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UDP-sugars activate P2Y 14 receptors to mediate vasoconstriction of the porcine coronary artery (2017)
Journal Article
Abbas, Z. S., Latif, M. L., Dovlatova, N., Fox, S. C., Heptinstall, S., Dunn, W. R., & Ralevic, V. (2018). UDP-sugars activate P2Y 14 receptors to mediate vasoconstriction of the porcine coronary artery. Vascular Pharmacology, 103-105, https://doi.org/10.1016/j.vph.2017.12.063

Aims UDP-sugars can act as extracellular signalling molecules, but relatively little is known about their cardiovascular actions. The P2Y14 receptor is a Gi/o-coupled receptor which is activated by UDP-glucose and related sugar nucleotides. In thi... Read More about UDP-sugars activate P2Y 14 receptors to mediate vasoconstriction of the porcine coronary artery.

Gas-free calibrated fMRI with a correction for vessel-size sensitivity (2017)
Journal Article
Berman, A. J., Mazerolle, E. L., MacDonald, M. E., Blockley, N. P., Luh, W., & Pike, G. B. (2018). Gas-free calibrated fMRI with a correction for vessel-size sensitivity. NeuroImage, 169, 176-188. https://doi.org/10.1016/j.neuroimage.2017.12.047

Calibrated functional magnetic resonance imaging (fMRI) is a method to independently measure the metabolic and hemodynamic contributions to the blood oxygenation level dependent (BOLD) signal. This technique typically requires the use of a respirator... Read More about Gas-free calibrated fMRI with a correction for vessel-size sensitivity.

A non-imaging high throughput approach to chemical library screening at the unmodified adenosine-A3 receptor in living cells (2017)
Journal Article
Arruda, M. A., Stoddart, L. A., Gherbi, K., Briddon, S. J., Kellam, B., & Hill, S. J. (in press). A non-imaging high throughput approach to chemical library screening at the unmodified adenosine-A3 receptor in living cells. Frontiers in Pharmacology, 8(908), https://doi.org/10.3389/fphar.2017.00908

Recent advances in fluorescent ligand technology have enabled the study of G protein-coupled receptors in their native environment without the need for genetic modification such as addition of N-terminal fluorescent or bioluminescent tags. Here, we h... Read More about A non-imaging high throughput approach to chemical library screening at the unmodified adenosine-A3 receptor in living cells.

A Cdk1 phosphomimic mutant of MCAK impairs microtubule end recognition (2017)
Other
Belsham, H. R., & Friel, C. T. (2017). A Cdk1 phosphomimic mutant of MCAK impairs microtubule end recognition

The microtubule depolymerising kinesin-13, MCAK, is phosphorylated at residue T537 by Cdk1. This is the only known phosphorylation site within MCAK’s motor domain. To understand the impact of phosphorylation by Cdk1 microtubule depolymerisation activ... Read More about A Cdk1 phosphomimic mutant of MCAK impairs microtubule end recognition.

A Cdk1 phosphomimic mutant of MCAK impairs microtubule end recognition (2017)
Journal Article
Belsham, H. R., & Friel, C. T. (2017). A Cdk1 phosphomimic mutant of MCAK impairs microtubule end recognition. PeerJ, 5, Article e4034. https://doi.org/10.7717/peerj.4034

The microtubule depolymerising kinesin-13, MCAK, is phosphorylated at residue T537 by Cdk1. This is the only known phosphorylation site within MCAK’s motor domain. To understand the impact of phosphorylation by Cdk1 on microtubule depolymerisation ac... Read More about A Cdk1 phosphomimic mutant of MCAK impairs microtubule end recognition.

13C-assisted metabolic flux analysis to investigate heterotrophic and mixotrophic metabolism in Cupriavidus necator H16 (2017)
Journal Article
Alagesan, S., Minton, N. P., & Malys, N. (2018). 13C-assisted metabolic flux analysis to investigate heterotrophic and mixotrophic metabolism in Cupriavidus necator H16. Metabolomics, 14(9), https://doi.org/10.1007/s11306-017-1302-z

Introduction. Cupriavidus necator H16 is a gram-negative bacterium, capable of lithoautotrophic growth by utilizing hydrogen as an energy source and fixing carbon dioxide (CO2) through Calvin-Benson-Bassham (CBB) cycle. The potential to utilize synth... Read More about 13C-assisted metabolic flux analysis to investigate heterotrophic and mixotrophic metabolism in Cupriavidus necator H16.

Glycaemic, gastrointestinal and appetite responses to breakfast porridges from ancient cereal grains: a MRI pilot study in healthy humans (2017)
Journal Article
Alyami, J., Ladd, N., Pritchard, S. E., Hoad, C. L., Abdul Sultan, A., Spiller, R. C., …Taylor, M. A. (2017). Glycaemic, gastrointestinal and appetite responses to breakfast porridges from ancient cereal grains: a MRI pilot study in healthy humans. Food Research International, https://doi.org/10.1016/j.foodres.2017.11.071

Cereal grain based porridges are commonly consumed throughout the world. Whilst some data are available for varieties that are popular in the Western world such as oats and rye, other ‘ancient’ grains used in the East and in Africa such as millets ar... Read More about Glycaemic, gastrointestinal and appetite responses to breakfast porridges from ancient cereal grains: a MRI pilot study in healthy humans.

Cancer chemotherapy in early life significantly alters the maturation of pain processing (2017)
Journal Article
Hathway, G. J., Murphy, E., Lloyd, J., Greenspon, C. M., & Hulse, R. P. (2017). Cancer chemotherapy in early life significantly alters the maturation of pain processing. Neuroscience, https://doi.org/10.1016/j.neuroscience.2017.11.032

Advances in paediatric cancer treatment have led to a ten year survival rate greater than 75%. Platinum-based chemotherapies (e.g.cisplatin) induce peripheral sensory neuropathy in adult and paedriatric cancer patients. The period from birth through... Read More about Cancer chemotherapy in early life significantly alters the maturation of pain processing.

Applying asymptotic methods to synthetic biology: modelling the reaction kinetics of the mevalonate pathway (2017)
Journal Article
Dalwadi, M. P., Garavaglia, M., Webb, J. P., King, J. R., & Minton, N. P. (2018). Applying asymptotic methods to synthetic biology: modelling the reaction kinetics of the mevalonate pathway. Journal of Theoretical Biology, 439, https://doi.org/10.1016/j.jtbi.2017.11.022

The mevalonate pathway is normally found in eukaryotes, and allows for the production of isoprenoids, a useful class of organic compounds. This pathway has been successfully introduced to Escherichia coli, enabling a biosynthetic production route for... Read More about Applying asymptotic methods to synthetic biology: modelling the reaction kinetics of the mevalonate pathway.

Candida albicans Hap43 domains are required under iron starvation but not excess (2017)
Journal Article
Skrahina, V., Brock, M., Hube, B., & Brunke, S. (2017). Candida albicans Hap43 domains are required under iron starvation but not excess. Frontiers in Microbiology, 8, Article 2388. https://doi.org/10.3389/fmicb.2017.02388

Iron availability is a central factor in infections, since iron is a critical micronutrient for all living organisms. The host employs both iron limitation and toxicity strategies to control microbial growth, and successful pathogens are able to tigh... Read More about Candida albicans Hap43 domains are required under iron starvation but not excess.

Whole-exome sequencing of the BDR cohort: evidence to support the role of the PILRA gene in Alzheimer’s disease (2017)
Journal Article
Patel, T., Brookes, K. J., Turton, J., Chaudhury, S., Guetta-Baranes, T., Guerreiro, R., …Morgan, K. (2019). Whole-exome sequencing of the BDR cohort: evidence to support the role of the PILRA gene in Alzheimer’s disease. Neuropathology and Applied Neurobiology, 44(5), 506-521. https://doi.org/10.1111/nan.12452

Aim: Late-onset Alzheimer’s disease (LOAD) accounts for 95% of all Alzheimer’s cases and is genetically complex in nature. Overlapping clinical and neuropathological features between AD, FTD and Parkinson’s disease highlight the potential role of gen... Read More about Whole-exome sequencing of the BDR cohort: evidence to support the role of the PILRA gene in Alzheimer’s disease.

The effect of weak inertia in rotating high-aspect-ratio vessel bioreactors (2017)
Journal Article
Dalwadi, M. P., Chapman, S. J., Oliver, J. M., & Waters, S. L. (2018). The effect of weak inertia in rotating high-aspect-ratio vessel bioreactors. Journal of Fluid Mechanics, 835, 674-720. https://doi.org/10.1017/jfm.2017.760

One method to grow artificial body tissue is to place a porous scaffold seeded with cells, known as a tissue construct, into a rotating bioreactor filled with a nutrient-rich fluid. The flow within the bioreactor is affected by the movement of the co... Read More about The effect of weak inertia in rotating high-aspect-ratio vessel bioreactors.

Regulation of human feto-placental endothelial barrier integrity by vascular endothelial growth factors: competitive interplay between VEGF-A165a, VEGF-A165b, PIGF and VE-cadherin (2017)
Journal Article
Pang, V., Bates, D. O., & Leach, L. (2017). Regulation of human feto-placental endothelial barrier integrity by vascular endothelial growth factors: competitive interplay between VEGF-A165a, VEGF-A165b, PIGF and VE-cadherin. Clinical Science, 131(23), 2763-2775. https://doi.org/10.1042/CS20171252

The human placenta nourishes and protects the developing fetus whilst influencing maternal physiology for fetal advantage. It expresses several members of the VEGF family including the pro-angiogenic/pro-permeability VEGF-A165a isoform, the anti-angi... Read More about Regulation of human feto-placental endothelial barrier integrity by vascular endothelial growth factors: competitive interplay between VEGF-A165a, VEGF-A165b, PIGF and VE-cadherin.

NAD-biosynthetic enzyme NMNAT1 reduces early behavioral impairment in the htau mouse model of tauopathy (2017)
Journal Article
Rossi, F., Geiszler, P. C., Meng, W., Barron, M., Prior, M., Herd-Smith, A., …Conforti, L. (2018). NAD-biosynthetic enzyme NMNAT1 reduces early behavioral impairment in the htau mouse model of tauopathy. Behavioural Brain Research, 339, https://doi.org/10.1016/j.bbr.2017.11.030

NAD metabolism and the NAD biosynthetic enzymes nicotinamide nucleotide adenylyltransferases (NMNATs) are thought to play a key neuroprotective role in tauopathies, including Alzheimer’s disease. Here, we investigated whether modulating the expressio... Read More about NAD-biosynthetic enzyme NMNAT1 reduces early behavioral impairment in the htau mouse model of tauopathy.

Acetylation of TBX5 by KAT2B and KAT2A regulates heart and limb development (2017)
Journal Article
Ghosh, T. K., Aparicio-Sánchez, J. J., Buxton, S., Ketley, A., Mohamed, T., Rutland, C. S., …Brook, J. D. (2018). Acetylation of TBX5 by KAT2B and KAT2A regulates heart and limb development. Journal of Molecular and Cellular Cardiology, 114, 185-198. https://doi.org/10.1016/j.yjmcc.2017.11.013

© 2017 Elsevier Ltd TBX5 plays a critical role in heart and forelimb development. Mutations in TBX5 cause Holt-Oram syndrome, an autosomal dominant condition that affects the formation of the heart and upper-limb. Several studies have provided signif... Read More about Acetylation of TBX5 by KAT2B and KAT2A regulates heart and limb development.

Single-gene speciation: mating and gene flow between mirror-image snails (2017)
Journal Article
Richards, P. M., Morii, Y., Kimura, K., Hirano, T., Chiba, S., & Davison, A. (2017). Single-gene speciation: mating and gene flow between mirror-image snails. Evolution Letters, 1(6), https://doi.org/10.1002/evl3.31

Variation in the shell coiling, or chirality, of land snails provides an opportunity to investigate the potential for “single-gene” speciation, because mating between individuals of opposite chirality is believed not possible if the snails mate in a... Read More about Single-gene speciation: mating and gene flow between mirror-image snails.

Multi-timescale analysis of a metabolic network in synthetic biology: a kinetic model for 3-hydroxypropionic acid production via beta-alanine (2017)
Journal Article
Dalwadi, M. P., King, J. R., & Minton, N. P. (2018). Multi-timescale analysis of a metabolic network in synthetic biology: a kinetic model for 3-hydroxypropionic acid production via beta-alanine. Journal of Mathematical Biology, 77(1), 165-199. https://doi.org/10.1007/s00285-017-1189-3

A biosustainable production route for 3-hydroxypropionic acid (3HP), an important platform chemical, would allow 3HP to be produced without using fossil fuels. We are interested in investigating a potential biochemical route to 3HP from pyruvate thro... Read More about Multi-timescale analysis of a metabolic network in synthetic biology: a kinetic model for 3-hydroxypropionic acid production via beta-alanine.

Mathematical modelling of chemical agent removal by reaction with an immiscible cleanser (2017)
Journal Article
Dalwadi, M. P., O'Kiely, D., Thomson, S., Khaleque, T., & Hall, C. (in press). Mathematical modelling of chemical agent removal by reaction with an immiscible cleanser. SIAM Journal on Applied Mathematics, 77(6), https://doi.org/10.1137/16M1101647

When a hazardous chemical agent has soaked into a porous medium, such as concrete, it can be difficult to neutralise. One removal method is chemical decontamination, where a cleanser is applied to react with and neutralise the agent, forming less har... Read More about Mathematical modelling of chemical agent removal by reaction with an immiscible cleanser.

An integrated dyspepsia module for first year Pharmacy students: a flexible and generic template for integrating science with clinical and professional practice (2017)
Journal Article
De Matteis, C. I., Randall, M. D., Harvey, E. J., Morris, A., Winkler, G. S., & Boardman, H. F. (2019). An integrated dyspepsia module for first year Pharmacy students: a flexible and generic template for integrating science with clinical and professional practice. American Journal of Pharmaceutical Education, 83(1), Article 6508. https://doi.org/10.5688/ajpe6508

Objective: To design an integrated dyspepsia module for first year pharmacy undergraduates, which combines clinical and professional practice with fundamental sciences, in five different science subject areas, as a prototype for future disease- or sy... Read More about An integrated dyspepsia module for first year Pharmacy students: a flexible and generic template for integrating science with clinical and professional practice.