the prevalence, levels and composition of tumor infiltrating lymphocytes... Read More about Immune infiltration in invasive lobular breast cancer.

Quantitative assessment of the sensitivity of dormant AML cells to the BAD mimetics ABT-199 and ABT-737 (2018)
Journal Article
Yu, N., Seedhouse, C., Russell, N., & Pallis, M. (2018). Quantitative assessment of the sensitivity of dormant AML cells to the BAD mimetics ABT-199 and ABT-737. Leukemia & Lymphoma, 59(10), 2447-2453. https://doi.org/10.1080/10428194.2018.1434884

Cells from patients with acute myeloid leukaemia (AML) that remain dormant and protected by stromal cells may escape effects of chemotherapy. We modelled dormancy in vitro and investigated the ability of Bcl-2 inhibitors ABT-199 and ABT-737 to overco... Read More about Quantitative assessment of the sensitivity of dormant AML cells to the BAD mimetics ABT-199 and ABT-737.

Exposure to the ROCK inhibitor fasudil promotes gliogenesis of neural stem cells in vitro (2018)
Journal Article
Nizamudeen, Z. A., Chakrabarti, L., & Sottile, V. (2018). Exposure to the ROCK inhibitor fasudil promotes gliogenesis of neural stem cells in vitro. Stem Cell Research, 28, 75-86. https://doi.org/10.1016/j.scr.2018.02.001

Fasudil is a clinically approved Rho-associated protein kinase (ROCK) inhibitor that has been used widely to treat cerebral consequences of subarachnoid haemorrhage. It is known to have a positive effect on animal models of neurological disorders inc... Read More about Exposure to the ROCK inhibitor fasudil promotes gliogenesis of neural stem cells in vitro.

The significance of tumour microarchitectural features in breast cancer prognosis: a digital image analysis (2018)
Journal Article
Roxanis, I., Colling, R., Kartsonaki, C., Green, A., & Rakha, E. (2018). The significance of tumour microarchitectural features in breast cancer prognosis: a digital image analysis. Breast Cancer Research, 20(11), https://doi.org/10.1186/s13058-018-0934-x

BACKGROUND: As only a minor portion of the information present in histological sections is accessible by eye, recognition and quantification of complex patterns and relationships among constituents relies on digital image analysis. In this study, our... Read More about The significance of tumour microarchitectural features in breast cancer prognosis: a digital image analysis.

Targeting ataxia telangiectasia mutated and Rad3 related kinase (ATR) in PTEN deficient breast cancers for personalized therapy (2018)
Journal Article
Al-Subhi, N., Ali, R., Abdel-Fatah, T., Moseley, P. M., Chan, S. Y., Green, A. R., …Madhusudan, S. (2018). Targeting ataxia telangiectasia mutated and Rad3 related kinase (ATR) in PTEN deficient breast cancers for personalized therapy. Breast Cancer Research and Treatment, 169(2), 277–286. https://doi.org/10.1007/s10549-018-4683-4

Purpose: PTEN, a negative regulator of PI3K signaling, is involved in DNA repair. ATR is a key sensor of DNA damage and replication stress. We evaluated whether ATR signaling has clinical significance and could be targeted by synthetic lethality in P... Read More about Targeting ataxia telangiectasia mutated and Rad3 related kinase (ATR) in PTEN deficient breast cancers for personalized therapy.

BQ323636.1, a novel splice variant to NCOR2, as a predictor for tamoxifen resistant breast cancer (2018)
Journal Article
Gong, C., Man, E. P., Tsoi, H., Lee, T. K., Paul, L., Mak, S., …Khoo, U. (2018). BQ323636.1, a novel splice variant to NCOR2, as a predictor for tamoxifen resistant breast cancer. Clinical Cancer Research, 24(15), 3681-3691. https://doi.org/10.1158/1078-0432.CCR-17-2259

Purpose: Adjuvant tamoxifen treatment revolutionized the management of estrogen receptor (ER) positive breast cancers to prevent cancer recurrence; however drug resistance compromises its clinical efficacy. The mechanisms underlying tamoxifen resista... Read More about BQ323636.1, a novel splice variant to NCOR2, as a predictor for tamoxifen resistant breast cancer.

Targeting gp100 and TRP-2 with a DNA vaccine: incorporating T cell epitopes with a human IgG1 antibody induces potent T cell responses that are associated with favourable clinical outcome in a phase I/II trial (2018)
Journal Article
Patel, P. M., Ottensmeier, C. H., Mulatero, C., Lorigan, P., Plummer, R., Pandha, H. S., …Durrant, L. (2018). Targeting gp100 and TRP-2 with a DNA vaccine: incorporating T cell epitopes with a human IgG1 antibody induces potent T cell responses that are associated with favourable clinical outcome in a phase I/II trial. OncoImmunology, 7(6), Article e1433516. https://doi.org/10.1080/2162402X.2018.1433516

A DNA vaccine, SCIB1, incorporating two CD8 and two CD4 epitopes from TRP-2/gp100 was evaluated in patients with metastatic melanoma. Each patient received SCIB1 via intramuscular injection with electroporation. The trial was designed to find the saf... Read More about Targeting gp100 and TRP-2 with a DNA vaccine: incorporating T cell epitopes with a human IgG1 antibody induces potent T cell responses that are associated with favourable clinical outcome in a phase I/II trial.

Vascular endothelial growth factor-A165b restores normal glomerular water permeability in a diphtheria-toxin mouse model of glomerular injury (2018)
Journal Article
Stevens, M., Neal, C. R., Salmon, A. H., Bates, D. O., Harper, S. J., & Oltean, S. (in press). Vascular endothelial growth factor-A165b restores normal glomerular water permeability in a diphtheria-toxin mouse model of glomerular injury. Nephron, https://doi.org/10.1159/000485664

BACKGROUND/AIMS:Genetic cell ablation using the human diphtheria toxin receptor (hDTR) is a new strategy used for analysing cellular function. Diphtheria toxin (DT) is a cytotoxic protein that leaves mouse cells relatively unaffected, but upon bindin... Read More about Vascular endothelial growth factor-A165b restores normal glomerular water permeability in a diphtheria-toxin mouse model of glomerular injury.

Morphological alterations of cultured human colorectal matched tumour and healthy organoids (2018)
Journal Article
Mohammad, S., Kashfi, H., Almozyan, S., Jinks, N., Koo, B., & Nateri, A. S. (2018). Morphological alterations of cultured human colorectal matched tumour and healthy organoids. Oncotarget, 9, https://doi.org/10.18632/oncotarget.24279

Organoids have extensive applications in many fields ranging from modelling human development and disease, personalised medicine, drug screening, etc. Moreover, in the last few years, several studies have evaluated the capacity of organoids as transp... Read More about Morphological alterations of cultured human colorectal matched tumour and healthy organoids.

Functional analysis after rapid degradation of condensins and 3D-EM reveals chromatin volume is uncoupled from chromosome architecture in mitosis (2018)
Journal Article
Samejima, K., Booth, D. G., Ogawa, H., Paulson, J. R., Xie, L., Watson, C. A., …Earnshaw, W. C. (2018). Functional analysis after rapid degradation of condensins and 3D-EM reveals chromatin volume is uncoupled from chromosome architecture in mitosis. Journal of Cell Science, 131(4), Article jcs210187. https://doi.org/10.1242/jcs.210187

Effects of hypoxia and hyperoxia on the differential expression of VEGF-A isoforms and receptors in Idiopathic Pulmonary Fibrosis (IPF) (2018)
Journal Article
Barratt, S. L., Blythe, T., Ourradi, K., Jarrett, C., Welsh, G. I., Bates, D. O., & Millar, A. B. (in press). Effects of hypoxia and hyperoxia on the differential expression of VEGF-A isoforms and receptors in Idiopathic Pulmonary Fibrosis (IPF). Respiratory Research, 19, https://doi.org/10.1186/s12931-017-0711-x

Dysregulation of VEGF-A bioavailability has been implicated in the development of lung injury/fibrosis, exemplified by Idiopathic Pulmonary Fibrosis (IPF). VEGF-A is a target of the hypoxic response via its translational regulation by HIF-1?. The rol... Read More about Effects of hypoxia and hyperoxia on the differential expression of VEGF-A isoforms and receptors in Idiopathic Pulmonary Fibrosis (IPF).

Stable DHLA–PEG capped PbS quantum dots: from synthesis to near-infrared biomedical imaging (2018)
Journal Article
Zamberlan, F., Turyanska, L., Patanè, A., Liu, Z., Williams, H., Fay, M., …Grabowska, A. (in press). Stable DHLA–PEG capped PbS quantum dots: from synthesis to near-infrared biomedical imaging. Journal of Materials Chemistry B, 6, https://doi.org/10.1039/c7tb02912h

The short shelf-life of water-soluble quantum dots (QDs) due to colloidal instability represents a major drawback to their exploitation. This work examines the colloidal stability of PbS nanoparticles capped with dihydrolipoic acid–polyethylene glyco... Read More about Stable DHLA–PEG capped PbS quantum dots: from synthesis to near-infrared biomedical imaging.

High nuclear MSK1 is associated with longer survival in breast cancer patients (2018)
Journal Article
Pu, X., Storr, S. J., Ahmad, N., Rakha, E., Green, A. R., Ellis, I. O., & Martin, S. G. (2018). High nuclear MSK1 is associated with longer survival in breast cancer patients. Journal of Cancer Research and Clinical Oncology, 144(3), https://doi.org/10.1007/s00432-018-2579-7

Purpose: Mitogen- and stress- activated kinases (MSKs) are important substrates of the mitogen-activated protein kinase (MAPK)-activated protein kinase family. MSK1 and MSK2 are both nuclear serine/threonine protein kinases, with MSK1 being suggested... Read More about High nuclear MSK1 is associated with longer survival in breast cancer patients.