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Prognostic Significance of Nucleolar Assessment in Invasive Breast Cancer (2019)
Journal Article
Elsharawy, K. A., Toss, M. S., Raaft, S., Ball, G., Green, A. R., Aleskandarany, M. A., …Rakha, E. (in press). Prognostic Significance of Nucleolar Assessment in Invasive Breast Cancer. Histopathology,

Aims: Nucleolar morphometric features have a potential role in the assessment of aggressiveness of many cancers. However, the role of nucleoli in invasive breast cancer (IBC) is still unclear. This study aimed to investigate the optimal scoring metho... Read More

Clinicopathological significance of lipocalin 2 nuclear expression in invasive breast cancer (2019)
Journal Article
Kurozumi, S., Alsaeed, S., Orah, N., Miligy, I. M., Joseph, C., Aljohani, A., …Rakha, E. A. (2019). Clinicopathological significance of lipocalin 2 nuclear expression in invasive breast cancer. Breast Cancer Research and Treatment, https://doi.org/10.1007/s10549-019-05488-2

PURPOSE: The epithelial–mesenchymal transition (EMT) plays a key role in breast cancer progression and metastasis. Lipocalin 2 (LCN2) is involved in the regulation of EMT. The aim of this study was to investigate the clinicopathological significance... Read More

Prognostic Significance of KN Motif and Ankyrin Repeat Domains 1 (KANK1) in Invasive Breast Cancer (2019)
Journal Article
Kariri, Y. A., Joseph, C., Kurozumi, S., Toss, M. S., Alsaleem, M., Raafat, S., …Rakha, E. A. (2019). Prognostic Significance of KN Motif and Ankyrin Repeat Domains 1 (KANK1) in Invasive Breast Cancer. Breast Cancer Research and Treatment, https://doi.org/10.1007/s10549-019-05466-8

Background: KN Motif and Ankyrin Repeat Domains 1 (KANK1) plays an important role in cytoskeleton maintenance and contributes to the regulation of cell proliferation, adhesion and apoptosis. KANK1 is involved in progression of a variety of solid tumo... Read More

High-Throughput Phenotyping Toolkit for Characterizing Cellular Models of Hypertrophic Cardiomyopathy in Vitro (2019)
Journal Article
Mosqueira, D., Lis-Slimak, K., & Denning, C. (2019). High-Throughput Phenotyping Toolkit for Characterizing Cellular Models of Hypertrophic Cardiomyopathy in Vitro. Methods and Protocols, 2(4), 1-26. https://doi.org/10.3390/mps2040083

Hypertrophic cardiomyopathy (HCM) is a prevalent and complex cardiovascular disease characterised by multifarious hallmarks, a heterogeneous set of clinical manifestations, and several molecular mechanisms. Various disease models have been developed... Read More

Numerical Simulation of a Scanning Illumination System for Deep Tissue Fluorescence Imaging (2019)
Journal Article
Zhang, Q., Grabowska, A. M., Clarke, P. A., & Morgan, S. P. (2019). Numerical Simulation of a Scanning Illumination System for Deep Tissue Fluorescence Imaging. Journal of Imaging, 5(11), https://doi.org/10.3390/jimaging5110083

he spatial resolution and light detected in fluorescence imaging for small animals are limited by light scattering, absorption and autofluorescence. To address this, novel near-infrared fluorescent contrast agents and imaging configurations have been... Read More

ATM Regulated PTEN Degradation Is XIAP E3 Ubiquitin Ligase Mediated in p85α Deficient Cancer Cells and Influence Platinum Sensitivity (2019)
Journal Article
Ali, R., Alabdullah, M., Miligy, I., Normatova, M., Babaei-Jadidi, R., S. Nateri, A., …Madhusudan, S. (2019). ATM Regulated PTEN Degradation Is XIAP E3 Ubiquitin Ligase Mediated in p85α Deficient Cancer Cells and Influence Platinum Sensitivity. Cells, 8(10), https://doi.org/10.3390/cells8101271

Ataxia-telegiectasia mutated (ATM), phosphatase and tensin homolog (PTEN), and p85α are key tumour suppressors. Whether ATM regulates PTEN expression and influence platinum sensitivity is unknown. We generated ATM knockdowns (KD) and CRISPR knock out... Read More

Impact of Serum Source on Human Mesenchymal Stem Cell Osteogenic Differentiation in Culture (2019)
Journal Article
Popov, A., Scotchford, C., Grant, D., & Sottile, V. (2019). Impact of Serum Source on Human Mesenchymal Stem Cell Osteogenic Differentiation in Culture. International Journal of Molecular Sciences, 20(20), https://doi.org/10.3390/ijms20205051

Human mesenchymal stem cells (MSCs) show promise for musculoskeletal repair applications. Animal-derived serum is extensively used for MSC culture as a source of nutrients, extracellular matrix proteins and growth factors. However, the routine use of... Read More

The prognostic significance of wild type isocitrate dehydrogenase 2 (IDH2) in breast cancer (2019)
Journal Article
Aljohani, A. I., Toss, M. S., Kurozumi, S., Joseph, C., Aleskandarany, M. A., Miligy, I. M., …Rakha, E. (2019). The prognostic significance of wild type isocitrate dehydrogenase 2 (IDH2) in breast cancer. Breast Cancer Research and Treatment, https://doi.org/10.1007/s10549-019-05459-7

Background: Lymphovascular invasion (LVI) is a prerequisite step in breast cancer (BC) metastasis. We have previously identified wild type isocitrate dehydrogenase 2 (IDH2) as a key putative driver of LVI. Thus, we explored the prognostic significanc... Read More

The Role Of Glutaminase In Cancer (2019)
Journal Article
Masisi, B., El Ansari, R., Alfarsi, L., Rakha, E., Green, A., & Craze, M. (2019). The Role Of Glutaminase In Cancer. Histopathology, https://doi.org/10.1111/his.14014

Increased glutamine metabolism (glutaminolysis) is a hallmark of cancer and is recognised as a key metabolic change in cancer cells. As a heterogeneous disease with different morpholog- ical and molecular subtypes and response to therapy, breast canc... Read More

Determining responsiveness and meaningful changes for the Musculoskeletal Health Questionnaire (MSK-HQ) for use across musculoskeletal care pathways (2019)
Journal Article
Price, A. J., Ogollah, R., Kang, S., Hay, E., Barker, K. L., Benedetto, E., …Hill, J. C. (2019). Determining responsiveness and meaningful changes for the Musculoskeletal Health Questionnaire (MSK-HQ) for use across musculoskeletal care pathways. BMJ Open, 9(10), 1-7. https://doi.org/10.1136/bmjopen-2018-025357

Objectives: We have previously developed and validated the Arthritis Research UK Musculoskeletal Health Questionnaire (MSK-HQ) for use across musculoskeletal care pathways, showing encouraging psychometric test results. The objective of this study wa... Read More

Cancer survivors treated with platinum-based chemotherapy affected by ototoxicity and the impact on quality of life: a narrative synthesis systematic review (2019)
Journal Article
Pearson, S. E., Taylor, J., Patel, P., & Baguley, D. M. (2019). Cancer survivors treated with platinum-based chemotherapy affected by ototoxicity and the impact on quality of life: a narrative synthesis systematic review. International Journal of Audiology, 58(11), 685-695. https://doi.org/10.1080/14992027.2019.1660918

Lipid species affect morphology of endoplasmic reticulum: a sea urchin oocyte model of reversible manipulation (2019)
Journal Article
Ulloa, G., Hamati, F., Dick, A., Fitzgerald, J., Mantell, J., Verkade, P., …Poccia, D. (2019). Lipid species affect morphology of endoplasmic reticulum: a sea urchin oocyte model of reversible manipulation. Journal of Lipid Research, https://doi.org/10.1194/jlr.ra119000210

The endoplasmic reticulum (ER) is a large multifunctional organelle of eukaryotic cells. Malfunction of the ER in various disease states, such as atherosclerosis, Type 2 diabetes, cancer, Alzheimer’s and Parkinson’s diseases and amyotrophic lateral s... Read More

Simultaneous measurement of excitation-contraction coupling parameters identifies mechanisms underlying contractile responses of hiPSC-derived cardiomyocytes (2019)
Journal Article
Van Meer, B. J., Krotenberg, A., Sala, L., Davis, R. P., Eschenhagen, T., Denning, C., …Mummery, C. (2019). Simultaneous measurement of excitation-contraction coupling parameters identifies mechanisms underlying contractile responses of hiPSC-derived cardiomyocytes. Nature Communications, 10, https://doi.org/10.1038/s41467-019-12354-8

Cardiomyocytes from human induced pluripotent stem cells (hiPSC-CMs) are increasingly recognized as valuable for determining the effects of drugs on ion channels but they do not always accurately predict contractile responses of the human heart. This... Read More

The intra-tumoral stroma in patients with breast cancer increases with age (2019)
Journal Article
Vangangelt, K., Kramer, C., Bastiaannet, E., Putter, H., Cohen, D., Van Pelt, G., …Mesker, W. (2019). The intra-tumoral stroma in patients with breast cancer increases with age. Breast Cancer Research and Treatment, https://doi.org/10.1007/s10549-019-05422-6

Purpose The tumor microenvironment in older patients is subject to changes. The tumor-stroma ratio (TSR) was evaluated in order to estimate the amount of intra-tumoral stroma and to evaluate the prognostic value of the TSR in older patients with br... Read More

Validation and assessment of an antibiotic-based, aseptic decontamination manufacturing protocol for therapeutic, vacuum-dried human amniotic membrane (2019)
Journal Article
Marsit, N. M., Sidney, L. E., Britchford, E. R., McIntosh, O. D., Allen, C. L., Ashraf, W., …Hopkinson, A. (2019). Validation and assessment of an antibiotic-based, aseptic decontamination manufacturing protocol for therapeutic, vacuum-dried human amniotic membrane. Scientific Reports, 9(1), https://doi.org/10.1038/s41598-019-49314-7

Activated zinc transporter ZIP7 as an indicator of anti-hormone resistance in breast cancer (2019)
Journal Article
Zilio, S., Gee, J. M. W., Ellis, I. O., Green, A. R., Finlay, P., Gobbato, A., & Taylor, K. M. (2019). Activated zinc transporter ZIP7 as an indicator of anti-hormone resistance in breast cancer. Metallomics, https://doi.org/10.1039/C9MT00136K

ZIP7, a member of the ZIP family of zinc importers, resides on the endoplasmic reticulum membrane and transports zinc from intracellular stores to the cytoplasm after activation by CK2 phosphorylation on two serine residues (S275 and S276). ZIP7 is k... Read More

The prognostic significance of Cathepsin V (CTSV/CTSL2) in breast ductal carcinoma in situ (2019)
Journal Article
Toss, M. S., Miligy, I. M., Gorringe, K. L., Mittal, K., Green, A. R., Aneja, R., …Rakha, E. A. (2019). The prognostic significance of Cathepsin V (CTSV/CTSL2) in breast ductal carcinoma in situ. Journal of Clinical Pathology, doi:10.1136/jclinpath-2019-205939

Aims: Cathepsin V (CTSV/CTSL2) is a lysosomal cystine proteinase and plays a role in extracellular matrix degradation. It is associated with poor prognosis in invasive breast cancer (IBC), but its role in breast ductal carcinoma in situ (DCIS) remain... Read More