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The clinical and biological significance of HER2 over-expression in breast ductal carcinoma in situ: a large study from a single institution (2019)
Journal Article
Miligy, I. M., Toss, M. S., Gorringe, K. L., Lee, A. H., Ellis, I. O., Green, A. R., & Rakha, E. (2019). The clinical and biological significance of HER2 over-expression in breast ductal carcinoma in situ: a large study from a single institution. British Journal of Cancer, doi:10.1038/s41416-019-0436-3

Background: Previous studies have reported up to 50% of ductal carcinoma in situ (DCIS), is HER2 positive, but the frequency of HER2 positive invasive breast cancer (IBC) is lower. The aim of this study is to characterise HER2 status in DCIS and asse... Read More

A Key Genomic Subtype Associated with Lymphovascular Invasion in Invasive Breast Cancer (2019)
Journal Article
Kurozumi, S., Joseph, C., Alsaeed, S., Kariri, Y., Aljohani, A., Raafat, S., …Rakha, E. A. (in press). A Key Genomic Subtype Associated with Lymphovascular Invasion in Invasive Breast Cancer. British Journal of Cancer,

Background: Lymphovascular invasion (LVI) is associated with the development of metastasis in invasive breast cancer (BC). However, the complex molecular mechanisms of LVI, which overlap with other oncogenic pathways, remain unclear. This study, usin... Read More

Adaptive Changes of Glioblastoma Cells Following Exposure to Hypoxic (1% Oxygen) Tumour Microenvironment (2019)
Journal Article
Musah-Eroje, A., & Watson, S. (2019). Adaptive Changes of Glioblastoma Cells Following Exposure to Hypoxic (1% Oxygen) Tumour Microenvironment. International Journal of Molecular Sciences, 20(9), 1-16. doi:10.3390/ijms20092091

Glioblastoma multiforme is the most aggressive and malignant primary brain tumour, with a median survival rate of between 15 to 17 months. Heterogeneous regions occur in glioblastoma as a result of oxygen gradients which ranges from 0.1% to 10% in vi... Read More

Porous phosphate-based glass microspheres show biocompatibility, tissue infiltration and osteogenic onset in an ovine bone defect model (2019)
Journal Article
McLaren, J. S., Macri-Pellizzeri, L., Zakir Hossain, K. M., Patel, U., Grant, D. M., Scammell, B. E., …Sottile, V. (2019). Porous phosphate-based glass microspheres show biocompatibility, tissue infiltration and osteogenic onset in an ovine bone defect model. ACS Applied Materials and Interfaces, 11(17), 15436–15446. doi:10.1021/acsami.9b04603

Phosphate-based glasses (PBG) are bioactive and fully degradable materials with tailorable degradation rates. PBGs can be produced as porous microspheres through a single-step process, using changes in their formulation and geometry to produce varyin... Read More

Impaired circulating myeloid CD1c+ dendritic cell function in human glioblastoma is restored by p38 inhibition - implications for the next generation of DC vaccines (2019)
Journal Article
Adhikaree, J., Franks, H. A., Televantos, C., Vaghela, P., Kaur, A. P., Walker, D., …Patel, P. M. (2019). Impaired circulating myeloid CD1c+ dendritic cell function in human glioblastoma is restored by p38 inhibition - implications for the next generation of DC vaccines. OncoImmunology, 8(7), doi:10.1080/2162402X.2019.1593803

Current treatments for glioblastoma (GBM) have limited efficacy and significant morbidity and therefore new strategies are urgently needed. Dendritic cells have the power to create anti-tumour immune responses. The greater potency of circulating den... Read More

Penetration and uptake of Nanoparticles in 3D tumour spheroids (2019)
Journal Article
Tchoryk, A., Taresco, V., Argent, R., Ashford, M. B., Gellert, P., Stolnik, S., …Garnett, M. C. (2019). Penetration and uptake of Nanoparticles in 3D tumour spheroids. Bioconjugate Chemistry, doi:10.1021/acs.bioconjchem.9b00136

Animal models are effective for assessing tumour localisation of nanosystems, but difficult to use for studying penetration beyond the vasculature. Here, we have used well-characterised HCT116 colorectal cancer spheroids to study the effect of nanopa... Read More

Magnetically Assisted Control of Stem Cells Applied in 2D, 3D and In Situ Models of Cell Migration (2019)
Journal Article
Harrison, R., Luckett, J., Marsh, S., Lugo Leija, H. A., Salih, S., Alkharji, R., & Sottile, V. (2019). Magnetically Assisted Control of Stem Cells Applied in 2D, 3D and In Situ Models of Cell Migration. Molecules, 24(8), doi:10.3390/molecules24081563

The success of cell therapy approaches is greatly dependent on the ability to precisely deliver and monitor transplanted stem cell grafts at treated sites. Iron oxide particles, traditionally used in vivo for magnetic resonance imaging (MRI), have be... Read More

Signaling networks in cholangiocarcinoma: molecular pathogenesis, targeted therapies and drug resistance (2019)
Journal Article
Fouassier, L., Marzioni, M., Afonso, M. B., Dooley, S., Gaston, K., Giannelli, G., …Coulouarn, C. (2019). Signaling networks in cholangiocarcinoma: molecular pathogenesis, targeted therapies and drug resistance. Liver International, doi:10.1111/liv.14102

Cholangiocarcinoma (CCA) is a deadly disease. While surgery may attain cure in a minor fraction of cases, therapeutic options in either the adjuvant or advanced setting are limited. The possibility of advancing the efficacy of therapeutic approaches... Read More

Exploring the experiences of cancer patients with chemotherapy induced ototoxicity: qualitative study using online health forums (2019)
Journal Article
Pearson, S. E., Taylor, J. A., Hoare, D. J., Patel, P., & Baguley, D. M. (2019). Exploring the experiences of cancer patients with chemotherapy induced ototoxicity: qualitative study using online health forums. JMIR Cancer, 5(1), doi:10.2196/10883

Background: Many cancer patients and survivors experience permanent and life-debilitating effects, such as ototoxicity, from treatment. Ototoxicity manifests as high-frequency hearing loss and tinnitus, which can have a detrimental effect on the qual... Read More

Dynamics of breast-cancer relapse reveal late-recurring ER-positive genomic subgroups (2019)
Journal Article
Rueda, O. M., Sammut, S., Seoane, J. A., Chin, S., Caswell-Jin, J. L., Callari, M., …Curtis, C. (2019). Dynamics of breast-cancer relapse reveal late-recurring ER-positive genomic subgroups. Nature, 567, 399–404. doi:10.1038/s41586-019-1007-8

The rates and routes of lethal systemic spread in breast cancer are poorly understood owing to a lack of molecularly characterized patient cohorts with long-term, detailed follow-up data. Long-term follow-up is especially important for those with oes... Read More

Atypical ductal hyperplasia is a multipotent precursor of breast carcinoma (2019)
Journal Article
Kader, T., Hill, P., Zethoven, M., Goode, D. L., Elder, K., Thio, N., …Gorringe, K. L. (2019). Atypical ductal hyperplasia is a multipotent precursor of breast carcinoma. Journal of Pathology, doi:10.1002/path.5262

The current model for breast cancer progression proposes independent “low‐grade (LG) like” and “high‐grade (HG) like” pathways but lacks a known precursor to HG cancer. We applied low coverage whole genome sequencing to atypical ductal hyperplasia (A... Read More

The genetic vulnerability to cisplatin ototoxicity: a systematic review (2019)
Journal Article
Tserga, E., Nandwani, T., Edvall, N. K., Bulla, J., Patel, P., Canlon, B., …Baguley, D. M. (2019). The genetic vulnerability to cisplatin ototoxicity: a systematic review. Scientific Reports, 9, doi:10.1038/s41598-019-40138-z

Ototoxicity is one of the major side-effects of platinum-based chemotherapy, in particular cisplatin (cis-diammine dichloroplatinum II). To our knowledge, no systematic review has previously provided a quantitative summary estimate of the impact of g... Read More

SHON expression predicts response and relapse risk of breast cancer patients after anthracycline-based combination chemotherapy or tamoxifen treatment (2019)
Journal Article
Abdel-Fatah, T. M., Broom, R. J., Lu, J., Moseley, P. M., Huang, B., Li, L., …Liu, D. (2019). SHON expression predicts response and relapse risk of breast cancer patients after anthracycline-based combination chemotherapy or tamoxifen treatment. British Journal of Cancer, 120, 728–745. doi:10.1038/s41416-019-0405-x

BACKGROUND: SHON nuclear expression (SHON-Nuc+) was previously reported to predict clinical outcomes to tamoxifen therapy in ERα+ breast cancer (BC). Herein we determined if SHON expression detected by specific monoclonal antibodies could provide a m... Read More

T cell repertoire to citrullinated self-peptides in healthy humans is not confined to the HLA-DR SE alleles; targeting of citrullinated self-peptides presented by HLA-DP4 for tumour therapy (2019)
Journal Article
Brentville, V., Symonds, P., Cook, K. W., Daniels, I., Pitt, T., Gijon, M., …Durrant, L. G. (2019). T cell repertoire to citrullinated self-peptides in healthy humans is not confined to the HLA-DR SE alleles; targeting of citrullinated self-peptides presented by HLA-DP4 for tumour therapy. OncoImmunology, 8(5), doi:10.1080/2162402X.2019.1576490

Post-translational modifications are induced in stressed cells which cause them to be recognised by the immune system. One such modification is citrullination where the positive charged arginine is modified to a neutral citrulline. We demonstrate mos... Read More

An FBXW7-ZEB2 axis links EMT and tumour microenvironment to promote colorectal cancer stem cells and chemoresistance (2019)
Journal Article
Li, N., Babaei-Jadidi, R., Lorenzi, F., Spencer-Dene, B., Clarke, P., Domingo, E., …Nateri, A. S. (2019). An FBXW7-ZEB2 axis links EMT and tumour microenvironment to promote colorectal cancer stem cells and chemoresistance. Oncogenesis, 8, doi:10.1038/s41389-019-0125-3

Colorectal cancer (CRC) patients develop recurrence after chemotherapy owing to the survival of stem cell-like cells referred to as cancer stem-like cells (CSCs). The origin of CSCs is linked to the epithelial–mesenchymal transition (EMT) process. Cu... Read More

Clinicopathological significance of ataxia-telangiectasia-mutated (ATM) kinase and ataxia telangiectasia-mutated and Rad3 related (ATR) kinase in MYC overexpressed breast cancers (2019)
Journal Article
Savva, C., De Souza, K., Ali, R., Rakha, E. A., Green, A. R., & Madhusudan, S. (2019). Clinicopathological significance of ataxia-telangiectasia-mutated (ATM) kinase and ataxia telangiectasia-mutated and Rad3 related (ATR) kinase in MYC overexpressed breast cancers. Breast Cancer Research and Treatment, 175(1), 105–115. doi:10.1007/s10549-018-05113-8

Purpose: MYC transcription factor has critical roles in cell growth, proliferation, metabolism, differentiation, transformation and angiogenesis. MYC overexpression is seen in about 15% of breast cancers and linked to aggressive phenotypes. MYC overe... Read More

Expression of Syk and MAP4 proteins in ovarian cancer (2019)
Journal Article
Zhang, S., Deen, S., Storr, S. J., Yao, A., & Martin, S. G. (2019). Expression of Syk and MAP4 proteins in ovarian cancer. Journal of Cancer Research and Clinical Oncology, 145(4), 909–919. doi:10.1007/s00432-019-02856-9

Purpose We have previously reported on the prognostic importance of the calpain family of proteins in ovarian cancer, especially calpain-2. Spleen tyrosine kinase (Syk) phosphorylates a variety of cytoskeletal proteins with studies suggesting potent... Read More

A novel 3D in vitro model of glioblastoma reveals resistance to temozolomide which was potentiated by hypoxia (2019)
Journal Article
Musah-Eroje, A., & Watson, S. (2019). A novel 3D in vitro model of glioblastoma reveals resistance to temozolomide which was potentiated by hypoxia. Journal of Neuro-Oncology, 142(2), 231-240. doi:10.1007/s11060-019-03107-0

Purpose Glioblastoma (GBM) is the most common invasive malignant brain tumour in adults. It is traditionally investigated in vitro by culturing cells as a monolayer (2D culture) or as neurospheres (clusters enriched in cancer stem cells) but neither... Read More

Liver kinase B1—a potential therapeutic target in hormone-sensitive breast cancer in older women (2019)
Journal Article
Syed, B. M., Green, A. R., Morgan, D. A., Ellis, I. O., & Cheung, K. (2019). Liver kinase B1—a potential therapeutic target in hormone-sensitive breast cancer in older women. Cancers, 11(2), 1-17. doi:10.3390/cancers11020149

Background: The role of liver kinase B1 (LKB1), a serine/threonine kinase, has been described in the development of PeutzJagher’s syndrome, where a proportion (~45%) of patients have developed breast cancer in their lifetime. Cell line studies have l... Read More

Liver kinase B1 (LKB1) – a potential therapeutic target in hormone sensitive breast cancer in older women (2019)
Journal Article
Syed, B. M., Green, A. R., Morgan, D. A., Ellis, I. O., & Cheung, K. (2019). Liver kinase B1 (LKB1) – a potential therapeutic target in hormone sensitive breast cancer in older women. Cancers, 11(2), 1-17

Background: The role of liver kinase B1 (LKB1), a serine/threonine kinase, has been described in the development of PeutzJagher’s syndrome, where a proportion (~45%) of patients have developed breast cancer in their lifetime. Cell line studies have l... Read More