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Molecular classification of breast cancer: what the pathologist needs to know (2016)
Journal Article
Rakha, E. A., & Green, A. R. (2017). Molecular classification of breast cancer: what the pathologist needs to know. Pathology, 49(2), 111-119. doi:10.1016/j.pathol.2016.10.012

Breast cancer is a heterogeneous disease featuring distinct histological, molecular and clinical phenotypes. Although traditional classification systems utilising clinicopathological and few molecular markers are well established and validated, they... Read More

Prognostic stratification of oestrogen receptor-positive HER2-negative lymph node-negative class of breast cancer (2016)
Journal Article
Rakha, E. A., Agarwal, D., Green, A. R., Ashankyty, I., Ellis, I. O., Ball, G., & Alaskandarany, M. A. (2017). Prognostic stratification of oestrogen receptor-positive HER2-negative lymph node-negative class of breast cancer. Histopathology, 70(4), 622-631. doi:10.1111/his.13108

Aims Multigene assay is recommended currently for prognostic stratification of the clinically indeterminate group of breast cancer (BC) patients defined as lymph node (LN)‐negative, oestrogen receptor (ER)‐positive, HER2‐negative (LN−/ER+/HER2−) t... Read More

Diagnostic concordance of breast pathologists: lessons from the National Health Service Breast Screening Programme Pathology External Quality Assurance Scheme (2016)
Journal Article
Rakha, E. A., Ahmed, M. A., Aleskandarany, M. A., Hodi, Z., Lee, A. H. S., Pinder, S. E., & Ellis, I. O. (2017). Diagnostic concordance of breast pathologists: lessons from the National Health Service Breast Screening Programme Pathology External Quality Assurance Scheme. Histopathology, 70(4), 632-642. doi:10.1111/his.13117

Optimized inducible shRNA and CRISPR/Cas9 platforms for in vitro studies of human development using hPSCs (2016)
Journal Article
Bertero, A., Pawlowski, M., Ortmann, D., Snijders, K., Yiangou, L., Cardoso de Brito, M., …Vallier, L. (2016). Optimized inducible shRNA and CRISPR/Cas9 platforms for in vitro studies of human development using hPSCs. Development, 143(23), doi:10.1242/dev.138081

Inducible loss of gene function experiments are necessary to uncover mechanisms underlying development, physiology and disease. However, current methods are complex, lack robustness and do not work in multiple cell types. Here we address these limita... Read More

Clinicopathological and functional significance of RECQL1 helicase in sporadic breast cancers (2016)
Journal Article
Arora, A., Parvathaneni, S., Aleskandarany, M. A., Agarwal, D., Ali, R., Abdel-Fatah, T., …Madhusudan, S. (2017). Clinicopathological and functional significance of RECQL1 helicase in sporadic breast cancers. Molecular Cancer Therapeutics, 16(1), doi:10.1158/1535-7163.MCT-16-0290

RECQL1, a key member of the RecQ family of DNA helicases, is required for DNA replication and DNA repair. Two recent studies have shown that germline RECQL1 mutations are associated with increased breast cancer susceptibility. Whether altered RECQL1... Read More

The efficacy and safety of continued hydroxycarbamide therapy versus switching to ruxolitinib in patients with polycythaemia vera: a randomized, double-blind, double-dummy, symptom study (RELIEF) (2016)
Journal Article
Mesa, R., Byrne, J., Vannucchi, A. M., Yacoub, A., Zachee, P., Garg, M., …Martino, B. (2017). The efficacy and safety of continued hydroxycarbamide therapy versus switching to ruxolitinib in patients with polycythaemia vera: a randomized, double-blind, double-dummy, symptom study (RELIEF). British Journal of Haematology, 176(1), 76-85. doi:10.1111/bjh.14382

The randomized, double‐blind, double‐dummy, phase 3b RELIEF trial evaluated polycythaemia vera (PV)‐related symptoms in patients who were well controlled with a stable dose of hydroxycarbamide (also termed hydroxyurea) but reported PV‐related symptom... Read More

Breast conservation in ductal carcinoma in situ (DCIS): what defines optimal margins? (2016)
Journal Article
Toss, M. S., Pinder, S. E., Green, A. R., Thomas, J., Morgan, D. A., Robertson, J. F., …Rakha, E. A. (2017). Breast conservation in ductal carcinoma in situ (DCIS): what defines optimal margins?. Histopathology, 70(5), 681-692. doi:10.1111/his.13116

The introduction of mammographic screening has resulted in a rise in the detection rate of ductal carcinoma in situ (DCIS), currently accounting for one‐fifth of screen‐detected breast cancers. Although 60–70% of DCIS are treated with breast‐conservi... Read More

SCIB1, a huIgG1 antibody DNA vaccination, combined with PD-1 blockade induced efficient therapy of poorly immunogenic tumors (2016)
Journal Article
Xue, W., Brentville, V. A., Symonds, P., Cook, K., Yagita, H., Metheringham, R. L., & Durrant, L. (in press). SCIB1, a huIgG1 antibody DNA vaccination, combined with PD-1 blockade induced efficient therapy of poorly immunogenic tumors. Oncotarget, 7(50), doi:10.18632/oncotarget.13070

Purpose: We have previously shown that supraoptimal signaling of high avidity T cells leads to high expression of PD-1 and inhibition of proliferation. This study was designed to see if this effect could be mitigated by combining a vaccine that stimu... Read More

Clinicopathological and molecular characteristics of Ku 70/80 expression in Nigerian breast cancer and its potential therapeutic implications (2016)
Journal Article
Agboola, A. O., Ebili, H. O., Iyawe, V. O., Banjo, A. A., Salami, B. A., Rakha, E. A., …Green, A. R. (2017). Clinicopathological and molecular characteristics of Ku 70/80 expression in Nigerian breast cancer and its potential therapeutic implications. Pathology - Research and Practice, 213(1), 27-33. doi:10.1016/j.prp.2016.10.005

Ku 70/80 is a regulator of the Non-Homologous End Joining (NHEJ) roles in clinicopathological features, and has prognostic significance in breast cancer (BC) in Caucasian populations. However, its significance in the Nigerian BC population, which is... Read More

Further evidence to support bimodality of oestrogen receptor expression in breast cancer (2016)
Journal Article
Muftah, A. A., Aleskandarany, M., Sonbul, S. N., Nolan, C. C., Diez Rodriguez, M., Caldas, C., …Rakha, E. A. (2017). Further evidence to support bimodality of oestrogen receptor expression in breast cancer. Histopathology, 70(3), 456-465. doi:10.1111/his.13089

Aims: Although oestrogen receptor (ER)‐negative breast cancers (BCs) do not respond to hormone therapy, the response of ER‐positive BCs is reported to be variable, which may suggest a dose‐dependent effect. The aim of this study was to assess the pat... Read More

High-dose therapy and autologous stem cell transplantation in patients with POEMS syndrome: a retrospective study of the Plasma Cell Disorder sub-committee of the Chronic Malignancy Working Party of the European Society for Blood & Marrow Transplantation (2016)
Journal Article
Cook, G., Iacobelli, S., van Biezen, A., Ziegkos, D., La Blond, V., Abraham, J., …Garderet, L. (2017). High-dose therapy and autologous stem cell transplantation in patients with POEMS syndrome: a retrospective study of the Plasma Cell Disorder sub-committee of the Chronic Malignancy Working Party of the European Society for Blood & Marrow Transplantation. Haematologica, 102(1), doi:10.3324/haematol.2016.148460

POEMS syndrome is a rare para-neoplastic syndrome secondary to a plasma cell dyscrasia. Effective treatment can control the diseaserelated symptom complex. We describe the clinical outcome of autologous stem cell transplantation for patients with POE... Read More

Adrenal cortical and chromaffin stem cells: Is there a common progeny related to stress adaptation? (2016)
Journal Article
Steenblock, C., de Celis, M. F. R., Androutsellis-Theotokis, A., Sue, M., Delgadillo Silva, L. F., Eisenhofer, G., …Bornstein, S. R. (2017). Adrenal cortical and chromaffin stem cells: Is there a common progeny related to stress adaptation?. Molecular and Cellular Endocrinology, 441, doi:10.1016/j.mce.2016.09.011

The adrenal gland is a highly plastic organ with the capacity to adapt the body homeostasis to different physiological needs. The existence of stem-like cells in the adrenal cortex has been revealed in many studies. Recently, we identified and charac... Read More

Mutational signatures in esophageal adenocarcinoma define etiologically distinct subgroups with therapeutic relevance (2016)
Journal Article
Secrier, M., Li, X., de Silva, N., Eldridge, M. D., Contino, G., Bornschein, J., …Grimmond, S. M. (2016). Mutational signatures in esophageal adenocarcinoma define etiologically distinct subgroups with therapeutic relevance. Nature Genetics, 48(10), doi:10.1038/ng.3659

Esophageal adenocarcinoma (EAC) has a poor outcome, and targeted therapy trials have thus far been disappointing owing to a lack of robust stratification methods. Whole-genome sequencing (WGS) analysis of 129 cases demonstrated that this is a heterog... Read More

Mertk on tumor macrophages is a therapeutic target to prevent tumor recurrence following radiation therapy (2016)
Journal Article
Crittenden, M. R., Baird, J., Malecka, A., Jackson, A. M., & Gough, M. J. (2016). Mertk on tumor macrophages is a therapeutic target to prevent tumor recurrence following radiation therapy. Oncotarget, 7(48), doi:10.18632/oncotarget.11823

Radiation therapy provides a means to kill large numbers of cancer cells in a controlled location resulting in the release of tumor-specific antigens and endogenous adjuvants. However, by activating pathways involved in apoptotic cell recognition and... Read More

Impact of tissue sampling on accuracy of Ki67 immunohistochemistry evaluation in breast cancer (2016)
Journal Article
Besusparis, J., Plancoulaine, B., Rasmusson, A., Augulis, R., Green, A. R., Ellis, I. O., …Laurinavicius, A. (2016). Impact of tissue sampling on accuracy of Ki67 immunohistochemistry evaluation in breast cancer. Diagnostic Pathology, 11(1), doi:10.1186/s13000-016-0525-z

Background: Gene expression studies have identified molecular subtypes of breast cancer with implications to chemotherapy recommendations. For distinction of these types, a combination of immunohistochemistry (IHC) markers, including proliferative ac... Read More

Prognostic significance of androgen receptor expression in invasive breast cancer: transcriptomic and protein expression analysis (2016)
Journal Article
Aleskandarany, M. A., Abduljabbar, R., Ashankyty, I., Elmouna, A., Jerjees, D., Ali, S., …Rakha, E. A. (2016). Prognostic significance of androgen receptor expression in invasive breast cancer: transcriptomic and protein expression analysis. Breast Cancer Research and Treatment, 159(2), 215-227. doi:10.1007/s10549-016-3934-5

Differential prognostic roles of Androgen Receptor (AR) have been proposed in breast cancer (BC) depending on tumour oestrogen receptor (ER) status. This study aimed to evaluate the prognostic and/or predictive significance of AR expression in invasi... Read More

A prebiotic galactooligosaccharide mixture reduces severity of hyperpnoea-induced bronchoconstriction and markers of airway inflammation (2016)
Journal Article
Williams, N. C., Johnson, M. A., Shaw, D. E., Spendlove, I., Vulevic, J., Sharpe, G. R., & Hunter, K. A. (2016). A prebiotic galactooligosaccharide mixture reduces severity of hyperpnoea-induced bronchoconstriction and markers of airway inflammation. British Journal of Nutrition, 116(5), 798-804. doi:10.1017/S0007114516002762

Gut microbes have a substantial influence on systemic immune function and allergic sensitisation. Manipulation of the gut microbiome through prebiotics may provide a potential strategy to influence the immunopathology of asthma. This study investigat... Read More

Can human pluripotent stem cell-derived cardiomyocytes advance understanding of muscular dystrophies? (2016)
Journal Article
Kalra, S., Montanaro, F., & Denning, C. (2016). Can human pluripotent stem cell-derived cardiomyocytes advance understanding of muscular dystrophies?. Journal of Neuromuscular Diseases, 3(3), doi:10.3233/JND-150133

Muscular dystrophies (MDs) are clinically and molecularly a highly heterogeneous group of single-gene disorders that primarily affect striated muscles. Cardiac disease is present in several MDs where it is an important contributor to morbidity and mo... Read More