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MUSCLEMOTION: a versatile open software tool to quantify cardiomyocyte and cardiac muscle contraction in vitro and in vivo (2017)
Journal Article
Sala, L., van Meer, B., Tertoolen, L., Bakkers, J., Bellin, M., Davis, R., …Burton, F. (2018). MUSCLEMOTION: a versatile open software tool to quantify cardiomyocyte and cardiac muscle contraction in vitro and in vivo. Circulation Research, 122(3), e5. doi:10.1161/CIRCRESAHA.117.312067

Rationale: There are several methods to measure cardiomyocyte (CM) and muscle contraction but these require customized hardware, expensive apparatus and advanced informatics or can only be used in single experimental models. Consequently, data and te... Read More

Model-based image analysis of a tethered Brownian fibre for shear stress sensing (2017)
Journal Article
Gallagher, M. T., Neal, C. V., Arkill, K. P., & Smith, D. J. (2017). Model-based image analysis of a tethered Brownian fibre for shear stress sensing. Interface, 14(137), doi:10.1098/rsif.2017.0564

The measurement of fluid dynamic shear stress acting on a biologically relevant surface is a challenging problem, particularly in the complex environment of, for example, the vasculature. While an experimental method for the direct detection of wall... Read More

A molecular signature of dormancy in CD34+CD38- acute myeloid leukaemia cells (2017)
Journal Article
Al-Asadi, M. G., Brindle, G., Castellanos, M., May, S., Mills, K. I., Russell, N., …Pallis, M. (2017). A molecular signature of dormancy in CD34+CD38- acute myeloid leukaemia cells. Oncotarget, 8(67), 111405-111418. doi:10.18632/oncotarget.22808

Dormant leukaemia initiating cells in the bone marrow niche are a crucial therapeutic target for total eradication of acute myeloid leukaemia. To study this cellular subset we created and validated an in vitro model employing the cell line TF-1a, tre... Read More

MYC regulation of Glutamine-Proline regulatory axis is key in Luminal B breast cancer (2017)
Journal Article
Craze, M. L., Cheung, H., Jewa, N., Coimbra, N. D., Soria, D., El-Ansari, R., …Green, A. R. (in press). MYC regulation of Glutamine-Proline regulatory axis is key in Luminal B breast cancer. British Journal of Cancer, doi:10.1038/bjc.2017.387

Background: Altered cellular metabolism is a hallmark of cancer and some are reliant on Glutamine for sustained proliferation and survival. We hypothesise that the Glutamine-Proline regulatory axis has a key role in Breast cancer (BC) in the highly p... Read More

Regulation of human feto-placental endothelial barrier integrity by vascular endothelial growth factors: competitive interplay between VEGF-A165a, VEGF-A165b, PIGF and VE-cadherin (2017)
Journal Article
Pang, V., Bates, D. O., & Leach, L. (2017). Regulation of human feto-placental endothelial barrier integrity by vascular endothelial growth factors: competitive interplay between VEGF-A165a, VEGF-A165b, PIGF and VE-cadherin. Clinical Science, 131(23), 2763-2775. doi:10.1042/CS20171252

The human placenta nourishes and protects the developing fetus whilst influencing maternal physiology for fetal advantage. It expresses several members of the VEGF family including the pro-angiogenic/pro-permeability VEGF-A165a isoform, the anti-angi... Read More

Evaluation of CDK12 protein expression as a potential novel biomarker for DNA damage response–targeted therapies in breast cancer (2017)
Journal Article
Naidoo, K., Wai, P. T., Maguire, S. L., Daley, F., Haider, S., Kriplani, D., …Natrajan, R. (2018). Evaluation of CDK12 protein expression as a potential novel biomarker for DNA damage response–targeted therapies in breast cancer. Molecular Cancer Therapeutics, 17(1), 306-315. doi:10.1158/1535-7163.mct-17-0760

Disruption of Cyclin-Dependent Kinase 12 (CDK12) is known to lead to defects in DNA repair and sensitivity to platinum salts and PARP1/2 inhibitors. However, CDK12 has also been proposed as an oncogene in breast cancer. We therefore aimed to assess t... Read More

Citrullinated α-enolase is an effective target for anti-cancer immunity (2017)
Journal Article
Cook, K., Daniels, I., Symonds, P., Pitt, T., Gijon, M., Xue, W., …Brentville, V. A. (2018). Citrullinated α-enolase is an effective target for anti-cancer immunity. OncoImmunology, 7(2), doi:10.1080/2162402X.2017.1390642

Targeting post-translationally modified epitopes may provide a new strategy for generating tumor specific immune responses. Citrullination is the post-translational modification of arginine to citrulline catalyzed by peptidylarginine deaminase (PAD)... Read More

Comparative aspects of canine and human inflammatory breast cancer (2017)
Journal Article
Raposo, T., Arias-Pulido, H., Chaher, N., Fiering, S. N., Argyle, D. J., Prada, J., …Queiroga, F. L. (in press). Comparative aspects of canine and human inflammatory breast cancer. Seminars in Oncology, 44(4), doi:10.1053/j.seminoncol.2017.10.012

Inflammatory breast cancer (IBC) in humans is the most aggressive form of mammary gland cancer and shares clinical, pathologic, and molecular patterns of disease with canine inflammatory mammary carcinoma (CIMC). Despite the use of multimodal therape... Read More

In vitro tissue microarrays for quick and efficient spheroid characterisation (2017)
Journal Article
Ivanov, D. P., & Grabowska, A. M. (2018). In vitro tissue microarrays for quick and efficient spheroid characterisation. Slas Discovery, 23(2), doi:10.1177/2472555217740576

Three-dimensional in vitro microphysiological cultures, such as spheroids and organoids, promise increased patient relevance and therapeutic predictivity compared to reductionist cell monolayers. However, high-throughput characterisation techniques f... Read More

Drug-mediated shortening of action potentials in LQTS2 hiPSC-cardiomyocytes (2017)
Journal Article
Duncan, G., Firth, K. S., George, V., Hoang, M. D., Staniforth, A., Smith, G., & Denning, C. (2017). Drug-mediated shortening of action potentials in LQTS2 hiPSC-cardiomyocytes. Stem Cells and Development, 26(23), doi:10.1089/scd.2017.0172

Cardiomyocytes (CMs) derived from human induced pluripotent stem cells (hiPSCs) are now a well-established modality for modeling genetic disorders of the heart. This is especially so for long QT syndrome (LQTS), which is caused by perturbation of ion... Read More

Reply to Rosen (2017)
Journal Article
Reis-Filho, J. S., Geyer, F. C., Weigelt, B., Rakha, E. A., Ellis, I. O., & Schnitt, S. J. (2017). Reply to Rosen. Modern Pathology, 30(10), 1505-1506. doi:10.1038/modpathol.2017.70

Control of aggregation temperatures in mixed and blended cytocompatible thermoresponsive block co-polymer nanoparticles (2017)
Journal Article
Foralosso, R., Moir, L., Mastrotto, F., Sasso, L., Tchoryk, A., Selo, A., …Alexander, C. (2017). Control of aggregation temperatures in mixed and blended cytocompatible thermoresponsive block co-polymer nanoparticles. Soft Matter, 40(13), doi:10.1039/c7sm00920h

A small library of thermoresponsive amphiphilic copolymers based on polylactide-block-poly((2-(2-methoxyethoxy)ethyl methacrylate)-co-(oligoethylene glycol methacrylate)) (PLA-b-P(DEGMA)-co-(OEGMA)), was synthesised by copper-mediated controlled radi... Read More

Sulfated galactans from the red seaweed Gracilaria fisheri exerts anti-migration effect on cholangiocarcinoma cells (2017)
Journal Article
Sae-Lao, T., Luplertlop, N., Janvilisri, T., Tohtong, R., Bates, D. O., & Wongprasert, K. (2017). Sulfated galactans from the red seaweed Gracilaria fisheri exerts anti-migration effect on cholangiocarcinoma cells. Phytomedicine, 36, doi:10.1016/j.phymed.2017.09.014

BACKGROUND: Seaweeds have a long history of use in Asian countries as functional foods, medicinal herbs, and the treatment of cancer. Polysaccharides from various seaweeds have shown anti-tumor activity. Cholangiocarcinoma (CCA), often with metastati... Read More

Cten promotes epithelial-mesenchymal transition through post-transcriptional stabilization of Snail (2017)
Journal Article
Thorpe, H., Asiri, A., Akhlaq, M., & Ilyas, M. (in press). Cten promotes epithelial-mesenchymal transition through post-transcriptional stabilization of Snail. Molecular Carcinogenesis, 56(12), doi:10.1002/mc.22704

Cten promotes cell migration however the knowledge of underlying signalling pathways is sparse. We have shown that Cten downregulates E-cadherin, a feature of epithelial to mesenchymal transition (EMT). This prompted us to investigate whether Cten fu... Read More

Protein kinase CK2 inhibition suppresses neointima formation via a proline-rich homeodomain-dependent mechanism (2017)
Journal Article
Wadey, K., Brown, B., Sala-Newby, G., Jayaraman, P., Gaston, K., & George, S. (2017). Protein kinase CK2 inhibition suppresses neointima formation via a proline-rich homeodomain-dependent mechanism. Vascular Pharmacology, 99, (34--44). doi:10.1016/j.vph.2017.09.004. ISSN 1537-1891

Neointimal hyperplasia is a product of VSMC replication and consequent accumulation within the blood vessel wall. In this study, we determined whether inhibition of protein kinase CK2 and the resultant stabilisation of proline-rich homeodomain (PRH)... Read More

Breast cancer induced nociceptor aberrant growth and collateral sensory axonal branching (2017)
Journal Article
Austin, M., Elliott, L., Nicolaou, N., Grabowska, A. M., & Hulse, R. P. (2017). Breast cancer induced nociceptor aberrant growth and collateral sensory axonal branching. Oncotarget, 8(44), doi:10.18632/oncotarget.20609

The tumour and neuron interaction has a significant impact upon disease progression and the patients quality of life. In breast cancer patients, it is known that there is an interaction between the tumour microenvironment and the sensory neurons to i... Read More

High mobility group protein B1 is a predictor of poor survival in ovarian cancer (2017)
Journal Article
Machado, L. R., Moseley, P. M., Moss, R., Deen, S., Nolan, C. C., Spendlove, I., …Durrant, L. (2017). High mobility group protein B1 is a predictor of poor survival in ovarian cancer. Oncotarget, doi:10.18632/oncotarget.20538

High-mobility group protein B1 (HMGB1) has been implicated in numerous tumour types where expression regulates tumour cell growth and survival. We hypothesised that high HMGB1 expression in ovarian tumours would predict poor patient survival. Using... Read More

The enigmatic origin of papillomavirus protein domains (2017)
Journal Article
Puustusmaa, M., Kirsip, H., Gaston, K., & Abroi, A. (2017). The enigmatic origin of papillomavirus protein domains. Viruses, 9(9), doi:10.3390/v9090240

Almost a century has passed since the discovery of papillomaviruses. A few decades of research have given a wealth of information on the molecular biology of papillomaviruses. Several excellent studies have been performed looking at the long- and sho... Read More

Rho-GTPase activating protein 18: a biomarker associated with good prognosis in invasive breast cancer (2017)
Journal Article
Aleskandarany, M. A., Sonbul, S. N., Surridge, R., Mukherjee, A., Caldas, C., Diez-Rodriguez, M., …Rakha, E. A. (in press). Rho-GTPase activating protein 18: a biomarker associated with good prognosis in invasive breast cancer. British Journal of Cancer, 117, doi:10.1038/bjc.2017.261

Background: The prognostic value of lymphovascular invasion (LVI) in breast cancer (BC) has been demonstrated in several independent studies. However, identification of driver molecules for LVI remains a challenging task. Large-scale transcriptomic p... Read More