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Photoperiodic changes in adiposity increase sensitivity of female Siberian hamsters to systemic VGF derived peptide TLQP-21 (2019)
Journal Article
Lisci, C., Lewis, J. E., Daniel, Z. C., Stevenson, T. J., Monnier, C., Marshall, H. J., …Jethwa, P. H. (2019). Photoperiodic changes in adiposity increase sensitivity of female Siberian hamsters to systemic VGF derived peptide TLQP-21. PLoS ONE, 14(8), Article e0221517. https://doi.org/10.1371/journal.pone.0221517

TLQP-21, a peptide encoded by the highly conserved vgf gene, is expressed in neuroendocrine cells and has been the most prominent VGF-derived peptide studied in relation to control of energy balance. The recent discovery that TLQP-21 is the natural a... Read More about Photoperiodic changes in adiposity increase sensitivity of female Siberian hamsters to systemic VGF derived peptide TLQP-21.

Interscapular and Perivascular Brown Adipose Tissue Respond Differently to a Short-Term High-Fat Diet (2019)
Journal Article
Aldiss, P., Lewis, J., Boocock, D., Miles, A., Bloor, I., Ebling, F., …Symonds, M. (2019). Interscapular and Perivascular Brown Adipose Tissue Respond Differently to a Short-Term High-Fat Diet. Nutrients, 11(5), Article 1065. https://doi.org/10.3390/nu11051065

© 2019 by the authors. Licensee MDPI, Basel, Switzerland. Brown adipose tissue (BAT) function may depend on its anatomical location and developmental origin. Interscapular BAT (iBAT) regulates acute macronutrient metabolism, whilst perivascular BAT (... Read More about Interscapular and Perivascular Brown Adipose Tissue Respond Differently to a Short-Term High-Fat Diet.

Effect of sodium 4-phenylbutyrate on clenbuterol-mediated muscle growth (2018)
Journal Article
Brown, D. M., Jones, S., Daniel, Z. C., Brearley, M. C., Lewis, J. E., Ebling, F. J., …Brameld, J. M. (2018). Effect of sodium 4-phenylbutyrate on clenbuterol-mediated muscle growth. PLoS ONE, 13(7), Article e0201481. https://doi.org/10.1371/journal.pone.0201481

Previously, we highlighted induction of an integrated stress response (ISR) gene program in skeletal muscle of pigs treated with a beta-adrenergic agonist. Hence we tested the hypothesis that the ER-stress inhibitor, sodium 4-phenylbutyrate (PBA), wo... Read More about Effect of sodium 4-phenylbutyrate on clenbuterol-mediated muscle growth.

Antibody mediated targeting of the FGFR1c isoform increases glucose uptake in white and brown adipose tissue in male mice (2017)
Journal Article
Lewis, J. E., Samms, R. J., Cooper, S., Luckett, J. C., Perkins, A. C., Dunbar, J. D., …Tsintzas, K. (2017). Antibody mediated targeting of the FGFR1c isoform increases glucose uptake in white and brown adipose tissue in male mice. Endocrinology, 158(10), 3090–3096. https://doi.org/10.1210/en.2017-00591

The increased prevalence of obesity and its cardiometabolic implications demonstrates the imperative to identify novel therapeutic targets able to effect meaningful metabolic changes in this population. Antibody-mediated targeting of fibroblast growt... Read More about Antibody mediated targeting of the FGFR1c isoform increases glucose uptake in white and brown adipose tissue in male mice.

FGF21 is an insulin-dependent postprandial hormone in adult humans (2017)
Journal Article
Samms, R. J., Lewis, J. E., Norton, L., Stephens, F. B., Gaffney, C. J., Butterfield, T., …Tsintzas, K. (in press). FGF21 is an insulin-dependent postprandial hormone in adult humans. Journal of Clinical Endocrinology and Metabolism, https://doi.org/10.1210/jc.2017-01257

Context: Fibroblast growth factor 21 (FGF21) secretion has been shown to respond directly to carbohydrate consumption, with glucose, fructose and sucrose all reported to increase plasma levels of FGF21 in rodents and humans. However, carbohydrate con... Read More about FGF21 is an insulin-dependent postprandial hormone in adult humans.

Reduced adiposity attenuates FGF21 mediated metabolic improvements in the Siberian hamster (2017)
Journal Article
Lewis, J. E., Samms, R. J., Cooper, S., Luckett, J. C., Perkins, A. C., Adams, A. C., …Ebling, F. J. (in press). Reduced adiposity attenuates FGF21 mediated metabolic improvements in the Siberian hamster. Scientific Reports, 7(1), Article 4238. https://doi.org/10.1038/s41598-017-03607-x

FGF21 exerts profound metabolic effects in Siberian hamsters exposed to long day (LD) photoperiods that increase appetite and adiposity, however these effects are attenuated in short day (SD) animals that display hypophagia and reduced adiposity. The... Read More about Reduced adiposity attenuates FGF21 mediated metabolic improvements in the Siberian hamster.

Hypothalamic over-expression of VGF in the Siberian hamster increases energy expenditure and reduces body weight gain (2017)
Journal Article
Lewis, J. E., Brameld, J. M., Hill, P., Cocco, C., Noli, B., Ferri, G., …Jethwa, P. (2017). Hypothalamic over-expression of VGF in the Siberian hamster increases energy expenditure and reduces body weight gain. PLoS ONE, 12, Article e01727. https://doi.org/10.1371/journal.pone.0172724

VGF (non-acronymic) was first highlighted to have a role in energy homeostasis through experiments involving dietary manipulation in mice. Fasting increased VGF mRNA in the Arc and levels were subsequently reduced upon refeeding. This anabolic role f... Read More about Hypothalamic over-expression of VGF in the Siberian hamster increases energy expenditure and reduces body weight gain.

Antibody-mediated inhibition of the FGFR1c isoform induces a catabolic lean state in Siberian hamsters (2015)
Journal Article
Samms, R. J., Lewis, J. E., Lory, A., Fowler, M. J., Cooper, S., Warner, A., …Ebling, F. J. (2015). Antibody-mediated inhibition of the FGFR1c isoform induces a catabolic lean state in Siberian hamsters. Current Biology, 25(22), https://doi.org/10.1016/j.cub.2015.10.010

Hypothalamic tanycytes are considered to function as sensors of peripheral metabolism [1]. To facilitate this role, they express a wide range of receptors, including fibroblast growth factor receptor 1 (FGFR1). Using a monoclonal antibody (IMC-H7) th... Read More about Antibody-mediated inhibition of the FGFR1c isoform induces a catabolic lean state in Siberian hamsters.

The use of viral 2A sequence for the simultaneous over-expression of both the vgf gene and enhanced green fluorescent protein eGFP (2015)
Journal Article
Lewis, J. E., Brameld, J. M., Hill, P., Barrett, P., Ebling, F. J., & Jethwa, P. (in press). The use of viral 2A sequence for the simultaneous over-expression of both the vgf gene and enhanced green fluorescent protein eGFP. Journal of Neuroscience Methods, 256, https://doi.org/10.1016/j.jneumeth.2015.08.013

Introduction: The viral 2A sequence has become an attractive alternative to the traditional internal ribosomal entry site (IRES) for simultaneous over-expression of two genes and in combination with recombinant adeno-associated viruses (rAAV) has bee... Read More about The use of viral 2A sequence for the simultaneous over-expression of both the vgf gene and enhanced green fluorescent protein eGFP.