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Characterisation of Listeria monocytogenes isolates from cattle using a bovine caruncular epithelial cell model (2020)
Journal Article
Blanchard, A. M., Billenness, R., Warren, J., Glanvill, A., Roden, W., Drinkall, E., …Tötemeyer, S. (2020). Characterisation of Listeria monocytogenes isolates from cattle using a bovine caruncular epithelial cell model. Heliyon, 6(7), Article e04476. https://doi.org/10.1016/j.heliyon.2020.e04476

© 2020 Listeria monocytogenes is an important foodborne pathogen in human and veterinary health, causing significant morbidity and mortality including abortion. It has a particular tropism for the gravid uterus, however, the route of infection in rep... Read More about Characterisation of Listeria monocytogenes isolates from cattle using a bovine caruncular epithelial cell model.

The development and use of Actiphage® to detect viable mycobacteria from bovine tuberculosis and Johne’s disease-infected animals (2019)
Journal Article
Swift, B. M., Meade, N., Barron, E. S., Bennett, M., Perehenic, T., Hughes, V., …Rees, C. E. (2020). The development and use of Actiphage® to detect viable mycobacteria from bovine tuberculosis and Johne’s disease-infected animals. Microbial Biotechnology, 13(3), 738-746. https://doi.org/10.1111/1751-7915.13518

© 2019 The Authors. Microbial Biotechnology published by John Wiley & Sons Ltd and Society for Applied Microbiology. Here, we describe the development of a method that exploits bacteriophage D29 as a lysis agent for efficient DNA extraction from lo... Read More about The development and use of Actiphage® to detect viable mycobacteria from bovine tuberculosis and Johne’s disease-infected animals.

Evaluation of the limitations and methods to improve rapid phage-based detection of viable Mycobacterium avium subsp. paratuberculosis in the blood of experimentally infected cattle (2016)
Journal Article
Swift, B. M., Huxley, J., Plain, K. M., Begg, D. J., de Silva, K., Purdie, A. C., …Rees, C. (2016). Evaluation of the limitations and methods to improve rapid phage-based detection of viable Mycobacterium avium subsp. paratuberculosis in the blood of experimentally infected cattle. BMC Veterinary Research, 12, Article 115. https://doi.org/10.1186/s12917-016-0728-2

Background Disseminated infection and bacteraemia is an underreported and under-researched aspect of Johne’s disease. This is mainly due to the time it takes for Mycobacterium avium subsp. paratuberculosis (MAP) to grow and lack of sensitivity of... Read More about Evaluation of the limitations and methods to improve rapid phage-based detection of viable Mycobacterium avium subsp. paratuberculosis in the blood of experimentally infected cattle.

Evidence of Mycobacterium tuberculosis Complex bacteraemia in intradermal skin test positive cattle detected using phage-RPA (2016)
Journal Article
Swift, B., Convery, T., & Rees, C. (2016). Evidence of Mycobacterium tuberculosis Complex bacteraemia in intradermal skin test positive cattle detected using phage-RPA. Virulence, 7(7), 779-788. https://doi.org/10.1080/21505594.2016.1191729

Bovine tuberculosis is a zoonotic infectious disease caused by Mycobacterium bovis 19 that affects cattle and can cause tuberculosis in a range of wildlife animals. A 20 bacteriophage-based method combined with PCR (phage-PCR) has been recently used... Read More about Evidence of Mycobacterium tuberculosis Complex bacteraemia in intradermal skin test positive cattle detected using phage-RPA.

Detection of viable Mycobacterium avium subspecies paratuberculosis in powdered infant formula by phage-PCR and confirmed by culture (2015)
Journal Article
Rees, C., Botsaris, G., Swift, B. M., Slana, I., Liapi, M., Christodoulou, M., …Christodoulou, V. (2016). Detection of viable Mycobacterium avium subspecies paratuberculosis in powdered infant formula by phage-PCR and confirmed by culture. International Journal of Food Microbiology, 216, 91-94. https://doi.org/10.1016/j.ijfoodmicro.2015.09.011

Factors affecting phage D29 infection: a tool to investigate different growth states of mycobacteria (2014)
Journal Article
Swift, B., Gerrard, Z., Huxley, J., & Rees, C. (2014). Factors affecting phage D29 infection: a tool to investigate different growth states of mycobacteria. PLoS ONE, 9(9), Article e106690. https://doi.org/10.1371/journal.pone.0106690

Bacteriophages D29 and TM4 are able to infect a wide range of mycobacteria, including pathogenic and non pathogenic species. Successful phage infection of both fast- and slow-growing mycobacteria can be rapidly detected using the phage amplification... Read More about Factors affecting phage D29 infection: a tool to investigate different growth states of mycobacteria.